To evaluate the effect of land use changes on phosphorus pools and its accumulation in the soil profile on loess soils. This study applied a modified Hedley fractionation method to investigate soil P pools in 100-cm soil profile after conversion of wheat-maize (W-M) to greenhouse vegetable (GV) or kiwi orchard (kiwi). Land use changes from W-M to GV significantly increased total P stock by 82% at 0–100-cm soil profile. In particular, conversion from W-M to GV greatly increased easily available P at 0–80 cm by 11–291%, moderately available P at 0–60 cm by 83–253%, and non-available P at 0–40 cm depth by 42–214%, respectively. Conversion from W-M to kiwi also markedly raised total P stock by 15% at 0–100-cm soil profile, in which easily available P and moderately available P at 20 cm depth increased by 161% and 130%, and non-available P at 80–100-cm depth rose by 85.6%. Land use change also caused a shift in the distribution of P pools, i.e., percentages of easily available P rose by 10–13% at 0–80 cm under GV and by 10–15% under kiwi at 0–100 cm over the W-M. Correspondingly, ratios of moderately available P also increased by 7–8% at 20–60-cm depths and by 7–10% at 0–100-cm depths. By contrast, proportions of non-available P decreased. Our results indicate a huge amount of P accumulated into the soil profile, and its solubility also increased, especially under GV, which would have significant environmental consequences. Therefore, rational P management for cash crops should arouse the attentions in all sectors of the society.