AbstractWe use in situ measurements of suspended mud to assess the flocculation state of the lowermost freshwater reaches of the Mississippi River. The goal of the study was to assess the flocculation state of the mud in the absence of seawater, the spatial distribution of floc sizes within the river, and to look for seasonal differences between summer and winter. We also examine whether measured floc sizes can explain observed vertical distributions of mud concentration through a Rouse profile analysis. Data were collected at the same locations during summer and winter at similar discharges and suspended sediment concentrations. Measurements showed that the mud in both seasons was flocculated and that the floc size could reasonably be represented by a cross‐sectional averaged value as sizes varied little over the flow depth or laterally across the river at a given station. Depth‐averaged floc sizes ranged from 75 to 200 microns and increased slightly moving downriver as turbulence levels dropped. On average, flocs were 40 microns larger during summer than in winter, likely due to enhanced microbial activity associated with warmer water. Floc size appeared to explain vertical variations in mud concentration profiles when the bed was predominately composed of sand. Average mud settling velocities for these cases ranged from 0.1 to 0.5 mm/s. However, Rouse‐estimated settling velocities ranged from 1 to 3 mm/s at two stations during winter where the bed was composed of homogeneous mud. These values exceeded the size‐based estimates of settling velocity.