Chili plants are one of the important horticultural commodities that have many benefits. Aphids are one of the plant pests as well as a viral vector on chili plants. One control that can be applied to control it is by utilizing eco enzymes. The study used a randomized group design with seven treatments and three repeats. The treatment used is without the application of eco enzyme vegetable pesticides (A0), tobacco eco enzyme application (A1), onion eco enzyme (A2), tembelekan leaf eco enzyme (A3), red ginger eco enzyme (A4), noni fruit ecoenzyme (A5) and synthetic insecticides (A6). The observation variables consist of the intensity of the attack and the number of fruits. Observational data were analyzed using fingerprint analysis. If the treatment has a real effect, it is followed by the Smallest Real Difference test at a confidence level of 95%. Tobacco-based eco enzyme is effective in controlling aphid infestation (A. gossypii Glover) and reducing yield losses in large chili peppers (C. annuum L.).