Versatile Technology Research Articles

A NOVEL FAULT-TOLERANT GENERALIZED SYMMETRICAL TOPOLOGY FOR RENEWABLE ENERGY AND ELECTRIC VEHICLE APPLICATIONS

Multilevel inverters are a versatile and powerful technology for applications such as motor drives, high voltage direct current transmission (HVDC), renewable energy systems, and grid-connected applications. As the demand for high-quality, clean energy and low distortion continues to grow, multilevel inverters are preferred over traditional square wave inverters. This paper proposes a generalized 13-level symmetrical topology to meet the power quality requirements. It consists of six DC sources and twelve semiconductor devices. Further, the topology has cascaded to increase the number of levels, but the cost and circuit complexity increase with the number of units. The gate pulses for switches are produced by the nearest-level modulation technique. The performance comparison of the topology with recent literature provides comprehensive information about the novelty of the configuration. The MLI performance parameters are number of switches, number of gates driving circuits, total harmonic distortion (THD), cost function per level (CCPL), total standing voltage (TSV), and efficiency (η). MATLAB/Simulink investigates the proposed topology, and further, to validate its simulation output, the hardware prototype model has been implemented in the laboratory.

Integration of CRISPR/Cas9 with multi-omics technologies to engineer secondary metabolite productions in medicinal plant: Challenges and Prospects.

Plants acts asliving chemical factories that may create a large variety of secondary metabolites, most of which are used in pharmaceutical products. The production of these secondary metabolites is often much lower. Moreover, the primary constraint after discovering potential metabolites is the capacity to manufacture sufficiently for use in industrial and therapeutic contexts. The development of omics technology has brought revolutionary discoveries in various scientific fields, including transcriptomics, metabolomics, and genome sequencing. The metabolic pathways leading to the utilization of new secondary metabolites in the pharmaceutical industry can be identified with the use of these technologies. Genome editing (GEd) is a versatile technology primarily used for site-directed DNA insertions, deletions, replacements, base editing, and activation/repression at the targeted locus. Utilizing GEd techniques such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 (CRISPR-associated protein 9), metabolic pathways engineered to synthesize bioactive metabolites optimally. This article will briefly discuss omics and CRISPR/Cas9-based methods to improve secondary metabolite production in medicinal plants.

Enhanced activity of split trehalase biosensors by interspecies domain combineering.

The split trehalase biosensor has potential as a versatile diagnostic technology. Split enzymes are engineered proteins, divided into inactive fragments, which can reassemble and regain activity when brought together by an analyte. The split TreA biosensor requires no sample processing and produces stable signals (in the form of glucose). Split trehalase reagents can function in blood, but periplasmic trehalase of E.coli requires blood acidification for maximal activity. The objective of this study was to obtain split trehalase with near physiological pH optimum. For this purpose, periplasmic trehalases of Cellvibrio spp. with higher activity at neutral pH, were split in analogy with the E. coli TreA into hood and catalytic domains. However, these split trehalases displayed self-complementation due to spontaneous reassembly. In contrast, when catalytic domains of Cellvibrio trehalases were combined with E.coli hood domains, these hybrids displayed conditional complementation capacity when split trehalase fragments fused to immunoglobulin-binding protein G (STIGA) were used to quantify immunoglobulin concentrations. Other hybrid combinations of Cellvibrio spp. had increased activity compared to the cognate pairs, albeit with strong self-complementation. A mutagenesis analysis of residues responsible for self-complementation led to uncoupling of self-complementation from allostery. The Michaelis-Menten kinetics of Cellvibrio enzymes and fragment pairs confirmed improved activity of a mutated hybrid pair of Cellvibrio hood and catalytic domains at physiological pH. In conclusion, the improvements in pH optimum and activity, demonstrated with STIGA, can now be leveraged to enhance other variations of the split trehalase biosensor platform, broadening its utility for testing clinical samples.

Lipid Nanoparticle-Mediated Oip5-as1 Delivery Preserves Mitochondrial Function in Myocardial Ischemia/Reperfusion Injury by Inhibiting the p53 Pathway.

Myocardial ischemia/reperfusion (MI/R) injury, a major contributor to poor prognosis in patients with acute myocardial infarction, currently lacks effective therapeutic strategies in clinical practice. The long noncoding RNA (lncRNA) Oip5-as1 can regulate various cellular processes, such as cell proliferation, differentiation, and survival. Oip5-as1 may have potential as a therapeutic target for MI/R injury as its upregulated expression has been associated with reduced infarct size and improved cardiac function in animal models, although how to effectively and safely overexpress Oip5-as1 in vivo remains unclear. Lipid nanoparticles (LNPs) are a versatile technology for targeted drug delivery in numerous therapeutic applications. Herein, we aimed to assess the therapeutic efficacy and safety of LNPs coloaded with Oip5-as1 and a cardiomyocyte-specific binding peptide (LNP@Oip5-as1@CMP) in a murine model of MI/R injury. To achieve this, LNP@Oip5-as1@CMP was synthesized via ethanol injection method. The structural components of LNP@Oip5-as1@CMP were physicochemically analyzed. A hypoxia/reoxygenation (H/R) model in HL-1 cells and coronary artery ligation in mice were used to simulate MI/R injury. Our results demonstrated that LNPs designed for cardiomyocyte targeting and efficient Oip5-as1 delivery were successfully synthesized. In HL-1 cells, LNP@Oip5-as1@CMP treatment significantly reduced mitochondrial apoptosis caused by H/R injury. In the murine MI/R model, the intravenous administration of LNP@Oip5-as1@CMP significantly decreased myocardial infarct size and improved cardiac function. Mechanistic investigations revealed that Oip5-as1 delivery inhibited the p53 signaling pathway. However, the cardioprotective effects of Oip5-as1 were abrogated by administrating Nutlin-3a, a p53 activator. Furthermore, no signs of major organ damage were detected after LNP@Oip5-as1@CMP injection. Our study reveals the therapeutic potential of LNPs for targeted Oip5-as1 delivery in mitigating MI/R injury. These findings pave the way for advanced targeted treatments in cardiovascular diseases, emphasizing the promise of lncRNA-based therapies.

Fused Deposition Modeling-Based 3D Printing as a Versatile Technology to Manufacture Vaginal Films Incorporating Metronidazole

Fused Deposition Modeling-Based 3D Printing as a Versatile Technology to Manufacture Vaginal Films Incorporating Metronidazole

Unveiling exotic multi-scale microstructure transformation and crack formation mechanisms in eutectic ceramic composite by laser powder bed fusion

Laser powder bed fusion (LPBF) represents an advanced and versatile technology. Exploiting its distinctive advantages for direct and rapid fabrication of complex-structured melt-grown oxide eutectic ceramic composite represents a pioneering yet challenging endeavor. In this work, LPBF is creatively employed to fabricate turbine blade-shaped, in-situ ternary eutectic ceramic composite. Through the integration of experimental procedures, Finite element method (FEM) simulations, and numerical analyses, an innovative design and manufacturing of oxide eutectic ceramic composites have been successfully established. Comprehensive FEM simulations, with detailed interface characteristic analysis, have revealed macro-scale cracks induced by intense maximum principal stress, and micro-cracks stemming from significant interfacial energy disparities among the three constituent phases. The applications of rapid solidification and nucleation theories have facilitated profound insights into the formation mechanisms of multi-scale exotic microstructures, including top-layer coarse dendrites, rosette-like spherical internally grown eutectic colony within layers, and columnar eutectic colonies with ultra-fine lamellar eutectic structures. Micro-mechanical property testing reveals enhanced performance in the inter-layer ultra-fine lamellar eutectic structure, which is attributed to a refined eutectic spacing of approximately 61 nm, coupled with distinct and robust bonding interfaces. These groundbreaking achievements, focusing on the processing-microstructure-property relationship in the fabrication of gas turbine blade-shaped solidified eutectic ceramic composite using LPBF, provide invaluable theoretical insights and data. This knowledge is crucial for the LPBF production of high-temperature structural materials, highlighting its significant potential applications in fields of aerospace and mechanical engineering.

Internet use at work and income inequality in Ecuador

This study examines the impact of technological advancements, particularly Internet access, on labor income inequality. The Internet is a versatile technology with wide-ranging applications. We argue that in environments characterized by limited innovation and a polarized job market, Internet access can positively influence worker income, especially for those in lower income brackets. Our research conducted in Ecuador from 2010 to 2017 employs instrumental variable quantile regression to analyze how Internet access affects wages across various income levels. Our findings indicate that using the Internet at work correlates with higher wages in Ecuador, particularly benefiting workers in the higher quantiles of the income distribution. While the income gap between employees with and without Internet access is largest in the lowest quantile, it decreases in the second quantile and widens again in the third, but never surpasses the gap observed in the first quantile. Public policy should thus provide citizens, particularly in the lowest income deciles, with education on effective Internet usage to improve their conditions and reduce inequality. By concentrating on these groups, we can better distribute the advantages of Internet access, ultimately creating a more balanced and equitable society.

Sustainable Wastewater Treatment Strategies in Effective Abatement of Emerging Pollutants

The fundamental existence of any living organism necessitates the availability of pure and safe water. The ever-increasing population has led to extensive industrialization and urbanization, which have subsequently escalated micropollutants and water contamination. The environmental impact on various life forms poses a dire need for research in effective environmental management. Versatile technologies involving multiple approaches, including physiochemical and biological bioremediation strategies, draw insights from environmental biology. Metabolic annihilation mediated by microbes shows significant potential in the bioconversion of toxic micropollutants to tolerable limits. Environmentally friendly, cost-effective, and sustainable strategies are envisaged for efficient environmental protection. Phytoremediation technology, especially floating wetland treatments, facilitates micropollutant elimination, landscape management, ecosystem conservation, and aesthetic enhancement in diverse environments. The incorporation of nanomaterials in the bioremediation of toxic micropollutants augments novel and innovative strategies for water pollution abatement. This paper offers a novel strategy that combines nanomaterials to improve micropollutant degradation with bioremediation techniques, particularly the creative application of phytoremediation technologies like floating wetlands. Combining these techniques offers a novel viewpoint on long-term, affordable approaches to reducing water pollution. Additionally, the review proposes a forward-looking strategic framework that addresses the accumulation and refractory nature of micropollutants, which has not been thoroughly explored in previous literature.

External Support for Solar-Powered Water Pumping Systems in Rural Areas: A Systematic Review.

The Sustainable Development Goals emphasize coordination and integration between sectors. Solar-powered submersible water pumping systems are versatile technology that help address community drinking water, irrigation, and electricity needs. Stakeholders external to the community, particularly solar photovoltaic experts, are vital in ensuring continued system services; however, there has been no comprehensive assessment of different solar-powered water pumping system support efforts. This review is the first to systematically evaluate external support for solar-powered systems from multiple regions and implementing organizations. We reviewed solar-powered water pumping system literature to identify implemented external support and factors that affect implementation. Publication databases, organization Web sites, and citations were searched. Seventy-four studies were included and evaluated using inductive coding and thematic synthesis. We derived a framework that organized support activities and factors into three nested levels of implementation: system, program, and sector. For support efforts implemented after 2010, most support providers worked at all levels. Each provider type worked at levels aligned with their knowledge and resources and complementary to other providers' work. Drivers of support specific to solar-powered water systems were the existence of solar photovoltaic markets and infrastructure, support providers experienced with solar photovoltaics, and government and community solar advocates. We grouped support factors that study authors associated with system functionality into four categories: location and quality of support, reliability of support arrangements, frequency and timeliness of support, and policy and regulatory environment. No study outlined support for multiple uses of the systems or end-of-lifecycle care of solar panels. Solar-powered water pumping systems provide multiple community services, and their management will be bolstered by support providers collaborating to optimally apply their skill sets and create support plans that comprehensively address system versatility.

Domain adaptation between heterogeneous time series data: A case study on real-time rotary machinery fault diagnosis

Artificial intelligence (AI) in fault diagnosis has emerged as a significant tool for addressing anomalies in rotary machineries (RMs). However, the success of AI models in this context largely depends on situations where the distribution of samples in the training dataset closely aligns with that of the testing dataset. In addition, practically, it is very difficult, costly, or even impossible to collect sufficient time series samples with proper labeling for AI modeling. To address these issues, this paper presents a study developing a structured methodology called frequency-domain supervised domain adaptation (FDSDA) considering heterogeneous data from versatile sensor technologies. In the context of application, two high-dimensional datasets were generated, each following distinct distributions: one being the vibration signals and the other the acoustic signals. The modeling begins with transforming signals from the time domain to the frequency domain through frequency analysis. Subsequently, a domain adaptation model is implemented, adopting an ensemble learning as an estimator to address the covariate shift challenge. Two experiments are conducted with the proposed method for binary and multi-class fault diagnosis of RMs. The results demonstrate that, despite having limited training and target data, the proposed method can effectively detect RMs fault conditions by outperforming the benchmark method.

Enumeration and gentle sorting of immune cells on chip, key to next generation advanced therapies in outpatient setting

BackgroundA thorough understanding of immune-oncology and molecular medicine has been vital in the development of cell therapeutics. At the basis of this translational research and its future implementation into a medicinal product, lies the availability of pure and viable cell populations. Currently, FACS and magnetic bead isolation are successfully used but suffer to fulfill all requirements. FACS is costly and difficult to upscale due to the limitation of shear stress, especially fragile, cells can handle. Therefore, magnetic bead isolation is often used as it is gentler, but it lacks the multiparametric aspect to isolate more complex cellular profiles. AimsWe aim to develop a versatile technology able of multi marker detection and isolation of complex cell types with high purity, viability and throughput. MethodsWe have developed a gentle sorting mechanism based on a jet flow created by micro vapor bubbles, enabling a closed microfluidic cell isolation platform capable of multiparametric sorting with high viability, purity and throughput. In this work we compared the purity, recovery and viability of sorted CD4+ CD14- cells to magnetic isolation, most often used for other cell manufacturing approaches. Futhermore, we cultured the sorted cells of both isolation strategies and compared their growth curve and expression of activation-induced IL2 and IFN-γ. ResultsWe demonstrate that this tool can achieve a pure population of CD4+ CD14- cells with high viability after sorting without compromising the recovery. On top of the viability also the growth and activation potential of sorted cells is unhampered by comparison to the benchmark gentle magnetic isolation. ConclusionsOur technology allows for the development of a compact system which sets it apart from other efforts intended to create automated cell therapeutic solutions.

NOVEL APPROACH TO PEDIATRIC ANTI-NAUSEA: FORMULATING & EVALUATING ONDANSETRON-INFUSED CHOCOLATE

Ondansetron is a widely used anti-emetic for managing nausea and vomiting caused by chemotherapy, gastroenteritis, motion sickness, and morning sickness. Traditional formulations include dispersible tablets, syrups, and injections, but these can be challenging for pediatric patients due to the bitter taste of ondansetron. To enhance palatability and patient compliance, we propose the development of a novel dosage form: ondansetron-infused chocolate. This innovative approach leverages chocolate’s natural appeal to pediatric patients, effectively masking the drug’s bitterness. By utilizing a chocolate base, we aim to improve the acceptability of ondansetron, thereby ensuring better adherence to treatment regimens. This chocolate drug delivery system represents a versatile and child-friendly technology, potentially transforming how pediatric anti-emetic therapy is administered.

Aluminide Coatings by Means of Slurry Application: A Low Cost, Versatile and Simple Technology

The present study focused on demonstrating the versatility of the slurry deposition technique to produce aluminide coatings to protect components from high-temperature corrosion in a broad temperature range, from 400 to 1400 °C. This is a simpler and low-cost coating technology used as an alternative to CVD and pack cementation, which also allows the coating of complex geometries and offers improved and simple repairability for a lot of industrial applications, along with avoiding the use of non-hazardous components. Slurry aluminide coatings from a proprietary water-based-Cr6+ free slurry were produced onto four different substrates: A516 carbon steel, 310H AC austenitic steel, Ti6246 Ti-based alloy and TZM, a Mo-based alloy. The resulting coatings were thoroughly characterised by FESEM and XRD, mainly so that the identification of microstructures and appropriate phases was reported for each coating. The importance of surface preparation and heat treatment as key parameters for the coating final microstructures was also evidenced, and how those parameters can be optimised to obtain stable intermetallic phases rich in Al to sustain the formation of a protective Al2O3 oxide scale. These coating systems have applications in diverse industrial environments in which high-temperature corrosion limits the lifetime of the components.

Advanced Electrospun Composites Based on Polycaprolactone Fibers Loaded with Micronized Tungsten Powders for Radiation Shielding.

Exposure to high levels of radiation can cause acute, long-term health effects, such as acute radiation syndrome, cancer, and cardiovascular disease. This is an important occupational hazard in different fields, such as the aerospace and healthcare industry, as well as a crucial burden to overcome to boost space applications and exploration. Protective bulky equipment made of heavy metals is not suitable for many advanced purporses, such as mobile devices, wearable shields, and manned spacecrafts. In the latter case, the in-space manufacturing of protective shields is highly desirable and remains an unmet need. Composites made of polymers and high atomic number fillers are potential means for radiation protection due to their low weight, good flexibility, and good processability. In the present work, we developed electrospun composites based on polycaprolactone (polymer matrix) and tungsten powder for application as shielding materials. Electrospinning is a versatile technology that is easily scalable at an industrial level and allows obtaining very lightweight, flexible sheet materials for wearables. By controlling tungsten powder size, we engineered homogeneous, stable and processable suspensions to fabricate radiation composite shielding sheets. The shielding capability was assessed by an in vivo model on prototype composite sheets containing 80 w% of W filler in a polycaprolactone (PCL) fibrous matrix by means of irradiation tests (X-rays) on mice. The obtained results are promising; as expected, the shielding effectivity of the developed composite material increases with the thickness/number of stacked layers. It is worth noting that a thin barrier consisting of 24 layers of the innovative shielding material reduces the extent of apoptosis by 1.5 times compared to the non-shielded mice.

Parallel reaction monitoring targeted mass spectrometry as a fast and sensitive alternative to antibody-based protein detection

The reliable, accurate and quantitative targeted detection of proteins is a key technology in molecular and cell biology and molecular diagnostics. The current golden standard for targeted protein detection in complex mixtures such as complete cell lysates or body fluids is immunoblotting, a technology that was developed in the late 1970s and has not undergone major changes since. Although widespread, this methodology suffers from several disadvantages, such as the inability to detect low-abundant proteins or specific posttranslational modifications, the requirement for highly specific antibodies, the lack of quantitative power and the often-tedious practical procedures. Mass spectrometry (MS) based targeted protein detection is an alternative technology that could circumvent these caveats. Here, we compare immunoblotting with targeted protein mass spectrometry using a parallel reaction monitoring (PRM) regime on the Orbitrap mass spectrometer. We show that PRM based MS has superior sensitivity and quantitative accuracy over immunoblotting. The limit of detection for proteolytic peptides of a purified target protein was found to be in the mid- to low-attomole range and approximately one order of magnitude higher when embedded in a complex biological matrix. The incorporation of synthetic heavy isotope labeled (AQUA) peptides as internal calibrants into the PRM workflow allows for even higher accuracy for both the relative and absolute quantitation of tryptic target peptides. In conclusion, PRM is a versatile and sensitive technology, which can overcome the shortcomings of immunoblotting. We argue that PRM based MS could become the method of choice for the targeted detection of proteins in complex cellular matrices or body fluids and may eventually replace standard methods such as Western blotting and ELISA in biomedical research and in the clinic.

An investigation on the wear properties of the photocurable components produced by additive manufacturing for dentistry applications: Combined influences of UV exposure time, building direction, and sliding loads

AbstractAdditive manufacturing (AM) of polymers is a highly versatile technology that can be applied to many independent sectors like automotive, aviation, medicine, and dentistry. Since it has great potential for rapid prototyping, clean‐process concepts, and the ability to produce complex shapes, the layer‐by‐layer printing method is one of the most promising alternatives for future industrial production efforts. In that sense, different from the previous studies, this work aims to elucidate the friction and wear properties of the special dental samples manufactured via photopolymerization‐based AM technology according to both for printing parameters, and dry sliding test variables. Also, this is the first initiation to examine the combined influences of the UV exposure time, building direction, and sliding force on the surface roughness, hardness, friction coefficient, wear rate, and main plastic damage mechanism of the printed samples. The results showed that the maximum average hardness value was detected as 89.8 Shore D for vertically built samples printed with 8 s exposure time. In addition, vertically printed samples exhibited better wear resistance than the horizontal samples and the rising exposure time generally affected affirmatively the hardness levels of the samples. The lowest volume loss of 78 mm3 belonged to the vertical sample at 5 N. Further, increasing test force levels caused a decrease in the friction coefficient results and triggered the volume loss increase in the samples. Among all samples, the calculated friction coefficient values changed between 0.3 and 0.87. On the other side, scanning electron microscopy (SEM), and energy‐dispersive spectroscopy (EDS) analyses pointed out that ascending exposure times led to the altering contact surface matchings determining the final volume loss outcomes.Highlights To obtain better surface quality, vertical printing was a useful option. Horizontally printed samples exhibited higher friction coefficients. Curing time positively impacted the wear resistance for both orientations. Grooves and debris parts were observed on surfaces with low exposure times.

Enhancing sustainable energy production through biomass gasification gas technology: a review

This proposed research investigates the sustainable and innovative use of biomass gasification for generating electricity. Biomass gasification is a versatile and eco-friendly technology that converts organic materials, such as agricultural residues, forestry waste, and even municipal solid waste, into a valuable source of clean energy. This research delves into the various aspects of this technology, including its processes, efficiency, environmental impact, and potential applications in power generation. Biomass gasification gas, often referred to as syngas, presents a promising avenue for addressing the rising energy demand while lowering greenhouse gas emissions and preventing climate change. This research seeks to offer a thorough insight into the principles and practices behind biomass gasification, highlighting its role in the transition towards a sustainable and renewable energy future. The research will investigate the technical and economic feasibility of utilizing biomass gasification gas for electricity generation, examining the benefits, challenges, and opportunities associated with this alternative energy source. By addressing critical issues such as feedstock availability, gasifier technology, gas cleaning processes, and power plant integration, this study seeks to offer valuable insights into the potential of biomass gasification gas as a clean and renewable energy solution.

Liver-on-a-Chip Integrated with Label-Free Optical Biosensors for Rapid and Continuous Monitoring of Drug-Induced Toxicity.

Drug toxicity assays using conventional 2D static cultures and animal studies have limitations preventing the translation of potential drugs to the clinic. The recent development of organs-on-a-chip platforms provides promising alternatives for drug toxicity/screening assays. However, most studies conducted with these platforms only utilize single endpoint results, which do not provide real-time/ near real-time information. Here, a versatile technology is presented that integrates a 3Dliver-on-a-chip with a label-free photonic crystal-total internal reflection (PC-TIR) biosensor for rapid and continuous monitoring of the status of cells. This technology can detect drug-induced liver toxicity by continuously monitoring the secretion rates and levels of albumin and glutathione S-transferase α (GST-α) of a 3D liver on-a-chip model treated with Doxorubicin. The PC-TIR biosensor is based on a one-step antibody functionalization with high specificity and a detection range of 21.7ngmL-1 to 7.83 x 103ngmL-1 for albumin and 2.20ngmL-1 to 7.94 x 102ngmL-1 for GST-α. This approach provides critical advantages for the early detection of drug toxicity and improved temporal resolution to capture transient drug effects. The proposed proof-of-concept study introduces a scalable and efficient plug-in solution for organ-on-a-chip technologies, advancing drug development and in vitro testing methods by enabling timely and accurate toxicity assessments.

Electrocatalysis in MOF Films for Flexible Electrochemical Sensing: A Comprehensive Review.

Flexible electrochemical sensors can adhere to any bendable surface with conformal contact, enabling continuous data monitoring without compromising the surface's dynamics. Among various materials that have been explored for flexible electronics, metal-organic frameworks (MOFs) exhibit dynamic responses to physical and chemical signals, offering new opportunities for flexible electrochemical sensing technologies. This review aims to explore the role of electrocatalysis in MOF films specifically designed for flexible electrochemical sensing applications, with a focus on their design, fabrication techniques, and applications. We systematically categorize the design and fabrication techniques used in preparing MOF films, including in situ growth, layer-by-layer assembly, and polymer-assisted strategies. The implications of MOF-based flexible electrochemical sensors are examined in the context of wearable devices, environmental monitoring, and healthcare diagnostics. Future research is anticipated to shift from traditional microcrystalline powder synthesis to MOF thin-film deposition, which is expected to not only enhance the performance of MOFs in flexible electronics but also improve sensing efficiency and reliability, paving the way for more robust and versatile sensor technologies.

Laser-patterning bacterial nanocellulose for cell-controlled interaction

The interfacial topography of biomaterials has been identified as a major biophysical regulator of cell behavior and function, a role played through the interplay with biochemical cues. In this work, we demonstrate the potential of laser as a versatile technology for the direct fine-tuning of the topography of Bacterial nanocellulose (BNC) with bioinspired topographies and micropatterns on a cell size scale. Two lasers were used, with different wavelengths—IR (CO2, 10600 nm) and UV (tripled Nd: YVO4, 355 nm) —attempting to reproduce the Pitcher-plant topography and to create cell-contact guidance patterns, respectively. Different topographies with parallel grooves featuring a 20–300 μm period were generated on the BNC surface with high fidelity and reliability of the generated microstructures, as demonstrated by 3D optical profilometry and scanning electron microscopy. Moreover, it was demonstrated by X-ray photoelectron spectroscopy that laser processing does not result in detectable chemical modification of BNC. The developed anisotropic microstructures can control cell behavior, particularly regarding morphology, alignment, and spatial distribution. Thus, this proof-of-concept study on the high-resolution laser patterning of BNC opens new perspectives for the development of cell-modulating laser-engineered BNC interfaces, scaffolds, and other advanced medical devices, which can potentially broaden the application of BNC in the biomedical field.