Verification Program Research Articles

Kaempferol inhibits colorectal cancer metastasis through circ_0000345 mediated JMJD2C/β-catenin signalling pathway

BackgroundRecurrence and metastasis are the main causes of disease deterioration in colorectal cancer (CRC) patients, yet efficient therapeutic strategies are lacking. Natural compounds for efficient antitumour therapeutics are becoming increasingly prominent. Kaempferol, one of the main components of flavonoids in plants, displays a variety of pharmacological activities. Our preliminary experiments suggested that kaempferol could inhibit CRC metastasis and is significantly associated with the β-catenin signalling pathway. Moreover, we also defined the regulatory roles of JMJD2C in β-catenin signalling in our previous work. PurposeThis study aims to reveal the mechanism by which kaempferol inhibits CRC progression and regulates the JMJD2C/β-catenin signalling pathway. MethodsThe migratory capabilities of CRC cells after kaempferol intervention were measured by scratch wound healing and transwell assays. Circ_0000345 knockdown CRC stable cell lines were generated by lentivirus infection. The possible mechanism of kaempferol on circ_0000345 was verified by molecular-protein docking and verification program cellular thermal shift assay (CETSA). A dual luciferase reporter gene assay was carried out for the targeting relationship among circ_0000345, miR-205-5p and JMJD2C. Fluorescence in situ hybridization (FISH) was performed to determine the expression of circ_0000345 in tumour tissues. A pulmonary metastatic model of CRC in vitro was built to assess the antimetastatic effect and mechanism of kaempferol in vivo. ResultsIn vitro, kaempferol inhibits the ability to migrate of CRC cells by reducing the activation of the JMJD2C/β-catenin signalling pathway. MiR-205-5p is a key bridge for kaempferol to inhibit the expression of JMJD2C. The function of miR-205-5p is impeded by circ_0000345, which shows higher expression levels in human metastatic CRC tissues than nonmetastatic CRC tissues, and its formation is regulated by the RNA-binding proteins HNRNPK and HNRNPL. Mechanistically, kaempferol physically interacts with HNRNPK and HNRNPL to suppress JMJD2C by downregulating the expression of circ_0000345. In vivo, kaempferol suppresses CRC lung metastasis. Kaempferol inhibits the activation of JMJD2C/β-catenin signalling through reducing the expression of circ_0000345 in the CRC lung metastasis model. ConclusionCirc_0000345 enhances activation of the JMJD2C/β-catenin signalling pathway through miR-205-5p to promote CRC metastasis. Kaempferol inhibits CRC metastasis through the circ_0000345-mediated JMJD2C/β-catenin signalling pathway, and this effect is influenced as a direct consequence of the binding of kaempferol with HNRNPK and HNRNPL. This provides promising therapeutic and/or adjuvant agents for advanced CRC and sheds light on the multifaceted role of phytomedicine in cancer.

PYTHON PROGRAM FOR STRUCTURE AND MOLECULAR FORMULA OF ORGANIC COMPOUNDS: AN ALTERNATIVE THEORETICAL APPROACH

A chemist always desires the automation of a chemistry laboratory as it reduces their workload by minimizing the time, energy, and effort. Automation when coupled with artificial intelligence is the need in today’s era as it gives more flexibility and power to the chemist. Using computer programs for a large number of such operations would further ease out these tasks as the desired objectives may be achieved with the click of a mouse. In this research article, the Python program has been used to arrive at the empirical and molecular formulas of some compounds. Python code has also been written for some compounds and their 2D and 3D structures drawn henceforth. With the introduction of Python language at the undergraduate level, this paper would allow chemistry students to make use of Python in organic chemistry as well. As a result, the chemistry students will be able to use the Python program for verification and classroom-based structure determination of various organic compounds. The students thus gain access and become equipped with computational skills that will be helpful in the modern world and can utilize the Python code as an alternative theoretical approach for these operations. While Python provides valuable computational tools, integrating thematic chemistry and new methodologies is crucial for advancing structural analysis. This approach enhances the depth and applicability of theoretical models beyond basic calculations. This paper is a perfect amalgamation of computer programming with organic chemistry and is an attempt to equip the chemists to do the above-mentioned operations theoretically from their desks.

Planes geometry verification program written in Python

In recent years, there has been significant progress in sensor and scanning technologies in the computer vision and 3D visualization field. This has led to an increased interest in processing and analysing point clouds, which are groups of 3D points collected by laser scanners, stereo cameras, and drones. The data obtained from these devices has a wide range of applications. This article focuses on using point cloud data to verify planar structural elements of buildings. The article aims to present a methodology for analysing planar point cloud objects using 3D models in IFC format as reference data. The research includes processing data in IFC format, exporting geometric information, segmenting plane objects from a point cloud obtained by laser scanning, and analysing the data obtained. We provide the necessary graphic materials and code to enhance the content of the article. We also evaluate the results and create an outline for future work.

Biocidal Verification Program for Healthcare Facilities: Evaluation of the Efficacy and Microbial Tolerance of Disinfectants

Context: The disinfection of healthcare facilities is of utmost importance to prevent the spread of infectious diseases. In this regard, biocidal activity testing is used to evaluate the efficacy of disinfectants in hospitals. Aim: Establishing a disinfectant efficacy test aimed at combating infections and cross-contamination. Settings and Design: The experiment was carried out in two treatment groups for each of the four microorganisms on ten surface coupons, with a viability control group determined as the baseline inoculum. Methods and Materials: The study used two disinfectants—hydroxyethane (ethylene hydrate) and 2-hydroxypropane (sec-propyl alcohol)—to evaluate their efficacy in reducing bacterial contamination on inanimate surfaces. Statistical analysis used: The data obtained was analyzed using Minitab software, which involved a normal probability plot, a homogeneity of variances test, and a test for equal variances. The statistical significance level was set at α = 0.05. Results: The normal probability plot of microorganism-disinfectant groups with a 95% confidence interval showed improvement in normalization after the logarithmic transformation of datasets. The homogeneity of variances test did not reveal any statistically significant differences between the groups, and all the comparison intervals overlapped for the logarithmically transformed datasets in contrast to the raw datasets. The bacterial reduction after exposure to disinfectants for five minutes compared with the control group in the surface challenge biocidal test expressed as absolute and logarithmic reduction showed a significant reduction in bacterial growth, with Burkholderia cepacia showing greater persistence on the examined sample of the surfaces. Conclusions: The current study showed that the effect of the microorganism tolerance contributed to the variation in responses rather than the disinfectant or the surface types, suggesting the risk of resistant bacteria on the contaminated surface in the dissemination of infections. Thus, monitoring and routine disinfection validation programs for the control of microbial burden in healthcare are crucial for infection and contamination mitigation

Microsatellite markers for genotyping of Kodamaea ohmeri: Demonstrating outbreaks based on a multicenter surveillance study in China

Kodamaea ohmeri, an emerging human pathogen, caused both sporadic and nosocomial infections among immunocompromised people with high mortality. However, there is limited research on the molecular epidemiology of K. ohmeri. A total of fifty microsatellite loci were designed based on K. ohmeri type strain NRRL Y-1932 and three loci were finally selected for microsatellite analysis. Non-duplicated K. ohmeri isolates and strains of other species were collected across China as a part of CHIF-NET program for sensitivity and specificity verification. Antifungal susceptibility was determined using Sensititre YeastOne TM YO10. The three loci (P10, P11 and P26), with a cumulative discriminatory power of 0.98, exhibited a prospective specificity and reproducibility in the PCR of 92 K. ohmeri strains from different hospitals. A total of 54 microsatellite types (MT) were identified and most of them distributed sporadically. However, six strains of MT12 clustered in HZ hospital and were isolated in the same department within two months, indicating a potential outbreak. Of seven isolates exhibited MIC values of >8 mg/L for fluconazole, three isolates from LR hospital shared the same genotype of MT44. Herein, we established a set of microsatellite loci for K. ohmeri, as a rapid and specific tool for genotyping K. ohmeri, and identified several potential clusters. This study will help us better understand the molecular epidemiology of the emerging pathogen K. ohmeri.

Ontological modeling of the psychological field of a play

Ontological modeling of the psychological field of a play

Assessing Food Safety Compliance in a Small-Scale Indian Food Manufacturer: Before and after Certification of the Food Safety Management System and Foreign Supplier Verification Program

The implementation of food safety management systems has a great influence on global food safety and security, especially for developing nations. The principles of the Hazard Analysis and Critical Control Points are crucial for food safety and are the basis of food safety management systems. The purpose of this study was to assess the food safety management systems of a selected small-sized food company in India, both before and after certification of the International Organization for Standardization (ISO) 22000 and the Foreign Supplier Verification Program (FSVP). This company manufactured traditional fried vegetable snacks using manual processing. The effectiveness of these certifications was assessed through direct audit. The integrated FSVP and ISO 22000 audit checklist were elaborated and applied in this study. It was shown that the certification significantly improved (97.14%) food safety system conformity in the company in question. Before certification, many non-compliances were found, especially for HACCP implementation. After certification, the only non-conformity concerned pest control. The integrated FSVP and ISO 22000 audit checklist used for the assessment enabled an evaluation of several factors: the food quality and safety compliance level, the effectiveness of certification, and the readiness of the small-sized Indian snack producer to develop the export of their products to the U.S. and other developed countries. It was shown that certification enabled a small-sized Indian food producer to meet the requirements of ISO 22000 and FSVP, which are the prerequisites for exporting to the U.S. and other developed countries.

Clinical pharmacist prescription review and modification for oral antineoplastic medications at an academic medical center.

64 Background: Collaboration between oncology providers and pharmacy teams has improved the safety of oral antineoplastic (OA) prescribing, but opportunities remain for improvements in care delivery. To this end, starting in March 1, 2021, the Dana Farber Cancer Institute Specialty Pharmacy implemented a new workflow - clinical verification of new specialty prescriptions by dedicated OA clinical pharmacists (OAPs) prior to prescription processing. To reduce the impact of initial prescription errors on efficiency and safety, one component of clinical verification was to expand the purview of OAPs to make certain modifications to prescriptions that could be automatically processed without requiring prescriber re-signing. Automatically processed prescription modifications include corrections to quantity or days supply, indication of cycle length or days on/off therapy, and clarification of directions. Here we report on the impact of this portion of the clinical verification program. Methods: OAP prescription modification (OAP-PM) was introduced on November 1, 2022. To assess the impact on efficiency, we conducted a pre-post analysis of the proportion of prescription modifications taking > 60 minutes to complete for 6 months before and after OAP-PM introduction. Using prescription-level data, we analyzed co-variates including pre-/post-OAP-PM, patient age (continuous), site (academic vs. network), communication with someone other than the primary prescriber (y/n), and medication category (anti-neoplastic vs. supportive). We used control charts to explore the contribution of specific medications and sites that might share best practices or be the focus of subsequent, more focused improvement projects. Results: From May 1, 2022 to May 1, 2023, 458 prescription modifications were completed, 180 pre- and 278 post-OAP-PM. The proportion of prescriptions requiring > 60 minutes to complete decreased from 29.4% to 19.8% (p = 0.0184). In multivariable regression, the significant predictors of prescription taking > 60 minutes to complete were pre-OAP-PM (LR 4.9, p = 0.027); network (LR 20.8, p < 0.0001); and communication with someone other than the primary provider (LR 18.6, p < 0.0010). Control charts demonstrated all sites were within 3-sigma limits pre- and post-OAP-PM, but with wide limits at many sites. Individual medications could not be meaningfully analyzed due to the small sample sizes. Conclusions: OAP-PM successfully reduced the proportion of OA prescriptions taking more than an hour to modify. This may be a useful workflow change for other specialty pharmacies to adopt to improve the efficiency of OA care. Additional work is needed to understand whether site-, provider- or medication-specific changes can improve upon this model.

Production and Performance Assessment of a Severe Acute Respiratory Syndrome Coronavirus 2 Biomimetic in a Verification Program for Pandemic Readiness

During the early stages of the 2019 coronavirus disease (COVID-19) pandemic in South Africa, one of many challenges included availability of control material for laboratory proficiency testing programs. Proficiency testing control material using live severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or RNA extracted from cell culture was either biohazardous or costly, particularly in resource-limited settings. This study reports the development and application of a noninfectious SARS-CoV-2 biomimetic Mycobacterium smegmatis strain that mimics a positive result in the GeneXpert SARS-CoV-2 Xpert Xpress cartridge. Nucleotide sequences located in genes encoding the RNA-dependent RNA polymerase, the nucleocapsid, and the envelope proteins were used. The resulting biomimetic strain was prepared as a positive proficiency testing control and distributed in South Africa for verification of laboratories before their testing of clinical specimens. Between April and December 2020, a total of 151 GeneXpert instruments with 2532 modules were verified to bring COVID-19 mass testing in South Africa online. An average concordance of 98.6% was noted in the entire laboratory network, allowing identification of false-positive/false-negative results and instrument errors. This noninfectious, easily scalable proficiency testing control material became available within 2 months after the start of the pandemic in South Africa and represents a useful approach to consider for other diseases and future pandemics.

Creation of Equipment for Testing Roofs for Resistance to External Fire Influence

This paper examines and analyzes the domestic and foreign regulatory framework, guidelines and technical documents, information brochures, manuals, handbooks, test reports and other literary sources as well as information on the Internet. In the countries of the European space, fire safety requirements have been established for roofs and roofing materials, both for roofing materials and for roofs as part of structures, which helps to limit the spread of fire in buildings and structures during a fire. It has been proven that there is a need to create equipment for testing resistance to external fire exposure in accordance with DSTU CEN/TS 1187:2016 (method 2) of coverings of buildings (roofs) and establishing fire classification according to DSTU EN 13501-5:2016. A registration certificate, a technical description, an operating manual, and a program and methodology of the test bench verification have been developed. The initial verification of the test bench was carried out and it was put into operation. Approbation of the test method was carried out on the created test bench through experimental studies of five types of roofing materials being widely used on construction works in Ukraine using two bases (substrates) of combustible and non-combustible materials at air flow speeds of 2 m/s and 4 m/s above the specimen surface. According to the results of this work, it was established that the maximum relative deviation of the result of measuring the length of the damaged (charred) part of the specimens was in the range of 1.2 % to 23.3 % for the roof and 0.7 % to 27.5 % for the base.

Plasma-Sprayed Coatings Resist Erosion in Wire-Wrapped Screens

_ This article, written by JPT Technology Editor Chris Carpenter, contains highlights of paper IPTC 21158, “Development of Plasma-Sprayed Coatings To Improve the Erosion Resistance of Wire-Wrapped Screens,” by Lukas Ochmann, SPE, and Mohd M. Amro, SPE, TU Bergakademie Freiberg, and Bernd Gronde, Fraunhofer IKTS Hermsdorf, et al. The paper has not been peer reviewed. Copyright 2021 International Petroleum Technology Conference. Reproduced by permission. _ A newly developed coating is put on the outside (i.e., facing the formation) of standard wire-wrapped screens to make the slots erosion-resistant. The coating consists of ceramic or hard metal and is applied by plasma spraying. An extensive development and verification program was conducted to guarantee defined slot widths, corrosion resistance, and mechanical strength of coating and screen. The coating improves resistance against erosion, can withstand corrosive well environments, and features mechanical properties suitable to be safely installed in any well. Thermal Spraying and Composition of Coatings The screens are coated using thermal spraying. This process uses a hot plasma jet to melt powdery materials and apply them to a solid surface. The molten particles solidify on the surface and form a hard coating with little porosity. No chemical reaction exists between the base material and the coating. Several different steps are necessary to coat a wire-wrapped screen. First, the screen is sandblasted in a distinct way to provide a defined surface roughness. After that, a thin bonding layer is applied by thermal spraying. This intermediate layer further improves the adhesion of the functional layer. This erosion-resistant main layer, which consists of hard metal or ceramic, is applied next by thermal spraying. The coating is finished by filling up the pores of the sprayed layers with a liquid sealer. The coating changes the geometry of the slots, slightly reducing the slot size and creating a less-sharp slot opening. By adjustment of the process parameters of the thermal spraying and choice of the correct initial slot width, defined and constant slot widths can be produced for the final product. Corrosion Investigation All samples and future prototypes are based on 1.4404 (316L) stainless steel. Combinations of four bonding layers, six functional layers, and five sealers were investigated. In total, 237 different samples were corroded under different conditions. The samples were submerged halfway into the brine so that the effect of the three-phase interface (brine, gas, and sample) could be investigated over a period of multiple weeks (minimum 3 weeks, maximum 18 weeks) at 60°C. Further experiments were conducted with nitrogen and hydrogen atmospheres for 3 weeks. The first tests were conducted with samples made from wire-wrapped screens. A second batch of tests was performed with coupons for tensile bond tests.

Development of the Independent Dose Verification Method for the Ring Gantry PET/CT Linac

The RefleXion Xi is a new external beam radiotherapy delivery modality combining kilovoltage fan-beam CT and on-board PET for Biology-guided Radiotherapy. Although the machine shares similar components with the Tomotherapy machine, it has unique hardware features such as stationary beam delivery system with couch increments. The goal of this study is to develop an in-house independent secondary dose calculation method for this ring gantry PET/CT Linac. The method aggregates the beam intensities at each discrete firing gantry angle and couch moving position. The non-uniform intensity map is decomposed into a series of segments of uniform beam intensities, and then coordinates of beam segments were modified based on the relative distance from the dose calculation point to keep the calculation point in the same spot along the gantry rotation. The dose to the calculation point of each segment is determined by using measured tissue-maximum-ratio, output factors, and off-axis ratio by independent binary collimators. Two-dimensional convolution method is applied to integrate all dose contribution components in segment areas for efficient calculation. The final dose is obtained by summing all dose to the calculation point of segments for all firing gantry angle and couch position. Twenty patients with different treatment sites including head and neck, prostate, and lung regions were analyzed. Comparison of the point dose calculated by the independent program to that calculated by the planning system has shown reasonable agreement within ±5%. The independent dose verification program has been developed as an initial patient specific QA for improving patient safety. Further study will be performed to enhance the accuracy and reliability by including additional leakage and scatter models.

Evaluating potential disparities in geospatial access to American College of Surgeons/American Association for the Surgery of Trauma-verified emergency general surgery centers.

The American Association for the Surgery of Trauma and the American College of Surgeons have recently introduced emergency general surgery (EGS) center verification, which could enhance patient outcomes. Distance and resource availability may affect access to these centers, which has been linked to higher mortality. Although many patients can receive adequate care at community centers, those with critical conditions may require specialized treatment at EGS-verified centers. We aimed to evaluate geospatial access to potential EGS-verified centers and identify disparities across different scenarios of EGS verification program uptake in the United States. We used hospital capabilities and verified pilot centers to estimate potential patterns of which centers would become EGS verified under four scenarios (EGS centers, high-volume EGS centers, high-volume EGS plus level 1 trauma centers, and quaternary referral centers). We calculated the spatial accessibility index using an enhanced two-step floating catchment technique to determine geospatial access for each scenario. We also evaluated social determinants of health across geospatial access using the Area Deprivation Index (ADI). A total of 1,932 hospitals were categorized as EGS centers, 307 as high-volume EGS centers, 401 as high-volume EGS plus level 1trauma centers, and 146 as quaternary centers. Spatial accessibility index decreased as the stringency of EGS verification increased in each scenario (226.6 [111.7-330.7], 51.8 [0-126.1], 71.52 [3.34-164.56], 6.2 [0-62.2]; p < 0.001). Within each scenario, spatial accessibility index also declined as the ADI quartile increased ( p < 0.001). The high-volume EGS plus level 1trauma center scenario had the most significant disparity in access between the first and fourth ADI quartiles (-54.68). Access to EGS-verified centers may vary considerably based on the program's implementation. Disadvantaged communities may be disproportionately affected by limited access. Further work to study regional needs can allow a strategic implementation of the EGS verification program to optimize outcomes while minimizing disparities. Prognostic and Epidemiological; Level IV.

Quantifying additionality thresholds for forest carbon offsets in Mississippi pine pulpwood markets

Concerns over the additionality of carbon sequestration achieved through voluntary carbon market have threatened offset market credibility and stability. There is an urgent need to examine additionality in a dynamic market context. To this end, our analysis focuses on the extent to which deferred harvests of pine roundwood as an offset source can achieve additionality under changing roundwood prices across a large wood-producing region. Specifically, we consider the potential for offset market activity itself and for unexpected demand shocks to adjust carbon baselines as they each have an impact on the relative prices observed for industrial roundwood. We use a bioeconomic model of the roundwood market to simulate harvest activity and estimate the economically feasible levels of carbon storage across four wood-producing basins in Mississippi. We then present estimates of the expected changes in carbon storage under a given change in timber prices and demonstrate how this information can be used to approximate the optimal reaction curve for an offset broker, dealer, or verification program manager seeking to ensure additionality with credits they exchange. The optimal reaction curve consists of a response to a change in the roundwood price state by either approving supplementary harvest deferral contracts or by restricting the supply of new contracts, depending on the direction of the observed price change. Alternatively, the results suggest that contracts could be structured to facilitate payment conditional on realized timber price outcomes, rather than solely on evidence of a delayed harvest.

Innovative approaches in the management of an educational institution

The purpose of this study is to investigate the possibility of using digital and other types of innovations in the management of educational institutions in the country. The methods of content analysis of documents and scientific papers, institutional, system-structural approaches, and other means of scientific and theoretical analysis were used in the study. The investigation of the main areas of improving the management of educational organisations in Kazakhstan, various management decisions and attempts to implement state programme measures endows this study with socioeconomic and applied research character. This study shows the shortcomings of the management plan on the development of school curricula for mathematics, natural sciences, and humanities using the results of representatives (schoolchildren) of educational organisations of Kazakhstan passing the international assessment and programme verification (Programme for International Student Assessment, Programme for the International Assessment of Adult Competencies, and Trends in Mathematics and Science Study). The issues of the importance of advanced training of teachers of higher educational institutions, the reform of the entire education system, the problems of technical and technological provision of educational institutions in rural areas of the Turkestan region, digitalisation of the entire learning process were raised. The issues of material and financial security of educational activities in the region and the development of an inclusive education system were also considered. Based on the research of foreign researchers, the peculiarities of the introduction of innovations in education and the informatisation of education were considered.

Emergency general surgery verification: Quality improvement and the case for optimal resources and process standards.

Patients with emergency general surgery (EGS) diagnoses comprise over 10% of all hospital admissions, resulting in a projected number of 4.2 million admissions for 2023. Approximately 25% will require emergency surgical intervention, half will sustain a postoperative complication, and 15% will have a readmission within the first 30 days of surgery. In the face of this growing public health burden and to better meet the needs of these acutely ill patients, it was recognized that a formal quality improvement program, including standardization of data collection and the development of systems of care specifically for EGS have been lacking. Establishing standardized processes for quality improvement, including a national databank, and maintaining adherence to these processes as ensured by a robust verification process has improved outcomes research and patient care in the field of trauma, another time-sensitive specialty. In response to this perceived deficit, the "Optimal Resources for Emergency General Surgery" was developed. An extension of the current National Surgical Quality Improvement Program platform, specifically for operative and non-operative EGS cases, was developed and implemented. A robust set of standards were outlined to verify EGS programs/services. Defining the elements of an effective EGS program and developing hospital and practice standards consolidated EGS as an integral component of Acute Care Surgery. The verification program addresses a societal need and allows hospitals to better organize EGS care delivery and benchmark their results nationally.

Preoperative Code Status Discussion Workflows: Targets for Improvement in Multidisciplinary Pathways.

Discussion of perioperative code status is an important element of preoperative care and a component of the American College of Surgeons' Geriatric Surgery Verification (GSV) program. Evidence suggests code status discussions (CSDs) are not routinely performed and are inconsistently documented. Because preoperative decision making is a complex process spanning multiple providers, this study aims to utilize process mapping to highlight challenges associated with CSDs and inform efforts to improve workflows and implement elements of the GSV program. Using process mapping, we detailed workflows relating to (CSDs) for patients undergoing thoracic surgery and a possible workflow for implementing GSV standards for goals and decision-making. We generated process maps for outpatient and day-of-surgery workflows relating to CSDs. In addition, we generated a process map for a potential workflow to address limitations and integrate GSV Standards for Goals and Decision Making. Process mapping highlighted challenges associated with the implementation of multidisciplinary care pathways and indicated a need for centralization and consolidation of perioperative code status documentation.

챗(Chat) GPT의 이용과 저작권 쟁점 고찰

The development of big data analysis and deep learning technologies triggered by the 4th industrial revolution created an opportunity to expand the scope of application and application of artificial intelligence to our lives. Beyond the level where conventional artificial intelligence was merely a means of assisting human decision-making or action, it has reached a stage where it analyzes vast amounts of data to identify problems on its own, create optimal answers, and even self-examine errors. A representative example is the Chat GPT service. Recently, however, copyright infringement disputes have been reported in this regard. For example, in January 2023, Getty Images filed a copyright infringement lawsuit against Stability AI, the developer of 'Stable Diffusion', and in April 2023, Twitter CEO Elon Musk also announced its intention to file a copyright infringement lawsuit against Microsoft (MS). In this regard, in this paper, first, as an issue derived from data use, chat GPT does not disclose how to learn data, so it is difficult to confirm whether scrolling and scraping are prohibited information has been collected, and chat GPT service is fair. It was pointed out that there are elements that are difficult to see as use, that the scope of TDM exemption is different for each country, and that TDM exemption regulations have not yet been introduced in Korea. Then, as a way to determine and protect copyright infringement of chat GPT products, first, in relation to authors and rights attribution, the contribution of artificial intelligence and humans to creation is divided and marked by stages (by year), and rights attribution must be different accordingly, second, in relation to the judgment of substantial similarity, refer to the criteria for functional works, but in the case of artificial intelligence products, there is a limit to determining whether or not copyright infringement is caused only by human feelings, so plagiarism verification or copyright infringement prevention programs must be developed and applied. Third, in relation to copyright protection institutions, it is appropriate to stipulate the protection period of artificial intelligence products at 5 years, but a two-dimensional approach must be taken so as not to reduce the incentive for human creation. Fourth, in relation to civil and criminal legal responsibilities, the Product Liability Act Referring to the provisions of the Senate Civil Act, it was proposed that artificial intelligence developers and users jointly and jointly take responsibility in principle, but that responsibilities should be assigned differentially depending on whether there was a violation of the duty of care or negligence. Based on the contents examined in this paper, it is necessary to actively seek rational and systematic ways to regulate generative artificial intelligence services.

First Evidence of Multi-iron Subpopulations in the Bulge Fossil Fragment Candidate Liller 1* * Based on observations collected at the Very Large Telescope of the European Southern Observatory, Cerro Paranal (Chile), under the ESO-VLT Multi-Instrument Kinematic Survey (MIKiS survey) programs 106.21N5 and 105.20B9 (PI: Ferraro) and under MUSE science verification programs: 60.A-9489;60.A-9343.

In the context of a project aimed at characterizing the properties of the so-called Bulge Fossil Fragments (the fossil remnants of the bulge formation epoch), here we present the first determination of the metallicity distribution of Liller 1. For a sample of 64 individual member stars we used ESO-MUSE spectra to measure the equivalent width of the Ca II triplet and then derive the iron abundance. To test the validity of the adopted calibration in the metal-rich regime, the procedure was first applied to three reference bulge globular clusters (NGC 6569, NGC 6440, and NGC 6528). In all the three cases, we found single-component iron distributions, with abundance values fully in agreement with those reported in the literature. The application of the same methodology to Liller 1 yielded, instead, a clear bimodal iron distribution, with a subsolar component at [Fe/H] = −0.48 dex (σ = 0.22) and a supersolar component at [Fe/H] = +0.26 dex (σ = 0.17). The latter is found to be significantly more centrally concentrated than the metal-poor population, as expected in a self-enrichment scenario and in agreement with that found in another bulge system, Terzan 5. The obtained metallicity distribution is astonishingly similar to that predicted by the reconstructed star formation history of Liller 1, which is characterized by three main bursts and a low, but constant, activity of star formation over the entire lifetime. These findings provide further support to the possibility that, similar to Terzan 5, Liller 1 is also a Bulge Fossil Fragment.

Shared Decision Making in the Geriatric Surgery Verification Program: Assessing Baseline Performance.

As part of the launch of the Geriatric Surgery Verification program in 2019, the American College of Surgeons issued care standards for older patients, including requirements for preoperative documentation of patients' goals. Hospital performance on these standards prior to the Geriatric Surgery Verification program is unknown. To assess baseline performance of the Geriatric Surgery Verification (GSV) standard for documentation of preoperative goals for older patients, and to determine factors associated with standard adherence. Using natural language processing, this study examines the electronic health records of patients aged 65 years or older who underwent coronary artery bypass grafts (CABG) or colectomies in 2017 or 2018 at three hospitals. The primary outcome was adherence to at least one of the three components of GSV Standard 5.1, which requires preoperative documentation of overall health goals, treatment goals, and patient-centered outcomes. A total of 2630 operations and 2563 patients were included. At least one component of the standard was met in 307 (11.7%) operations and all three components were met in 5 (0.2%). Higher likelihood of meeting the standard was demonstrated for patients who were female (odds ratio [OR] 1.30; 95% CI 1.00-1.68), undergoing colectomy (OR 2.82; 95% CI 2.15-3.72), or with more comorbidities (Charlson scores >3 [OR 1.55; 95% CI 1.14-2.09]). Before GSV program implementation, clinicians for two major operations almost never met the GSV standard for preoperative discussion of patient goals. Interdisciplinary teams will need to adjust clinical practice to meet best-practice communication standards for older patients.