Vegetative Organs Research Articles

Morphological and Anatomical Characteristics of the Species Paliurus spina-christi

In plants and all living beings, the fundamental influence comes from ecological factors that operate simultaneously and in a complex manner. The influence of these factors is expressed in the morphological and anatomical features of the vegetative organs of plants, especially the leaves, which are among the most delicate organs where a series of adaptations occur. Although biological science today recognizes approximately 500,000 plant species, they differ from each other morphologically, anatomically, and genetically because they live in different life conditions and circumstances. Understanding the morphological‒anatomical characteristics of plants is highly important for determining their taxonomic and ecological adaptability. This connection between the morphological-anatomical structure of vegetative organs, especially leaves, and external factors has been addressed by many authors: Lakusiç (1962), Greb (1957), Pavlov (1965), Hoxha (1995), Mustafa (1995), and others. To obtain a clearer picture of the impact of ecological factors on the morphological and anatomical characteristics of vegetative organs of plants, we conducted research on the anatomical structure of leaves from different insertions: upper (E) and middle (M) of the plant species Pailurus spina Christi.

Does Nitrogen Fertilization Improve Nitrogen-Use Efficiency in Spring Wheat?

To investigate the effects and mechanism of prolonged inorganic nitrogen (N) fertilization on the N-use efficiency of spring wheat (Triticum aestivum L.), a long-term study initiated in 2003 was conducted. The study analyzed how N fertilization affects dry matter translocation, N translocation, soil NO3-N, and N-use efficiency. Five different N-fertilizer rate treatments were tested: N0, N52.5, N105, N157.5, and N210, corresponding to annual N fertilizer doses of 0, 52.5, 105.0, 157.5, and 210.0 kg N ha−1, respectively. Results showed that increasing N-fertilizer rates significantly enhanced the two-year average dry matter accumulation amount (DMA) at maturity by 22.97–56.25% and pre-flowering crop growth rate (CGR) by 17.11–92.85%, with no significant increase beyond 105 kg N ha−1. However, no significant correlation was observed between the dry matter translocation efficiency (DTE) and wheat grain yield. Both insufficient and excessive N applications resulted in an imbalanced N distribution favoring vegetative growth over reproductive growth, thus negatively impacting N-use efficiency. At maturity, the N-fertilized treatments significantly increased the two-year average N accumulation amount (NAA) by 52.04–129.98%, with no further increase beyond 105 kg N ha−1. N fertilization also improved the two-year average N translocation efficiency (NTE) by 56.89–63.80% and the N contribution proportion (NCP) of wheat vegetative organs by 27.79–57.83%, peaking in the lower-N treatment (N52.5). However, high-N treatment (N210) led to an increase in NO3-N accumulation in the 0–100 cm soil layer, with an increase of 26.27% in 2018 and 122.44% in 2019. This higher soil NO3-N accumulation in the 0–100 cm layer decreased NHI, NUE, NAE, NPFP, and NMB. Additionally, N fertilization significantly reduced the two-year average N harvest index (NHI) by 9.89–12.85% and N utilization efficiency (NUE) by 11.14–20.79%, both decreasing with higher N application rates. The NAA followed the trend of anthesis > maturity > jointing. At the 105 kg N ha−1 rate, the highest N agronomic efficiency (NAE) (9.31 kg kg−1), N recovery efficiency (NRE) (38.32%), and N marginal benefit (NMB) (10.67 kg kg−1) were observed. Higher dry matter translocation amount (DTA) and N translocation amount (NTA) reduced NHI and NUE, whereas higher NTE improved NHI, NUE, and N partial factor productivity (NPFP). Overall, N fertilization enhanced N-use efficiency in spring wheat by improving N translocation rather than dry matter translocation.

Ergothioneine Improves Seed Yield and Flower Number through FLOWERING LOCUS T Gene Expression in Arabidopsis thaliana.

Biostimulants are a new category of materials that improve crop productivity by maximizing their natural abilities. Out of these biostimulants, those that increase seed production are considered to be particularly important as they contribute directly to the increase in the yield of cereals and legumes. Ergothioneine (EGT) is a natural, non-protein amino acid with antioxidant effects that is used in pharmaceuticals, cosmetics, and foods. However, EGT has not been used in agriculture. This study investigated the effect of EGT on seed productivity in Arabidopsis thaliana. Compared with an untreated control, the application of EGT increased the seed yield by 66%. However, EGT had no effect on seed yield when applied during or after bolting and did not promote the growth of vegetative organs. On the other hand, both the number of flowers and the transcript levels of FLOWERING LOCUS T (FT), a key gene involved in flowering, were increased significantly by the application of EGT. The results suggest that EGT improves seed productivity by increasing flower number through the physiological effects of the FT protein. Furthermore, the beneficial effect of EGT on flower number is expected to make it a potentially useful biostimulant not only in crops where seeds are harvested, but also in horticultural crops such as ornamental flowering plants, fruits, vegetables.

The humic acids influence on the quantity and quality of table grape varieties under the Absheron peninsula conditions

Humates are widely used in viticulture, including ecological viticulture. Humic stuffs benefit the qualitative and quantitative indicators of the grape harvest and can reduce the use of synthetic fertilizers and pesticides. This article presents the results of tests on the foliar application of various doses of the “Rostok” fertilizer in the conditions of the Absheron zone of Azerbaijan on local varieties of table grapes – traditional Tabrizi and a selection variety of Scientific Research Institute of Viticulture and Wine-making Ganjavi, grown in the Ampelographic collection of the institute, located on the Absheron peninsula. Fertilizer was applied in three doses – 0.5; 1.0 and 1.5 liter per hectar. Under the influence of the fertilizer, such elements of yield as the vigor of shoot growth, the number of opened buds, fruitful shoots, the number of berries in a bunch, the weight of a bunch, as well as the sugar content of berry juice increased. Fertilizer application had a positive effect on eliminating signs of leaf chlorosis. No toxic effect on the vegetative organs of the grape plant, on its development, or on the beneficial insect fauna of the vineyard was noted. Based on the data obtained, as well as calculations of economic efficiency, a four-fold application of fertilizer (twice before flowering, once after, once during veraison) at the rate of 1 liter per hectare was recommended for widespread use in wine-growing farms.

Histochemical and ultrastructural localization of triterpene saponins in Medicago truncatula.

In the Medicago genus, saponins are complex mixtures of triterpene pentacyclic glycosides extensively studied for their different and economically relevant biological and pharmaceutical properties. This research is aimed at determining for the first time the tissue and cellular localization of triterpene saponins in vegetative organs of Medicago truncatula, a model plant species for legumes, by histochemistry and transmission electron microscopy. The results showed that saponins are present mainly in the palisade mesophyll layer of leaves, whereas in stems they are mostly located in the primary phloem and the subepidermal cells of cortical parenchyma. In root tissue, saponins occur in the secondary phloem region. Transmission electron microscopy revealed prominent saponin accumulation within the leaf and stem chloroplasts, while in the roots the saponins are found in the vesicular structures. Our results demonstrate the feasibility of using histochemistry and transmission electron microscopy to localize M. truncatula saponins at tissue and cellular levels and provide important information for further studies on biosynthesis and regulation of valuable bioactive saponins on agronomic relevant Medicago spp., such as alfalfa (Medicago sativa L.). RESEARCH HIGHLIGHTS: The Medicago genus represents a valuable rich source of saponins, one of the most interesting groups of secondary plant metabolites, which possess relevant biological and pharmacological properties. Plant tissue and cellular localization of saponins is of great importance to better understand their biological functions, biosynthetic pathway, and regulatory mechanisms. We elucidate the localization of saponins in Medicago truncatula with histochemical and transmission electron microscopy studies.

Cisuralian plant fossils from Khenifra (Central Morocco): Palaeoenvironmental and palaeobiogeographical reconstructions

This study focuses on newly collected plant fossils from the Cisuralian successions of the Khenifra Basin in Central Morocco, revealing a diverse assemblage dominated by conifers, pteridosperms, and sphenophytes. The assemblage includes eleven taxa of vegetative and reproductive organs preserved as compressions and impressions. Two pteridosperm species taxa (Neurodontopteris auriculata, Odontopteris subcrenulata) are recorded for the first time from Central Morocco, whereas other taxa are known from the early Permian deposits of Bou Achouch and Tiddas basins. Contrary to previous interpretations, the flora is does not exhibit a mixed character, but instead exhibits a distinct Euramerican affinity, despite the absence of the widespread callipterids Autunia conferta and A. naumannii. These similarities between this flora and coeval Euramerican floras highlight the significant biogeographical connections across Pangea during the Cisuralian. The plant fossil assemblage is dominated by meso- to xerophilous species adapted to drought, with rare hygrophilous elements adapted to humid habitats, reflecting a heterogeneous landscape with various vegetation types.

Combined application of organic and inorganic fertilizers sustained rice yields and N accumulation and decreased soil-canopy system NH3 emission

Excessive inorganic fertilization created various environmental problems, and the combination of organic and inorganic fertilization not only maintains crop production but is also environmentally sustainable. A pot experiment was conducted in Yangzhou, China, to investigate the comprehensive effects of organic fertilization combined with inorganic fertilization on the yield and yield components, nitrogen (N) uptake of different organs, and soil-plant system ammonia (NH3) emissions of two rice cultivars, taking into account the interception of soil NH3 emissions by the rice canopy. The treatments included the conventional practice of inorganic N applied by local farmers (INO, 270 kg N ha−1), 30 % reduced INO (70 %-INO, 189 kg N ha−1), and organic fertilizer combined with 70 %-INO (ORG-INO). Compared to 70 %-INO, ORG-INO significantly increased grain yield by 38 %, mainly due to more panicles. Similarly, ORG-INO also significantly promoted N uptake in the two rice cultivars, especially in terms of grain N accumulation, relative to 70 %-INO. The increase in grain N accumulation caused by ORG-INO was mainly due to enhanced post-anthesis N uptake, rather than N remobilization of vegetative organs, which also suggested that organic fertilizer could modify the processes of N retention and release. There was no significant difference in rice yield and N accumulation between INO and ORG-INO treatments, which also indicated that ORG-INO was effective in maintaining grain yield while reducing inorganic fertilizer input. Moreover, ORG-INO significantly reduced soil-plant system NH3 emissions, while the rice canopy intercepted approximately 30 % of soil NH3 emissions throughout the entire growing season. In summary, ORG-INO treatment significantly reduced the yield-scaled NH3 emissions, and the partial substitution of organic fertilizers for inorganic fertilizers should be recommended to promote environmental sustainability while ensuring food security.

In Vitro Antioxidant and Anticancer Potential of the Vegetative and Reproductive Organs of Embelia ruminata

ABSTRACT The antioxidant and anticancer potential of solvent extracts of the leaf, stem bark, fruit, and seeds of Embelia ruminata was investigated. The chloroform seed extracts had the highest TAC of 38.50 mg AAE g−1 DW. In the FRAP and DPPH assays, the methanolic seed extracts exhibited the most effective activity (67.63 mg AAE g−1 and IC50 of 3.54 µg mL−1, respectively), compared to the respective standards. In the MTT assays, the hexane seed extracts were the least cytotoxic and had a more pronounced effect on MCF-7 and A549 cell lines (IC50 of 93.1 and 119.5 µg mL−1, respectively) than the control.

Comparative transcriptome analysis of Cyperus esculentus and C. rotundus with contrasting oil contents in tubers defines genes and regulatory networks involved in oil accumulation

Plant vegetative organs present great potential for lipid storage, with tubers of Cyperus esculentus as a unique example. To investigate the genome and transcriptomic features of C. esculentus and related species, we sequenced and assembled the C. esculentus genome at the contig level. Through a comparative study of high-quality transcriptomes across 36 tissues from high-oil and intermediate-oil C. esculentus and low-oil Cyperus rotundus, we identified potential genes and regulatory networks related to tuber oil accumulation. First, we identified tuber-specific genes in two C. esculentus cultivars. Second, genes involved in fatty acid (FA) biosynthesis, triacylglycerol synthesis, and TAG packaging presented increased activity in the later stages of tuber development. Notably, tubers with high oil contents presented higher levels of these genes than those with intermediate oil contents did, whereas tubers with low oil contents presented minimal gene expression. Notably, a large fragment of the FA biosynthesis rate-limiting enzyme-encoding gene BCCP1 was missing from the C. rotundus transcript, which might be responsible for blocking FA biosynthesis in its tubers. WGCNA pinpointed a gene module linked to tuber oil accumulation, with a coexpression network involving the transcription factors WRI1, MYB4, and bHLH68. The ethylene-related genes in this module suggest a role for ethylene signaling in oil accumulation, which is supported by the finding that ethylene (ETH) treatment increases the oil content in C. esculentus tubers. This study identified potential genes and networks associated with tuber oil accumulation in C. esculentus, highlighting the role of specific genes, transcription factors, and ethylene signaling in this process.

Maize Responses to High Night Temperature During Postflowering and Early Grain Filling: Effects on Yield Components, Kernel Growth and Dry Matter Allocation

ABSTRACTWarm night frequency has increased steadily in the last years across maize production regions, but high night temperature (HNT) effects on growth, grain yield and maize dry matter allocation (DMA) to different plant organs remain poorly understood. In this study, we aimed to (i) analyse the DMA among reproductive and vegetative organs, (ii) evaluate the individual kernel weight through its determinants, rate and duration of grain filling and (iii) quantify changes in grain yield per plant and its components due to HNT during the postflowering and early grain‐filling period. Field‐grown maize was subjected to HNT induced by shelters during a 15‐ or 30‐day period after silking, encompassing the postflowering period (HNT15) and extending the heating into early grain filling (HNT30), respectively. The HNT was applied from 1900 to 0700 h while control plots remained at ambient night temperature (ANT). Kernel number per plant was decreased under both temperature regimes (i.e., HNT15 and HNT30); however, significant reductions in grain yield were only observed under HNT30. The DMA during the heating period was differentially affected by the duration of heating. While DMA to the stem was likewise reduced by both heating treatments, the partition to the uppermost ear was only reduced under HNT30. Related to the lack of response to HNT treatments of the rate and duration of grain filling, the individual kernel weight was not reduced. The source‐sink ratio was not affected by HNT, meanwhile, the apparent reserve use was significantly reduced under HNT30. Our results demonstrate that the magnitude of HNT effects is subjected to the duration of the heating period, but also depends on the intensity of heating explored across seasons, especially for kernel number and grain yield.

Young dove trees tend to invest more biomass in vegetative and reproductive organs than old trees at the twig level

Research on the functional traits of twigs is of great significance to clarify the plant life-history strategies, especially for endangered plants. However, little attention has been given to the environmental adaptation strategy of different age twigs. The dove tree (Davidia involucrata Baill.), a relict and critically endangered Chinese species, was employed as a model species. Total lamina mass and area, stem mass and length, total bract mass and area, and total petiole mass were measured for 100 current-year terminal twigs (50 per age group) from old and young dove trees in Pingwu County, Sichuan, southwest China. Then, the scaling relationships within the twig were determined using the standardized major axis (SMA) regression method. Our results showed that the significantly positive correlations among traits of D. involucrata twigs, and scaling relationships between these traits are different between the two age groups. At the twig level, isometric scaling relationships exist between stem mass and lamina size (or bract size) and between total lamina mass and total lamina area (or total petiole mass). In contrast, allometric scaling relationships exist between lamina size (total mass and area) and stem length (with a common slope > 1), and between total lamina mass and total petiole mass (with a common slope < 1). For a given stem mass, stem length and bract area, young trees have larger total lamina mass and total bract mass, larger total lamina mass and area, and larger total bract mass than old trees, respectively. The results suggest that young trees tend to have more vegetative and reproductive organ mass per unit of supporting organ mass than old trees to meet their rapid growth needs.

CHARACTERIZATION OF NEWLY INTRODUCED POMEGRANATE VARIETIES

The paper is focused on examining and selecting morphometric indicators of leaves and flowers from genotypes within the collection of the pomegranate varieties. The research was carried out on 6 varieties of pomegranate plants, collected and introduced from diverse ecological and geographical regions. The objective was to collect, cultivate and assess the technological features of pomegranate varieties and forms. The morphometric characteristics of generative and vegetative organs from the “Malas” and “Kirmizi Gazyan” varieties were studied for the first time. Observation of pomegranate flower petals revealed that different sizes could be found in both long toothed (LT), middle toothed (MT) and short-toothed (ShT) flowers, suggesting petal size is not determined by flower type. The difference between the leaves of these two varieties was found to be insignificant. Flower diameter showed variability across all three types of flowers. The biological and economic significance of the newly developed form F-6-8 and the varieties “Malta”, “Oleg” and “Devachi” was also assessed. “F-6-8” and “Malta” are sweet varieties, while “Oleg” and “Devachi” are sweet and sour varieties. “Malta” was distinguished by its fruit weight (380.4 g), thin skin (2.5 g) and high juice yield (50.4%).

Optimizing cotton growth in saline soil: Compound microbial agent modulates indigenous bacteria to enhance photosynthesis and vegetative-reproductive balance

Salt-tolerant composite plant growth-promoting rhizobacteria (STC-PGPR) can alleviate salt stress, though its complexity makes its stability and mechanisms in indeterminate-growing crops unclear. We tested STC-PGPR on two cotton (Gossypium hirsutum L.) varieties (G1: Xinluzao 72, G2: Zhongmiansuo 49), with two microbial treatments (B1: none, B2: with STC-PGPR) and three salinity levels (S1: 0 g NaCl kg−1, S2: 4 g NaCl kg−1, S3: 8 g NaCl kg−1). The pot experiment used a three-factor completely randomized design in a greenhouse, with NaCl mixed into the soil before transplanting. STC-PGPR (Paenibacillus polymyxa SC2, Bacillus velezensis DSYZ, Lactobacillus casei DY-3, and Saccharomyces cerevisiae DY-4) was applied 14 days post-transplant and during flowering and boll development. STC-PGPR was diluted with water and applied to the rhizosphere. Results showed that under S1, STC-PGPR increased G2’s seed cotton dry matter accumulation (Sc) by 25.1 %. Under S2, STC-PGPR increased G1’s boll dry matter accumulation (Bo) from 0.21 g to 7.7 g per pot. Other STC-PGPR treatments were less effective due to overdeveloped vegetative organs, highlighting the need for balanced vegetative and reproductive growth. High salinity reduced STC-PGPR effectiveness under S3. Random forest analysis identified crucial indicators regulated by STC-PGPR that significantly affected reproductive organ dry matter accumulation (P<0.001). Bioinformatics and correlation analyses showed biomarkers enriched by STC-PGPR influenced these indicators, not STC-PGPR directly. In S1, G2B2’s biomarkers enhanced stomatal conductance, increasing the net photosynthetic rate (Pn) and promoting dry matter accumulation. They also increased stem diameter and inhibited vegetative growth, enhancing transport and channeling energy to reproductive organs. In S2, G1B2’s biomarkers reduced leaf thickness, increased chlorophyll content to enhance Pn, and extended the maturation period. This enabled cotton to accumulate more photosynthetic products and allocate them to reproductive organs. This study demonstrates that STC-PGPR enhances productivity under salt stress, providing insights for optimizing saline soil cultivation.

Total polyphenol, flavonoid content and antioxidant activity of two species with ethnopharmaceutical uses from the spontaneous flora of the Oltenia region, Romania

In the conducted study, we investigated the total phenolic content of the alcoholic extracts from various vegetative organs of two plants of Romanian wild flora, Oltenia region: Dipsacus laciniatus and Armoracia rusticana. The antioxidant activities were determined by ferric reducing antioxidant power (FRAP), and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging methods. We also determined total polyphenol and flavonoid content. The results showed significant differences among plant various vegetative organs, the highest content of polyphenols being found for the leaves extract of Dipsacus laciniatus species, followed by Armoracia rusticana leaves. The highest antioxidant reducing power was obtained for the Armoracia leaf extract (IC50= 101.94 µg/ml), followed by the leaves of Dipsacus (IC50= 77.86 µg/ml).

Phenotypic characteristics of black apricot Prunus × dasycarpa cultivar ‘Shlor-Tsiran’ in Armenia

ABSTRACT ‘Shlor-Tsiran’ (literally called ‘Plum-Apricot’) is an old black apricot cultivar, which is traditionally cultivated in Armenia. Taxonomically, it belongs to Prunus × dasycarpa Ehrh., which is a spontaneous hybrid of apricot and cherry plum (P. armeniaca L. × P. cerasifera Ehrh.). Currently, ‘Shlor-Tsiran’ is considered to be very rare in Armenia and it occurs as single trees in old home orchards of the Ararat Valley. It is characterised by high frost and disease resistance, late flowering and fruiting compared with other cultivated apricots, and is of interest for the development of apricot cultivars displaying delayed fruit ripening and enhanced frost resistance. This study aims to phenotype the cv. ‘Shlor-Tsiran’ taking into consideration 67 quantitative and qualitative characteristics of vegetative and generative organs, growth and development. Morphological description of the cv. ‘Shlor-Tsiran’, comparing with its parent species, P. armeniaca and P. cerasifera, was performed. Data on fertility and viability of pollen grains, biochemical and nutritional properties of fruits and tree productivity are provided. The data obtained should contribute to a wider use and conservation of the black apricot cv. ‘Shlor-Tsiran’ gene pool, which can be used for breeding purposes as well. The article is illustrated with tables and original photographs.

The anatomy, micromorphology, and essential oils of the Turkish endemic and endangered species Alchemilla orduensis

In this study, the anatomical and micromorphological characteristics of the vegetative organs and the essential oil constituents of the aerial and underground parts of the local and endangered endemic species A. orduensis Pawł. were evaluated. For anatomical study, sections of root, rhizome, stem, leaves and petiole were excised and stained with safranin/fast green mixture. Leaf and petiole structures were examined micromorphologically. Essential oil contents were determined by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME/GC-MS) analysis. The results showed that rectangular meristematic cells were present in the root. The leaf is of the bifacial and amphistomatic type. Stomata cells are of the anomocytic type. The stomatal index for the upper surface of the leaves is 0.04, while the stomatal index for the lower surface is 0.17. Druse crystals were found in the rhizome, stem and leaves. Among the various compounds identified, the most abundant groups in the aboveground parts are alcohols (39.81%) and ketones (14.99%) with 1-Octen-3-ol, 1-octan-3-one and borane- methyl sulfide complex as the main compounds. Terpenes (23.44%) and alcohols (11.82%), in which myrtenolis was the main compound, were most abundant in the underground parts.

Source-sink relationships during grain filling in wheat in response to various temperature, water deficit, and nitrogen deficit regimes.

Grain filling is a critical process for improving crop production under adverse conditions caused by climate change. Here, using a quantitative method, we quantified post-anthesis source-sink relationships of a large dataset to assess the contribution of remobilized pre-anthesis assimilates to grain growth for both biomass and nitrogen. The dataset came from 13 years of semi-controlled field experimentation, in which six bread wheat genotypes were grown at plot scale under contrasting temperature, water, and nitrogen regimes. On average, grain biomass was ~10% higher than post-anthesis above-ground biomass accumulation across regimes and genotypes. Overall, the estimated relative contribution (%) of remobilized assimilates to grain biomass became increasingly significant with increasing stress intensity, ranging from virtually nil to 100%. This percentage was altered more by water and nitrogen regimes than by temperature, indicating the greater impact of water or nitrogen regimes relative to high temperatures under our experimental conditions. Relationships between grain nitrogen demand and post-anthesis nitrogen uptake were generally insensitive to environmental conditions, as there was always significant remobilization of nitrogen from vegetative organs, which helped to stabilize the amount of grain nitrogen. Moreover, variations in the relative contribution of remobilized assimilates with environmental variables were genotype dependent. Our analysis provides an overall picture of post-anthesis source-sink relationships and pre-anthesis assimilate contributions to grain filling across (non-)environmental factors, and highlights that designing wheat adaptation to climate change should account for complex multifactor interactions.

Is urbanization a driver of aboveground biomass allocation in a widespread tropical shrub, Turnera subulata (Turneroideae - Passifloraceae)?

Plant biomass allocation is mainly affected by the environment where each individual grows. In this sense, through the rapid global expansion of impermeable areas, urbanization has strong, albeit poorly understood, consequences on the biomass allocation of plants found in this environment. Nevertheless, the comprehension of biomass allocation processes in urban shrubs remains unclear, because most studies of urban ecology focus on tree species. This is an important gap of knowledge because a great part of urban vegetation is composed of shrubs and their association with trees have positive impacts in urban ecosystem services. In this study, we explored the ecological and potential selective pressure effects of an urbanization gradient on the biomass allocation patterns of aboveground organs of Turnera subulata, a widely distributed tropical shrub. We have demonstrated that, for certain reproductive organs, biomass allocation decreases in locations with higher urbanization. Unlike expected, the biomass of vegetative organs was not affected by urbanization, and we did not observe any effect of urbanization intensity on the variance in biomass allocation to vegetative and reproductive organs. We did not record urbanization-mediated trade-offs in biomass allocation for reproductive and vegetative organs. Instead, the biomass of these structures showed a positive relationship. Our data suggest that urbanization does not result in radical changes in biomass allocation of T. subulata, and neither in the variation of these traits. They indicate that the ability of T. subulata to thrive in urban environments may be associated with life history and morphological mechanisms. Our findings contribute to the understanding of shrub plant responses to urbanization and highlight urbanization as a potential factor in resource allocation differences for different structures and functions in plants living in these environments.

Genome-wide identified VvOFP genes family and VvOFP4 functional characterization provide insight into fruit shape in grape

Ovate Family Proteins (OFPs) are emerging as novel transcriptional regulators of fruit shape. Despite their established role in various species, their involvement in regulating grape fruit shape remains understudied. This study encompassed a comprehensive evaluation of 16 grape OFP genes in total at the whole genome level. Phylogenetic and synteny analyses established a close relationship between grape VvOFP genes and their tomato counterparts. Expression profiling post-treatment with gibberellic acid (GA3) and thidiazuron (TDZ) revealed that certain OFP genes responded to these regulators, with VvOFP4 showing peak expression three days post-anthesis. Functional assays via overexpression of VvOFP4 in tobacco and tomato altered the morphology of both vegetative and reproductive organs, including leaves, stamens, and fruits/pods. Paraffin sections of transgenic tobacco stems and tomato fruits demonstrated that VvOFP4 overexpression modifies cell dimensions, leading to changes in organ morphology. Additionally, treatments with GA3 and TDZ similarly influenced the shape of grape pulp cells and thereby the overall fruit morphology. These findings suggest that the VvOFP4 gene plays a crucial role in fruit shape determination by modulating cell shape and presents a potential target for future grape breeding programs aimed at diversifying fruit shapes.

Chilling at grain filling stage reduced rice grain protein content: An experimental and modeling study

Climate change has increased the trend in the intensity of global extreme weather events, including chilling. Nitrogen is one of the most essential nutrients for rice growth and development. Chilling will limit the uptake and translocation of nitrogen and the formation of grain protein in rice plants. The experiment conducted in the climate chamber with chilling stress applied at the grain filling stage revealed different effects on protein concentration in aboveground organs and caused asymmetry in grain protein contents (GPC) and grain protein yield (GPY). Chilling stress applied during the grain filling stage reduced the accumulation rate of rice grain protein by inhibiting the nitrogen uptake and the translocation from vegetative organs to grains, which resulted in a 35 % decrease in grain nitrogen accumulation and a 92 % increase in vegetative organs during grain filling. Consequently, the average nitrogen harvest index is reduced by 12 %. Nitrogen accumulation was severely affected when cooling degree days (CDD) ≥ 70 °C days. As the chilling intensity increased, the decrease of GPY was more significant than that of GPC. Moreover, we improved the grain temperature-nitrogen uptake function under chilling stress based on the relationship between CDD and the reduction in rice grains. By comparing the improved function and the modules in the existing crop model using the datasets from open field and artificial control experiments, we demonstrated that the current research on quantifying rice nitrogen uptake at extreme temperature stress needed further improvement. The effects of environmental stress on grain nitrogen accumulation are complex. Future studies should pay more attention to the ability of extreme temperature stress to affect nitrogen accumulation in various rice organs.