The lipid bilayer structure is the main component of cell membranes, therefore, the physical properties of such structure have been studied for many years. Recently, the applications of lipid bilayers have been studied, in addition to fundamental studies. For instance, a transistor-like device was developed (Ma et al., ACS Omega, 2019).As a part of such studies, we show the 2D and 3D control of lipid bilayer membranes. The first one is the effect of local anesthetics, Dibucaine hydrochloride molecules, on the phase behavior of ternary lipid membranes, which corresponds to the 2D control of lipid bilayer membranes (Yoshida et al., MedChemComm, 2015). The lipid membranes composed of a saturated phospholipid, unsaturated phospholipid, and cholesterol induce lateral phase separation. We revealed that the phase behavior is affected by the insertion of local anesthetic molecules into bilayer structures. Another one is the effect of toxic polar molecules, acetonitrile molecules, on the shape of lipid bilayer vesicles, which corresponds to the 3D control of lipid bilayer membranes (Yoshida et al., Colloids Interfaces, 2018). Insertion of acetonitrile molecules induces the membrane deformation.We are trying to utilize such behaviors for soft and bio-inspired molecular sensors. We show the trials for such applications.