Vetiver Research Articles

Genetic variability and stability pattern in vetiver (Chrysopogon zizanioides (L.) Roberty)

The vetiver (Chrysopogon zizanioides (L.) Roberty) essential oil is obtained from its roots by hydro- or steam distillation. Its oil is used in cosmetics, perfumery and food industries, deodorants, soaps, cold drinks, and toilet articles. The present investigations' objective was to estimate essential oil yielding stability and the identification of environment-specific vetiver genotypes. Testing of genotypes under different years was done using additive main effect and multiplicative interaction model, genotype main effect with the genotype-by-environment interaction, and biplot statistical models for the stability of the essential oil yield. The results from both models showed that the best stable genotypes for the high essential oil were G 10, G 12, G 14, and G 15, respectively. The essential oil of the genotypes, G 10, G 14, and G 15 were rich for khusimol, Khusol, khusimone and khusian-2-ol in G 10; khusilal, α-cedrenal, α-cedrenol, khusol, khusitone in G 14, and khusilal, khusimol, α-cedrenal, α-cedrenol,khusol, khusitone in G 15 along with minor compounds having diverse aromatic characters. The two new compounds, namely, α-cedrenal and α-cedrenol were identified first in the Indian vetiver, while the line G 12 possessed khusilal and khusol as the major compounds. Therefore, these genotypes of vetiver are recommended for commercial exploitation.

PENGARUH ABU TERBANG BATUBARA TERHADAP SIFAT KIMIA TANAH DAN SERAPAN TIMBAL (Pb) OLEH TANAMAN AKAR WANGI (Vetiveria zizanioides L.)

Coal fly ash is a soil amendment that can be used to improve soil productivity because coal fly ash contains essential nutrients needed by plants. However, coal fly ash also contains heavy metals. This study was at a time to explore the effect of coal fly ash on soil chemical properties and lead uptake by vetiver plant (Vetiveria zizanioides L.). Treatments tested were applications of six proportion levels (0, 2.5, 5., 10, 15, and 30% by weight). The seven treatments were arranged in a completely randomized design with three replicates. The result showed that the coal fly ash had a significant effect on soil pH, total-P, total-N, total-K and Pb. The value of the Pb translocation factor from the roots to the shoot of the vetiver plant was relatively low, so that the vetiver plant was not suitable for use in phytoextraction of Pb from Pb-contaminated soil.

Treatment of university campus wastewaters by anaerobic reactor and multi-stage constructed wetlands

Sustainable and decentralized sanitation is essential for a continental country like Brazil to mitigate its untreated sewage liabilities. This may be directly related to the constructed wetlands (CWs), since these systems are considered clean technology alternatives for the treatment of wastewaters. So, the present research investigated the treatment of university campus wastewaters by a hybrid system composed of: anaerobic reactors (AR) + vertical flow constructed wetlands (VFCW) + floating treatment wetlands (FTW) + horizontal subsurface flow constructed wetlands (HSSFCW). Effluents were treated in batch mode, with a loading time of 1 h, a flow after preliminary treatment of 1.6 m3 h−1 to load the system and hydraulic detention time of 3 days for the AR, and 7 days for each CW unit. CW were planted with 4 different macrophytes: Hymenachne grumosa, Vetiveria zizanoides, Papyrus and Lemna minor sp. The obtained results showed removal rates of 67 % for COD, 53 % for BOD5, 94 % for Total N, 90 % for DOC, 98 % for turbidity and of 5log for E. coli. Almost all the investigated parameters of the treated wastewaters were in accordance the emission limits established by current Brazilian legislation. Moreover, the good performance of the hybrid system brings new reuse possibilities for the treated wastewaters, so that they would be adequate to be reused in orchards, cereals, forages, pastures for livestock and other crops through runoff or by punctual irrigation system.

Back to the Roots-An Overview of the Chemical Composition and Bioactivity of Selected Root-Essential Oils.

Since ancient times, plant roots have been widely used in traditional medicine for treating various ailments and diseases due to their beneficial effects. A large number of studies have demonstrated that—besides their aromatic properties—their biological activity can often be attributed to volatile constituents. This review provides a comprehensive overview of investigations into the chemical composition of essential oils and volatile components obtained from selected aromatic roots, including Angelica archangelica, Armoracia rusticana, Carlina sp., Chrysopogon zizanioides, Coleus forskohlii, Inula helenium, Sassafras albidum, Saussurea costus, and Valeriana officinalis. Additionally, their most important associated biological impacts are reported, such as anticarcinogenic, antimicrobial, antioxidant, pesticidal, and other miscellaneous properties. Various literature and electronic databases—including PubMed, ScienceDirect, Springer, Scopus, Google Scholar, and Wiley—were screened and data was obtained accordingly. The results indicate the promising properties of root-essential oils and their potential as a source for natural biologically active products for flavor, pharmaceutical, agricultural, and fragrance industries. However, more research is required to further establish the mechanism of action mediating these bioactivities as well as essential oil standardization because the chemical composition often strongly varies depending on external factors.

Effects of Chrysopogon zizanioides root biomass and plant age on hydro-mechanical behavior of root-permeated soils

Effects of Chrysopogon zizanioides root biomass and plant age on hydro-mechanical behavior of root-permeated soils

Multi-years/environmental evaluation for high photosynthetic, bio-efficient and essential oil genotypes selection in the breeding of vetiver (Chrysopogon zizanioides (L.) Roberty) crop

ABSTRACT The stability and association parameters were quantified through a study of the seven economic physiological traits in the vetiver. This study’s objectives were to estimate the genotypes response over the years of interaction and the selection of stable and photosynthetic efficient lines of vetiver. The path analysis indicated the highest direct contributions to the yield of essential oil were made by transpiration rate (0.189) and essential oil percent (0.182) under the residual effect (R = 0.953). The stability models identified seven stable lines for oil yield and three highly stable lines for the high photosynthesis rate. The β-vetivenene, ��-cedrenal, ��-cedrenol, khusilal, khusol, khusimone, khusimol, and khusinol were the major oil components in selected genotypes. Based on the selection parameters, the genotypes K-59, K-42, K-22, K-46, K-2, K-60, K-30 for high oil yield, and K-12, K-58, K-14, K-45 for the photosynthesis rate are recommended for large scale cultivation.

IN VITRO CYTOTOXICITY STUDIES ON ISOLATED AND PURIFIED COMPOUNDS FROM CHRYSOPOGON ZIZANIOIDES CHLOROFORM EXTRACT ON VERO AND HCT-116 CANCER CELL LINES

The main objective of this study is to investigate the cytotoxic potential of four puried compounds (compounds 1,2,3 and 4) from medicinal plant Chrysopogon zizanioides L were investigated. Successive solvent extraction of Chrysopogon zizanioides in chloroform was done. The extract were tested against the normal cell lines (Vero) human colorectal cancer cell lines (HCT-116) using the thiazolyl blue test (MTT) assay. The chloroform fractions of Chrysopogon zizanioides had shown the signicant results against Vero and HCT-116 tumor cell lines. The IC50concentration of the selected puried compounds C1, C2, C3, and C4 against HCT 116 were 79.06 µg/ml, 84.13 µg/ml, 94.36 µg/ml and 68.47µg/ml, respectively. Whereas the IC50concentration of the selected puried compounds C1, C2, C3, and C4 against Vero were 130.30µg/ml, 157.36µg/ml, 160.48µg/ml and 255.28µg/ml, respectively. Among all the tested compounds, compound four can be considered as potential sources of anti-cancer compounds. Additional studies are necessary for more extensive biological evaluations.

The Treatment of Acid Mine Drainage Using Vertically Flowing Wetland: Insights into the Fate of Chemical Species

In this study, the treatment of acid mine drainage (AMD) using vertically flowing wetland was explored. The wetland was enriched with Vetiveria zizanioides as a decontaminating media and soil as the substrate. Water was percolated through the substrate and the throughput samples were collected and characterized every five days for a period of 30 days. The obtained results revealed a tolerant index of 1.03 for Vetiveria zizanioides, and a net reduction of metals and sulfate. The removal efficacy of chemical species was observed to obey the following order: Fe (71.25%) > Zn (70.40%) > Mn (62%) > Al (56.68%)> SO42− (55.18%) > Ni (35%) > Cu (18.83%). The removal of chemical species was further aided by the used substrate, and this could be attributed to the accumulation of chemical species on the soil through precipitation, adsorption, and phyto-retention. As such, it could be deduced that the substrate plays a significant role in the removal of metals, while the grass and external factors accounted for the rest of the chemical species attenuation. The translocation assessment revealed that the distribution of chemical species was observed to be predominant in the roots, except manganese, which was transferred in the shoot (67%). The XRF, XRD, FTIR, and SEM-EDS analysis revealed the presence of AMD chemical species in the substrate and the grass components, hence confirming that the plants are playing a huge role in the removal of contaminants from AMD. The PH REdox EQuilibrium (in C language) (PHREEQC) geochemical model confirm that metals existed as di-and-trivalent complexes in AMD. Lastly, available metals were precipitated as metals hydroxides and oxy-hydrosulfates by the substrate. In light of the obtained results, vertically flowing wetland could be used for the passive treatment of AMD, and it will play a huge role in active and abandoned mines. However, prolonged assessment should be undertaken to understand its performance over a notable period of time.

Impact of grass traits on the transport path and retention efficiency of nitrate nitrogen in vegetation filter strips

Vegetation filter strips (VFS) have been shown to effectively intercept water flow and remove nitrogen. Studies of the vegetation effects on water flow and nitrogen transport are typically studied based on qualitative analyses with species, layouts, growth stage, seasonal changes, and other vegetation conditions and did not considered quantification based on plant traits. In this study, the transport path and retention efficiency of nitrate nitrogen (NO3-N) in VFSs are investigated from the view of the plant specific trait effects by simulating runoff experiments using three grassed VFSs (centipede grass, tall fescue, and vetiver grass), as well as a bare VFS under different slope gradients (2%, 7%, and 12% slope gradients). The primary grass traits (stem spacing, root depth, root length density, and nitrogen total uptake) were measured, and the responses of water flow and NO3-N loss above and below ground to those were quantitatively assessed using the grey correlation analysis method. Results indicated that grasses and slope gradient significantly influenced the water flow and NO3-N loss in general, except the NO3-N loss concentration of the surface flow, in which the tall fescue VFS showed the highest NO3-N total retention rates under each slope condition. Considering the impact of specific grass traits, stem density and root length density has the greatest effects on the surface flow and the subsurface flow respectively, while the effect of roots was lower than that of stems on NO3-N loss below ground. Additionally, the NO3-N loss mass in the surface and subsurface flow were mostly related to the water flow volume and the NO3-N loss concentration respectively. This indicated that grass traits that reduce water flow should be considered more to control the NO3-N loss mass above ground, whereas the grass traits reducing NO3-N loss concentration should be considered more to control the NO3-N loss mass below ground.

Grass/Soil Concentration Ratios (CRs) of Natural 226Ra, 232Th, and 40K in Vetiveria zizanioides (Vetiver Grass) in Thailand

This work investigated the grass/soil concentration ratios (CRs) of natural radionuclides (226Ra, 232Th, and 40K) in vetiver grass collected from three different regions of Thailand using an HPGe (high-purity germanium) gamma spectrometer. The sampled grass blades were divided into three equal parts to investigate the dependence of grass/soil CRs on the positions of grass blades. The results showed that 40K had the highest grass/soil CRs due to being an essential nutrient for most plants, with ranges of 1.12–7.92, in comparison with 226Ra and 232Th that had ranges of 0.32–1.48 and 0.20–1.31, respectively. In addition, the results indicated that the natural radionuclides investigated were uniformly transferred and accumulated throughout grass blades (p < 0.05), which implied the independence of CRs on the section of the leaf. Furthermore, it was found that the soil samples collected from the central region of Thailand had the highest activity concentrations in all radionuclides, mainly due to a primary composition of dark clay, leading to the highest contents of potassium and calcium (Total K and Total Ca, respectively), and the highest cation exchange capacity (CEC) that led to high concentrations of nutrients and radionuclides. Based on the results from this work, the vetiver grass presented potentials to be utilized as radionuclide absorbers in polluted soil and water due to its high CRs in comparison with other reported plants.

Hydroponic phytoremediation of paperboard mill wastewater by using vetiver (Chrysopogon zizanioides)

Floating hydroponic system (FHS) is a potential and cost-effective technique for wastewater treatment. Vetiver (Chrysopogon zizanioides) is a more efficacious material for phytoremediation due to its physiological and morphological properties. A pilot-scale investigation on phytoremediation potential of FHS was carried out using Vetiver grass (Chrysopogon zizanioides) along with aeration on treated (TE) and raw effluents (RE) from paper board mill industry. Among the treatments, the vetiver hydroponic system with aeration had the highest treatment efficiency in the remediation of treated paper board mill effluent. The outcomes of the study exhibited a significant reduction in electrical conductivity (39.39%), total soluble salts (81.19%), and total dissolved solids (56.19%). The decline in Biological oxygen demand (55.68%) and chemical oxygen demand (58.01%) was evident at the end of the study period. The decrease in nutrients like total nitrogen (59.24%), phosphorus (47.78%), potassium (54.64%) was attributed to the growth of the vetiver grass. The lead concentration was dropped by 65.63% to 0.09 mg L−1 under the regime's permissible limits, and cadmium concentration decreased by 64.29%. From these results, the hydroponically grown vetiver treatment system combined with aeration poses a promising future for developing a sustainable, cost-effective methodology for pollutant detoxification in paperboard mill wastewater.

Erosion and control options in the La Ciénega Microbasin in Malinaltepec, Guerrero, Mexico

Objective: To estimate the water erosion rate in the La Ciénega Microbasin inMalinaltepec, Guerrero, Mexico, and to evaluate control options.Design/methodology/approach: Potential erosion rate, actual erosion rate, anderosion control were estimated using the Universal Soil Loss Equation (USLE) underthree conservation practices: contour plowing, successive land terracing, and liveterracing with vetiver grass (Chrysopogon zizanioides (L.) Roberty).Results: High erosion potential is shown by 99.18% of the microbasin surface area.Due to vegetation, only 41% of the surface area demonstrates high or very high actualerosion, 53.6% light, and 4.6% moderate. A living barrier using vetiver is the onlyconservation practice, of those assessed, that effectively diminished erosion.Study limitations/implications: The lack of accurate data on rain-induced erosion wasa limiting factor in this study. Findings/conclusions: The La Ciénega Microbasin has a high risk of erosion andcurrent erosion is a problem on agricultural land. The most effective option is livingbarriers using vetiver.

Removal of Antibiotics and Nutrients by Vetiver Grass (Chrysopogon zizanioides) from a Plug Flow Reactor Based Constructed Wetland Model.

Overuse of antibiotics has resulted in widespread contamination of the environment and triggered antibiotic resistance in pathogenic bacteria. Conventional wastewater treatment plants (WWTPs) are not equipped to remove antibiotics. Effluents from WWTPs are usually the primary source of antibiotics in aquatic environments. There is an urgent need for cost-effective, environment-friendly technologies to address this issue. Along with antibiotics, nutrients (nitrogen and phosphorus) are also present in conventional WWTP effluents at high concentrations, causing environmental problems like eutrophication. In this study, we tested vetiver grass in a plug flow reactor-based constructed wetland model in a greenhouse setup for removing antibiotics ciprofloxacin (CIP) and tetracycline (TTC), and nutrients, N and P, from secondary wastewater effluent. The constructed wetland was designed based on a previous batch reaction kinetics study and reached a steady-state in 7 days. The measured concentrations of antibiotics were generally consistent with the modeling predictions using first-order reaction kinetics. Vetiver grass significantly (p < 0.05) removed 93% and 97% of CIP and TTC (initial concentrations of 10 mg/L), simultaneously with 93% and 84% nitrogen and phosphorus, respectively. Results show that using vetiver grass in constructed wetlands could be a viable green technology for the removal of antibiotics and nutrients from wastewater.

The role of Charakokta Varnya Mahakashaya for maintenance of skin complexion (Varnya Vriddhi ) – a literature review

Beauty is a subject of socio-medical importance. Cosmetics are used in a wide range throughout the world which leads to many hazardous effects due to chemicals contain. The aim of this study is to evaluate the use and the mode of action of herbs mentioned in Varnya Mahakashaya of Acharya Charak for maintenance of skin complexion i.e. for Varna Vriddhi of Twaka or Rupaprasadan. According to Ayurveda Skin is divided in 7 different layers – Avabhasini, Lohita, Shweta, Tamra, Vedini, Rohini, Mamsadhara. These layers are responsible for various characters of skin like complexion, and also the Bhrajak Pitta which is closely associated with skin is responsible for skin complexion. The Charak Samhita is great text in Ayurveda stated a group of 10 medicinal plants known as Varnya Mahakashaya that can be used to obtain glowing skin. It includes- Shweta Chandan ( Sntalum albam ), Nagakeshar (Mesuva ferra), Padmak (Prnus cerasoides) , Ushira (Vetiveria zizanioides), Madhuka (Glycerrhiza glabra), Manjishtha (Rubia cordifolia), Sariva (Hemidesmus indicus), Payasya (Ipomoea digitata), Sita (white variety of Cynodon dactylon) and Lata (green veriety of Cynodon dactylon). These drugs can be used individually or in combination also internally and externally. All of them work under varnya, raktashodhak, pittashamak, kushthaghna guna. Most of them are prescribed for skin diseases, the pharmacological properties of these herbs indicate complexion promotion action.

Simultaneous removal of arsenic, fluoride, and manganese from synthetic wastewater by Vetiveria zizanioides.

Main aim of the present research is to explore the potential use of Vetiveria zizanioides L. for phytoremediation of arsenic, fluoride, and manganese simultaneously from synthetic wastewater in a batch scale floating platform unit. Half strength Hoagland's nutrient solution spiked with arsenic, fluoride, and manganese concentrations of 1, 20, and 10 mg/L, respectively has been used. The effects of pH and treatment time on simultaneous removal of arsenic, fluoride, and manganese have been performed. V. zizanioides has exhibited optimum growth at pH 8 and the removal of arsenic and fluoride is observed to be 59.6 and 38.1%, respectively. This plant has successfully removed all of the manganese (99.3%). The uptake of manganese is found to be faster than the arsenic and fluoride. The trend of arsenic, fluoride, and manganese accumulation in various parts of V. zizanioides is found as roots > stems > leaves. Result showed that the use of V. zizanioides would be appropriate to treat arsenic, fluoride, and manganese contaminated wastewater.

Structure-based assortment of herbal analogues against spike protein to restrict COVID-19 entry through hACE2 receptor: An in-silico approach

On-going global pandemic COVID-19 has spread all over the world and has led to more than 1.97 million deaths till date. Natural compounds may be useful to protecting health in this perilous condition. Mechanism of shuttle entry of SARS-COV-2 virus is by interaction with viral spike protein with human angiotensin-converting enzyme-2 (ACE-2) receptor. To explore potential natural therapeutics, 213 important phytochemi-cals of nine medicinal plants Aconitum heterophyllum, Cassia angustifolia, Cymbopogon flexuosus, Cymbopogon martinii, Nux vomica, Phyllanthus urinaria, Swertia chirayita, Justicia adhatoda, Vetiveria zizanioides were selected for in-silico molecular docking against the spike protein of SARS-COV-2 and compared with recently prescribed drug chloroquine, ramdesivir, lopinavir and hydroxychloroquine. Results revealed that rhamnocitrin of P. urinaria, 1,5-dihydroxy-3,8-dimethoxyxanthone of S. chirayita and laevojunenol of V. zizanioides potentially binds with the receptor binding site of SARS-COV-2 spike glycoprotein and more robustly destabilized the RBD-ACE-2 binding over chloroquine, ramdesivir, lopinavir and hydroxychloroquine. It was also found that laevojunenol, rhamnocitrin, and 1,5-dihydroxy-3,8-dimethoxyxanthone qualified the criteria for drug-likeness as per Lipinski rule. After attachment of the selected phytochemical with the spike protein the affinity of the later towards ACE-2 was minimized and the effect of 1,5-dihydroxy-3,8-dimethoxyxanthone and laevojunenol was superior. Hence, rhamnocitrin of P. urinaria, 1,5-dihydroxy-3,8-dimethoxyxanthone of S. chirayita and laevojunenol of V. zizanioides, are potential therapeutic molecules for SARS-COV-2, which upon binding with spike protein changes the affinity of the spike towards ACE-2 and therefore restrict the entry of the virus into a human cell. Subsequent clinical validation is needed to confirm these phytochemicals as drugs to combat COVID-19.

Characteristics of unsaturated soil slope covered with capillary barrier system and deep-rooted grass under different rainfall patterns

Rainfall-induced slope failures commonly occur in residual soil slopes. Slope failures are triggered by the reduction in soil strength. This is attributed to the decrease in soil suction due to rainwater infiltration. Slope covers like capillary barrier system and vegetative cover are effective methods that can be used to prevent rainfall-induced slope failures. The capillary barrier system is able to limit the rainwater infiltration, and vegetation can contribute to the increase in soil strength. Vetiver grass is widely planted in tropical and subtropical areas of the world for soil and water conservation. This study investigates the characteristics of unsaturated soil slope covered with capillary barrier system and Vetiver grass in comparison with the original slope through numerical analyses and field measurements. The analyses were carried out under the advanced, normal, and delayed rainfall patterns. The results of the analyses indicated that the capillary barrier system played a more significant role than the Vetiver grass in maintaining slope stability, although both the capillary barrier system and Vetiver grass contributed to the slope stability. In addition, both numerical analyses and field measurements showed that under the delayed and normal rainfall patterns, when antecedent rainwater infiltration could increase the soil moisture, the capillary barrier system performed much better compared to Vetiver grass in maintaining soil matric suctions and slope stability.

Response on Growth and Production of Vetiver (Vetiveria zizanioides L.) on Gibberelin Under Salinity Stress Conditions

Vetiver (Vetiveria zizanioides L.) plants are able to protect the soil from erosion and are tolerant of salinity stress. However, at high salinity levels, vetiver plants show a decreasing growth. The application of gibberelin is expected to increase the growth of vetiver in salinity stress conditions. The purpose of this study was to evaluate the growth responsiveness and production of vetiver the application of gibberelin under salinity stress conditions. This research was conducted in the green house of the Faculty of Agiculture, Universitas Sumatera Utara, Medan. This study used a non-factorial completely randomized design, namely the salinity stress (S) which consisted of 3 levels, each treatment was repeated 3 times, so that 9 treatment combinations were obtained: the concentration of gibberelin was 0 (no treatment), 50 ppm and 100 ppm.

GC-MS analysis and in silico activity prediction of phytocompounds in the roots of Chrysopogon zizanioides (L.) Roberty

Chrysopogon zizanioides (L.) Roberty (Poaceae) commonly known as Ramachamis an aromatic, vigorous growing perennial grass with medicinal properties. The plant is tolerant to extreme soil and climatic conditions and is known for its cooling properties. Roots of the plant are widely used as body scrubber and is suggested for skin diseases in Ayurveda. The present work aims to identify the components in the crude methanolic root extract of C. zizanioides using GC-MS and also to predict the pharmacokinetic behaviour of selected compounds in silico using Swiss ADME online server . 41 compounds were identified of which sesquiterpenes formed the major group. Sesquiterpene Vetivenic acid was the compound with a maximum peak area of 38.9%. Components identified is reported to possess a range of biological activities like anti oxidant, antibacterial, anti cancer, anti inflammatory, anti ulcer, analgesic and insecticidal activities. Compounds with higher peak area like Vetivenic acid, beta vatirenene, beta.-Cedren-9-.alpha.-ol, D Viridiflorol, Gamma muurolene, (Z,E)-alpha-farnesene, Nootkatone, Aromadendrene oxide-(2), 7-Acetyl-2-hydroxy-2methyl-5isopropylbicyclo[4.3.0] nonane, Rosifoliol, 9,10-dehydro isolongifolene, Ylangenol, 4,7,10,13,16,19-Docosahexaenoic acid methyl ester, Carbonic acid, propargyl 2,2,2-tri chloroethyl ester, Oxacyclotetradeca-4,11-diyne, beta eudesmol and longifolene were evaluated in silico. All these compounds proved to obey Lipinski's rule-of-five and were water soluble. Vetivenic acid showed a good bioavailability score of 85% while the others showed 55%. None of the compounds were substrates to P glycoprotein. The values predicted may be used for preliminary evaluation of pharmacological properties of C. zizanioides and also as monographs for the development of potential semisynthetic or synthetic drugs.

Provide Student Knowledge About How Response On Growth Of Vetiver Seeds (Vetiveria zizanioides) In Saline Soil To Ascorbic Acid on Field Practice Learning of Plant Physiology

Vetiver root is a commodity that is tolerant enough to be planted in salty soils with a certain level of salinity. One approach to increase oxidative stress tolerance that will increase the enzyme substrate at the cellular level is ascorbic acid. The purpose of this study was to increase students' knowledge about how the response of vetiver seed (Vetiveria zizanioides) growth in salt soils to ascorbic acid through practical field learning in plant physiology courses. This research was conducted using a factorial randomized block design (RBD), the first factor was the salinity (S), namely S0: 0 dsm-1 and S1: 4 dsm-1. The second factor was ascorbic acid (A), namely A0: without treatment, A1: 50 ppm, A2: 100 ppm and A3: 150 ppm. There were 8 treatment combinations that were repeated 3 times resulting in 24 experimental units. The parameters observed were plant height, number of leaves and number of stomata. In this study, ascorbic acid had a significant effect on plant height, number of leaves and number of stomata. Saline soil had a significant effect on the number of stomata parameters. No interactions for all parameters were observed.