Vertebrate Lamins Research Articles

The Triton X-100 and High Salt Resistant Residue of Saccharomyces cerevisiae Nuclear Membranes, II / Isolation of the Nuclear Membrane Insoluble Residue from G1 Arrested Cells and Immunological Comparison with the Corresponding Vertebrate Nuclear Fraction

Abstract Isolation of the Nuclear Membrane Insoluble Residue from G1 Arrested Cells and Immunological Comparison with the Corresponding Vertebrate Nuclear Fraction The Triton X-100 and High salt resistant residue of the nuclear envelope of Saccharomyces cerevisiae cells arrested in the G 1 phase of cell cycle with a mating pherom one was isolated. It showed the same electrophoretic protein pattern as the insoluble residue isolated from log-phase cells. Examination of nitrocellulose blots of nuclear protein fractions with antiserum raised against a vertebrate lamin failed to show any immunological cross-reaction.