The approach of using primarily satellite observations to estimate ecosystem gross primary production (GPP) without resorting to interpolation of many surface observations has recently shown promising results. Previous work has shown that the remote sensing based greenness and radiation (GR) model can give accurate GPP estimates in crops. However, the feasibility of its application and the model calibration to other ecosystems remain unknown. With the enhanced vegetation index (EVI) derived from the Moderate Resolution Imaging Spectroradiometer (MODIS) images and the surface based estimates of photosynthetically active radiation (PAR), we provide an analysis of the GR model for estimating monthly GPP using flux measurements at fifteen sites, representing a wide range of ecosystems with various canopy structures and climate characteristics. Results demonstrate that the GR model can provide better estimates of GPP than that of the temperature and greenness (TG) model for the overall data classified as non-forest (NF), deciduous forest (DF) and evergreen forest (EF) sites. Calibration of the GR model is also conducted and has shown reasonable results for all sites with a root mean square error of 47.18 g C/m 2/month. Different coefficients acquired for the three plant functional types indicate that there are shifts of importance among various factors that determine the monthly vegetation GPP. The analysis firstly shows the potential use of the GR model in estimating GPP across biomes while it also points to the needs of further considerations in future operational applications.