Grapes, the second next to Citrus, are one of Egypt's most popular fruits. Egypt is the world's 14th largest grape producers. The Flame seedless grape is a popular and early-season cultivar and one of the most prevalent grape kinds. Because the grape is nematode-sensitive, grafting on nematode-resistant rootstocks could be an effective way to minimize worm infection. The purpose of this study is to investigate the effect of IBA, Kinetin in graft union, and some grapevine rootstocks, such as Harmony, Freedom, and Salt Creek, on the percentage of Flame seedless grapevine survival and vegetative growth parameters when grafted using the cleft grafting method on the 15th February grafting date. When the Flame seedless grapes were treated at the graft union of the Freedom rootstock with a high level of indole-3-butyric acid (IBA, 1000 ppm) and a low level of Kinetin (250 ppm), they had a significantly higher survival percentage, shoot length, shoot diameter, L/D ratio, number of leaves per shoot, leaf area, fresh weight of leaves, fresh weight root system, dry weight of leaves and shoots, and dry weight root system in both seasons. It can be concluded that grafting on Freedom rootstock and using a low amount of Kinetin (250 ppm) are the most effective treatments for getting the highest percentage of survival and vegetative development parameters in Flame seedless grapevine.