Background: environmental pollution has led to climate change and agriculture is an aspect of this pollution, Pesticides, fertilizers and plant growth regulators are pollutants in soil and water. Therefore, beneficial microorganisms found in nature to provide plants with nutrients, resist pests and reduce mineral fertilizers is an ambitious goal for researchers. On the environment. Pseudomonas bacteria, baker’s yeast and mycorrhizal fungi are known for their ability to increase a group of nutrients’ organic and amino acids, as well as plant hormones that stimulate growth. Methods: A field experiment was conducted at University of Baghdad to assess the impact of beneficial microorganisms on wheat growth traits during winter Of 2019-2020., using a randomized complete block design (RCBD) with eight treatments.: Pseudomonas fluorescens bacteria (Pse), Mycorrhizae Glomusspp. (Gl.), Saccharomyces cerevisiae yeast (Sa.), Ps+Gl, Gl+Sa, Ps+Sa, Ps+Sa+Gl and control. Result: Experimental indicated that treatments significantly outperformed in plant height, tiller number, CGR (crop growth rate) and total dry weight. Additionally, the Ps+Sa+Gl treatment demonstrated a significant superiority over all other treatments in terms of plant height and tiller number. These observations suggest that the combination of biological factors within a single treatment resulted in a synergistic interaction, that led to enhancement of the vegetative growth indices in wheat plants.