Transport Velocity Research Articles

The regulation of human organic anion transporter 4 by insulin-like growth factor 1 and protein kinase B signaling

Human organic anion transporter 4 (hOAT4), mainly expressed in the kidney and placenta, is essential for the disposition of numerous drugs, toxins, and endogenous substances. Insulin-like growth factor 1 (IGF-1) is a hormone generated in the liver and plays important roles in systemic growth, development, and metabolism. In the current study, we explored the regulatory effects of IGF-1 and downstream signaling on the transport activity, protein expression, and SUMOylation of hOAT4. We showed that IGF-1 significantly increased the transport activity, expression, and maximal transport velocity Vmax of hOAT4 in kidney-derived cells. This stimulatory effect of IGF-1 on hOAT4 activity was also confirmed in cells derived from the human placenta. The increased activity and expression were correlated well with the reduced degradation rate of hOAT4 at the cell surface. Furthermore, IGF-1 significantly increased hOAT4 SUMOylation, and protein kinase B (PKB)-specific inhibitors blocked the IGF-1-induced regulations on hOAT4. In conclusion, our study demonstrates that the hepatic hormone IGF-1 regulates hOAT4 expressed in the kidney and placenta through the PKB signaling pathway. Our results support the remote sensing and signaling theory, where OATs play a central role in the remote communications among distal tissues.

Preparation of a Bioinspired Conical Needle for Rapid Directional Water Transport and Efficient Fog Harvesting

Water shortages have become a serious issue that threatens the survival of humans, particularly in arid regions. Collecting fog from the air environment is an effective approach to obtaining freshwater resources. Herein, inspired by cactus spines, we fabricated a superhydrophilic conical copper needle by the combination of electrochemical etching and the SiO2 colloidal self-assembly method for water transportation and fog collection. It was found that a water film would be formed on the surface of the as-prepared copper needle once a water droplet was transported from the tip to the base, which acted as a lubricating film to reduce the frictional resistance between the following water droplets and the copper needle and promote the self-propelled movement of the droplets. As a result, the transport velocity and the longest transport distance of a single water droplet on the as-prepared copper needle were high up to 142 mm/s and 42 mm, respectively. Besides, the results reveal that the droplet transport distance decreased with the increase of apex angles, whereas it increased with the increase of droplet volume and tilt angles. Additionally, the as-prepared copper needle exhibited an outstanding water collection rate of ∼1700 mg/h for a single copper needle, which is five times that of the original copper needle. This work provides a facile and low-cost method to fabricate conical copper needles for water droplet manipulation and efficient fog collection, showing great application potential in the fields of microfluid, desalination, and freshwater collection.

High-speed directional transport of condensate droplets on superhydrophobic saw-tooth surfaces

HypothesisMost droplets on high-efficiency condensing surfaces have radii of less than 100 μm, but conventional droplet transport methods (such as wettability-gradient surfaces and structural-curvature-gradient surfaces) that rely on the unbalanced force of three-phase lines can only transport millimeter-sized droplets efficiently. Regulating high-speed directional transport of condensate droplets is still challenging. Therefore, we present a method for condensate droplet transportation, based on the reaction force of the superhydrophobic saw-tooth surfaces to the liquid bridge, the condensate droplets could be transported at high speed and over long distances. ExperimentsThe superhydrophobic saw-tooth surfaces are fabricated by femtosecond laser ablation and chemical etching. Condensation experiments and luminescent particle characterization experiments on different surfaces are conducted. Aided by the theoretical analysis, we illustrate the remarkable performance of condensate droplet transportation on saw-tooth surfaces. FindingsCompared with conventional methods, our method improves the transport velocity and relative transport distance by 1–2 orders of magnitude and achieves directional transport of the smallest condensate droplet of about 2 μm. Furthermore, the superhydrophobic saw-tooth surfaces enable multi-hop directional jumping of condensate droplets, leading to cross-scale increases in transport distances from microns to decimeters.

Chloroquine and Hydroxychloroquine, as Proteasome Inhibitors, Upregulate the Expression and Activity of Organic Anion Transporter 3.

Organic anion transporter 3 (OAT3), at the basolateral membrane of kidney proximal tubule cells, facilitates the elimination of numerous widely used drugs. Earlier investigation from our laboratory revealed that ubiquitin conjugation to OAT3 leads to OAT3 internalization from the cell surface, followed by degradation in the proteasome. In the current study, we examined the roles of chloroquine (CQ) and hydroxychloroquine (HCQ), two well-known anti-malarial drugs, in their action as proteasome inhibitors and their effects on OAT3 ubiquitination, expression, and function. We showed that in cells treated with CQ and HCQ, the ubiquitinated OAT3 was considerably enhanced, which correlated well with a decrease in 20S proteasome activity. Furthermore, in CQ- and HCQ-treated cells, OAT3 expression and OAT3-mediated transport of estrone sulfate, a prototypical substrate, were significantly increased. Such increases in OAT3 expression and transport activity were accompanied by an increase in the maximum transport velocity and a decrease in the degradation rate of the transporter. In conclusion, this study unveiled a novel role of CQ and HCQ in enhancing OAT3 expression and transport activity by preventing the degradation of ubiquitinated OAT3 in proteasomes.

Revisiting yield in terms of phloem transport to grains suggests phloem sap movement might be homeostatic.

Phloem sap transport, velocity and allocation have been proposed to play a role in physiological limitations of crop yield, along with photosynthetic activity or water use efficiency. Although there is clear evidence that carbon allocation to grains effectively drives yield in cereals like wheat (as reflected by the harvest index), the influence of phloem transport rate and velocity is less clear. Here, we took advantage of previously published data on yield, respiration, carbon isotope composition, nitrogen content and water consumption in winter wheat cultivars grown across several sites with or without irrigation, to express grain production in terms of phloem sucrose transport and compare with xylem water transport. Our results suggest that phloem sucrose transport rate follows the same relationship with phloem N transport regardless of irrigation conditions and cultivars, and seems to depend mostly on grain weight (i.e., mg per grain). Depending on the assumption made for phloem sap sucrose concentration, either phloem sap velocity or its proportionality coefficient to xylem velocity change little with environmental conditions. Taken as a whole, phloem transport from leaves to grains seems to be homeostatic within a narrow range of values and following relationships with other plant physiological parameters across cultivars and conditions. This suggests that phloem transport per se is not a limitation for yield in wheat but rather, is controlled to sustain grain filling.

Heat Transport and Convective Velocities in Compositionally Driven Convection in Neutron Star and White Dwarf Interiors

We investigate heat transport associated with compositionally driven convection driven by crystallization at the ocean–crust interface in accreting neutron stars, or growth of the solid core in cooling white dwarfs. We study the effect of thermal diffusion and rapid rotation on the convective heat transport, using both mixing length theory and numerical simulations of Boussinesq convection. We determine the heat flux, composition gradient, and Péclet number, Pe (the ratio of thermal diffusion time to convective turnover time) as a function of the composition flux. We find two regimes of convection with a rapid transition between them as the composition flux increases. At small Pe, the ratio between the heat flux and composition flux is independent of Pe, because the loss of heat from convecting fluid elements due to thermal diffusion is offset by the smaller composition gradient needed to overcome the reduced thermal buoyancy. At large Pe, the temperature gradient approaches the adiabatic gradient, saturating the heat flux. We discuss the implications for cooling of neutron stars and white dwarfs. Convection in neutron stars spans both regimes. We find rapid mixing of neutron star oceans, with a convective turnover time of the order of weeks to minutes depending on rotation. Except during the early stages of core crystallization, white dwarf convection is in the thermal-diffusion-dominated fingering regime. We find convective velocities much smaller than recent estimates for crystallization-driven dynamos. The small fraction of energy carried as kinetic energy calls into question the effectiveness of crystallization-driven dynamos as an explanation for observed magnetic fields in white dwarfs.

Temperature-dependent RNA editing in octopus extensively recodes the neural proteome

In poikilotherms, temperature changes challenge the integration of physiological function. Within the complex nervous systems of the behaviorally sophisticated coleoid cephalopods, these problems are substantial. RNA editing by adenosine deamination is a well-positioned mechanism for environmental acclimation. We report that the neural proteome of Octopus bimaculoides undergoes massive reconfigurations via RNA editing following a temperature challenge. Over 13,000 codons are affected, and many alter proteins that are vital for neural processes. For two highly temperature-sensitive examples, recoding tunes protein function. For synaptotagmin, a key component of Ca2+-dependent neurotransmitter release, crystal structures and supporting experiments show that editing alters Ca2+ binding. For kinesin-1, a motor protein driving axonal transport, editing regulates transport velocity down microtubules. Seasonal sampling of wild-caught specimens indicates that temperature-dependent editing occurs in the field as well. These data show that A-to-I editing tunes neurophysiological function in response to temperature in octopus and most likely other coleoids.

First-principles insights into the performance of high-entropy MXene as the anode for lithium ion batteries

High entropy MXene had four major effects, which had been widely studied in the field of cathode materials for lithium-ion batteries (LIBs). The enhancement of high entropy MXene's lithium storage performance was simply attributed to the cocktail effect or synergistic action of various metal elements, but the specific synergistic mechanism was still unclear. In this context, we used first-principal calculations to explore the effect of high entropy on the Li adsorption and diffusion on high-entropy MXene. We observed that (Ti0.25V0.25Nb0.25Mo0.25)2CO2 possessed higher electrical conductivity and ion transport velocity than other single-component MXene. Meanwhile, Li atom could be well adsorbed on the surface of MXene, and showed a low diffusion energy barrier (0.15 eV). Furthermore, the maximum possible capacity of high-entropy MXene was predicted to be 497 mAh/g, corresponding to 4 Li per unit cell. As a result, (Ti0.25V0.25Nb0.25Mo0.25)2CO2 should be a next generation anode material for LIBs.

Visualization of the initial radiocesium dynamics after penetration in living apple trees with bark removal using a positron-emitting 127Cs tracer

Following the Fukushima nuclear accident in March 2011, radiocesium (rCs) contamination in deciduous trees remains over 10 years later even though the trees were leafless at the time of the accident. This phenomenon is considered to be the result of repeated retranslocation of rCs that initially penetrated the bark into the internal tissues. To implement effective measures after a possible accident in the future, it is necessary to clarify how rCs is translocated in the tree after penetration. In this study, rCs translocation was dynamically visualized using a positron-emitting tracer imaging system (PETIS) and autoradiography after the bark of apple branches was removed. The PETIS results showed the translocation of 127Cs from the branch to young shoots and the main stem in apple trees under controlled spring growing conditions. The transport velocity of rCs in the branch was faster than that in the main stem. The transport of rCs, which was either acropetal or basipetal, in the main stem through the branch junction favored basipetal movement. Autoradiography of transverse sections of the main stem indicated that basipetal translocation was due to transport in the phloem. This study demonstrated the initial translocation responses of rCs similar to previous field research, which indicates that rCs transport to the young shoots tends to be higher under controlled conditions. Our laboratory-based experimental system may be useful to gain an improved understanding of rCs dynamics in deciduous trees.

An improved SPH scheme for the 3D FEI (Fluid-Elastomer Interaction) problem of aircraft tire spray

The problem of aircraft tire spray produced by taking off or landing on wet runways is widely concerned. When simulating this kind of FEI(Fluid-Elastomer Interaction) problem with traditional SPH((Smoothed Particle Hydrodynamics) method, there are some drawbacks in both fluid and elastomer calculation. For the elastomer simulation, the TL-SPH(Total Lagrangian-SPH) is used to deal with the deformation of tire under complicated motion and load. For the fluid simulation, a new SPH scheme of Riemann solver combined with transport velocity is proposed to solve the problem of particle clumping when the free surface is suffering from violent splash. Then the tire spray model by unified SPH is established, in which both the tire deformation and the spray pattern are validated by experiments. Finally, based on the large-scale tire spray simulation, the phenomenon of tire hydroplaning is studied.

Self-Complementary Hyperbolic Metasurface Antennas

Anisotropic Self-Complementary Metasurfaces (SCM) are structures constituted by a “self-dual” (according to Babinet’s duality principle) alternance of inductive and capacitive complementary strips. They support orthogonally polarized surface-wave modes with the same phase velocity in the principal direction of propagation. The isofrequency dispersion curves of these modes are hyperbolas, and therefore these metasurfaces (MTSs) fall in the category of hyperbolic MTSs. It is shown here that the hyperbolas may degenerate in same cases into almost straight lines, which implies that the velocity of energy transport is constantly directed along the complementary strips at any frequency and for any possible phasing orthogonal to the strips. In this circumstance, the SCM can be conveniently used to design dual-polarized leaky-wave antennas by modulating the impedances of the complementary strips. Each strip behaves as a channel, which can be independently fed with the desired phase and amplitude. We demonstrate here that these antennas exhibit a strong decoupling between both co-polar and cross-polar channels, even when the distance between strips is electrically small. This increases the performance of the antenna, especially in azimuthal beam scanning. A prototype at 2.6 GHz has been constructed and successfully measured confirming the theoretical conjectures.

A multifaceted approach to reveal the very-fine root's response of Fagus sylvatica seedlings to different drought intensities.

How temperate trees respond to drier summers strongly depends on the drought susceptibility and the starch reserve of the very-fine roots (<0.5 mm in diameter). We performed morphological, physiological, chemical, and proteomic analyses on very-fine roots of Fagus sylvatica seedlings grown under moderate- and severe drought conditions. Moreover, to reveal the role of the starch reserves, a girdling approach was adopted to interrupt the flux of photosynthates toward the downstream sinks. Results show a seasonal sigmoidal growth pattern without evident mortality under moderate drought. After the severe-drought period, intact plants showed lower starch concentration and higher growth than those subjected to moderate drought, highlighting that very-fine roots rely on their starch reserves to resume growth. This behavior caused them to die with the onset of autumn, which was not observed under moderate drought. These findings indicated that extreme dry soil conditions are needed for significant root death in beech seedlings and that mortality mechanisms are defined within individual compartments. The girdling treatment showed that the physiological responses of very-fine roots to severe drought stress are critically related to the altered load or the reduced transport velocity of the phloem and that the changes in starch allocation critically alter the distribution of biomass. Proteomic evidence revealed that the phloem flux-dependent response was characterized by the decrease of carbon enzymes and the establishment of mechanisms to avoid the reduction of the osmotic potential. The response independent from the aboveground mainly involved the alteration of primary metabolic processes and cell wall-related enzymes.

Spontaneous Transport Mechanics of Water Droplets under a Synergistic Action of Designed Pattern and Non-Wetting Gradient.

The controllable spontaneous transport of water droplets on solid surfaces has a broad application background in daily life. Herein, a patterned surface with two different non-wetting characteristics was developed to control the droplet transport behavior. Consequently, the patterned surface exhibited great water-repellant properties in the superhydrophobic region, and the water contact angle reached 160° ± 0.2°. Meanwhile, the water contact angle on the wedge-shaped hydrophilic region dropped to 22° after UV irradiation treatment. On this basis, the maximum transport distance of water droplets could be observed on the sample surface with a small wedge angle of 5° (10.62 mm), and the maximum average transport velocity of droplets was obtained on the sample surface with a large wedge angle of 10° (218.01 mm/s). In terms of spontaneous droplet transport on an inclined surface (4°), both the 8 μL droplet and 50 μL droplet could move upward against gravity, which showed that the sample surface possessed an obvious driving force for droplet transport. Surface non-wetting gradient and the wedge-shaped pattern provided unbalanced surface tension to produce the driving forces in the process of droplet transport, and the Laplace pressure as well is produced inside the water droplet during this process. This work provides a new strategy to develop a patterned superhydrophobic surface for droplet transport.

Magnetic Bucket Brigade Transport Networks for Cell Transport

AbstractControlled transport of biological cells in biomedical applications such as sorting, cell sequencing, and assembly of multicellular structures is a technological challenge. Research areas such as drug delivery or tissue engineering can benefit from precise cell location resulting in faster response rates or more complex tissue structures. Using computational methods, different soft magnetic elements with curved edges are designed to form a transport network, enabling transport and all functionalities for the manipulation of microbeads and cells on surfaces by rotational magnetic fields. Building blocks with bimodal functionalities due to segments of differently curved edges permit breakpoints as well as switchable transport via splitting and combining elements. Connecting the elements, networked paths are realized which allow variable movement patterns of magnetic carriers and cells. The direction of magnetic field rotation is altered to direct the beads and cells into different transport lines, and the exact timing is not critical. The networks are used to achieve deterministic movement of microbeads and cells with minimal intervention. Programmed transport over one millimeter with cell transport velocities of several micrometers per s is demonstrated. Based on scalable microchip technology, the networks can be integrated with CMOS‐compatible materials and straightforwardly combined with sensing and diagnostic structures.

A Numerical Approach to Characterize the Efficiency of Cyclone Separator

Cyclone separators are active filtering devices suitable for a variety of industrial applications from conventional cutting oil pumps to recycling liquids. Since the vortex flow inside cyclones is highly complicated, the performance and flow patterns of these filters should be thoroughly researched. Liquid–solid cyclones mostly use water. Numerical studies on cyclones using higher-viscosity oils are limited. In this study, a liquid–solid cyclone injected with medium-viscosity cutting oil containing various sized-particles was comprehensively investigated. The reliability of computational fluid dynamics (CFD) methods was verified through a comparison with the experimental results. Three models with different geometries were considered for the analysis. One model was used for CFD verification. The other two models involved adding sockets for hopper length extension and changing the shape of the bottom of the hopper. The models that changed the shape of the hopper, thus directly affecting the cyclone performance, were investigated, and each model was qualitatively compared using a validated method. In addition, particle separation efficiency was evaluated by focusing on the velocity distribution to quantitatively confirm the influence of changing the shape of the hopper. The tangential velocity was determined to be similar across all three models, while the axial velocity was different and the change in the velocity of transport of the particles affected the filter function.

Oocyte cumulus complex quality and oviduct transportation velocity in systemic autoimmune disease model mice.

Oocyte transportation by the oviduct involves the interaction between ciliated epithelial cells and cumulus cells. To determine whether the quality of cumulus-oocyte complexes (COCs) changes the transportation property of COCs, we compared the transportation velocity of COCs (TVC) by the infundibulum ex vivo with various combinations of infundibula and COCs collected from different mice. We used young and aged C57BL/6N and MRL/MpJ, and MRL/MpJ-Faslpr/lpr mice as the strains with intact female reproductive function and the systemic autoimmune disease model exhibiting oocyte pick-up dysfunction owing to the morphofunctional abnormality of ciliated epithelium, respectively. The TVC of aged MRL strains was less than that of aged C57BL/6N mice, suggesting that aging affects the transportation of COCs in MRL strains. The TVC of aged MRL/MpJ-Faslpr/lpr mice was the least among all examined combinations, whereas the TVC accelerated when the infundibulum or COCs were collected from other strains. These results indicate that the transportation property of COCs is determined not only by the ciliary function in the infundibulum but also by the properties of COCs.

Both network architecture and micro cracks effects on lacuno-canalicular liquid flow efficiency within the context of multiphysics approach for bone remodeling

When physical forces are applied to bone, its mechanical adaptive behaviors change according to the microarchitecture configuration. This leads to changes in biological and physical thresholds in the remodeling cell population, involving sensor cells (osteocytes) interacting with each other and changes in osteocyte shape due to variation in lacunar shape. The resulting alterations in fluid flow leads to changes in the membrane electrical potential and shear stress. Eventual creation of microcracks, may lead in turn to modify cell activity. In contrast, the redundancy in the lacuno canalicular network (LCN) interconnectivity maintains partial flow. Our goal was to investigate the role of fluid flow in LCN by proposing a model of electro-mechanical energy spread through inhomogeneous microarchitectures. We focused on mechano-sensitivity to changes in load-induced flow impacted by neighboring micro cracks and quantifying its critical role in changing, velocity, shear stress and orientation of liquid mass transportation from one cell to another. To enhance the concept of intricacy LCN micro-structure to fluid flow, we provide a new combined effects factor considered as osteocytes sensor efficiency. We customized an influence function for each osteocyte, coupling: in one hand, the spatial distribution within remodeling influence areas, conducting a significant fluid spread, leading hydro-dynamic behavior and impacted further by presence of micro cracks and; in other hand, the fluid electro kinetic behavior.As an attempt to fill the limitations stated by many of the recent studies, we reveal in numerical simulation, some results which cannot be measured in vitro/in vivo studies. Numerical calculations were performed in order to evaluate, among many others, how liquid flow conditions changes between lacunas, how the orientation and the magnitude of the governing flow in LCN can regulate osteocytes efficiency. In addition to be regulated by osteocytes, a direct effects of fluid flow are also acting on osteoblast activity. In summary, this new approach considers mechano-sensitivity in relation to liquid flow dynamic and suggests additional pathway for Osseo integration via osteoblast regulation. However, this novel modeling approach may help improve the mapping and design bone scaffolds and/or selection of scaffold implantation regions.

Cargo transport properties are enhanced by cylindrical microtubule geometry and elliptical contact zone on cargo surface

Molecular motors are responsible for carrying cellular transport of various membranous vesicles or organelles along cytoskeletal tracks. Transport of cellular cargos require high forces that are generated by motors working in groups. Hence, the properties of cargo transport can be modulated by varying various parameters such as cargo size and shape, microtubule geometry, motor number and their arrangement on cargo surface. Only those motors which are present in the contact zone on cargo surface have potential to bind to microtubule. Although earlier studies revealed the importance of cargo size, total motors attached to microtubule and their arrangement on cargo transport, yet how the contact zone influences binding of motors to microtubule largely remains unexplored. Here, it has been shown that contact zone is elliptical in shape for a spherical cargo and increases with cargo size for Kinesin-1 motors. To further understand the combined effect of elliptical contact zone and microtubule geometry on cargo transport, 3D mean-field model with uniform and clustered arrangement of motors for different cargo sizes and motor number has been used. Our findings indicate that cylindrical microtubule geometry maximizes the microtubule-bound motors which enhances the runlength and velocity of cargo transport. Our results show that microtubule-bound motors decrease with cargo size for uniform arrangement of motors on cargo thus decreasing its runlength and velocity, whereas in clustered arrangement, the number of microtubule-bound motors increase with cargo size which leads to increase in runlength and velocity.

Numerical analysis of sediment transport and depth averaged flow velocity in non-prismatic compound channels

The flow velocity in compound open channels is greatly affected by the geometry of both the main channel (MC) and floodplain (FP). In this study, a numerical investigation of the joint effect between the FP divergence angle and the size of suspended sediment particles on sediment transport and flow depth-averaged velocity (DAV) in non-prismatic diverging compound channels was conducted. In addition, the influence of relative flow depth on the DAV in different flow directions (i.e., stream-wise and span-wise) was examined. Three divergence angles of 4.0, 6.3 and 11.3°, four sediment particle sizes of 1, 5, 50 and 500 µm and two relative depths of the FP with respect to the MC of 0.20 and 0.40 were considered during simulations. A Computational Fluid Dynamics (CFD) software ANSYS-Fluent was used to perform the required simulation scenarios and the DAV was expressed in terms of a dimensionless velocity (i.e., relative depth-averaged velocity; RDAV) to generalize the use of the obtained results. Results revealed that the span-wise RDAV gradient between the MC and FP at the middle and the end sections of the divergence reach is much larger in the case of higher divergence angle. Moreover, the presence of coarse sediment particles in the sediment-laden flow resulted in an augmentation in the fluctuations of span-wise and stream-wise RDAVs as compared to those obtained in the case of fine sediment particles. Results also showed that the span-wise RDAV gradient between the two subsections (MC and FP) increases with decreasing the relative flow depth. Sediment particle size had a considerable impact on the span-wise RDAV values, especially in the case of lower relative depth. As the sediment particle size increases, the distortion in the span-wise RDAV distribution curves increases. However, the stream-wise RDAV along the MC and FP decreases, as the sediment particle size decreases, especially in the case of higher relative depth. Based on the results, it can be concluded that the span-wise DAV gradient was greatly affected by the FP divergence angle and sediment particle size. The fluctuations in the span-wise DAV gradient were more pronounced in the case of higher divergence angles and coarser sediment particles. The stream-wise DAV distribution along the MC and FP was mainly influenced by the relative depth and sediment particle size. The stream-wise DAV dip along the MC and FP was more explicit in the case of higher relative depths and finer sediment particles.

Impact of high- and low-flow nebulised saline on airway hydration and mucociliary transport.

Nebulised drugs, including osmotic agents and saline, are increasingly used during noninvasive respiratory support, including nasal high-flow therapy. The authors conducted an in vitro study to compare the hydration effect of nebulised isotonic 0.9% and hypertonic 7.0% saline on mucociliary transport. In a perfused organ bath, 10 sheep tracheas were exposed to 7.5 mL nebulised 0.9% and 7.0% saline entrained into heated (38°C) and humidified air delivered at high and low flow (20 and 7 L·min-1, respectively). Simultaneous measurements of the airway surface liquid height, mucus transport velocity, cilia beat frequency and surface temperature were made over time. The data are presented as mean±sd. The airway surface liquid height increased significantly with both 0.9% and 7.0% saline: at low-flow by 37.2±10.0 µm and 152.7±10.9 µm, respectively, and at high-flow by 62.3±5.6 µm and 163.4±25.4 µm, respectively (p<0.001). Mucus velocity was increased by both 0.9% and 7.0% saline from a baseline of 8.2±0.8 mm·min-1 to 8.8±0.7 mm·min-1 and 17.1±0.5 mm·min-1, respectively, with low-flow and at high-flow to 9.8±0.02 mm·min-1 (p=0.04) and 16.9±0.5 mm·min-1 (p<0.05), respectively. Ciliary beating did not change with 0.9% saline, but declined from 13.1±0.6 Hz to 10.2±0.6 Hz and 11.1±0.6 Hz (p<0.05) with 7.0% saline at low- and high-flow, respectively. The findings demonstrate that nebulised isotonic 0.9% saline, like hypertonic 7.0% saline, significantly stimulates basal mucociliary transport, and the use of high-flow delivery had no significantly different hydration effects compared with low-flow delivery. Hypertonic 7.0% saline suppressed ciliary beating, indicating an increase in airway surface liquid osmolarity, which may have negative effects on the airway surface with frequent use.