<h3>Study Objective</h3> To present the first robotic-assisted uterus retrieval from a living donor for uterine transplantation in Brazil. <h3>Design</h3> Stepwise demonstration of the technique with narrated video footage. <h3>Setting</h3> Three operating rooms were designated to carry out the transplantation: one for harvesting the donor's uterus by robotic surgery, another for the bench surgery, and another for the organ recipient. The donor was in the lithotomy position with steep Trendelenburg, intermittent pneumatic compression, and Allen Stirrups. She underwent robotic surgery with central docking. <h3>Patients or Participants</h3> The recipient was a 33-year-old patient with hypoplasia of the Müllerian duct system due to Mayer-Rokitansky-Küster-Hauser syndrome. The donor was a 50-year-old woman. <h3>Interventions</h3> Ureteral dissection was performed over its entire length. The vascular pedicles that emerged from the uterine arteries and the internal iliac arteries' posterior branches were clipped. The obliterated umbilical arteries, the internal iliac arteries, and the pelvic infundibulum ligaments were clipped and cut. The uterus was retrieved through a Pfannenstiel incision to avoid complications to the uterine vessels. The organ was transplanted in the recipient by end-to-end anastomosis of the internal iliac arteries and end-to-side anastomosis of the external iliac vein with a gonadal vein from the infundibulopelvic ligament. <h3>Measurements and Main Results</h3> Surgical intraoperative parameters were measured. The operative time from docking was 4 minutes. Robotic donor surgery took 400 minutes, bench surgery 62 minutes, and laparotomic recipient surgery was completed in 240 minutes. There were no intraoperative complications. The donor was discharged in 48 hours and the recipient in 5 days. <h3>Conclusion</h3> This is the first case in Brazil of uterine transplantation with a living donor to the best of our knowledge. Traditionally, patients who undergo uterine transplantation by minimally invasive surgery are managed by laparoscopy. However, this video demonstrates the feasibility of a robotic approach to uterine transplantation.