Venda Region Research Articles

Prevalence of Campylobacter species, Helicobacter pylori and Arcobacter species in stool samples from the Venda region, Limpopo, South Africa: Studies using molecular diagnostic methods

This study determined the prevalence of Campylobacter spp., Helicobacter pylori and Arcobacter spp. in stool samples from Venda in relation to diarrhea, intestinal inflammation and HIV status using specific molecular methods. Stool samples were collected from hospital patients (255) and primary school children (67). Genomic DNA was extracted from the stools and molecular methods including PCR, PCR followed by restriction analysis and multiplex PCR were used to test for the different organisms. The lactoferrin content of the stools was determined using commercial kits from TechLab (Blacksburg, VA, USA). The prevalence of the different organisms was 50.6% for H. pylori, 10.2% for C. jejuni, 6.2% for A. butzleri, 6.5% for C. coli, 3.1% for C. concisus, 2.8% for A. cryaerophilus and 1.9% for A. skirrowii. Of all the organisms, only C. jejuni was significantly associated with diarrhea (84.8%) (chi2=21.025, P<0.001) and elevated levels of lactoferrin (78.8%) (chi2=16.919, P<0.005) and was an important pathogen associated with diarrhea among HIV positive individuals (22.8%). Campylobacter infections are common causes of gastroenteritis in Venda. Non-C. jejuni/coli Campylobacters such as C. concisus as well as A. butzleri and H. pylori may be involved in gastrointestinal diseases in the region but further studies are needed to confirm this hypothesis.

PREVALENCE AND SPECIES DISTRIBUTION OF E. HISTOLYTICA AND E. DISPAR IN THE VENDA REGION, LIMPOPO, SOUTH AFRICA

The prevalence and species distribution of Entamoeba histolytica and E. dispar in the Venda region were determined in stool samples collected from public hospitals and primary schools by ELISA and a nested polymerase chain reaction (PCR). E. histolytica was detected in 37/197 (18.8%) and 1/47 (2.1%) samples, whereas 50/197 (25.3%) and 4/47 (8.5%) had E. dispar in the hospitals and schools, respectively. The age groups most infected were 0-2 (33%) years followed by 20-29 years (27%). E. histolytica was significantly associated with diarrhea (77.4% versus 22.6%; chi 2 = 39.48, P < 0.05), and with the presence of lactoferrin (85.7% versus 14.2%) in the stools, indicating intestinal inflammation (chi 2 = 29.605, P < 0.05). E. histolytica was found in 5 (16.12%) of the 31 HIV-positive individuals and in 33 (15.5%) of the 213 HIV-negative individuals. E. histolytica infections are common in the Venda region and are associated with diarrhea and intestinal inflammation.

Cryptosporidium species: Preliminary descriptions of the prevalence and genotype distribution among school children and hospital patients in the Venda region, Limpopo Province, South Africa

In the present study, the prevalence and species distribution of Cryptosporidium among school children and hospital patients in the Venda region of South Africa was determined. Real time PCR (qPCR) was used for initial screening to detect positive samples while a nested PCR followed by restriction fragment length polymorphism was used to determine the species genotype. From a total of 244 stool samples tested, 44 (18%) had Cryptosporidium with no significant difference ( χ 2 = 0.04; P = 0.841) between samples collected from patients attending hospitals 36/197 (18%) and the samples from primary schools 8/47 (17%). The age groups most affected were those from 2 to 5 years old (28.6%) and 50 to 59 years old (50.0%). Cryptosporidium was detected in 4 (12.5%) of the 31 HIV positive individuals. Fifty-seven percent of the Cryptosporidium positive samples were diarrheic and 26 (59.1%) had elevated lactoferrin content. C. hominis (82%) was more common than C. parvum (18%). This study has demonstrated the high prevalence of Cryptosporidium infections in the Venda region and its implications in causing diarrhea and inflammation.

Cost–benefit analysis of a disease control programme with special reference to ticks and tick-borne diseases in the former Venda region

This study is based on a cross-sectional survey of 125 small-scale cattle farmers interviewed in the Venda region of the Limpopo Province of South Africa. It revealed a 3 per cent mortality rate in spite of the existence of a dipping programme. Cost–benefit analysis revealed a cost–benefit ratio of 0.8 (i.e. <1) indicating that the control of ticks and tick-borne diseases by the government is not economically justified. However, because of the broader socio-economic benefits it provides, the dipping of cattle still deserves governmental support. In addition, the provision of tick control services by the government leads to a socially optimal level of supply of animal health services in general. Sensitivity analysis gives a cost–benefit ratio of 1.2 when it is assumed that the mortality rate would have been 10 per cent without the control programme.

Activity profiles of fourteen selected medicinal plants from Rural Venda communities in South Africa against fifteen clinical bacterial species

Fourteen plants used in traditional medicine in the Venda region of South Africa were screened for activity against fifteen bacterial species. Methanol, acetone and hexane extracts and in some cases essential oils were tested using the disc diffusion and the microdilution methods. Most of the extracts were active against at least one bacterial species. Methanol and acetone extracts were the most active while Gram positive bacteria were the most sensitive as compared to Gram negative bacteria. This study has revealed the strong in vitro activity of Syzigium cordatum, Peltophorum africanum, Rhoicissus tridentata, Bridelia micrantha and Ximenia caffra against Gram positive and Gram negative bacteria. Essential oils of Lippia javanica was also effective against most of the bacterial species studied. However, Pouzolzia mixta and Mucuna coriaceae showed less activity. Some plants were more active than commercial antibiotics. This study is the first to test the activity of the selected plants from the Venda region against such number of bacterial isolates and justifies their use by local traditional healers. The identification of the active components of the plants and the determination of the effect of these plants on the immune system will give more information on their activity. Finally, these results may be of importance in identifying candidate plants and essential oils for eventual drug design and other therapeutic purposes, respectively.

Bacterial contamination of Vhuswa--a local weaning food and stored drinking-water in impoverished households in the Venda region of South Africa.

Bacterial contaminants of Vhuswa--a traditional maize-based weaning food, and domestic drinking-water stored in impoverished rural households in Venda of Limpopo province, South Africa, were determined. One hundred and twenty-five samples of Vhuswa fed to children aged less than five years were assessed for Escherichia coli, Campylobacter jejuni, Salmonella, and Shigella. The microbiological quality of 125 drinking-water samples was also evaluated using total coliforms, faecal coliforms, and faecal streptococci as indicators. The frequency of isolation of E. coli, Salmonella, Shigella, and C. jejuni from the Vhuswa samples was 70%, 5%, 5%, and 2% respectively. The geometric mean counts of total coliforms, faecal coliforms, and faecal streptococci in tap-water stored in household containers ranged from 4.9x10(2) to 5.8x10(3) cfu 100 mL(-1), 2.6x10(2) to 3.7x10(3) cfu 100 mL(-1), and 3.1x10(3) to 5.8x10(3) cfu 100 mL(-1) respectively, and for stored spring water it was 5.1x10(3) cfu 100 mL(-1), 3.2x10(3) cfu 100 mL(-1), and 5.1x10(3) cfu 100 mL(-1) respectively. The frequent contamination of water and food samples in this study has important implications for the health of children from impoverished communities.

Activity profiles of fourteen selected medicinal plants from Rural Venda communities in South Africa against fifteen clinical bacterial species

Fourteen plants used in traditional medicine in the Venda region of South Africa were screened for activity against fifteen bacterial species. Methanol, acetone and hexane extracts and in some cases essential oils were tested using the disc diffusion and the microdilution methods. Most of the extracts were active against at least one bacterial species. Methanol and acetone extracts were the most active while Gram positive bacteria were the most sensitive as compared to Gram negative bacteria. This study has revealed the strong in vitro activity of Syzigium cordatum, Peltophorum africanum, Rhoicissus tridentata, Bridelia micrantha and Ximenia caffra against Gram positive and Gram negative bacteria. Essential oils of Lippia javanica was also effective against most of the bacterial species studied. However, Pouzolzia mixta and Mucuna coriaceae showed less activity. Some plants were more active than commercial antibiotics. This study is the first to test the activity of the selected plants from the Venda region against such number of bacterial isolates and justifies their use by local traditional healers. The identification of the active components of the plants and the determination of the effect of these plants on the immune system will give more information on their activity. Finally, these results may be of importance in identifying candidate plants and essential oils for eventual drug design and other therapeutic purposes, respectively.

Profiles of antibiotic susceptibilities of bacterial isolates and physico-chemical quality of water supply in rural Venda communities, South Africa

The Venda region of South Africa is predominantly rural and residents rely on untreated water sources for daily water needs. The physico-chemical quality of these water sources including antibiotic susceptibilities of enteric bacterial isolates which would guide clinicians in the empiric management of diarrhoea have received cursory attention. This study therefore sought to document the physico-chemical parameters and antibiograms of bacterial isolates from water sources in order to determine the safety for human consumption and to provide updated antibiotic data for empiric treatment of patients. Water samples were obtained on a weekly basis from Ngwedi, Mutale, Tshinane, Mutshindudi and Mudaswali Rivers and Makonde, Mudaswali and Thamathama Fountains between August 2000 and July 2002. Physico-chemical parameters such as turbidity, temperature, pH, lead, fluoride, cyanide, iron, sulphate, chromate and nitrate were determined. Antibiograms of bacterial isolates were ascertained using the disk diffusion method. Results obtained revealed that all the physico-chemical variables of the water sources analysed were within normal recommended limits for safety of drinking water except for turbidity, which exceeded recommended limits and hence precludes the rivers from direct domestic use. Antibiogram profiles showed multiple antibiotic resistances of Salmonella, Shigella, Campylobacter, Aeromonas, Vibrio cholera, Enterobacter and Plesiomonas to ampicillin, tetracycline, chloramphenicol, cotrimoxazole and erythromycin. In contrast virtually all the enteric bacterial isolates showed marked susceptibilities to ciprofloxacin, nalidixic acid, gentamicin, ceftriazone and amikacin. These effective antibiotics are therefore indicated in the empiric treatment of diarrhoeal cases or water-borne diseases of bacterial aetiology. Keywords: Water quality, Microbial, Physico-chemical, Antibiograms, Enteric bacteria, Rural communities, Venda region WaterSA Vol.30(4) 2004: 515-519

Gene encoding virulence markers among &lt;i&gt;Escherichia coli&lt;/i&gt; isolates from diarrhoeic stool samples and river sources in rural Venda communities of South Africa

River water sources and diarrhoeic stools of residents in the Venda Region, Limpopo Province of South Africa were analysed for the prevalence of Escherichia coli (E. coli) and the presence of virulence genes among the isolates. A control group of 100 nondiarrhoeic stool samples was included. Escherichia coli was isolated and identified by standard cultural and biochemical methods. Pathogenicity of environmental and human isolates was determined by amplification of genes associated with virulence of E. coli, using specific primers. Of a total of 228 water and river sediment samples screened, E. coli was recovered from 200 (87.7%), and 135 (67.5%) of these had one or more genes associated with pathogenicity. The highest frequency of isolation of pathogenic strains was found in Ritavi River water and sediment (80.6%), followed by Lotanyanda River (76.9%), and the least (45.8%) in Nzhelele River 2. Escherichia coli was recovered from all of the 252 diarrhoeic stools tested (100%), and 119 (47.28%) of these had one or more genes associated with pathogenicity. The frequency of isolation of potential pathogenic E. coli from humans was highly significant (t = 6.3; pd&#3618;•0.01) in comparison to water isolates. Cytotoxic necrotizing Factor 1 (cnf1) and cytotoxic necrotising Factor 2 (cnf2) coding for necrotoxigenic E. coli (NEC); bundle-forming pilus (bfpA) and enteropathogenic attachment and effacement (eaeA) coding for enteropathogenic E. coli (EPEC), occurred in 35% and 34% respectively of river isolates. Heat-stable (ST) and heat-labile (LT) toxin genes coding for enterotoxigenic (ETEC) and Shiga-like toxin 1 (Stx1) and Shiga-like toxin 2 (Stx2) coding for Shiga-like toxin-producing E. coli (STEC) were not encountered in the river isolates. Isolates from stool samples had 21.8% and 12.6% of EPEC and NEC strains respectively; while enterotoxigenic (ETEC), Shiga-like toxin-producing (STEC) and enteroaggregative E. coli (EAEC) had a prevalence of 5%, 5.8% and 5.8% respectively. One human isolate possessed stx2 and eaeA indicating E. coliO157: H7. No genes associated with pathogenicity were observed in human non-diarrhoeic stool isolates. Results have revealed a possibility of a recycling of pathogenic E. coli strains, particularly the EPEC and NEC strains, between the water sources and the local population. Water SA Vol.30(1): 37-42

An economic assessment of the value of cattle to the rural communities in the former Venda region

This article assesses the economic value of livestock to rural communities in quantitative terms. A quantitative valuation of livestock is assessed in terms of milk production, manure use, draught power and offtake/sales criteria. The study is based on a cross-sectional survey of 125 small-scale cattle farmers interviewed in the Venda region of the Limpopo Province of South Africa. The results of the study estimated the average total value of an adult cow to be R1 152. This value should be viewed as an opportunity cost of the cattle. A quantitative valuation of livestock is important in indicating the degree of impact of cattle mortalities on the livelihood of rural households. In addition, it offers important guidelines for farmers' compensation by the government, should a disaster occur.

Scope of potential bacterial agents of diarrhoea and microbial assessment of quality of river water sources in rural Venda communities in South Africa

The microbial quality of several, usually untreated, surface domestic water sources, used by rural communities in the Venda Region of South Africa, was assessed to gauge their fitness for human consumption and to highlight the possible impact of waterborne diseases. The water sources studied were six points on the Levubu River and the rivers Mutale, Ngwedi, Tshinane, Makonde, Mutshindudi and Mudaswali. Total and faecal coliform, heterotrophic bacteria, enterococci and coliphage counts were used as indicators/surrogates to estimate the degree of bacterial and viral contamination respectively by standard methods. The presence of potential bacterial agents of diarrhoea such as Salmonella, Shigella, Campylobacter, Plesiomonas, Aeromonas and Vibrio was also determined. Results showed that the ranges of counts with regard to all the water sources investigated were 2.9 x 10(2) - 6.3 x 10(4) CFU/100 mL for faecal coliforms, 6.0 x 10(2) - 3.7 x 10(4) CFU/100 mL for total coliforms, 1.8 x 10(2) - 1.3 x 10(6) CFU/mL for heterotrophic plate count, 1.0 x 10(1) - 3.7 x 10(4) CFU/100 mL for enterococci and 0-13 PFU/100 mL for coliphages. These values are far higher than the acceptable maximum limits prescribed for South Africa by the Dept of Water & Forestry and the Water Research Commission - 0 CFU/100 mL, 5 CFU/100 mL, 1.0 x 10(2) CFU/mL, 0 CFU/100 mL and 1 PFU/100 mL for faecal coliforms, total coliforms, heterotrophic bacteria, enterococci and coliphages respectively. Salmonella, Shigella, Vibrio cholerae, Campylobacter, Aeromonas and Plesiomonas were isolated from several of the water sources investigated. The use of these water sources for drinking and domestic purposes poses a serious threat to the health and well being of the users and calls for urgent government intervention.

Assessment of the microbial quality of river water sources in rural Venda communities in South Africa

The microbial quality of several, untreated surface water sources, used by rural communities in the Venda region of South Africa, was assessed to determine its safety for human consumption and to highlight the possible occurrence of water-borne diseases. The water sources studied were six sampling points on the Levubu River, Vuwani, Mutale, Ngwedi, Tshinane, Makonde, Mutshindudi and Mudaswali Rivers. Heterotrophic plate counts, faecal and total coliforms, enterococci and somatic coliphage counts were performed according to standard methods to determine the microbiological quality of the water sources. The presence of enteric pathogens such as Salmonella, Shigella, Campylobacter, Plesiomonas, Aeromonas and Vibrio was also determined. Results obtained showed that the minimum and maximum counts with regard to all the sampling points investigated were 1.5 x 103 cfu·ml-1 and 6.3 x 104 cfu·ml-1 for faecal coliforms, 6.0 x 102 cfu·ml-1 and 3.7 x 104 cfu·ml-1 for total coliforms, 1.8 x 102cfu·ml-1 and 1.3 x 106 cfu·ml-1 for heterotrophic plate counts, 1.0 x 101 cfu·ml-1 and 2.5. x 104 cfu·ml-1 for enterococci and 0 and 13 pfu·100 ml-1 for somatic coliphages. The results for the indicators were higher than the acceptable maximum limits prescribed by the Department of Water and Forestry of South Africa. According to these guidelines, the maximum values are as follows: 0 cfu·100 ml-1 for faecal coliforms, 5 cfu·100 ml-1 for total coliforms, 1.0 x 102 cfu·ml-1 for heterotrophic plate count, 0 cfu·ml-1 for enterococci and 1 pfu·100 ml-1 for somatic coliphages. Salmonella, Shigella, Vibro, Campylobacter, Aeromonas and Plesiomonasspecies were isolated from several of the water sources investigated. These untreated water sources are used for drinking and domestic purposes and pose a serious threat to the health of the consumers and therefore calls for urgent intervention by government. WaterSA Vol.28(3) 2002: 287-292

A quantitative determination of the food security status of rural farming households in the Northern Province of South Africa

The majority of South Africans living in rural areas are food insecure despite high levels of national food self-sufficiency. The household food security position of two groups of rural farming households in the Venda region was evaluated quantitatively: one group produces vegetables that are sold or consumed locally, the other group produces cash crops – mangoes and other subtropical fruits. Using the collected data, food availability and energy, protein and fat requirements were calculated and balances derived. It was found that more than 80 per cent of the households had a negative balance of energy, protein and fat intake. The average energy, protein and fat coverage consumption was the same for both groups of farmers. A number of agricultural determinants were tested, but only non-agricultural determinants were found to be significant. These include household size, household (food) expenditure and proportion of the budget spent on food.

DEMAND FOR LIVESTOCK TICK CONTROL SERVICE IN THE VENDA REGION, NORTHERN PROVINCE

This article analyses factors influencing farmers' willingness-to-pay for dipping services, as well as the revealed preference for dipping frequency using the multivariate and the logistic regression models. The study is based on a cross sectional survey of 125 small-scale cattle farmers interviewed in the Venda region of the Northern Province. Empirical multivariate and the logistic regression analysis show that liquidity, human resource, satisfaction with the programme and structure of production significantly influences farmers' willingness-to-pay. However, the most important factor influencing both the willingness-to-pay and the dipping frequency is liquidity (employment). The results of this study have important implications for the delivery of veterinary services particularly in developing regions.

BUSINESS ORIENTATION AND THE FOOD SECURITY STATUS OF SMALL SCALE PRODUCERS IN THE VENDA REGION, SOUTH AFRICA

Questions addressed in this paper are: How to determine the food status of rural households; and does an agribusiness orientation enhance the food security status of farm families. A study was conducted on two groups of small scale black farmers in the Venda region: one group, agribusiness directed and generally more progressive towards technology, produces mangos and other subtropical fruits (cash crop farmers); the other group concentrates on locally consumed and sold vegetables (food crop farmers). Their food status was quantitatively determined by means of household energy, protein and fat balances and food security determinants were identified. More than 80% of the households had a negative balance for energy, protein or fat. Business orientation and farming type in this particular case study does not influence the energy, protein and fat coverage significantly. The marginal nature of farming by these small holders could explain this finding. Significant predictors of food security status were rather factors such as the household size and the availability of outside sources of income and cash to spend on food.

FINANCIAL ANALYSIS OF MANGO PRODUCTION ON SMALL-SCALE EMERGING COMMERCIAL FARMS IN THE VENDA REGION OF SOUTH AFRICA

The mango growers of the Venda region of South Africa are small-scale emerging commercial mango producers. In a mixed cropping system, maize, beans and other vegetables are grown between the mango trees. The production of these crops is mostly for household consumption, while mangoes are grown for selling purposes. This article analyses the economic situation of the farms, by conducting a financial analysis of the cash flow of the mango production, and comparing the farm indicators of two groups of farmers. The objective is to develop strategy proposals for strengthening the mango farm business. The financial analysis shows that the average selling price farmers receive for achar mangoes is not high enough to make investments in a mango orchard profitable. From the farm analysis it can be derived that farmers should increase specialisation in mangoes by planting more trees per hectare, and introduce pruning and a more adapted input use. Other possible strategies are the increase of management knowledge, improvement of the knowledge on farm production techniques, improvement of the farm environment and a better adaptation to the macroeconomic environment.

Problem‐solving strategies for small‐scale emerging commercial mango farmers in Venda1

This article describes the prevailing farming system of emerging small‐scale commercial mango farmers in the Venda region of the Northern Province of South Africa. Analyses based on intensive group discussions with farmers and prioritisation of their problems using a ‘problem tree/objective tree'framework, as well as comparisons with adjacent commercial mango farmers, enabled the development of operational support strategies. This study followed a new (for South Africa) participative approach. The first step of the Objective Oriented Intervention Planning (OOIP) approach to Logical Framework Analysis (LFA) is a problem tree; the next is to convert the problem tree into an objective tree in order to identify alternative strategies for alleviating constraints and problems. These are then summarised in the LFA matrix. This methodology is described here.