In Karst drainage basins, there are the ground water and underground water exchanging frequently, and the shortage of water resources due to having the special double aquifer mediums and unique surface and subsurface river systematic structure. This paper is to select 20 research sampling areas coming fromGuizhouProvince, and according to the spectral characteristics of the catchment water-holding mediums and vegetations, and using the remote sensing technique, extract the watershed vegetation index. According to the principle of principal component analysis, using the software of Spss and Matlab is to analyze the impacts of watershed vegetation type on the catchment water-holding ability, and establish the principal component analysis function. Studies have shown that: 1) the watershed vegetation coverage rate plays an important role in Karst basin water-holding ability; 2) the catchment water-holding ability is the comprehensive reflection and manifestation of the Catchment Water-storing Capacity (CWC); 3) it is much better effects and higher accuracy to monitor/forecast the catchment water-holding volume by using the vegetation indices.