A versatile speech manipulation method, STRAIGHT (Speech Transformation and Representation using Adaptive Interpolation of weiGHTed spectrogram) [Kawahara, ICASSP (1997)], was applied to natural speech in order to test the effects of speech parameter manipulations. STRAIGHT consists of three procedures called STRAIGHT-core, TEMPO (Temporal domain Excitation extraction using a Minimum Perturbation Operator) and SPIKES (Synthetic Phase Impulse for Keeping Equivalent Sound). STRAIGHT-core is a method to extract a smoothed time-frequency representation, which is free from interferences due to the source periodicity. TEMPO is used to extract F0 and other source-related information. SPIKES provides artificial ‘‘naturalness’’ to the synthetic speech. As a base line, resynthesized speech, using the STRAIGHT method, was found to provide equivalent ‘‘naturalness’’ compared to the original speech when no parametric modification was introduced. Simultaneous manipulation of the spectral envelope and F0 illustrated that stretching the frequency axis of the spectral envelope, using the quantity of the cubic root of F0 stretching, preserved ‘‘naturalness’’ the best. Perceptual effects of group delay used in the SPIKES procedure were also investigated. [Work supported by CREST (Core Research for Evolutional Science and Technology) of Japan Science and Technology Corporation (JST).]