The environmental effect resulting from dam construction and the formation of large reservoirs has long been a topic of significant concern. While current research at a macro scale provides a well understanding regarding the evolution of nitrogen nutrient status in river-reservoir ecosystems, the vertical migration and transformation characteristics of nitrogen in reservoirs under changing runoff remain less defined. This study centers on a typical deep reservoir along the Lancang River-Nuozhadu (NZD) Reservoir in April and December. The monitoring reveals a distinct vertical stratification phenomenon in the NZD Reservoir in the vertical profile in April, which can be categorized into a mixed layer (ML) (0–5 m), a thermocline layer (TL) (5–40 m), and an isothermal layer (IL) (>40 m) based on the water temperature. However, little stratification in the profile is occurred in December. The results of nitrogen and oxygen isotopes in April indicate that the biochemical process of nitrogen in the NZD Reservoir is predominantly driven by nitrification. However, different nitrogen biochemical processes are coupled in various stratified regions in the vertical direction. In the ML, simultaneous assimilation of ammonia nitrogen by phytoplankton occurs. In the TL, there is a process of oxidation and decomposition of particulate organic nitrogen due to a significant stratification effect and an extended hydraulic retention time. In the region of minimal dissolved oxygen (30–40 m), simultaneous denitrification may take place. In December, the main process is nitrogen assimilation in reservoir surface (ML). Statistical analysis of environmental factors highlights water temperature (T) and dissolved oxygen (DO) as crucial environmental factors influencing nitrogen migration and transformation within the reservoir. This study suggests that in deep reservoirs experiencing vertical thermal stratification, the characteristics of DO and nitrogen migration and transformation exhibit significant vertical spatial heterogeneity. This phenomenon may lead to diverse ecological environmental effects. Future efforts should focus on enhancing the fine monitoring of stratified water bodies in deep and large reservoirs and analyzing the ecological environmental effects of material cycling in greater detail.
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