Anterior cruciate ligament injuries have become commonplace among female athletes, with more than 70% of injuries resulting from noncontact mechanisms. Muscular fatigue likely contributes to knee injury risk due to adaptations that occur during intense exercise. There is limited research on high intensity fatigue protocols occurring on consecutive days that may better mimic competitive sport. PURPOSE: To compare knee flexion angle, knee flexion moment, and vertical leg stiffness during single-leg jump landing measured immediately after high intensity bout of fatiguing exercise. METHODS: 12 female varsity soccer players (Age = 19.25 ± 1.36 years, Height = 170 ± 6.96cm, Weight = 62.36 ± 8.53kg) were randomly assigned to control and fatigue groups. Both groups completed five single-leg drop jumps from a 30cm height, landing on their dominant leg, defined as the kicking leg, every day for four days. The first day was completed without intervention and the fatigue group completed consecutive fatigue protocols before the drop jumps on days two through four. The fatigue protocols included 100 squat jumps, 100 lunge jumps, and 100 burpees, on days two, three, and four, respectively. Exercises were completed in five sets, 20 repetitions in each, with a 50m sprint between sets. Motion capture and force plate data were used to calculate percentage of change in knee flexion angle (°), knee flexion moment (Nm/kg), and vertical stiffness (kN/m) compared to pre-fatigue values. A Mann-Whitney U Test (data were not normally distributed) was used to compare differences between groups. RESULTS: There were no statistically significant differences between groups. Knee flexion angle remained constant to pre-fatigue values (4.24 ± 14.6%, -1.37 ± 15.96%, 4.17 ± 19.77%) on days two through four, respectively. No significant changes in knee moment were noted after three days of exercise (37.95 ± 38.37%, -12.69 ± 59.52%, 52.35 ± 124.22%). Increases in stiffness were not statistically significant after three days of exercise (38.34 ± 66.84%, 6.32 ± 54.43%, 25.79 ± 30.81%). CONCLUSION: A short bout of high intensity exercises did not alter dominant leg landing mechanics during single leg landing. The duration and mode of exercise used in this experiment did not cause a significant alteration in landing biomechanics on any of the testing sessions.