Veterinary Treatment Research Articles

Re-emergence of Rift Valley fever: an update and preventive measures.

Re-emergence of Rift Valley fever: an update and preventive measures.

Dislocated Hip in Cattle: A Rare Find from a Roman Period Settlement

In this paper I discuss the remains of a Roman Period (2nd–3rd century AD) cow discovered at the site of Baracs–Boldog-dűlő in Transdanubia, Hungary. The animal lived with a dislocated hip, a condition extremely rarely documented in archaeological assemblages; this is the first find of this kind in the region. The animal may have been a dairy cow kept alive for months or even years after the injury, even though it was probably unfit for physical work or calving. This find provides a glimpse into how such injuries were treated, as there is contemporary written evidence about applied veterinary treatments.

Perceptions of the rabbit as a low investment 'starter pet' lead to negative impacts on its welfare: Results of two Danish surveys.

Concerns over compromised companion rabbit (Oryctolagus cuniculus domesticus) welfare are widespread. The welfare problems have been linked to the perception of rabbits as low investment 'children's pets.' To test this hypothesis and investigate the current conditions for rabbits, data were gathered from two surveys in 2021: a nationally representative survey of Danish companion animal owners (Survey I) and a detailed social media-based survey of Danish rabbit owners (Survey II). Using logistic regression, three owner-related variables (whether a child/adult was responsible for care of the rabbit, owner-opinion on rabbits' suitability as 'starter pets' and willingness-to-pay [WTP] for veterinary treatment) were employed to investigate the effect of rabbit status on owner-provision of selected husbandry conditions. The 76 (Survey I) and 4,335 (Survey II) responses suggested that most rabbits are acquired for children and are solitarily housed, and that many are kept in cages of an unsuitable size and not checked daily. Owners who perceived rabbits as 'starter pets' and with lower WTP were more likely to house rabbits in restricted space and to not provide continuous gnawing opportunities, ad libitum hay or routine healthcare. A child fulfilling the role of the rabbit's main caretaker was also associated with inadequate housing type and fewer gnawing opportunities. Thus, many rabbits live in unsuitable conditions, and owners who perceive rabbits as low investment 'children's pets' are more likely to not provide recommended resources. Changing owners' perceptions of rabbits and promoting suitable husbandry through official education programmes and minimum requirements is important if there are to be improvements made to rabbit welfare.

Epidemiological, clinico-diagnostic and therapeutic aspects of bovine respiratory disease in Indian buffaloes

Bovine Respiratory Disease (BRD) is major health problem occurring worldwide in bovines, responsible for high morbidity and mortality in calves and heavy economic losses in terms of production losses in milch animals and cost of veterinary treatment. Hence the present study was planned to investigate epidemiological, clinico-diagnostic and therapeutic aspects of BRD in Indian buffaloes. A total of 805 buffaloes reported to TVCC, COVAS, Udgir during the study period were screened for Bovine respiratory disease. On the basis of history of varying degree of nasal discharge, coughing, dyspnoea and abnormal lung sounds, 37 buffaloes were found positive for BRD, indicating 4.60% overall prevalence. The haemoanalysis showed significant (P<0.01) leukocytosis associated with neutrophilia. The radiographic examination of thorax revealed variable degree of congestion and diffuse consolidation of lungs. The bacteria isolated from nasal swabs were Staphylococcus sp., Streptococcus sp., E. coli, Corynebacterium sp., Klebsiella sp., Manheimia haemolytica, Brevundimonas sp. and Pseudomonas sp. Antibiogram of pure culture organisms isolated from BRD affected buffaloes revealed highest sensitivity for gentamicin (83.33%), followed by enrofloxacin (58.33%), ceftiofur sodium (50.00%), moxifloxacin (33.33%). Thirty-one BRD affected buffaloes were randomly distributed in four groups and treated with gentamicin 4 mg/ kg, enrofloxacin 5 mg/kg, moxifloxacin 5 mg/kg and ceftiofur 1.6 mg/kg respectively. All treated buffaloes from different groups clinically cured within 5 to 15 days. Based on results of current therapeutic trial, gentamicin proved most effective and economic in treatment of BRD in buffaloes.

Pre-anaesthesia preparation in cats and dogs – recent evidence

Nurses and technicians practicing veterinary anaesthesia should be familiar with current literature on preanaesthesia preparation for cats and dogs. Reductions in morbidity and mortality in animals undergoing general anaesthesia can be achieved by using low-stress veterinary treatments to allow for thorough physical examinations, diagnostic testing, assessment of anaesthetic risks and intervention planning. This article describes morbidity and mortality in veterinary patients and highlights some recent evidence on the importance of anaesthetic checklists, pre-visit pharmaceutical options, low-stress handling, fasting protocols and associated therapeutics, and the value of pre-anaesthetic laboratory work.

The autonomy principle in companion veterinary medicine: A critique.

Following developments in human medical ethics, veterinary ethics has similarly shifted from a historic paternalistic approach, toward greater respect for autonomy. Veterinarians operate within a tripartite system where there is separation of doctor/patient dyad by animal owners. As such there are fundamental differences between veterinary and human medical sectors regarding application of the autonomy principle—specifically, to whom is autonomy afforded? This paper argues that the accepted transference of autonomy to owners constitutes a corruption of the principle. Privileges owners exercise over animal treatment decisions relate to their rights over property use, rather than application of self-rule over one's own person as described in bioethics literature. To highlight issues with the status quo, this paper outlines the negative consequences of “owner autonomy” on animal (patient) welfare, integrity of the veterinary profession's social contract and professional autonomy. A way forward is proposed that places greater emphasis on animal (patient) welfare being explicitly at the center of veterinary treatment decision-making via recognition that all such decisions are made by a proxy, and therefore more appropriate frameworks ought to be engaged, such as a best interests paradigm.

Intensive Adoption as a Management Strategy for Unowned, Urban Cats: A Case Study of 25 Years of Trap-Assess-Resolve (TAR) in Auckland, New Zealand.

Simple SummaryUnowned urban cats may suffer from poor welfare and cause problems, e.g., public health risks, nuisances, and urban wildlife predation. For 25 years, the Lonely Miaow (Inc.) charity in Auckland, New Zealand, has used intensive adoption to rehome unowned cats. By the end of 2019, LM volunteers had trapped 14,611 unowned cats, which were adopted wherever possible (64.2%), euthanized if unsocialised or in grave ill-health (22.2%), or (infrequently) neutered and returned to the site (5.7%). The remaining 7.9% had other outcomes, such as being transferred to other shelters. Adoption rates increased over time, exceeding 80.0% in 2018 and 2019. The cost of processing each cat from capture to adoption rose from NZD 58 in 1999 to NZD 234 in 2017. Approximately 80% of colonies (sites where cats were trapped) were around residential areas. Most cats were young and very few were over 5 years old. Around one in five cats needed veterinary treatment, with respiratory infections and injury common. Adopting cats and removing them from the streets improves their welfare, essentially benefitting the community and the cats. The effectiveness of adoption strategies would be enhanced by fewer abandonments of owned cats and kittens, fitting within integrated strategies for the control of unowned cats involving community education.Globally, unowned urban cats are a major concern because they may suffer from poor welfare and cause problems, including public health risks, nuisances, and urban wildlife predation. While management options are often presented as a choice between culling or trap–neuter–return (TNR), for 25 years, the Lonely Miaow (Inc.) charity in Auckland, New Zealand (hereafter LM), has used a third strategy—intensive adoption or trap–assess–resolve (TAR). As of 2019, of 14,611 unowned cats trapped, 64.2% were adopted, 22.2% were euthanized if unsocialised or in grave ill-health, 5.7% were neutered and returned to the site, and 7.9% had other outcomes, such as being transferred to other shelters. Adoption rates increased over this time, exceeding 80.0% in 2018 and 2019. The cost of processing each cat from capture to adoption rose from NZD 58 in 1999 to NZD 234 by 2017. Approximately 80% of colonies (sites where cats were trapped) were around residential areas. Approximately 22% of cats required veterinary treatment after capture; common ailments included respiratory infections, ringworm, dental problems, and trauma. Consistently, 52% of cats were young kittens (<10 weeks old), c. 80% of cats were <1 year old, and only c. 2% were estimated to be >5 years old. TAR avoids euthanasia where possible. Its effectiveness would be enhanced by fewer abandonments of owned cats and kittens, fitting within integrated strategies for the control of unowned cats involving community education. Cat adoptions improve the welfare of cats and, with appropriate husbandry, should alleviate concerns about nuisances, public health, and attacks on wildlife or the cats themselves, essentially benefitting the community and the cats. This case study is relevant to other cities around the world that are seeking to manage unowned cats.

Genome-wide scan for selective sweeps identifies novel loci associated with resistance to mastitis in German Holstein cattle.

Domestication and selection significantly changed phenotypic and behavioural traits in modern domestic animals. In this study, to identify the genomic regions associated with mastitis, genomic data of German Holstein dairy cattle were analysed. The samples were genotyped using the Bovine 50 K SNP chip. For each defined healthy and sick group, 133 samples from 13,276 genotyped dairy cows were selected based on mastitis random residual effects. Grouping was done to infer selection signatures based on XP-EHH statistic. The results revealed that for the top 0.01 percentile of the obtained XP-EHH values, five genomic regions on chromosomes 8, 11, 12, 14 and 26 of the control group, and four regions on chromosomes 3, 4 (two regions) and 22 of the case group, have been under selection. Also, consideration of the top 0.1 percentile of the XP-EHH values, clarified 21 and 15 selective sweeps in the control and case group, respectively. This study identified some genomic regions containing potential candidate genes associated with resistance and susceptibility to mastitis, immune system and inflammation, milk traits, udder morphology and different types of cancers. In addition, these regions overlap with some quantitative trait loci linked to clinical mastitis, immunoglobulin levels, somatic cell score, udder traits, milk fat and protein, milk yield, milking speed and veterinary treatments. It is noteworthy that we found two regions in the healthy group (on chromosomes 12 and 14) with strong signals, which were not described previously. It is likely that future research could link these identified genomic regions to mastitis. The results of the current study contribute to the identification of causal mutations, genomic regions and genes affecting mastitis incidence in dairy cows.

Real-World Data on Antibiotic Group Treatment in European Livestock: Drivers, Conditions, and Alternatives.

Major efforts have been made by veterinary professionals to reduce the need for antibiotic use in animals. An online survey launched by the Federation of Veterinarians of Europe (FVE) aimed to gather responses from practicing veterinarians with field experience in metaphylactic livestock group treatment. Only 17% of all veterinarians (n = 183/1087, all species-specific responses merged) applied metaphylactic group treatments to 75% or more of all their treatments. Significantly less metaphylactic group treatments were reported in mixed practices (p = 0.002) and practices specialized in cattle (p < 0.001) as well as small (p = 0.007) and very small practices (p = 0.009). Gram-negative bacteria, mostly composed of Enterobacteriaceae and Pasteurellaceae, were considered by 75.3% (n = 967/1385) as the most devastating bacterial pathogens. Respondents alleged morbidity (20.1%, n = 201/998) and mortality (42.2%, n = 421/998) as major consequences for animal health and welfare if metaphylaxis would be banned. Responding veterinarians pointed towards vaccinations; improved biosecurity, including hygiene measures; and improved herd health management as the three most effective alternative measures to prevent metaphylactic treatment. However, more research is needed on how to implement appropriate alternatives in a holistic hurdle approach. Active support on a national level will be necessary for the development and application of targeted veterinary treatment guidelines for practitioners, which promote the understanding of drivers and include initiation criteria for metaphylactic group treatments in livestock.

Traditional Chinese Veterinary Medicine to Treat Oral Cancer in a Labrador Retriever

A 5-year-old male Labrador retriever was presented for traditional Chinese veterinary medicine (TCVM) evaluation and treatment after a diagnosis of oral squamous cell carcinoma of the right rostral mandible. The large tumor (5x4x1 cm) displaced the right mandibular canine tooth, lower incisors and extended ventrally under the tongue. A guarded prognosis had been given by an oncologist with an estimate of 3-6 months survival with palliative care. With the dog’s quality of life the most important consideration, the owner elected to try an integrative medicine approach combining piroxicam, tramadol and TCVM. The TCVM treatment strategies focused on tonifying Spleen Qi, and moving Qi and Blood. Chinese herbal therapy and food therapy were instituted as the central components of the treatment plan. A specifically-designed home-cooked diet was started to tonify Qi, resolve Stagnation and transform Phlegm. Emphasis was placed on food therapy as good nutrition has been shown to increase survival times in humans and animals with cancer. Over a 4-6 month treatment period, clinical signs steadily improved with resolution of the visible oral mass and the pain associated with it. The dog maintained an excellent quality of life for 56 months after the initial diagnosis. This case highlights an excellent clinical outcome for a dog with poor prognosis for long-term survival through an integrative approach using TCVM therapies.

Seaweed Phenolics as Natural Antioxidants, Aquafeed Additives, Veterinary Treatments and Cross-Linkers for Microencapsulation.

Driven by consumer demand and government policies, synthetic additives in aquafeed require substitution with sustainable and natural alternatives. Seaweeds have been shown to be a sustainable marine source of novel bioactive phenolic compounds that can be used in food, animal and aqua feeds, or microencapsulation applications. For example, phlorotannins are a structurally unique polymeric phenolic group exclusively found in brown seaweed that act through multiple antioxidant mechanisms. Seaweed phenolics show high affinities for binding proteins via covalent and non-covalent bonds and can have specific bioactivities due to their structures and associated physicochemical properties. Their ability to act as protein cross-linkers means they can be used to enhance the rheological and mechanical properties of food-grade delivery systems, such as microencapsulation, which is a new area of investigation illustrating the versatility of seaweed phenolics. Here we review how seaweed phenolics can be used in a range of applications, with reference to their bioactivity and structural properties.

Chemical and Pharmacological Properties of Decoquinate: A Review of Its Pharmaceutical Potential and Future Perspectives.

Decoquinate (DQ) is an antimicrobial agent commonly used as a feed additive for birds for human consumption. Its use as an additive is well established, but DQ has the potential for therapy as an antimicrobial drug for veterinary treatment and its optimized derivatives and/or formulations, mainly nanoformulations, have antimicrobial activity against pathogens that infect humans. However, DQ has a high partition coefficient and low solubility in aqueous fluids, and these biopharmaceutical properties have limited its use in humans. In this review, we highlight the antimicrobial activity and pharmacokinetic properties of DQ and highlight the solutions currently under investigation to overcome these drawbacks. A literature search was conducted focusing on the use of decoquinate against various infectious diseases in humans and animals. The search was conducted in several databases, including scientific and patent databases. Pharmaceutical nanotechnology and medicinal chemistry are the tools of choice to achieve human applications, and most of these applications have been able to improve the biopharmaceutical properties and pharmacokinetic profile of DQ. Based on the results presented here, DQ prototypes could be tested in clinical trials for human application in the coming years.

Relationship among Sex, Skin Color, and Production Parameters of Broiler in Pectoral Myopathies.

Simple SummaryGenetic selection in poultry production aims to obtain more efficient and fast-growing broilers. Breast is an economically interesting poultry cut; thus, its increased development, known as hypertrophy, is a selection criterion that has been adopted by breeders. However, genetic drift is increasing breast myopathies, alterations in the normal arrangement of the muscle fibers that compose the muscle, among broilers. The altered breast meat is fit for human consumption, and it does not represent a public health issue; nevertheless, it causes important commercial losses. Among all of the possible factors that may contribute to breast myopathies, the relationship between sex, skin color, live weight, conversion rate, and feed particle size and two types of alterations (wooden breast and spaghetti meat) was evaluated. It was found that such factors significantly affected the appearance of these myopathies in most cases, influencing muscle alterations in different and even opposite ways. Although the incidence of myopathies in the breast was low in the broilers studied, it is necessary to adapt the production conditions to offer a product of the highest quality.Breast anomalies in broilers, especially wooden breast (WB) and spaghetti meat (SM), cause high economic losses to the poultry meat sector. In order to identify the parameters that have a causal effect and to reduce the incidence of these myopathies, 141,792 broilers were analyzed in a total of 1477 batches using a visual grading system. The relationship among productive parameters such as the feed conversion ratio, live weight, growth rate, and mortality, was evaluated. Effects due to skin color (white vs. yellow), broiler sex (male, female, and mixed groups), feed presentation (grain vs. mash), and veterinary treatments (treated vs. untreated) were also included in the statistical study. Live weight was observed to have a significant effect (p < 0.001) on WB incidence, which increased by 1.11 for each 100 g of weight. Weight did not significantly affect the incidence of SM. Males had a higher incidence of WB and a lower incidence of SM than females. The incidence of both myopathies varied between samples that turned out to be significantly affected by some of the variables considered in the model, such as grain feeding and the feed conversion ratio. Controlling these factors in the broiler production could help to reduce the incidence of WB and SM.

Ethnozoological Study of Traditional Medicinal Animal Parts and Products Used among Indigenous People of Assosa District, Benishangul-Gumuz, Western Ethiopia

Traditional medicine is a global practice and depends on locally available natural resources and indigenous knowledge. Animals and their products have been used in the preparation of traditional remedies in various cultures since time immemorial. This study aimed to identify and document traditional medicinal sources from animals and associated indigenous knowledge in Assosa Districts, Benshangul Gumuz region, western Ethiopia, from September 2019 to July 2020 to ensure sustainable utilization of natural resources and biodiversity. A cross-sectional survey design was used to collect ethnozoological information with regard to animals used for medicinal purposes, parts used, ingredients added, ailments treated, method of preparation, mode of administration, dosage and duration of treatment, and the mechanisms of knowledge transfer. A total of 38 animal species were used as medicine to treat over 35 different kinds of human diseases including anatomical, physiological, psychological, and spiritual ailments and veterinary treatment. Over 15 animal species were found to score fidelity level (FL) more than 50%, of which the skin of Crocuta crocuta used for the treatment of evil eye had the highest FL (n = 35, 92.1%), followed by the blood of Sus scrofa domestica for treatment of stomach illness (n = 32, FL∼84.2%), the milk of Equus asinus to treat cough and eye disease (n = 28, Fl∼73.7%). The study area harbors diverse medicinal animals that represent key medical alternatives for local communities. The documentation of this indigenous knowledge of animal-derived medicine and the practice helps in developing strategies for conservations of biological diversities.

Melanocortin‐1 receptor influence in equine opioid sensitivity

SummaryIndividual variation in opioid sensitivity can have a profound impact on the safety and efficacy of equine veterinary treatments, with the ability to adequately manage equine pain in a clinical setting currently limited. This review aims to explore the overlap between biological mechanisms associated with opioid metabolism and those mechanisms associated with coat colour in horses as has been documented in humans, with particular focus on the melanocortin‐1 receptor (MC1R) gene. In the future, the use of the MC1R coat colour genotype could help to indicate variable opioid sensitivities thereby greatly improving the use of opioids in clinical settings. The MC1R gene has a well‐established role in melanogenesis and pigment switching, but involvement in the pain‐modulating periaqueductal grey (PAG) descending pathway and in immune responses, both of which contain opioid receptors, has also been suggested in humans. However, this relationship between opioid metabolism and the connection to the three known MC1R variants (EE, Ee and Eea) in horses is yet to be explored.

Diagnostic and therapeutic strategy for Clostridium perfringens infection in postpartum dairy cows: a report of 14 cases

ABSTRACT Clostridium perfringens type A infections are economically devastating to dairy farms. In spite of low morbidity, mortality is close to 100%. Although this disease has frequently been reported in calves, it is rarely seen in adult cattle, especially postpartum dairy cows. Sudden onset of symptoms, rapidly followed by death, was successively recorded in 14 postpartum dairy cows. Veterinary treatment was ineffective. The 14 cows fell ill after parturition, either within 24 h (fast invasion) or within 2–3 days (slow invasion). All the cows died shortly after symptom onset. Comprehensive analysis of epidemiology, clinical manifestation, autopsy results, and laboratory tests confirmed the 14 cases as most acute and acute C. perfringens type A infections. After the enactment of appropriate preventive and treatment measures, in conjunction with improved feeding management, no further symptoms of infection were observed in the postpartum cows. Our results demonstrated that autopsies and laboratory tests are required for the definitive diagnosis of this disease and highlighted importance of feeding management during the early perinatal period to prevent postpartum diseases in cows. This work also provides a reference for clinical veterinary diagnosis and the treatment of this disease, so as to reduce the economic losses of dairy farms.

Antibacterial and anti-virulence effects of furazolidone on Trueperella pyogenes and Pseudomonas aeruginosa

BackgroundTrueperella pyogenes and Pseudomonas aeruginosa are two important bacterial pathogens closely relating to the occurrence and development of forest musk deer respiratory purulent disease. Although T. pyogenes is the causative agent of the disease, the subsequently invaded P. aeruginosa will predominate the infection by producing a substantial amount of quorum-sensing (QS)-controlled virulence factors, and co-infection of them usually creates serious difficulties for veterinary treatment. In order to find a potential compound that targets both T. pyogenes and P. aeruginosa, the antibacterial and anti-virulence capacities of 55 compounds, which have similar core structure to the signal molecules of P. aeruginosa QS system, were tested in this study by performing a series of in vitro screening experiments.ResultsWe identified that furazolidone could significantly reduce the cell densities of T. pyogenes in mono-culture or in the co-culture with P. aeruginosa. Although the growth of P. aeruginosa could also be moderately inhibited by furazolidone, the results of phenotypic identification and transcriptomic analysis further revealed that sub-inhibitory furazolidone had remarkable inhibitory effect on the biofilm production, motility, and QS system of P. aeruginosa. Moreover, furazolidone could efficiently protect Caenorhabditis elegans models from P. aeruginosa infection under both fast-killing and slow-killing conditions.ConclusionsThis study reports the antibacterial and anti-virulence abilities of furazolidone on T. pyogenes and P. aeruginosa, and provides a promising strategy and molecular basis for the development of novel anti-infectious drugs to dealing with forest musk deer purulent disease, or other diseases caused by T. pyogenes and P. aeruginosa co-infection.

A One Medicine Mission for an Effective Rabies Therapy.

Despite the disease's long history, little progress has been made toward a treatment for rabies. The prognosis for patient recovery remains dire. For any prospect of survival, patients require aggressive critical care, which physicians in rabies endemic areas may be reluctant or unable to provide given the cost, clinical expertise required, and uncertain outcome. Systematic clinical research into combination therapies is further hampered by sporadic occurrence of cases. In this Perspective, we examine the case for a One Medicine approach to accelerate development of an effective therapy for rabies through the veterinary care and investigational treatment of naturally infected dogs in appropriate circumstances. We review the pathogenesis of rabies virus in humans and dogs, including recent advances in our understanding of the molecular basis for the severe neurological dysfunction. We propose that four categories of disease process need to be managed in patients: viral propagation, neuronal degeneration, inflammation and systemic compromise. Compassionate critical care and investigational treatment of naturally infected dogs receiving supportive therapy that mimics the human clinical scenario could increase opportunities to study combination therapies that address these processes, and to identify biomarkers for prognosis and therapeutic response. We discuss the safety and ethics of this approach, and introduce the Canine Rabies Treatment Initiative, a non-profit organization with the mission to apply a One Medicine approach to the investigation of diagnostic, prognostic, and therapeutic options for rabies in naturally infected dogs, to accelerate transformation of rabies into a treatable disease for all patients.

Prevalence and molecular characteristics of extended spectrum β-lactamase producing Escherichia coli isolated from retail chicken meat sold at the modern and traditional markets in Jakarta, Indonesia

The use of antibiotics in veterinary and human treatment can cause the development of antibiotic resistance in bacteria for β-lactam antibiotics. Worldwide, this resistance has become a growing concern in public health. Limited data are currently available regarding Extended Spectrum β-Lactamase (ESBL) Escherichia coli in Indonesia. The current study determined the prevalence and characteristics of ESBL genes of E. coli in retail chicken meat and humans in Indonesia. Two hundred eighty retail chicken meat were randomly collected from various modern and traditional markets in Jakarta (70 retail sourced chicken meat from modern markets and 210 from traditional markets). The prevalence of E. coli from the chicken meat sold at traditional markets was 97.14%, which was significantly higher than those of the modern markets with 78.57 % (P &lt; 0.05). The prevalence of ESBL-producing E. coli isolated from chicken meat sold at traditional market was 40.47% and the modern market was 35.71 % and the prevalence of ESBL-producing E. coli isolated from chicken meat was 38.09 %, which is significantly higher than those of the clinical sample (with average 5.57 %). The most predominant gene is blaTEM in 54.54 % as a single gene or mixed with other genes followed by blaCTX-M in 44.31 % and blaSHV gene was only found in three isolates in 1.13 %. This study found that isolates from both the broiler chicken meat and clinical samples were having the same molecular characteristics. It is speculated that there is a relationship between them. However, this needs to be substantiated further.

Revenues and costs of dairy cows with different voluntary waiting periods based on data of a randomized control trial

Based on modeling studies, a 1-yr calving interval for dairy cows is generally considered optimal from an economic point of view. Recently some dairy farmers are deliberately extending the voluntary waiting period for insemination (VWP) to extend the calving interval. Reasons to extend the VWP are to reduce the frequency of transitions such as dry-off and calving to improve health, to reduce labor associated with these transitions, and to reduce the number of surplus calves. This study aimed to evaluate yearly revenues, yearly costs, and yearly net partial cash flow (NPCF) for individual cows with a VWP of 50, 125, or 200 d based on data from a randomized control trial. The NPCF included revenues and costs for milk yield, calves born, inseminations, concentrate supply, partial mixed ration (PMR) supply, veterinary treatments, discarded milk due to veterinary treatments, culling, and labor (for milking, calving cows, inseminations, and veterinary treatments). Holstein-Friesian dairy cows (n = 153) within one herd were blocked for parity, calving season, and expected (primiparous cows) or previous (multiparous cows) 305-d milk yield. Cows were randomly assigned within the blocks to 1 of 3 VWP (VWP50, VWP125, or VWP200) in wk 6 after calving, and monitored from wk 6 after calving until wk 6 after the next calving or until culling. Revenues and costs were calculated per individual cow and expressed per cow per year. Revenues from milk and costs for PMR and concentrate contributed most to the yearly NPCF. Total yearly revenues were greater in VWP50 compared with VWP200 (€3,169 vs. €2,832), mainly because of €334 greater milk revenues. Total yearly costs were also greater in VWP50 compared with VWP200 (€1,964 vs. €1,729), mainly because of €102 greater concentrate costs. The VWP was not significantly associated with the NPCF per cow per year. A change in milk, feed, or calf price, or a change in labor costs for calving cows or for inseminations had a greater effect on the yearly NPCF of cows in VWP50 compared with cows in VWP200. To investigate variation in NPCF, cows were grouped for yearly NPCF and categorized into 3 economic classes (EC): EC1 (<€1,100/yr), EC2 (€1,100-€1,400/yr), and EC3 (>€1,400/yr). Cows in EC3 had greatest lactation production per day in the experiment (i.e., kg of milk, protein, fat, lactose), and lowest number of veterinary treatments during the experiment.