Vegetables In Kenya Research Articles

The effects of market-oriented farming on living standards, nutrition, and informal sharing arrangements of smallholder farmers: the case of African indigenous vegetables in Kenya

AbstractThe shift from subsistence to more market-oriented agriculture is viewed as essential to increase smallholder farmers’ welfare. However, its impact on farmers’ nutrition and informal sharing arrangements and associated solidarity within African farming communities remains uncertain. To analyse these trade-offs, we study the growing commercialization of African indigenous vegetables (AIV) in Kenya. These vegetables are an essential component of local diets in rural areas but also of informal sharing arrangements that provide access to food outside of markets. This article combines quantitative data from a 2016–2022 panel survey of farmers with qualitative data from focus group discussions. Results based on household fixed-effects models show a significant increase in households’ non-food expenditures due to selling AIV. The results suggest that selling AIV did not negatively affect nutrition outcomes but did not improve them either. Informal AIV sharing between households decreased further with growing market participation. Panel data models indicate, however, inconsistent and insignificant changes in associated solidarity indicators. We attribute this to the multiple and sometimes opposing effects of market-oriented farming on solidarity, as revealed by focus group discussions. While some farmers perceive reduced solidarity due to less informal AIV sharing, others perceived this traditional solidarity to be partially forced. Other forms of social interaction have also emerged, such as cooperatives and more intensive knowledge sharing. Despite concerns about the loss of informal sharing and community solidarity and limited improvements in nutrition outcomes, the tangible income gains generated by selling AIV are likely to foster further growth in the AIV sector.

Effect of blanching time-temperature on potassium and vitamin retention/loss in kale and spinach.

Hyperkalemia is common among patients with end stage kidney disease. Management involves diet modification. Hot water blanching is recommended to leach potassium in vegetables which results in losses of water-soluble and heat labile vitamins. Evidence on the effect of blanching in reducing potassium level of locally consumed vegetables in Kenya is limited. This study sought to establish effect of hot water blanching time-temperature on level of potassium, vitamin B1, B3 and C in kales (Brassica oleracea var. acephala) and spinach (Spinach oleracea) on potassium and vitamins B1, B3 and C retention/loss. The study adopted a full factorial experimental design. Vitamins were determined using high performance liquid chromatography. Potassium was quantified using atomic absorption spectrophotometry. To compare nutrient content between samples, independent t-test and Analysis of Variance were used at 95% confidence level. Nutrient content of fresh kales and spinach were potassium (102 mg/100 g and 615 mg/100 g), vitamin B1 (124 μg/100 g and 51 μg/100 g), vitamin B3 (1165 μg/100 g and 812 μg/100 g) and vitamin C (102 mg/100 g and 116 mg/100 g) respectively. In kales, blanching for 20 min at 1000°C resulted to retention of 86.9%, 55.6%, 27.6% and 12.9% of vitamin B1, B3, C and potassium respectively. In spinach, blanching for 20 min at 1000°C resulted in retention of 79.9%, 88.6%, 12.2% and 40.6% retention of vitamin B1, B3, C and potassium respectively. Vitamin C and Potassium were the most sensitive to heat and leaching. Time had a greater effect than temperature in this study. This study recommends blanching of kale at 15.2 min at 800°C, spinach at 17.7 min at 840°C. Further research on optimal blanching time-temperature for potassium and vitamin retention/loss is recommended.

Impacts of Climate Change on Vegetation in Kenya: Future Projections and Implications for Protected Areas

Climate change will cause substantial vegetation shifts across the world. Africa may face varying dynamics such as tree decline, savannization, and woody encroachment due to rising temperatures and rainfall changes. This study examines the potential effects of climate change on Kenyan vegetation and vegetation shifts for 2050 and 2100, employing a statistical model to predict vegetation state as driven by environmental variables, including temperature, soil moisture, livestock density, and topography. We evaluate the model by hindcasting it from 2020 to 2000 and then project future vegetation states for 2050 and 2100 under SSP 2–4.5 and SSP 5–8.5. In response to moderate emissions, a notable increase in arid-associated shrubland vegetation (53–58%) is forecasted, leading to the expansion of drylands at the expense of savannas, grasslands, and forests. Under high-emission scenarios, savannas are forecasted to expand (52–65%) at the expense of forested areas. Overall, dense forest cover declines across scenarios, affecting protected areas by promoting increased savanna cover and reducing forest area (40% to 50%). These projected shifts in major vegetation types would likely alter ecosystem functioning and associated services, impacting pastoralists and wildlife and raising biodiversity concerns. Protected areas in Kenya could lose 50% of their forests, highlighting the urgency of climate change mitigation. These findings offer a crucial foundation for future research and action on Kenya’s vegetation.

Isolation and Identification of Autochthonous Lactic Acid Bacteria from Commonly Consumed African Indigenous Leafy Vegetables in Kenya

African indigenous leafy vegetables (AILVs) are plants that have been part of the food systems in Sub-Saharan Africa (SSA) for a long time and their leaves, young shoots, flowers, fruits and seeds, stems, tubers, and roots are consumed. These vegetables are high in vitamins, minerals, protein, and secondary metabolites that promote health. This study aimed at isolating, characterizing, and identifying dominant lactic acid bacteria (LAB) from naturally fermenting commonly consumed AILV in Kenya. A total of 57 LAB strains were isolated and identified based on phenotypic and 16S rRNA gene analyses from three AILVs (23 nightshade leaves, 19 cowpeas leaves, and 15 vegetable amaranth). The highest microbial counts were recorded between 48 h and 96 h of fermentation in all AILVs ranging from approximately log 8 to log 9 CFU/mL with an average pH of 3.7. Fermentation of AILVs was dominated by twenty eight Lactobacillus spp. [Lactiplantibacillus plantarum (22), Limosilactobacillus fermentum (3), Lactiplantibacillus pentosus (2) and Lactiplantibacillus casei (1)], eleven Weissella spp. (Weissella cibaria (8), W. confusa (2), and W. muntiaci) six Leuconostoc spp. [Leuconostoc mesenteroides (3), Leuc. citreum (2) and Leuc. lactis (1)], six Pediococcus pentosaceus, four Enterococcus spp. [Enterococcus mundtii (2), E. faecalis (1) and E. durans (1)] and, finally, two Lactococcus garvieae. These bacteria strains are commonly used in food fermentation as starter cultures and as potential probiotics.

Effect of harvest stage and nitrogen fertilization on the postharvest shelf life of black nightshade (Solanum nigrum L.) and collard (Brassica oleracea var. acephala L.)

Leafy vegetables play a crucial role in the human diet providing numerous nutrients and health benefiting compounds. Leafy vegetables like collard (Brassica oleracea var. acephala) and black nightshade (Solanum nigrum l.) are commonly consumed leafy vegetables in Kenya. However, their high perishability and short shelf life (usually 1-2 days at ambient temperature) limits their utilization resulting in significant high postharvest losses. This study assessed the effect of harvest stage and nitrogen fertilization on the postharvest shelf life of collard and black nightshade. Experiments were conducted at Kabete field station, University of Nairobi, using collard and black nightshade. Field experimental layout was a 4 x 3 factorial arrangement in randomized complete block design with three replicates. Factors were nitrogen levels and harvest stage. Four levels of nitrogen (0, 30, 60 and 90 kg N/ha) were applied on black nightshade and (0, 55.5, 111.1 and 166.6 kg N/ha) in collard where 0 kg N/ha was the control. Collard and black night shade were harvested at three harvest stages: 4 weeks, 6 weeks and 8 weeks after transplanting. The harvested vegetables were kept at ambient room condition (20 ºC, 55% relative humidity). Data collection was performed daily for quality related parameters which included color change, wilting index and cumulative weight loss. Results show that there was a progressive deterioration in quality of the collard and black nightshade with storage time regardless of harvest stage and nitrogen level. Harvesting at 8 weeks after transplanting resulted in longer shelf life in collard (three days) and black nightshade (two days) when compared to harvesting at 4 weeks or 6 weeks after transplanting. Collard and black nightshade showed reduced hue angles over storage time at different harvest stages. Black nightshade subjected to 90 kg N/ha and harvested at 4 weeks after transplanting had the highest wilting index of 33%. The highest cumulative weight loss of 29% was recorded in collard that were harvested at 8 weeks after transplanting. Black nightshade subjected to 90 kg N/ha and harvested at 6 weeks after transplanting showed the best color at a hue angle of 145°. Overall, harvesting at 8 weeks after transplanting resulted in the longest shelf life of both black nightshade and collard. These results show that low application of nitrogen fertilizer in black nightshade (30 kg N/ha) and in collard (55.5 kg N/ha) had minimal effects on weight loss and wilting and resulted in good keeping quality. Key words: Black nightshade, Collard, Harvest Stage, Nitrogen nutrition, Shelf life

Exploring the Status of Preference, Utilization Practices, and Challenges to Consumption of Amaranth in Kenya and Tanzania.

African leafy vegetables such as amaranth have been utilized since time immemorial both as food and as medicine. These vegetables grew naturally in most rural environments, but currently most of them are cultivated both for home consumption and for sale. The aim of this study was to identify the most preferred amaranth species and cooking and utilization practices, as well as the beliefs and attitudes that encourage or discourage use of this vegetable. The study was carried out in seven counties of Kenya and in three regions in Tanzania. Twenty Focus Group Discussions (FGDs) with members of the community and twenty Key Informant Interviews (KIIs) with agricultural and nutrition officers were conducted in the study areas to obtain information on preferred varieties, sources of amaranth vegetables, common cooking methods, alternative uses, beliefs and taboos surrounding amaranth consumption, and the challenges experienced in production and consumption. The findings of the study showed that amaranth is one of the most commonly consumed indigenous vegetables in Kenya and Tanzania. The preference for varieties and cooking habits differs depending on the community and individuals. Amaranthus dubius and Amaranthus blitum were most common in Kenya, while Amaranthus dubius and Amaranthus hypochondriacus were most common in Tanzania. Most people consumed these vegetables because they were affordable and available or because of circumstance of lacking other foods. Regarding cooking, final taste was mostly considered rather than nutritional attribute. Several alternative uses of amaranth such as uses as medicine and livestock feed were also reported, as well as some beliefs and taboos surrounding the vegetable. Training on nutritional attributes and promotion of food preparation practices that ensure maximum nutrient benefits from amaranth is needed at the community level to realize the nutritional importance of the vegetables. Hands-on training and demonstrations were the most preferred modes of passing information.

Factors influencing entrepreneurial orientation levels among agri-input suppliers in Nakuru county, Kenya

Start-ups and small-scale agri-input suppliers (AIS) play critical roles in Kenya’s agricultural development sector. Nakuru county is the leading producer of flowers, potatoes, milk and vegetables in Kenya; creating an enabling environment for AIS start-upsto thrive. However, competition from established, medium and large-scale agri-enterprises put them at risk of closure or inhibition of their potential to survive the environmental turbulence and grow into medium or large-scale enterprises.Entrepreneurial orientation (EO) manifestations, in the form of proactiveness, innovation, and risk-taking, among other internal aspects, are considered necessary for any agri-enterprises’ start-up success, as they positively influence their growth and performance. The objective of this study was to understand factors influencing EO levels among agri-input suppliers in Nakuru County, Kenya.A study involving a sample of 137 agri-input suppliers operating in Nakuru County was carried out using face-to-face semi-structured questionnaire. Data collected were analysed using Principal Component Analysis, Quartile technique, and the Generalised Ordered Logit (GOL) model. Overall, the variety of traded agri-input products showed the most significant positive influence on EO levels, followed by possession of business plan, partnership, customers’ contracts and gender factors, in that order of magnitude. On the other hand, years ofagri-enterprise operation and the number of agri-input business owners showed negative influence on EO levels. The list of prioritised significant factors is important in informing agripreneurs, policy makers and socio-economic development agencies when designing development programmes and strategies aimed at promoting agripreneurship in Kenya.

Synthesizing social and environmental sensing to monitor the impact of large-scale infrastructure development

The booming development of large-scale infrastructure projects (LSIPs) facilitated by China’s Belt and Road Initiative (BRI) has drawn global concern regarding the scale, pace, and potential impact. Studies have largely focused on the geopolitical impact (i.e., politics and international relations) but less is known about social and environmental impact. This is in large part because consistent, high-resolution, cross-boundary social and environmental data at large scales are rather limited. To address the knowledge gap, this research developed a novel Socio-Environmental Sensing (SES) approach by synthesizing remote sensing imagery and geotagged Twitter data to map the socio-environmental impact of LSIPs. We demonstrated the applicability of this approach using two BRI flagship projects, namely, the Mombasa-Nairobi Standard Gauge Railway (SGR) in Kenya and the China-Pakistan Economic Corridor (CPEC) in Pakistan. Our analysis shows that both projects have led to a substantial loss of natural land (e.g., 3.7 % loss of vegetation in Kenya, and 23.3 % reduction of the glacier in Pakistan) but gains in artificial land (e.g., 4.2 % increase in cropland in Kenya, and 34.6 % expansion of built-up land in Pakistan). In addition, the BRI-LSIPs have largely improved local economic development, because nighttime light imagery revealed that regions near the BRI-LSIP sites became much brighter than other regions. Regarding the social aspect, we found that public sentiment toward the projects was largely positive and improved over time, which contradicts the prevalent pessimism to BRI-LSIPs by critics. Nevertheless, sentiment also presented strong spatial heterogeneity – regions around the BRI transportation hubs (usually large cities) most showed more positive sentiment than other regions. By spatially joining the georeferenced sentiment scores with environmental indicators from remote sensing, we further found that positive sentiment improved more in more developed regions, but only changed slightly in other regions. This study provides a novel approach to assess the socio-environmental impact of large-scale projects, and the findings would be useful for informing the implementation of future BRI projects across the globe.

AN EVALUATION OF THE FACTORS INFLUENCING VEGETABLE COMMERCIALIZATION IN KENYA

Purpose: The purpose of the study was to establish factors that influence vegetable commercialization among smallholder farmers of fresh vegetables in Kenya. The present study defined commercialization against farmer characteristics including portion of land committed to vegetable farming, share of household income generated from it, and the producers’ perception that the vegetables they consumed at the farm were produced or procured without any substantial cost.
 Methodology: The researcher adopted a cross-sectional survey design to randomly recruit a sample of crop farmers (n=118) from Kirinyaga and Kiambu counties in Kenya. Data on agrochemical use, crop diversity and scale as well as logistical issues regarding proximity to markets and social status of the crop farmers were collected using a researcher-administered questionnaire in a one-on-one interview. These data were initially entered on MS Excel spread sheets for cleaning and later transferred to Minitab (Ver 12) for statistical analyses. Principal component analysis (PCA) was used to isolate crucial commercialization factors responsible for varying agrochemical use behaviours among crop farmers and later presented in scree plots.
 Results/Findings: The study revealed that commercialization of tomatoes and kale was real in different areas of study which was influenced by structural and socio-demographic factors. From the study findings, structural policies to promote horticulture and high value crop production had a positive impact on production of vegetables while wildlife conservation and livestock development resulted to human-wildlife conflicts due to agrochemical abuse. Various socio-demographic factors that influence commercialization of vegetables included: household size, farm size, proportion of land used for vegetable production and household income. It emerged that household size (Eigen value EV=3.63), income (EV=2.61) and farm size (EV=1.27) accounted for 68.4% of variability in agrochemical abuse. This finding also explains their marginalization from county government extension services and high costs of market access.
 Unique contribution to theories, practice and policy: The study finds that vegetable farmers’ perception of commercialization leaned more towards increasing production in response to consumer demand and not necessarily to raise household food supplies. Famers dedicated over two thirds (66.67%) of their land to vegetable farming to support their livelihood (97.1%, SE= 1.30). Commercialization of vegetable farming was influenced by the level of education (SE=3.68). The study found out that that access to ICT equipment, particularly mobile phones, were the prime means via which farming techniques and agribusiness practices were learnt or improved (V= 0.196 Pearson, p=0.11).

Organophosphate and carbamate pesticide residues and accompanying risks in commonly consumed vegetables in Kenya

ABSTRACT The current study was conducted to assess the levels of organophosphates and carbamates in vegetables in Kenya and to examine potential consumer health risks. A total of 90 samples were analysed by liquid chromatography/high-resolution tandem mass spectrometry. Residues of acephate, chlorpyrifos, methamidophos, omethoate and profenofos were found in 22% of the samples, ranging from 10 to 1343 μg/kg. The EU MRL was exceeded in 21%, 10%, 8% and 22% of the samples of French beans, kales, spinach and tomatoes, respectively. Chlorpyrifos in spinach had an acute HQ of 3.3 and 2.2 for children and adults, respectively, implying that potential health risks with respect to acute dietary exposure cannot be excluded. For chronic dietary exposure, all chronic HQs were below 1. The HI for the pesticides was 0.54 and 0.34 for children and adults. Routine monitoring of OPs and carbamates in vegetables is recommended to minimise consumer’s health risks.

Agro-Morphological Characterization of Kenyan Slender Leaf (Crotalaria brevidens and C. ochroleuca) Accessions

Slender leaf (Crotalaria spp) is among the indigenous and underutilized vegetables in Kenya whose production is limited to the Western and Coastal regions of the country. For a long time, this crop has been neglected in terms of research and genetic improvement. There is therefore scanty information on its morphological diversity and agronomic performance, hence the need for this study. Field experiments were carried out for two seasons in October to December 2018 and March to May 2019. The experiments were laid out in Randomized Complete Block Design with 29 accessions and replicated three times. Both qualitative and quantitative data were recorded from the accessions based on the Crotalaria descriptors. Quantitative data were subjected to analysis of variance using XLSTAT Version 2019, and accession means were separated using Student’s Newman Keuls test at 95% level of confidence. Both qualitative and quantitative data were subjected to multivariate cluster analysis, and a dendrogram was constructed using the unweighted pair-group method with arithmetic average. The principal component analysis was conducted to obtain information on the importance of the characters. Significant variation in agro-morphological traits was found within and between the two species. Cluster analysis grouped the accessions into seven major classes with a between-classes diversity of 75.13% and a within-classes diversity of 24.87%. This study sets the basis for genetic improvement of slender leaf in Kenya since the observed diversity can be exploited in selection for intraspecific and interspecific hybridization.

Farmers’ knowledge, attitudes and practices (KAP) on production of African indigenous vegetables in Kenya

Local knowledge, attitudes, and practices (KAP) regarding African Indigenous Vegetables (AIVs) among local growers are required to design effective vegetable productivity-enhancing interventions. However, limited studies have been conducted in Kenya to assess the knowledge, attitudes, and practices on production performance of AIVs. A survey was conducted among 600 AIV growers in Busia, Nyamira and Machakos Counties of Kenya to assess the knowledge, attitudes and practices regarding AIVs and their contribution on output/yield. Primary data were collected using a semi-structured questionnaire supplemented with key informant interviews. Results showed significant knowledge and positive attitude of farmers regarding AIVs, but insignificant use of improved farming practices. The study used a binary logistic regression to identify the variables that are associated with yield increase in AIVs production. It was established that by employing a 5% criterion of statistical significance, ploughing tools, land proportion allotted to the cultivation of AIVs, and water irrigation in AIVs farming significantly influenced the output/yield. Whereas the agro-ecological zones, gender of the farmer, and the fact of being trained in AIVs farming were statistically significant at 10%. On the other hand, farmers’ KAP was not statistically significant. An important implication of this result is that farmers’ personal characteristics need to be taken into consideration when planning developmental programmes for them. We encourage further intervention studies to be done to complement these findings in order to enable effective methods of interventions to boost production of AIVs.

Gaining market insights for aquaponically produced vegetables in Kenya

Aquaponics is a promising technique which enables continuous pesticide-free food production in arid regions. Substantial research has been done with respect to aquaponics technologies and production. However, the demand side of the market has not been investigated in much detail yet. This exploratory study analysed the Nairobian market for aquaponically produced foods. Interviews provided data to get some first insights into the market prices and customers’ perception of aquaponically produced food products. Results showed that the majority of the interviewees claim to be willing to pay more for aquaponically produced food products.

Field and Laboratory Performance of False Codling Moth, Thaumatotibia Leucotreta (Lepidoptera: Troticidae) on Orange and Selected Vegetables.

False codling moth (FCM), Thaumatotibia leucotreta is a key pest of citrus orange and other plants causing fruit loss through larval feeding. Although this pest is native to sub-Saharan Africa little is known on its performance on orange and vegetables in Kenya and Tanzania. Our objective was to assess the incidence, oviposition preference and offspring performance of FCM on orange and vegetables, namely, okra, African eggplant, chili and sweet peppers. A higher percentage of orange with FCM damage symptoms was recorded from the ground than from the tree sampled fruit. However, FCM larval incidence was higher for the latter (tree sampled fruit). The highest FCM larval incidence amongst the vegetables was recorded on African eggplant (12%) while the lowest was on okra (3%). Orange was the most while African eggplant was the least preferred for oviposition by FCM. Among the vegetables tested, strong oviposition preference was found for sweet pepper; however, larval survival was lowest (62%) on this crop. Highest larval survival (77%) was recorded on orange. Most demographic parameters (i.e., intrinsic rate of increase, doubling time) were comparable among the studied host plants. The results are discussed in line of FCM management.

Welfare and food security effects of commercializing African indigenous vegetables in Kenya

African indigenous vegetables (AIVs) have high nutritional value, forming a potent weapon against the pressing hidden hunger problem in East Africa, but they are not sufficiently adopted as cash cr...

Social Networks and Commercialisation of African Indigenous Vegetables in Kenya: A Cragg’s Double Hurdle Approach

This paper employs a two-stage Cragg’s double-hurdle model to assess the effects of market information networks on commercialisation decisions of smallholders of African Indigenous Vegetables (AIVs...

Effect of Global-GAP Policy on Climate Change Perceptions of Smallholder French Beans Farmers in Central and Eastern Regions, Kenya

The risks posed by climate change to Sub Saharan Africa’s (SSA) smallholder fresh export fruit and vegetables production are amplifying the significance of farmers’ climate change perceptions in enhancing adoption of suitable adaptation strategies. Production of fresh export fruit and vegetables in Kenya has increasingly been done under the Global-GAP standard scheme by smallholder farmers to improve both environmental conservation and market access. The objective of this study was to determine the effect of Global-GAP policy on climate change perceptions of smallholder French beans farmers. The analysis was based on data collected from a random sample of 616 households interviewed in the Central and Eastern regions of Kenya. The study used principal component analysis (PCA) to extract farmers’ key prevailing climate change perceptions and logit regression model to examine the effect of Global-GAP policy on climate change perceptions among other socio-economic factors. The PCA analysis extracted three components proxying for ‘droughts’, ‘delay in rainy seasons’, ‘diseases and pests’ and three proxying for ‘hot days’, ‘floods’, and ‘diseases and pests’ as summarizing maximum variance in the perceptions in the Central and Eastern region respectively. The common, study area-wide climate change perception was identified as incidence of diseases and pest. Logit regression analysis found that Global-GAP policy significantly influenced and improved farmers’ probability of perceiving climate change. Other factors found to influence farmers’ probability of having the identified climate change perceptions included regional specificity, access to agricultural extension service, access to credit, plot size, and soil fertility. The policy implication of this study is that the government and service providers should mainstream factors like Global-GAP compliance and regional considerations found to improve probability of perceiving climate change in awareness creation extension strategies, towards enhancing adoption of adaptation measures in the smallholder fruits and vegetables farming sector.

Meal Cultures - A New Concept in Food Security Debates on African Leafy Vegetables in Kenya and East Africa

Meal Cultures - A New Concept in Food Security Debates on African Leafy Vegetables in Kenya and East Africa

Matrix-assisted laser desorption/ionization time of flight mass spectrometry for comprehensive indexing of East African ixodid tick species.

BackgroundThe tick population of Africa includes several important genera belonging to the family Ixodidae. Many of these ticks are vectors of protozoan and rickettsial pathogens including Theileria parva that causes East Coast fever, a debilitating cattle disease endemic to eastern, central and southern Africa. Effective surveillance of tick-borne pathogens depends on accurate identification and mapping of their tick vectors. A simple and reproducible technique for rapid and reliable differentiation of large numbers of closely related field-collected ticks, which are often difficult and tedious to discriminate purely by morphology, will be an essential component of this strategy. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is increasingly becoming a useful tool in arthropod identification and has the potential to overcome the limitations of classical morphology-based species identification. In this study, we applied MALDI-TOF MS to a collection of laboratory and field ticks found in Eastern Africa. The objective was to determine the utility of this proteomic tool for reliable species identification of closely related afrotropical ticks.MethodsA total of 398 ixodid ticks from laboratory maintained colonies, extracted from the hides of animals or systematically collected from vegetation in Kenya, Sudan and Zimbabwe were analyzed in the present investigation. The cytochrome c oxidase I (COI) genes from 33 specimens were sequenced to confirm the tentatively assigned specimen taxa identity on the basis of morphological analyses. Subsequently, the legs of ticks were homogenized and analyzed by MALDI-TOF MS. A collection of reference mass spectra, based on the mass profiles of four individual ticks per species, was developed and deposited in the spectral database SARAMIS™. The ability of these superspectra (SSp.) to identify and reliably validate a set of ticks was demonstrated using the remaining individual 333 ticks.ResultsUltimately, ten different tick species within the genera Amblyomma, Hyalomma, Rhipicephalus and Rhipicephalus (Boophilus) based on molecular COI typing and morphology were included into the study analysis. The robustness of the 12 distinct SSp. developed here proved to be very high, with 319 out of 333 ticks used for validation identified correctly at species level. Moreover, these novel SSp. allowed for diagnostic specificity of 99.7 %. The failure of species identification for 14 ticks was directly linked to low quality mass spectra, most likely due to poor specimen quality that was received in the laboratory before sample preparation.ConclusionsOur results are consistent with earlier studies demonstrating the potential of MALDI-TOF MS as a reliable tool for differentiating ticks originating from the field, especially females that are difficult to identify after blood feeding. This work provides further evidence of the utility of MALDI-TOF MS to identify morphologically and genetically highly similar tick species and indicates the potential of this tool for large-scale monitoring of tick populations, species distributions and host preferences.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1424-6) contains supplementary material, which is available to authorized users.

Assessment of Spider Plant (Cleome gynandra L.) Germplasm for Agronomic Traits in Vegetable and Seed Production: A Green House Study

Aims: Spider plant ( Cleome gynandra L.) is one of the most important traditional vegetables in Kenya, albeit underutilized. Concurrently, scanty information is available on its potential for vegetable and seed production. The objective of this study was therefore to evaluate spider plant germplasm for agronomic traits pertinent to seed and vegetable production. Study Design: A complete randomized design (CRD) with three replicates was used. Place and Duration of Study: Two greenhouse experiments were conducted at Upper Kabete Field Station of the University of Nairobi, Kenya, for a period of 180 days (March-August, 2014). Methodology: Twenty-five genotypes obtained from the Gene bank of Kenya and farmers’ fields