Veterinary Inspection Research Articles

Prevalence of Some Zoonotic Parasitic Affections in Sheep Carcasses in a Local Abattoir: In Cairo, Egypt

Meat-borne zoonotic parasites raised from consumption of undercooked and/or crossly contaminated meats can infect human by direct and/or indirect ways causing many mild to serious diseases; so in the present study, some meat-borne transmissible parasites were investigated in 5239 freshly dressed sheep carcasses in local Egyptian abattoir located at Cairo governorate along the period of 2017-2018. Results of Post- Mortem inspection revealed the detection of Fasciola, Cysticercus and Hydatid cyst in 3.47, 1.06 and 1.83% of the total examined carcasses with total economic losses of 9306.78 L.E, 2892 L.E and 4380 LE, respectively. It is worthily noted that fascioliasis recorded the highest incidence of infection, followed by hydatidosis and cysticercosis in the examined carcasses, respectively. Cysticercus ovis and C. tenuicollis were detected in 32.14% and 67.85% with total economic losses of 714 L.E and 2178 L.E because of infected heart and liver condemnation during 2017 and 2018, respectively. Furthermore, hydatid cyst was detected in 0.47% and 1.35% of the examined lung and liver samples with total economic losses of 1188 L.E and 3192 L.E because of infected lung and liver condemnation during 2017 and 2018, respectively. Referring to the obtained results, it was obvious that parasitic infection of sheep meat and offal constrains a lot of economic losses, threatens the meat production industry and throws lights over the health importance of veterinary inspection great role in protecting human-being from be infested with zoonotic meat-borne parasites. So, magnification and great support should be given to training veterinary inspectors in slaughter houses in Egypt.

Prevalence of Some Foodborne Parasitic Affection in Slaughtered Animals in Loacal Egyptian Abottoir

Fascioliasis, cysticercosis and hydatidosis were aimed to be investigated in some slaughtered animals (cattle, buffalo and camel) in local Egyptian abattoir located in Cairo governorate. 10317, 763 and 290 cattle, buffalo and camel carcasses, respectively were examined in the period of 2017-2018. Results of PM inspection along the investigation period revealed detection of Fasciola in 0.58 and 1.4% of the examined cattle and buffalo carcasses, respectively, while was not detected in camel carcasses. Also, cysticercosis was detected in 0.47 and 0.69% of the examined cattle and camel carcasses, respectively; while was not detected in buffalo carcasses. In addition, hydatidosis was detected in lung and liver of 0.038 and 0.096% of cattle carcasses; 3.4 and 1.03% of camel carcasses, respectively, while was not detected in the examined buffalo samples. Annual values of financial losses because of the condemned affected parts summed total of 11712.5, 32940.0 and 2410 LE due to fascioliasis, cysticercosis and hydatidosis, respectively along the investigation period (2017 and 2018). Referring to the obtained results, records of 2017 appeared to be more infection prevalent than 2018 with more financial losses, moreover, fascioliasis was the most prominent affection in the present study. In addition, the critical veterinary inspection has a great role in protecting human-being to be infected with zoonotic meat-borne parasites. So, magnification and great support should be given to training veterinary inspectors in slaughter houses in Egypt.

Phytogenic Ingredients from Hops and Organic Acids Improve Selected Indices of Welfare, Health Status Markers, and Bacteria Composition in the Caeca of Broiler Chickens.

Simple SummaryThe selection for the rapid growth rate in broiler chickens that has been carried out over the years has negatively influenced their health and welfare status. In recent years, a number of reports have been delivered on the use of additives that improve broilers’ intestinal peristalsis and production results. The authors of this paper have proved that applying a mixture with 50% hops (manifesting strong antioxidant, antibacterial, and antifungal properties) may bring benefits to the quantity and quality of the final product. This may refer to the production performance, flock health status, and welfare of birds. The thematic scope of this research is currently of significant importance, as veterinary inspections pay particular attention to the quality of litter and the welfare of birds, and this motivates producers to improve breeding conditions, which will contribute to better production systems.The objective of this study was to determine the influence of phytogenic product-supplemented, organic acid-supplemented, and prebiotic-supplemented diets on the production results, antioxidative status, and selected welfare indices in broiler chickens. A total of 1155 one-day old male Ross 308 broilers were randomly assigned to one of three treatment groups: Group C, no additives; Group A, supplemented with phytogenic supplement (50% hop); and Group P, supplemented with 65% organic acids and their salts, and 30% prebiotic complex. Health condition and production results were monitored during the entire experiment. After 42 days, 10 birds from each dietary treatment group were selected for blood sampling and slaughter analysis. The results obtained revealed that over the whole feeding period, none of the investigated additives significantly affected broiler performance indices. However, feeding the birds treatment-A increased the relative abundance of Bifidobacterium in caecal digesta compared to the other treatments, whereas feeding treatment-P increased the relative abundance of Lactobacillus compared to the control treatment. Overall, treatment-A was more effective at increasing relative abundance of Clostridia in birds at 42 days of age than treatment-P. Finally, there were no changes in blood levels of antioxidant indices or liver function indicators.

Factors associated with completion of Fédération Équestre Internationale endurance rides (2012–2019): Modelling success to promote welfare-oriented decisions in the equestrian sport of endurance

Endurance riding is a popular equestrian sport organised at the international level by the Fédération Équestre Internationale (FEI). The sport involves prolonged exercise at speed over significant distances, which puts a substantial load on equine athletes’ musculoskeletal systems and metabolism and contributes to the risk of injuries and other veterinary problems. The FEI employs a system of in-ride veterinary inspections aimed at early detection of such problems and elimination of horses unfit to continue in the ride. To date, risk factors for elimination due to irregular gait or metabolic problems have been the primary focus of scientific enquiry. The present study is the first large-scale multivariable study to report factors associated with the positive outcome of endurance rides – their successful completion. This retrospective observational cohort study used data from the FEI’s Global Endurance Database. The study cohort included 74,629 starts made by 21,346 unique horses in one-day international-level competitions held worldwide between July 2012 and December 2019. Forty-one potential factors identified based on previous studies and a priori hypotheses were considered in the analysis. Univariable and multivariable logistic regression analyses were performed to investigate associations between ride completion and predictor variables and their interactions. The study identified 19 factors and four interactions associated with an increased or decreased likelihood of successful ride completion. High competition frequency (fewer days over mandatory out of competition period; Wald χ2 71.35, P-value <0.0001), fast riding speed (236.57, <0.0001), upward changes in competition level and riding speed between two successive rides (103.59, <0.0001 and 87.60, <0.0001) were associated with a decrease in the likelihood of ride completion. These factors can be effectively regulated by appropriate rules and considered by athletes when planning competition schedules, preparing ride strategies, or indeed during the ride itself. Other identified factors, including horse and rider age, sex, completions and eliminations history, contribute to an estimate of the likelihood that a particular combination completes a ride and thus can aid veterinarians at veterinary inspections as additional inputs supporting their decision-making when assessing horses’ fitness to continue in the competition. The study’s main limitation is an absence of data on national-level rides. Such data and data on training and veterinary histories remain difficult to access and were unavailable for the analysis. The present study’s results demonstrate that shifting the focus to modelling the positive outcome is a valuable approach offering evidence-based recommendations for good horsemanship, better-informed veterinary inspections, and welfare-oriented rules.

Biosensors for animal health and meat safety monitoring: farm-to-slaughterhouse continuum

The meat supply chain needs to be managed for sufficient levels of consumer protection. Healthy animals are an essential precondition for a safe food supply, since zoonotic diseases, including meatborne pathogens, are a major threat to public health. Information about the livestock’s general health, animal welfare and prevalence of major meatborne hazards such as Salmonella, Campylobacter, STEC and Listeria monocytogenes is of utmost importance for effective biosecurity control on farm. Early detection of these hazards in faecal samples, monitoring blood levels of metabolites relevant for animal welfare (hormones) and animal health (acute phase proteins) can provide high-level control in the animal farming industry. Multiplex biosensors for pathogens and metabolites in the farm-to-slaughterhouse continuum constitute a practical and cost-efficient tool for early detection of signs related to meat safety. Point-of-care multiplex biosensors are an advantage versus commonly used methods ELISA and RT-PCR, since they provide possibilities for early detection and do not require expensive equipment, trained personnel or significant time for sample transfer and analyses. Biosensors can improve meat inspection and meat safety controls, and can serve as a primary tool for monitoring food safety parameters and contribute to the modernization of veterinary inspection and risk-based meat safety assurance system.

ASSESSMENT OF FASCIOLA GIGANTICA INFECTION IN THE LIVER OF CATTLE SLAUGHTERED IN IKPOBA/OKHA AREA COUNCIL, EDO STATE, NIGERIA

This study investigated the prevalence and intensity of Fasciola gigantica infection in the liver of cattle slaughtered in three abattoirs located in Ikpoba/Okha Local Government Area of Edo State, Nigeria. It also estimated the economic implications of the condemned liver. A total of 17,325 cattle were examined in the three abattoirs over a period of 252 days (approximately 69 cattle/day) spanning over the wet and dry seasons; 1,683 cattle were infected with an overall prevalence of 9.71%. In Abattoir A, 11774 cattle were examined with 1069 (9.08%) prevalence. In Abattoirs B and C, 4352 and 1199 cattle, respectively, were examined and 563 (12.94%) and 51 (4.25%) were respectively infected. From the three abattoirs, 155 (0.89%) livers were considered unfit for human consumption due to F. gigantica infection. For the wet season, 1062 (10.89%) cattle livers were infected while 621 (8.20%) were infected during the dry season. The mean intensity of infection during the wet season was 37.80 and 30.42 during the dry season. The financial loss due to infected liver condemnation was at N803,160 (1,947.050USD) showing that liver condemnation arising from F. gigantica infection is a major cause of financial loss to cattle farmers and dealers. There is, therefore, need for adequate veterinary inspections for early detection and treatment of the disease. There should be more meat inspection, especially in private/individual abattoirs.

Severe Acute Respiratory Syndrome Coronavirus 2 in Farmed Mink (Neovison vison), Poland.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent of coronavirus disease and has been spreading worldwide since December 2019. The virus can infect different animal species under experimental conditions, and mink on fur farms in Europe and other areas are susceptible to SARS-CoV-2 infection. We investigated SARS-CoV-2 infection in 91 mink from a farm in northern Poland. Using reverse transcription PCR, antigen detection, and next-generation sequencing, we confirmed that 15 animals were positive for SARS-CoV-2. We verified this finding by sequencing full viral genomes and confirmed a virus variant that has sporadic mutations through the full genome sequence in the spike protein (G75V and C1247F). We were unable to find other SARS-CoV-2 sequences simultaneously containing these 2 mutations. Country-scale monitoring by veterinary inspection should be implemented to detect SARS-CoV-2 in other mink farms.

Serum biochemistry and haematology in wild and captive bearded seals (Erignathus barbatus) from Svalbard, Norway

BackgroundHealth assessment of seals in captivity include haematology and serum biochemistry measurements. Because such parameters differ between species, it is crucial to have species-specific reference values for the interpretation of clinical samples. Furthermore, differences in nutrition and environment, life cycles as well as seasonal/annual cycles and varying physiological conditions can potentially affect serum chemistry and haematology parameters. Blood samples from four captive adult bearded seals (initially caught as pups in Svalbard, Norway, now held at Polaria, an Arctic experience centre in Tromsø, Norway) collected over a 16-month period were analysed for haematology (n = 22) and serum chemistry (n = 25) parameters. Serum chemistry analyses were also conducted on blood samples from 74 wild bearded seals (1995–2007) collected from Svalbard, Norway.ResultsWe found higher activity of creatine kinase (CK) and higher concentrations of cortisol in the wild animals when compared to the captive seals, probably reflecting the physical restraint and concomitant stress induced during sampling. For the captive bearded seals, we did not find marked differences in haematology or serum chemistry parameters throughout the different seasons of sampling.ConclusionsThis study presents haematology and serum chemistry reference values for captive and wild bearded seals. Comparing physiological parameters for captive seals with wild seals indicated that having wild-caught bearded seals under the conditions offered at Polaria for several years did not markedly affect physiological parameters of the animals, and that training may have helped to alleviate stress associated with blood sampling and veterinary inspection.

Analysis of Veterinary and Sanitary Inspection in Russian Federation Subjects

The paper represents the analysis of the key parameters specifying the food safety system in the Russian Federation as of January 1, 2020. Such issues as competence of the veterinary experts and laboratories in the field of veterinary and sanitary inspection as well as availability of the necessary equipment including equipment for radiometric tests were particularly considered. Implementation of the internal audit procedure by the veterinary and sanitary testing laboratories was assessed. Data on the availability of the slaughterhouses and slaughter units/facilities for emergency slaughter as well as their staffing with the veterinarians are demonstrated. The study results indicate that 39% of the laboratories are not equipped with the necessary laboratory equipment; 8% of the laboratories perform tests using non-calibrated laboratory equipment, and only 2/3 of the laboratories are covered by the regular internal audits. Evidence of insufficient control over the attestation of the veterinarians involved in the veterinary and sanitary expertise was identified. Moreover, insufficient number of slaughter facilities and veterinary and sanitary testing laboratories in the regions of the country was highlighted as well as inadequate staffing of the laboratories with the veterinarians responsible for the official control of the compliance with the veterinary rules and technical regulations and for the veterinary and sanitary inspections. Therefore, in some regions of the country the national veterinary services lack any capacities necessary to perform the emergency slaughter of the diseased and suspect animals in the isolated and controlled environment with the subsequent on-site storage and decontamination of the slaughter products or their disposal or destruction. The study results demonstrate a number of gaps in the veterinary and sanitary inspection system thus indicating the need for corrective actions to be taken both on the federal and local levels.

Emergence of African Swine Fever in Poland (2014–2021). Successes and Failures in Disease Eradication

African swine fever (ASF) in Poland has been a major concern for pig production for the last seven years. The main constrains in ASF eradication in Poland are due to the high density of wild boar in ASF-affected areas of the country. Other factors, responsible for long-distance ASF spread to new regions of the country, are mainly related to human-mediated activity and lack of awareness of the potential disease threat to pig production. In the case of pig holdings, the only effective measure of ASF prevention is strict implementation of biosecurity rules. Despite many efforts to implement these measures, the disease occurred in a number of voivodeships, poviats and counties of Poland, primarily in eastern Poland; however, starting from 2019, also in the western part of the country. Further recommendations to eradicate ASF in Poland or at least to minimize the economic loss caused by ASF in pig production include effective wild boar population management strategies along with the implementation of strict biosecurity measures. The observations from the last seven years of ASF epizootic in Poland clearly indicate that the disease could not have been effectively controlled in wild boar population and could only be restricted in domestic pig population following severe biosecurity rules. As for ASF spread control in wild boar population, the measures applied in other EU countries include active wild boar carcass search and disposal along with sanitary and reduction hunting. These measures have also been shown non-effective. The only solution for future sustainable pig production in Poland seems to be strict collaboration between pig producers, veterinary inspection, and hunting associations.

Veterinaire volksgezondheid, een discipline in continue evolutie

Veterinary public health, a discipline in transition. Veterinary public health, a discipline in which veterinarians help control zoonotic animal diseases, has clearly contributed in improving human health. Since the early 19th century, traditional meat inspections have ensured that in many European countries, including Belgium, almost all animals slaughtered are declared fit for human consumption. Nevertheless, in recent decades, all industrialized countries have been confronted with an increasing number of foodborne infections caused by zoonotic bacteria. Until the end of the 20th century, the focus was mainly on detecting macroscopically visible abnormalities on animal carcasses and in food of animal origin. However, in farm animals, these microorganisms do not cause disease or macroscopic injuries, nor lead to reduced production parameters. The traditional veterinary inspection is therefore no longer sufficient to guarantee public health, and will, under the impulse of the European Commission, be transformed into a risk-based inspection in the near future. Furthermore, due to worldwide trading, movement of people, changed production processes and environmental degradation, a multidisciplinary approach with a renewed collaboration between animal and human medicine will, as in the past, be necessary to face these new challenges.

Genetic evidence substantiates transmission of Trichinella spiralis from one swine farm to another

BackgroundTrichinella spiralis ranks seventh in the risk posed by foodborne parasites. It causes most human cases of trichinellosis and is the most frequent cause of Trichinella outbreaks on pig farms and in wild boar, worldwide. Veterinary inspectors seek the source of outbreaks in hopes of limiting the spread. Established molecular tools are inadequate for distinguishing among potential T. spiralis infection sources because genetic variability in these zoonotic pathogens is limited in Europe. Microsatellite markers proved successful in tracing an outbreak of T. britovi, a related parasite harboring much more genetic variation. Here, we successfully employed microsatellite markers to determine the genetic structure of T. spiralis isolates from two pig outbreaks, discovering notable uniformity among parasites within each farm and discovering an epidemiological link between these two outbreaks.MethodsThe individual larvae from five isolates of T. spiralis from two pig farms and from ten wild boars were genotyped using nine microsatellite markers to examine their genetic structure.ResultsNotably uniform parasite populations constituted each farm outbreak, and the parasites from the first and second outbreaks resembled each other to a notable degree, indicating an epidemiological link between them. Wild boar harbored more genetically variable larval cohorts, distinguishing them from parasites isolated from domestic pigs.ConclusionsMicrosatellite markers succeeded in distinguishing isolates of the highly homogeneous T. spiralis, aiding efforts to track transmission. Each outbreak was composed of a homogenous group of parasites, suggesting a point source of contamination.Graphical abstract

Analysis of the prevalence of selected qualitative deviations in slaughter poultry in Poland in 2016-2019

The aim of the study was to analyse the results of official post-mortem inspections of hens, chickens, turkeys, ducks and geese carried out in poultry slaughterhouses in Poland by the Veterinary Inspection in 2016–2019. The data from the sanitary and veterinary survey were taken from annual reports drawn up by the General Veterinary Inspectorate (RRW-6) in 2016–2019. During this period, nearly 900 million hens, 206 million turkeys, 122 million ducks, 63 million geese, and over 5.7 billion slaughter chickens were slaughtered under veterinary supervision. Symptoms or lesions were found in almost 2.6 million hens (0.29%), 1.8 million turkeys (0.86%), 792 thousand ducks (0.65%), 185 thousand geese (0.29%) and over 27.3 million chickens (0.48%). The main causes of confiscation were emaciation (0.14%), incomplete loss of blood (0.06%), and septicaemia and pyaemia (0.07%). Quality deviations in the form of emaciation or incomplete loss of blood of slaughtered animals indicate shortcomings during rearing or transport of animals for slaughter. Therefore, there may be legitimate concerns about the appropriate level of animal welfare. The size of overall losses in poultry is determined by the disqualification of chickens, due to slaughter size in comparison with other species. The confiscation of such a large number of chickens deserves greater attention to determine the causes involved.

Prevalence of some foodborne parasitic affection in slaughtered animals in local Egyptian abattoir

Fascioliasis, cysticercosis and hydatidosis were aimed to be investigated in some slaughtered animals (cattle, buffalo and camel) in local Egyptian abattoir located in Cairo governorate. 10317, 763 and 290 cattle, buffalo and camel carcasses, respectively were examined in the period of 2017-2018. Results of PM inspection along the investigation period revealed detection of Fasciola in 0.58 and 1.4% of the examined cattle and buffalo carcasses, respectively, while was not detected in camel carcasses. Also, cysticercosis was detected in 0.47 and 0.69% of the examined cattle and camel carcasses, respectively; while was not detected in buffalo carcasses. In addition, hydatidosis was detected in lung and liver of 0.038 and 0.096% of cattle carcasses; 3.4 and 1.03% of camel carcasses, respectively, while was not detected in the examined buffalo samples. Annual values of financial losses because of the condemned affected parts summed total of 11712.5, 32940.0 and 2410 LE due to fascioliasis, cysticercosis and hydatidosis, respectively along the investigation period (2017 and 2018). Referring to the obtained results, records of 2017 appeared to be more infection prevalent than 2018 with more financial losses, moreover, fascioliasis was the most prominent affection in the present study. In addition, the critical veterinary inspection has a great role in protecting human-being to be infected with zoonotic meat-borne parasites. So, magnification and great support should be given to training veterinary inspectors in slaughter houses in Egypt.

Prevalence of some zoonotic parasitic affections in sheep carcasses in a local abattoir in Cairo, Egypt

Meat-borne zoonotic parasites raised from consumption of undercooked and/or crossly contaminated meats can infect human by direct and/or indirect ways causing many mild to serious diseases; so, in the present study, some meat-borne transmissible parasites were investigated in 5239 freshly dressed sheep carcasses in local Egyptian abattoir located at Cairo governorate along the period of 2017-2018. Results of Post- Mortem inspection revealed the detection of Fasciola, Cysticercus and Hydatid cyst in 3.47, 1.06 and 1.83% of the total examined carcasses with total economic losses of 9306.78 L. E, 2892 L.E and 4380 LE, respectively. It is worthily noted that fascioliasis recorded the highest incidence of infection, followed by hydatidosis and cysticercosis in the examined carcasses, respectively. Cysticercus ovis and C. tenuicollis were detected in 32.14% and 67.85% with total economic losses of 714 L.E and 2178 L.E because of infected heart and liver condemnation during 2017 and 2018, respectively. Furthermore, hydatid cyst was detected in 0.47% and 1.35% of the examined lung and liver samples with total economic losses of 1188 L.E and 3192 L.E because of infected lung and liver condemnation during 2017 and 2018, respectively. Referring to the obtained results, it was obvious that parasitic infection of sheep meat and offal constrains a lot of economic losses, threatens the meat production industry and throws lights over the health importance of veterinary inspection great role in protecting human-being from be infested with zoonotic meat-borne parasites. So, magnification and great support should be given to training veterinary inspectors in slaughter houses in Egypt.

First record of Trichinella in Leopardus guigna (Carnivora, Felidae) and Galictis cuja (Carnivora, Mustelidae): new hosts in Chile.

BackgroundTrichinellosis is a zoonotic disease with a worldwide distribution. It is caused by several species of nematodes in the genus Trichinella. Trichinella spp. are transmitted through predation or carrion consumption and occur in domestic and sylvatic cycles. In humans trichinellosis occurs due to the consumption of raw or undercooked, infected meat and is mainly associated with the household slaughter of pigs or the consumption of game animals without veterinary inspection, a cultural practice that is difficult to resolve. Therefore, knowledge of this parasite’s reservoir is relevant for better implementing public health strategies. The aim of this study was to assess the presence of Trichinella sp. in several carnivore and omnivore vertebrates in central-southern Chile.MethodsWe collected muscle tissue from a total of 53 animals from 15 species and were digested to detect Trichinella larvae which were further identified to species level using molecular techniques.ResultsWe detected Trichinella larvae in Leopardus guigna (Felidae) and Galictis cuja (Mustelidae). We identified the larvae collected from L. guigna as Trichinella spiralis, but we were unable to molecularly characterize the larvae from G. cuja. This is the first record of Trichinella in a native mustelid of South America and the first record of T. spiralis in L. guigna. This study identified two novel hosts; however, further work is needed to identify the role that these and other hosts play in the cycle of Trichinella in Chile.

Evaluation of sanitary and veterinary inspection results of slaughtered sheep in Poland in 2017-2019

The aim of this study was to follow and evaluate the sanitary and veterinary inspection results of slaughtered sheep in Poland in the period 2017-2019. More than 175,000 sheep were slaughtered in Poland during this period. Post-mortem veterinary inspection revealed symptoms and/or lesions in 19,799 animals, which constituted 11.30% of all examined sheep. Of these, 134 animals (0.08%) were considered unfit for consumption. The most numerous cases were with foci of pus, contamination and congestion (5.07%) and fasciolosis (4.72%). The highest number of foci of pus, contamination and congestion in sheep carcasses were found in the Małopolskie and Podlaskie voivodeships. Most of the cases deemed unfit for consumption were found in the Podkarpackie (30), Świętokrzyskie (29) and Podlaskie (23) voivodeships, accounting for over 61% of all disqualified sheep carcasses in 2017-2019 in Poland.

Spatial and network analysis of U.S. livestock movements based on Interstate Certificates of Veterinary Inspection

Livestock movements are a common pathway for the spread infectious diseases in a population. An understanding of livestock movement patterns is needed to understand national transmission risks of highly infectious diseases during epidemics. Social Network Analysis (SNA) is an approach that helps to describe the relationships among individuals and the implications of those relationships. We used SNA to describe the contact structure of livestock movements throughout the contiguous U.S. from April 1st, 2015 to March 31st, 2016. We describe 4 network types: beef cattle, dairy cattle, swine, and small ruminant. Livestock movement data were sourced from Interstate Certificates of Veterinary Inspection (ICVI) while county-level farm demographic data were from the National Agricultural Statistics Service (NASS). In the described networks, nodes are represented by counties and arcs by shipments between nodes; the networks were weighted based on the number of shipments between nodes. For the analyses, movement data were aggregated at the county level and on an annual basis. Measures of centrality and cohesiveness were computed and identification of trade-communities in all networks was conducted. During the study period, a total of 219,042 movements were recorded and beef cattle movements accounted for 63 % of all movements. At least 70 % of U.S. counties were present in each of the networks, but the density of arcs was less than 2% in all networks. In the beef cattle network, counties with high out-degree were strongly correlated (0.8) with the number of beef cows per county while for the dairy cattle network a strong correlation (>0.86) was found with the number of dairy cattle per km2 at the county level. All networks were found to have between 4 and 6 large communities (50 counties or more per community), and were geographically clustered except for the communities in the small ruminant network. Outputs reported in these analyses can help to understand the structure of the contact networks for beef cattle, dairy cattle, swine, and small ruminants. They may also be used in conjunction with simulation modeling to evaluate spread of highly infectious disease such as foot-and-mouth disease at the national level and to evaluate the application of intervention strategies.

The Welfare of Cattle, Sheep, Goats and Pigs from the Perspective of Traumatic Injuries Detected at Slaughterhouse Postmortem Inspection.

Simple SummaryThe occurrence of traumatic injuries was assessed in cattle, sheep, goats and pigs reared and slaughtered in the Czech Republic. For the purposes of the study, the results of veterinary postmortem examinations at slaughterhouses in the period from 2010 to 2019 were analyzed. In the studied animal species, findings of traumatic lesions were detected at low frequency. The low frequency of traumatic lesions is favorable from the perspective of the welfare of slaughtered animals. In terms of further improvements to animal welfare, it would be desirable to focus on the prevention of trauma in cattle in particular, in which findings of trauma were more frequent than in the other species studied. The category most affected by trauma both to the limbs and body was cows.The welfare of cattle, pigs, sheep and goats was assessed by measuring trauma detected during veterinary postmortem inspection at slaughterhouses. The subject of this evaluation were all bovine, porcine, ovine and caprine animals slaughtered at Czech slaughterhouses in the monitored period, i.e., a total of 1,136,754 cows, 257,912 heifers, 1,015,541 bulls, 104,459 calves, 586,245 sows, 25,027,303 finisher pigs, 123,191 piglets, 22,815 ewes, 114,264 lambs, 1348 does and 5778 kids. The data on the numbers of traumatic findings were obtained retrospectively from a national veterinary database collecting data from slaughterhouse postmortem examinations. The results showed that findings of trauma were observed at a low frequency in the studied species. Injuries were detected most frequently in cows (1.71%). In contrast, no findings associated with the presence of trauma were recorded in does and kids. From the viewpoint of trauma localization, findings on the limbs were more frequent than findings on the body (p < 0.01). The only exceptions to this were lambs, does and kids, for which there was no statistically significant difference between findings on the limbs and the body (p = 1.00). The results show that housing system (bedding, the presence of slats, floor hardness), transport of animals to the slaughterhouse (moving animals to the vehicle, loading ramps, floors in transport vehicles and the transport of animals itself) and design of the slaughterhouse (unloading ramps, passageways and slaughterhouse floors) have a greater impact on the limbs than the bodies of animals in the majority of species. A difference was also demonstrated in the occurrence of findings of trauma in the limbs and body (p < 0.01) between culled adult animals and fattened animals, namely in cattle and pigs. A difference (p < 0.01) between ewes and lambs was found only in the occurrence of traumatic injury to the limbs. The results showed that fattened animals are affected by the risk of trauma to a lesser extent than both culled adult animals and young animals. Statistically significant differences (p < 0.01) were also found between the studied species and categories of animals. The category most affected from the viewpoint of injury both to the limbs and body was cows. In contrast to cows that are typically reared indoors, the low frequency of traumatic findings was found in small ruminants and in bulls, i.e., animals typically reared outdoors. Assumedly, access to pasture may be beneficial considering the risk of traumatic injury.

Atypical Manifestation of Cutaneous Fowlpox in Broiler Chickens Associated with High Condemnation at a Processing Plant

The present case is an unusual report of cutaneous fowlpox with an atypical appearance and incidence in broilers. Gross skin lesions were noticed in 41-day-old commercial broilers during the veterinary inspection at a processing plant in the north of Iran. The skin lesions were only observed on feathered skin areas of the broilers and remained unnoticed until slaughter. Round, nodular or coalescent, elongated, reddish-brown proliferative lesions were mainly located on the back, thighs, and proximal areas of the neck of broilers. Nonfeathered skin, including the wattle, comb, eyelids, and legs, were not affected. This condition incurred high losses due to a 5.3% condemnation and trimming of carcasses. Cutaneous lesions were sampled for histopathology and molecular virology for further investigations. Histopathology revealed multifocal necrotic dermatitis with epidermal eosinophilic cytoplasmic inclusion bodies in the skin lesions. Molecular investigations confirmed the presence of fowlpox virus (FWPV) in the proliferative lesions, with further investigations identifying two FWPV genome populations, one carrying a portion of the reticuloendotheliosis virus (REV) and the other a nearly complete REV provirus. Furthermore, the 4b core protein gene-based molecular analysis clustered the field virus into clade A of the genus Avipoxvirus.