Vegetative Phase Research Articles

Phosphorylation of the transcription factor SlBIML1 by SlBIN2 kinases delays flowering in tomato.

Brassinosteroids (BRs) are well known for their important role in the regulation of plant growth and development. Plants with deficiency in BR signaling show delayed plant development and exhibit late flowering phenotypes. However, the precise mechanisms involved in this process require investigation. In this study, we cloned homologs of BRASSINOSTEROID INSENSITIVE 2 (SlBIN2), the GSK3-like protein kinase in tomato (Solanum lycopersicum). We characterized growth-related processes and phenotypic changes in the transgenic lines and found that SlBIN2s transgenic lines have delayed development and slow growing phenotypes. SlBIN2s work redundantly to negatively regulate BR signaling in tomato. Furthermore, the transcription factor SlBIN2.1-INTERACTING MYB-LIKE 1 (SlBIML1) was identified as a downstream substrate of SlBIN2s that SlBIN2s interact with and phosphorylate to synergistically regulate tomato developmental processes. Specifically, SlBIN2s modulated protein stability of SlBIML1 by phosphorylating multiple amino acid residues, including the sites Thr266 and Thr280. This study reveals a branch of the BR signaling pathway that regulates the vegetative growth phase and delays floral transition in tomato without the feedback affecting BR signaling. This information enriches our understanding of the downstream transduction pathway of BR signaling and provides potential targets for adjusting tomato flowering time.

Off-season production of Bell Pepper (Capsicum anuum, L.) as influenced by soil enhancers and mulching materials

In a study focused on off-season bell pepper (Capsicum annuum) production, 1,152 seedlings were sown, transplanted, and distributed across 48 plots measuring 4.4 sq.m. Each plot accommodated 24 seedlings, and meticulous care and observation were maintained throughout the study. Employing a 2x4 factorial design, this investigation aimed to assess the influence of soil enhancers and mulching materials on bell pepper cultivation. The data analysis was conducted using the analysis of variance in a randomized complete block design (RCBD), with mean comparisons facilitated through Duncan's Multiple Range Test (DMRT). The treatments consisted of two factors: Factor A involved soil enhancers, with Treatment A1 as the control, A2 as carabao manure, A3 as goat manure, and A4 as chicken manure. Factor B encompassed mulching materials, including B1 (control), B2 (rice hull), B3 (rice straw), and B4 (plastic mulching). The study's findings highlighted the substantial impact of soil enhancers, particularly chicken manure, on the vegetative growth phase of bell pepper. Furthermore, the combined application of soil enhancers and mulching materials, as well as individual applications, significantly affected the development of fruiting branches, with chicken manure particularly influencing the diameter of bell pepper fruits. In summation, the research suggests that the utilization of soil enhancers, especially chicken manure, constitutes a promising approach to enhance bell pepper vegetative growth during off-season production.

Deciphering the Role of SVP-Like Genes and Their Key Regulation Networks During Reproductive Cone Development in Pinus tabuliformis.

Reproductive development plays an essential role in the perpetuation of genetic material and environmental adaptation. In angiosperms, the Short Vegetative Phase (SVP) serves as a flowering repressor, influencing the development of floral organs. In this study, heterologous transformation of Arabidopsis thaliana with SVP-like genes (PtSVL1 and PtSVL2) derived from Pinus tabuliformis significantly impacted stamen formation and pollen fertility, without altering flowering time. Gene co-expression networks revealed that SVP-like and SOC1-like genes function as key coregulatory transcription factors during the initial stages of cone development in P. tabuliformis. Interestingly, the regulatory module of SOC1 regulated by SVP in angiosperms is absent in conifers and conifer SVP-like exercises its function in a form that is physically bound to SOC1-like. Furthermore, combining the yeast one-hybrid scanning with co-expression network analysis, revealed that SPLs and TPSs were the principal downstream target genes of PtSVL1. Notably, the PtSPL16 promoter is positively regulated by PtSVL1, and overexpression of PtSPL16 results in delayed flowering in Arabidopsis, suggesting that the PtSVL1-PtSPL16 module plays a crucial role in regulating reproductive development in conifers. Collectively, these findings enhance our understanding of the roles of SVP-like genes in conifers and the key regulatory networks centred on PtSVL1 during reproductive cone development.

Phenological growth stages of Amaranthus palmeri according to the extended BBCH scale

AbstractAmaranthus palmeri S. Watson is a problematic weed that has been encountered almost all over the world because of its stronger adaptability, which causes huge economic losses and local plant diversity decline. As an invasive plant, A. palmeri has been found throughout China. We thus make a code system to describe the phenological growth process of A. palmeri according to the extended BBCH scale to better understand the phenological growth of this weed in China. The growth process of A. palmeri was divided into vegetative growth phases and reproductive growth phases. The vegetative growth phases are composed of seed germination (0), leaf development (1), formation of side shoots (2) and main stem elongation (3). Likewise, the reproductive growth phases include inflorescence emergence (5), blooming (6), fruit development (7), seed maturity (8) and senescence (9). Based on the extended BBCH scale, the first digit stands for the primary growth stages, and the third digit represents the secondary growth stages. In particular, the second digit defines the growth progress: the number 0, means the vegetative growth phases, and the number 1 stands for the reproductive growth phases. Owing to the fact that its seed can germinate multiple times within a year, a general illustration of the phenological development stages of A. palmeri germinating in different months was provided here to describe the growth dynamic characteristic at different times. These data are of great significance to develop effective strategies for weed control and management of A. palmeri.

QTL mapping to identify loci and candidate genes associated with freezing tolerance trait in Camelina sativa

Lack of freezing tolerance is a major constraint for the production of agronomically important Brassica species, particularly in the Northern Great Plains (NGP) of the United States and Canada. However, within the Brassicaceae family, winter germplasm of camelina have shown excellent freezing tolerance and overwinter potential in the NGPs. Differences in freezing tolerance between a winter variety (Joelle) and a spring variety (C046) of camelina appear to be controlled by a small number of dominant or co-dominant genes. To unravel the genetic mechanisms for the differences in freezing tolerance, 254 Recombinant Inbred Lines (RILs) were developed using reciprocal crosses between these two camelina varieties. The RIL population was phenotyped at the F7 stage for freezing tolerance under controlled conditions and genotyped by whole-genome skim sequencing. A one-way ANOVA test revealed a significant (P < 0.001) difference exists among the RILs for freezing tolerance. A significant and strong correlation (r = 0.60, P < 0.001) was also observed between freezing tolerance and flowering time, indicating that regulation of flowering time might also influence freezing tolerance in camelina. A de novo linkage map was constructed using 4507 SNP markers covering a total of 1208.5 cM map distance with an average of 0.3 cM distances between the markers, which formed 20 linkage groups representing the 20 chromosomes (Chr) of C. sativa. The QTL analyses using three different programs revealed significant loci on Chr 8, 11, 13, 16 and 18 with LOD threshold value of over 3.5 for freezing tolerance. The QTL peaks with the greatest LOD values of 20.7 and 26.8 were observed at Chr 8 and Chr 13 and accounted for 18.3 % and 25.3 % of the phenotypic variation respectively. A total of 3369 annotated camelina genes were identified within ±50 Kb from the consensus QTL intervals generated from the output of the three mapping programs. Among them, 125 were transcription factors including twelve MIKC_MADS on Chr 8, 11, 13, 16 and 18 and two that annotate as the floral regulators FLOWERING LOCUS C (FLC) on Chr 8 and 13, an orthologue of MADS AFFECTING FLOWERING 3 and 4 (MAF4 and MAF3) of arabidopsis on Chr18, and an orthologue of SHORT VEGETATIVE PHASE (SVP) on Chr 16. Although many of the candidate genes identified near the freezing tolerance QTLs have previously been associated with flowering time, further studies are needed to help unravel how these genes impact freezing tolerance mechanisms and improve freezing tolerance in camelina and other Brassica crop species.

Damage associated with the african rice gall midge (AFRGM), Orseolia oryzivora Harris and Gagne (Diptera : Cecidomyiidae) in an integrated cropping system at Bama, Western Burkina Faso

Orseolia oryzivora is a major insect pest of rice in Burkina Faso. This study aimed at assessing the incidence of this pest on an integrated irrigated rice cropping system in Bama, western Burkina Faso. The work was carried out during two consecutive wet cropping seasons, 2020 and 2021. The experiment was conducted using a randomized split-plot design in a latice design. The main plots (MP) were divided into secondary plots, made up of a combination of two factors: two rice varieties (FKR64 and Orylux6) and four types of crop management before rice (Mucuna, cowpea forage, organic matter and a no-crop control); the third factor, mineral fertilization (F0, F1, F2, F3) was distributed in secondary plots (SP). Weekly entomological assessments were carried out from day 28 after transplanting (DAT) to DAT 84. Symptoms of O. oryzivora damage were assessed during the vegetative and reproductive phases of rice and examined in the laboratory. Results showed that all rice varieties were infested with O. oryzivora. The higher amount of NPK, the higher the damage caused by the insect pest. The FKR64 variety recorded the highest average rate of pre-imaginal O. oryzivora populations. The results of this study could contribute effectively to the sustainable management of O. oryzivora in Bama.

Phenology and performance of okra (Abelmoschus spp.) morphotypes from Benin

Okra is currently a highly profitable crop for the farming community because of its high consumption potential across rural and urban areas. Thus, it is important to evaluate the yield of various morphotypes of the three species grown in Benin. Assessing the production yield will enable efficient selection of the best morphotypes for the benefit of the population. A total of 53 morphotypes, characterized from okra species cultivated in Benin, were sown in a field at the University of Abomey-Calavi. For each morphotype, 5 plants were selected, and data were collected from germination until the fruits reached total senescence. Among the three species of okra cultivated in Benin, A. moschatus has the longest vegetative phase (68 ± 3 days). The average flowering time ranges from 51 days in A. caillei to 71 days in A. moschatus. The longest average fruiting time was also observed in A. moschatus (48 days). The analysis of variance showed that the duration of fruit set varied significantly among the species (p = 0.003). In terms of production yield, A. esculentus is the most productive species, yielding 10.50 tonnes of fruit per hectare. It is followed by A. moschatus and A. caillei, which yield 5.9 tonnes and 5 tonnes of fruit per hectare, respectively. Morphotype M25 of A. esculentus is the most profitable among the 53 morphotypes of the three species, producing 520.23 grams per stem, or 20.8 tonnes of fruit per hectare. Through this study, an annual sowing calendar was developed, enabling producers to plan okra cultivation for the entire year.

The Role of Mycorrhizal Fungi in Orchids Mycobiont and Orchids.

In nature, orchid plants are obligate myco-heterotrophs, and rely on mycorrhizal nutrient resources to grow and sustain in the wild, until they become physiologically active photosynthetic plants. Their seeds lack nutrient reserves and receive the necessary carbon from symbiotic fungi during germination. A mycorrhizal fungus provides nutrients, especially sugars, as well as water to the corresponding host plant. The range and distribution of orchid mycorrhizal fungi influence the survivability of orchid populations in their natural habitats. Mycorrhizae form symbiotic connections with the parenchymatous tissues of the roots of orchid plants. The objective of this study was to examine the presence of mycorrhiza in the roots of Aerides multiflora during the vegetative phase Methods: Fresh roots were hand-sectioned, and thin sections were observed under the microscope to locate the presence of mycorrhiza. Simultaneously, to observe the expansion of mycorrhiza in the cortical region. During the vegetative phase of plant growth, a peloton-like structure forms within the cortical region of the orchid roots. Mycorrhizae was observed to be distributed throughout the cortical layer of the root. This communication reviews the role of mycorrhiza in orchid plants.

The effector SJP3 interferes with pistil development by sustaining SHORT VEGETATIVE PHASE 3 expression in jujube.

Jujube witches' broom (JWB) is a phytoplasma disease that causes severe damage to jujube (Ziziphus jujuba) crops worldwide. Diseased jujube plants show enhanced vegetative growth after floral reversion, including leafy flower structures (phyllody) and the fourth whorl converting into a vegetative shoot. In previous research, secreted JWB protein 3 (SJP3) was identified as an inducer of phyllody. However, the molecular mechanisms of SJP3-mediated pistil reversion remain unknown. Here, the effector SJP3 was found to interact with the MADS-box protein SHORT VEGETATIVE PHASE 3 (ZjSVP3). ZjSVP3 was expressed in young leaves and during the initial flower bud differentiation of healthy jujube-bearing shoots but was constitutively expressed in JWB phytoplasma-infected flowers until the later stage of floral development. The SJP3 effector showed the same expression pattern in the diseased buds and promoted ZjSVP3 accumulation in SJP3 transgenic jujube calli. The N-terminal domains of ZjSVP3 contributed to its escape from protein degradation in the presence of SJP3. Heterologous expression of ZjSVP3 in Nicotiana benthamiana produced typical pistil abnormalities, including trichome-enriched style and stem-like structures within the leaf-like ovary, which were consistent with those in the mildly malformed lines overexpressing SJP3. Furthermore, ectopic expression of ZjSVP3 directly bound to the zinc finger protein 8 (ZjZFP8) and MADS-box gene SHATTERPROOF 1 (ZjSHP1) promoters to regulate their expression, resulting in abnormal pistil development. Overall, effector SJP3-mediated derepression of ZjSVP3 sustained its expression to interfere with pistil development, providing insight into the mechanisms of pistil reversion caused by JWB phytoplasma in specific perennial woody plant species.

Nitrogen modulates the ozone response of Mediterranean wheat: Considerations for ozone risk assessment

The experiment was conducted in an Open Top Chamber facility located in the Mediterranean basin to investigate how nitrogen (N) fertilization affects the response of wheat to ozone (O3) exposure. The study considered the response of Artur Nick, a modern wheat cultivar commonly used in the area, to three O3 exposure levels (ambient and elevated ambient, +20 and +40 nL L−1 O3), and two N fertilization doses (100 and 200 kg ha−1). Measurements included leaf gas exchange, leaf chlorophyll content, leaf and grain N content, plant growth and yield parameters. Ozone × N interactive effects were studied and quantified based on accumulated O3 concentrations above a 40 nL L−1 threshold (AOT40) and phytotoxic O3 dose (POD) indices, which are used in O3-risk assessments, from which critical levels (CL) for a 5 % effect were derived.Results revealed that O3 impacts on growth and yield parameters were stronger under the highest N fertilization dose. In consequence, O3 Critical Levels (CL) were as much as 3–4 times lower for grain yield in the high-N compared to the low-N treatment. Interestingly, O3 limited the fertilizer stimulus, strongly reducing the N use efficiency for grain yield and the agronomic efficiency of N for protein yield. Another important aspect was that 71 % of the POD was accumulated before anthesis, stressing the potential importance of O3 exposure during the vegetative phase of wheat under Mediterranean conditions, which is usually considered less important than post-anthesis exposure.In conclusion, this study suggests the need to consider crop N management in the derivation of O3 CLs, due to its effect on dose-response relationships used for CL derivation, including the potential O3 effects in N use efficiency. Therefore, N modulation could be considered in the O3-risk assessment methodology to be applied in risk exercises when negotiating air pollution abatement policies.

Utilizing Principal Component Analysis to Assess the Effects of Complex Foliar Fertilizers Regarding Maize (Zea mays L.) Productivity

This study sought to determine the effects of foliar fertilization (FF) on both the quantity and quality of maize grains using principal component analysis (PCA). This chemometric approach enabled the selection of the best foliar treatment model for enhancing maize yield and quality. The results were analyzed via PCA, providing valuable insights into identifying the FF recipe with the greatest influence on maize grain production and quality. These field experiments were run during the time period 2020–2022 in the university’s experimental field. Seven experimental variants with three repetitions were tested, including a control group and various FF formulations labeled V1 through V7, each with different chemical compositions. FF applications were conducted during specific vegetative phases of the maize, respectively, in stages 15–16 BBCH (5–6 unfolded leaves) and 20–22 BBCH (10–12 unfolded leaves), with application rates varying from 2 to 6 Lha1 according to the product’s chemical properties. The application of FF treatments positively impacted both the production and quality of maize grains, as evidenced by specific quality indices such as moisture, protein, lipid, carbohydrate, fiber, and mineral content.

Composition of Parasitoid Wasps in Insect Pests of Rice (Rice Leaffolder, Cnaphalocrocis Medinalis Guenée)

Biological control is one aspect of Integrated Pest Management (IPM). The method focusses on natural enemies; beneficial insects are applied for biological control of agricultural insect pests and also provide an environmentally friendly approach. For the rice pests including Rice Leaf Folder (RLF), several parasitoids tend to be natural enemies that could be used to control the pest from the egg stage to pupa. This study was conducted with two varieties of rice, namely “Sen Kra Oub” and “Sro Ngea” at Battambang Province of Cambodia. Parasitoid wasps were collected from egg to pupa stages of RLF and rice yellow stem borer by rearing method, and stored in 70% of alcohol solution before sending for identification in Vietnam’s Institute of Ecology and Biological Resources (IEBR) Laboratory. The outcomes revealed that five hymenopteran parasitic wasps of three different families were found, viz. Apanteles cypris Nixon, Bracon onukii Watanabe and Pentatermus striatus (Szepligeti) (Braconidae), Xanthopimla flavolineata Cameron (Ichneumonidae), and Telenomus rowani Gahan (Scelionidae) and being the primary parasitoids of rice leaffolder, Cnaphalocrocis medinalis Guenee; stemborer Scirpophaga incertulas Walker (Pyralidae) and black branded swift Pelopidas mathias (Hesperiidae). Parasitism was not found at the vegetative phase with Sen Kra Ob varieties but in reproductive phase. parasitism proportion was 16.66% and 28.57% to 50% in ripening phase, different from Sro Ngea variety where a proportion was found in vegetative phase 27.27%, reproductive phase at 28.57% to 40% and 28.57% to 43% in ripening phase. All the hymenopteran parasitoids were initially recorded in Cambodia, whereby all the parasitoids were potential agents for biological control for important rice insect pests. The outcome of this research suggests that the Government should invest more in the research and development for biological control in Cambodia.

Allele-Specific Hormone Dynamics in Highly Transgressive F2 Biomass Segregants in Sugarcane (Saccharum spp.).

Sugarcane holds global promise as a biofuel feedstock, necessitating a deep understanding of factors that influence biomass yield. This study unravels the intricate dynamics of plant hormones that govern growth and development in sugarcane. Transcriptome analysis of F2 introgression hybrids, derived from the cross of Saccharum officinarum "LA Purple" and wild Saccharum robustum "MOL5829", was conducted, utilizing the recently sequenced allele-specific genome of "LA Purple" as a reference. A total of 8059 differentially expressed genes were categorized into gene models (21.5%), alleles (68%), paralogs (10%), and tandemly duplicated genes (0.14%). KEGG analysis highlighted enrichment in auxin (IAA), jasmonic acid (JA), and abscisic acid (ABA) pathways, revealing regulatory roles of hormone repressor gene families (Aux/IAA, PP2C, and JAZ). Signaling pathways indicated that downregulation of AUX/IAA and PP2C and upregulation of JAZ repressor genes in high biomass segregants act as key players in influencing downstream growth regulatory genes. Endogenous hormone levels revealed higher concentrations of IAA and ABA in high biomass, which contrasted with lower levels of JA. Weighted co-expression network analysis demonstrated strong connectivity between hormone-related key genes and cell wall structural genes in high biomass genotypes. Expression analysis confirmed the upregulation of genes involved in the synthesis of structural carbohydrates and the downregulation of inflorescence and senescence-related genes in high biomass, which suggested an extended vegetative growth phase. The study underscores the importance of cumulative gene expression, including gene models, dominant alleles, paralogs, and tandemly duplicated genes and activators and repressors of disparate hormone (IAA, JA, and ABA) signaling pathways are the points of hormone crosstalk in contrasting biomass F2 segregants and could be applied for engineering high biomass acquiring varieties.

Water Productivity of Mustard Green (Brassica juncea L.) with Variation of Irrigation Systems

Drip irrigation and self-watering are two examples of irrigation technology improvements that employ effective and efficient watering methods. Water productivity may be used as a benchmark to compare irrigation efficiency and agricultural productivity. The purpose of this study was to assess mustard green's water productivity under conventional, drip, and self-watering irrigation systems. The effect of irrigation variation on mustard green growth was studied using a nonfactorial technique with a completely randomized design (CRD). The design has three treatments and six replications. This study examined the following variables: height, number of leaves, yield, irrigation water utilized, and water productivity of mustard green. The study found that mustard green plants require 0.69 mm/day of water in the vegetative phase, 2.83 mm/day in the generative phase, and 1.69 mm/day in the final phase. The use of different watering systems has a significant influence on mustard green's height and leaf number. Self-watering at 15 g/L provides the maximum water productivity for mustard green, followed by drip irrigation at 8.46 g/L and conventional irrigation at 7.69 g/L. Keywords: Drip irrigation, Mustard green, Self-watering irrigation, Water productivity

Stable early heading in photoperiod-insensitive rice varieties results from an extremely short photoperiod-sensitive phase and weak temperature sensitivity

The transition from vegetative to reproductive growth in rice (Oryza sativa) is mainly regulated by photoperiod and temperature; however, it remains unclear how photoperiod-insensitive (photo-In) early heading rice varieties make this transition. Vegetative growth consists of a basic vegetative phase (BVP) and a photoperiod-sensitive phase (PSP). A certain duration of the BVP is required prior to the PSP, which varies according to daylength conditions. PSP duration is conventionally used as a parameter of photoperiod sensitivity, but this is not applicable to photo-In rice varieties. Here, we aimed to characterize the PSP in photo-In rice varieties. We examined four photo-In varieties and photoperiod-sensitive (photo-Se) varieties grown in four controlled environments, evaluating the lengths of their growth phases based on the turning point of the interval of leaf emergence and panicle initiation (PI). The photo-In varieties had an extremely short PSP, regardless of daylength, compared with the photo-Se varieties. Low temperature uniformly prolonged all growth stages in the photo-In varieties but led to a much longer PSP in the photo-Se varieties. The photo-In varieties, each carrying a non-functional allele of Ghd7, lost the ability to suppress PI, resulting in early heading. Hemi-knockout plants at the Ghd7 locus demonstrated the earlier heading than the wild-type plant due to shortening of PSP. Our results suggest that the photo-In varieties begin PI immediately after the end of the BVP, but their flowering is not a temperature-dependent process.

Влияние биологических регуляторов роста на содержание фотосинтетических пигментов Campanula alliariifolia

Abstract. The purpose is to study the effectiveness of the action of the natural signaling molecule salicylic acid (SA) and Bacillus subtilis (Bs) on the ratio of photosynthetic pigments (PhSP) in plant materials (leaves) of Campanula alliariifolia Willd. and its decorativeness. Methods. Using spectrophotometric techniques, an analysis was carried out of the dynamics of the content of pigments (chlorophyll a, chlorophyll b and carotenoids) under the influence of a strain of the bacterium Bacillus subtilis, isolated at the Bashkir Scientific Research Institute of Agriculture of the Federal Research Center of the Russian Academy of Sciences (Ufa) from the arable soil layer of the Republic of Bashkortostan, and its combination with salicylic acid during the growing season. Scientific novelty. The influence of the B. subtilis strain separately and together with salicylic acid on the physiological parameters and decorative properties of Campanula alliariifolia in the conditions of the Bashkir Cis-Ural was studied. Results. The content and ratio of PhSP in dynamics (phases of budding, flowering and fruiting) were analyzed. It was revealed that inoculation of C. alliariifolia plants with Bacillus subtilis significantly increases the amount of carotenoids during the flowering phase. During the fruiting phase, the maximum content of PhSP was noted in the Bs + SA variant of the experiment. Two-factor analysis of variance showed that the significant influence of factor A (vegetation phase), factor B (different experimental options), and the degree of total influence of two factors (A × B) on the content of PhSP in samples of campanula leaves is statistically significant for the studied indicators. It was found that inoculation with the B. subtilis strain maximized indicators such as the abundance of flowering and the density of bellflower inflorescences, thereby improving the decorativeness of C. alliariifolia in the conditions of the Bashkir Cis-Ural.

ISOLASI DAN UJI AKTIVITAS BAKTERI PELARUT FOSFAT DARI RHIZOSFER TANAMAN PADI (Oryza sativa L.) PADA FASE VEGETATIF DAN GENERATIF

Phosphorus (P) is a very important macronutrient for plant growth and development. Plants cannot fully absorb phosphate in the soil, only 25% can be absorbed by plants while Al binds 75% in the soil. Plants absorb P from the soil in the form of phosphate ions, especially in the form of H2PO4- and HPO42- which are present in soil solutions. H2PO4 ion is more common in acidic soils, while higher pH (&gt; 7) forms HPO42- is more dominant. The lack of P dissolved in the soil causes the plant to get P intake from other sources such as fertilizers. Efforts to increase the efficiency of Phosphate dissolution, currently the use of Phosphate solubilizing microbes have begun to be developed. This study aims to determine the number of isolated bacterial colonies, the ability of phosphate-solubilizing bacteria, and the levels of P-available before and after giving phosphate-solubilizing bacterial isolates. This research was conducted at the Laboratory of Plant Pests and Diseases and in the Laboratory of Soil and Environmental Conservation, Faculty of Agriculture, Universitas Muslim Indonesian, Makassar, Indonesia, from November to December 2020. The materials used in this study were HVS paper, aluminum foil, plastic wrap, labels, rubber, Pikovskaya media, sterile distilled water, plastic, cotton, tissue, 70% alcohol, rubbing alcohol, NA (Natrium Agar) media, soil samples from the vegetative and generative phases of rice rhizosphere from Tebba Village, Salomekko District, Bone Regency. This research method is a qualitative exploratory research based on data analysis. The results of this study indicated that the vegetative phase 3 rice isolates with 10-5 dilution had the highest number of colonies, namely 9,200,000 CFU /ml. Plant generative phase 3 rice isolate at 10-5 dilution had the highest average phosphate solubility with an IPF value of 5.56. Plant generative phase 3 rice isolate had the highest average P-available before and after the addition of Phosphate Solubilizing Bacteria isolates, namely 3.83 ppm, and after the addition of Phosphate Solubilizing Bacteria isolates increased to 9.58 ppm.

Emisi Metana (CH4) pada Beberapa Metode Pengelolaan Limbah Sawah di Kecamatan Anjir Pasar Kabupaten Barito Kuala

Paddy fields for all of Indonesia are 8.1 million ha, about 43% are in Java and about 57% are outside Java. Paddy fields are part of the wetlands. Paddy fields control the global climate through the gases they produce and have a greenhouse effect. One of the greenhouse gases is methane, therefore the purpose of this study was to determine the effect of rice field waste management on methane emissions and the population of methanogenic microorganisms. The research method used is one-factor Completely Randomized Design (RAL). The factor tested was the method of managing rice waste before planting rice with five treatments namely: A = Rice field waste was slashed and then lifted into the mound after a few days of being returned to the field; B = Rice field waste is carried out management slashed, rolled up, reversed, and stretched; C = Rice field waste is slashed, planted with traces and then slashed again after the trace is transferred to land outside the research plot; D = Rice field waste sprayed with herbicide 2 times; E = Ricefield waste sprayed with herbicide 1 time and then soil in the tractor. Each treatment was repeated four times so 20 units of the experimental. CH4 gas retrieval used the hood method while the microorganism population used the MPN (most probable Number) method. The results showed that the rice field waste management method had an effect on methane emissions and populations of methanogen microorganisms in the planting and vegetative phases, while the generative phase had no effect.

Pengaruh Dosis Phospor Dan Pupuk Cair Pada Tanaman Bunga Kol (Brassica Oleracea Var. Botrytis L)

Cauliflower is a vegetable that has commercial value and excellent prospects for public consumption. According to the Central Statistics Agency of the Republic of Indonesia, cauliflower production has decreased from 2019 to 2021, therefore it is necessary to improve maintenance, one of which is by providing Phosphorus fertilizer to increase productivity. This will increase the rate of photosynthesis to produce high productivity and the correct dose of liquid organic fertilizer (POC) to maximize growth in the vegetative and generative phases thereby increasing the yield of cauliflower plants. This research aims to determine the effect of giving various doses of Phosphorus and POC fertilizers on the growth and yield of cauliflower plants. The research was carried out in the village, Girilayu, Matesih, Karanganyar from February to May 2024. Using the Complete Randomized Block Design (RAKL) method consisting of 2 treatment factors and 3 replications. The first factor is the dosage of Phosphorus (P) fertilizer P1: 100 kg/ha, P2: 150 kg/ha, P3: 200 kg/ha. The second factor is the dose of POC (C), C1: 4 cc/L, C2: 8 cc/L, C3: 12 cc/L. The results research showed (1) Giving various doses of Phospor fertilizer had a very real effect on fresh stover, dry stover, plant diameter, plant weight per plant, and crop weight per plot. However, it does not have a real effect on plant height and number of leaves. (2) Giving various doses of POC had a very real influence on fresh stover, dry stover, plant diameter, and plant weight per plant had a real influence on crop weight per plot and had no real influence on plant height and number of leaves. (3) The interaction between treatments had a very real influence on fresh stover, dry stover, and plant weight per plant, and had no real influence on plant height, number of leaves, plant diameter, and weight per plant plot. (4) The highest results were obtained in the branch pruning treatment 3 weeks after planting with an NPK fertilizer dose of 200 kg/ha, namely a plant weight of 11,997.4 grams per plot (26,644 kg/ha).

PRELIMINARY RESULTS REGARDING THE EFFECTS OF GREEN MANURES ON WHEAT CULTIVATION ON THE ACIDIC SOILS OF THE NORTH-WEST REGION OF ROMANIA

This study addressed the burgeoning interest in ecological agriculture, emphasizing sustainable and regenerative practices. Among these, the utilization of green manures emerged as pivotal for enhancing soil health and augmenting the yield of nutritionally dense crops. Five plant species (triticale, peas, soybeans, sunflower, and rapeseed) were selected for incorporation, sown during the summer, and subsequently integrated into the soil while still in their vegetative phase, preceding the optimal sowing window for wheat. The experiment encompassed three repetitions for the principal crop, of wheat implemented through split-plot design. Each repetition comprised both a fertilized and an unfertilized plot, further sub-divided, with certain segments receiving fungicidal treatment and others left untreated. The experimental setup was situated on an albic luvisol, exhibiting humus content ranging from 1% to 1.8% in the plow layer, coupled with an acidic pH range of 4.2 to 5.3. The trial site was located within the experimental fields of the Plant Protection Laboratory at the Livada Agricultural Research and Development Station, Satu Mare County. The primary aim of this experiment was to evaluate the influence of green manures, including their synergistic interplay with chemical fertilizers (calcium ammonium nitrate 27% N), on wheat. Evaluative parameters encompassed yield metrics, weed infestation levels, disease and pest resistance, along with the quality attributes of the principal crops. Ultimately, the study sought to furnish a encompassing comprehension of the advantages conferred by this regenerative agricultural practice, within the contemporary framework of ecological and agronomic imperatives. In conclusion, the inaugural year of experimenting with green manures did not yield an immediate augmentation in crop yield. However, it became evident that additional fertilization was required to fully harness the benefits of green manures. Encouragingly, the data spotlighted a propitious finding—green manures bolstered the disease resistance of wheat, implying enduring advantages for crop health. Furthermore, the adoption of rapeseed and peas as green manure crops, demonstrated a noteworthy reduction in weeding, affording an economical and pragmatic edge for sustainable agricultural practices. Additionally, when amalgamated with chemical fertilizers, the interaction with green manures evinced an augmented production capacity, underscoring the potential for a synergistic strategy in maximizing crop output. These revelations highlight the significance of a holistic and integrated approach to agricultural methodologies, underscoring the value of amalgamating green manures with judicious fertilization strategies. As we persist in our exploration and refinement of these techniques, the potential for enhancing both productivity and sustainability in our agricultural systems remains considerable, ultimately contributing to a more resilient and efficient food production process.