Pesticides In Vegetables Research Articles

Unmasking the spread, carcinogenic-non carcinogenic risk characterization, and source fingerprinting of organochlorine pesticides (OCPs) in soil and vegetables of Gaya, Bihar, India.

The use of organochlorine pesticides (OCPs) in specific regions is still prevalent. Moreover, the impact of past utilization can be observed in the present environmental matrices. The present study monitored the extent of contamination of OCPs in the soil and vegetable samples of Gaya, Bihar, India. For this, 63 soil and vegetable samples were collected from the vegetable cultivated area of Gaya. The collected samples were extracted using a Soxhlet extraction unit and OCPs were analysed with a gas chromatography-mass spectrometry detector. The concentration data generated from the analysis were interpreted using statistical tools and software. Mean concentration (μg/g) of Σ19OCPs in soil from residential, agricultural, commercial, and polyhouse sites were 0.69, 2.21, 0.17, and 0.72, respectively. Similarly, in vegetable samples, mean concentration (μg/g) of Σ19OCPs were 0.91, 0.96, 1.00, and 0.67, respectively. Among the monitored vegetable types, the concentration of OCPs increased in the order: pods > tubers > leaves > fruits > roots > stem. The bioconcentration factor of 19 OCPs showed that 61.90% of vegetable samples were hyperaccumulators. The results of molecular diagnostic ratio and positive matrix factorization reported the recent inputs of heptachlor, aldrin, endrin and methoxychlor; the past application of dichlorodimethyltrichloroethane (DDT), endosulfan, and chlordane; and the degradation of DDT to its metabolites and aldrin to dieldrin, which make up an overall source profile of OCPs in study area. The study found that incremental lifetime cancer risks and hazard quotients ranged from 6.98 × 10-8 to 1.31 × 10-5 and 4.25 × 10-2 to 4.63 × 10-1, respectively in vegetable samples which indicate low to high ILCR and low non-carcinogenic risk to populations exposed to OCPs. The study indicates the long lasting impact of past pesticide use by studying the contamination in soil and vegetables, and raises serious concerns about food safety. The contamination poses direct health risk to consumers related to potential carcinogenic and endocrine disrupting effects. Thus monitoring on the ground level could be a force to modify region specific policies, health, and remediation measures related to exposure to OCPs.

A covalent organic framework-derived pretreatment for pesticides in vegetables and fruits

Sample pretreatment is an essential procedure in pesticide analysis, as the matrix effect can significantly influence the results. In this study, a covalent organic framework (COF) was synthesized using 1,2,4,5-tetrakis-(4-formylphenyl)benzene (TFPB) and benzidine (BD) to mitigate the matrix effect in vegetable and fruit samples. This COF was then used to develop a solid-phase extraction (CSPE) method. In addition, the COF was used to create a magnetic COF (MCOF) for use in magnetic solid-phase extraction (MSPE). The synthesized COF and MCOF were thoroughly characterized using scanning electron microscopy (SEM) for morphological analysis, Fourier-transform infrared spectroscopy (FT-IR) for chemical bond identification, and N2 adsorption–desorption measurements for porosity and surface area assessment. Key pretreatment parameters such as buffers, dilution rate, sorbent dosage, extraction time, elution solvent, and reuse number were optimized. The developed CSPE and MSPE showed excellent purification ability for the matrix of vegetable and fruit samples. The reuse test demonstrated that the synthesized COF and MCOF can be reused up to 15 times. Moreover, the developed CSPE and MSPE showed acceptable recoveries in spiked recovery tests, suggesting that these pretreatment methods were feasible for sample purification in pesticide analysis.

LC-MS/MS and GC-MS/MS Cross-Checking Analysis Method for 426 Pesticide Residues in Agricultural Products: A Method Validation and Measurement of Uncertainty.

A method was developed to analyze residual pesticides in various vegetables using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS). A method can analyze over 200 individual compounds, selectively separate peaks within 30 min, and meet various criteria such as those by the Codex Alimentarius Commission (CODEX). Most compounds showed recovery rates between 70 and 120%, and a relative standard deviation was within 20%. Measurement uncertainty, considering various influencing factors such as instrument precision, method sensitivity, experimental conditions, sample handling, and analytical procedures, was meticulously calculated. The expanded uncertainties of cross-checking 33 available pesticides ranged from 10.1 to 26.2 μg/kg at a confidence level of approximately 95%. Risk assessment of detected pesticides in agricultural products indicated a safety range of 0.00003-2.87240%. The developed method effectively analyzes diverse compounds simultaneously, contributing to agricultural product safety.

A novel ultrafast and highly sensitive NIR fluorescent probe for the detection of organophosphorus pesticides in foods and biological systems

The threat posed by organophosphorus pesticides (OPS) to food safety, human health, and the ecological environment is significant, which underscoring the need for the development of new detection tools. We designed and synthesized a NIR fluorescent probe PT-CES which targets carboxylesterase (CES), for the detection of OPS based on the principle of enzyme inhibition. The PT-CES is capable of instantaneous response to CES, exhibiting excellent stability, anti-interference capability. PT-CES realizes the quantitative detection of CES and OPS. It is noteworthy that PT-CES shows excellent stable and accurate detection ability in vegetable pesticide testing. It also enables the monitoring of CES activity in cells and liver tissue. This provides a novel tool for tracking the effect of OPS on CES activity in biological systems. Furthermore, it provides a useful method for ensuring food safety and enhancing pesticide residue analysis.

Impact of biochar on the degradation rates of three pesticides by vegetables and its effects on soil bacterial communities under greenhouse conditions

A 28 days pesticide degradation experiment was conducted for broccoli (Brassica oleracea L. var. italica Planch) and pakchoi (Brassica chinensis L.) with three pesticides (chlorantraniliprole (CAP), haloxyfop-etotyl (HPM), and indoxacarb (IXB)) to explore the effects of biochar on pesticide environmental fate and rhizosphere soil diversity. Rice straw biochar (RB) was applied to soil at a 25.00 t ha−1 dosage under greenhouse conditions, and its effects on the degradation of three pesticides in vegetables and in soil were investigated individually. Overall, RB application effectively facilitated CAP and HPM degradation in broccoli by 13.51–39.42% and in broccoli soil by 23.80–74.10%, respectively. RB application slowed the degradation of CAP, HPM and IXB in pakchoi by 0.00–57.17% and slowed the degradation of CAP in pakchoi by 37.32–43.40%. The results showed that the effect of RB application on pesticide degradation in crops and soil was related to biochar properties, pesticide solubility, plant growth status, and soil characteristics. Rhizosphere soil microorganisms were also investigated, and the results showed that biochar application may be valuable for altering bacterial richness and diversity. The effect of biochar application on pesticide residues in crops and soil was influenced by the vegetable variety first, and the second was pesticide characteristics. RB applied to soil at a 25.00 t ha−1 dosage under greenhouse conditions is recommended for broccoli production to ensure food safety. Our results suggested that biochar application in soil could reduce pesticide non-point source pollution, especially for highly soluble pesticides, and could affect soil microorganisms.

Pelatihan Pembuatan Pestisida Nabati Solusi Pengendalian Hama Thrips Petani Muda Desa Sukowiryo Kecamatan Jelbuk

Thrips pests are plant pest organisms (OPT) that disturb chili plants in Sukowiryo Village, Jelbuk District which can cause damage to plants, especially on the leaves. Overcoming this problem, PPK ORMAWA HIMASETA held a training on making vegetable pesticides on 22 July 2024 at the AKSATANI secretariat which was attended by 20 young farmers. The training activity aims to improve the ability of young farmers to make and use vegetable pesticides, as well as their impact on plants. The method used the counselling method regarding vegetable pesticides as an alternative effort for control of thrips pests, training in the production of vegetable pesticides, and application on chilli fields. Evaluation was conducted through pre-test and post-test questionnaires to measure knowledge and skills of young farmers. Results of the training showed that the training successful improved the knowledge and skills of young farmers in the production and use of plant-based pesticides.

Chitosan as A Plant Pathogen Control Agent: A Review

Pathogen attacks can cause decreased yields, even crop failure. Proper control can suppress pathogens' development and maintain yields. The concept of integrated pest management emphasizes the application of several techniques, one of which is the use of organic products as vegetable pesticides. Chitosan is a natural product obtained from crustacean shells or skins which can be used in various fields, including agriculture. Indonesia is one of the countries producing raw products from crustacean animals (shrimp, crab, lobster, and squid). This also has an impact on the amount of shell or skin waste produced. The processing of crustacean animal skin waste into chitosan can go through several stages, namely deproteinized, demineralization, and deacetylation. Chitosan application can be done by seed treatment, foliar spraying, soil application, and post-harvest products. The mode of action of chitosan against pathogenic fungi is binding to phospholipids in the plasma membrane of fungal cells, causing changes in hyphal morphology, degrading fungal enzymes, increasing levels of phenolics, sugar, and proline, and activating antimicrobial compounds and defense-related enzymes. The modes of action against pathogenic bacteria are interfering with gene expression, causing cell lysis, destroying bacterial biofilms, increasing defense enzyme activity, inducing systemic resistance, damaging and changing cell membranes, and causing cell wall permeability. The mode of action against pathogenic viruses is to increase the expression of genes related to defense, inhibit systemic viral multiplication and hypersensitivity response. The utilization of chitosan products is very important to study, especially in suppressing the use of chemical products and maintaining ecosystem sustainability.

Sustainable magnetic covalent organic framework materials for multi-residue analysis of pyrethroid pesticides in tobacco, fruits and vegetables

Magnetic covalent organic frameworks (MCOFs) materials were synthesized in deep eutectic solvents (DESs) instead of traditional toxic organic solvents under ambient conditions. The reaction time could be reduced to about 3 h, which was more than 12 h for the traditional synthesis method. Materials characterization indicated that the MCOFs materials with Tp and BD as monomer synthesized in ChCl-urea based DESs (i.e. Urea-MTpBD) showed good magnetic property and higher specific surface area. The Urea-MTpBD materials were used as a magnetic solid phase extraction (MSPE) sorbent for the enrichment and determination of 16 pyrethroid pesticides (PYs), coupled with GC-MS/MS technology. The effective enrichment of PYs with Urea-MTpBD materials was achieved, which was due to the synergistic effects of π-π stacking and hydrophobic interactions. The limits of detection (LODs) of 16 PYs with the developed method ranged from 2.15 to 5.67 ng/mL, while the recovery was between 70.99 % and 109.09 % with excellent intra-day and inter-day precisions (RSDintra ≤ 5.78 %, RSDinter ≤ 9.13 %). The developed method was applied successfully to determine 16 PYs in tobacco, fruits and vegetables. Furthermore, the Urea-MTpBD materials could be reused three times, which showed not only highly efficient, but also economical and sustainable.

A smartphone-assisted portable on-site detection system for organophosphorus pesticides in vegetables and fruits based on all-in-one paper-based sensors: 2,2-Dichlorovinyl dimethyl phosphate as a model

The improper use of organophosphate pesticides (OPs) can lead to residue posing a serious threat to human health and environment. Therefore, the development of a simple, portable, and sensitive detection method is crucial. Herein, a bioenzyme-nanozyme-chromogen all-in-one paper-based sensor was synthesized. Initially, the Ce/Zr-MOF with peroxidase-like activity was grown on filter paper (FP) using in-situ solvent thermal method, resulting in Ce/Zr-MOF@FP. Subsequently, the AChE-ChO-TMB system was immobilized onto Ce/Zr-MOF@FP using biocompatible gelatin, which enhanced cascade catalysis efficiency through the proximity effect. Based on the inhibition principle of OPs on AChE, we integrated this sensor with Python-based image recognition algorithm to achieve detection of OPs. Using 2,2-dichlorovinyl dimethyl phosphate (DDVP) as a model of OPs, it has good detection performance with a detection limit of 0.32 ng mL−1 and a recovery rate range of 95–107%. The potential for on-site detection of DDVP residues in vegetables and fruit samples is highly promising.

Pesticides in vegetables and fruits from Brazil and risk assessment

ABSTRACT Levels of 237 pesticides were assessed in 1063 fruit and vegetable samples from 12 São Paulo markets spanning the period May 2015 to December 2022. The QuEChERS method was employed for extraction, followed by GC-MS/MS and LC-MS/MS analysis. Findings indicated that 30% of the samples contained residues below the Maximum Residue Limits (MRLs), while 6% exceeded these. Additionally, 23% exhibited excessive residues for their respective crops and 40% had no detectable residues. Health risk evaluation focused on tomatoes, cabbage and oranges, revealing exposure within 0.002–0.9% of the Acceptable Daily Intake (ADI), indicating no chronic risks. However, pyraclostrobin in orange presented a potential acute risk for adults (112%). These results underscore the necessity for continuous monitoring of pesticide residues in fruits and vegetables to safeguard consumer health, especially considering the significant levels of consumption.

Occurrence and health risk estimate of organochlorine pesticides in fruits and vegetables matrices.

Occurrence of 20 organochlorine pesticides (OCPs) in 60 organic and non-organic fruits and vegetables matrices was undertaken using QuEChERS Method EN 15662 for sample preparation analyzed by gas chromatography-mass spectrometry (GC-MS/MS). The procedural method was validated by spiking the OCP standard solutions at three fortified levels at 10, 50, and 100 µg/kg wet weight (ww) to the real matrix of fruit and vegetable with good recovery ranging from 75 to 108% with relative standard deviation (RSD) ≤ 11%, and the limits of detection and quantification (LODs and LOQs) were 0.002-0.02μg/kg and 0.004-0.1μg/kg ww, respectively. The assessment of health risks associated with pesticide residues through consumption of vegetables and fruits and the effect of washing and peeling on concentration of various pesticides were also studied. The results showed that the concentration levels of 60% of samples were lower than the LOQs, while the rest was contaminated by OCP residues. Organic fruits and vegetables showed the absence of OCPs, while several of the studied compounds were detected from conventional agriculture. Skin removal (peeling) was the most effective strategy to eliminate or decrease pesticide residues, and should be one of the solutions to reduce the health impact of pesticides in fruits and vegetables. The analysis of health risk assessment was based on the use of the estimated average daily intake (EDI), hazard index (HI), and hazard ratio (HR) for individuals in two weight categories: children (weighing 16.7kg) and adults (weighing 60kg). The HI values were less than 1 suggesting that there was no probable non-carcinogenic health effect, except for heptachlor for children (HI of 1.285). However, the values of HR revealed that children were more susceptible to the carcinogenic health effect associated with consuming contaminated vegetables.

Pengaruh Kemasan Simpan Beras yang diberi Serbuk Daun Kemangi (Ocimum sanctum L.) Terhadap Serangan Kutu Beras (Sitophilus oryzae L.)

South Kalimantan BPS data (2022) shows that there has been a decline in rice production of 79.28 thousand tons (11.65%) so it is necessary to have rice suppliers from outside South Kalimantan to meet the rice demand target. Sitophilus oryzae is the main pest during the shelf life of rice. Controlling rice aphids can be done in various ways, including using appropriate storage media and applying vegetable pesticides to plants that are believed to be able to control them, one of which is basil. This research uses the factorial RAL method with 2 factors, namely packaging (W) and dose (D). The doses given are 0g, 10g, 14g, 18g. The packaging used is in the form of plastic containers and plastic sacks. Obtained 8 combinations, each repeated 3 times. So a total of 24 experimental units. The results of statistical analysis show that the combination of 18g of basil leaf powder in plastic packaging is the best treatment in suppressing the S. oryzae population with a mortality rate of 60%. During 30 days of observation, no new population growth was found and showed the lowest level of rice damage of 1.0% compared to other treatments.

THE EFFECT OF SOAKING DURATION OF TUBA ROOTS (Derris elliptica) ON THE MORTALITY OF THE WHITE-BACKED PLANTHOPPER PESTS (Metcalfa pruinosa)

Tuba root (Derris elliptica) is a plant that contains rotenone in roots where this content functions as a poison for insects, so tuba root has the opportunity to be used as a vegetable pesticide in pest control activities. Research on tuba roots as control of white-backed planthopper pests (Metcalfa pruinosa) on green deli water guava plants (Syzygium aqueum L.) has never been carried out, therefore research was conducted which aimed to determine the effect of long soaking of tuba roots (Derris elliptica) on the death of planthopper pests white back (Metcalfa pruinosa) on green deli water guava (Syzygium aqueum L.). The type of research used was experimental research using 5 treatments, namely soaking tuba roots for 12, 24, 36 and 48 hours. The data analysis technique uses the one way ANOVA test and the BNT test at the 0.05 level. The results of the one-way ANOVA test data analysis produced a significant value of less than 0.05, so it can be concluded that the length of soaking tuba roots influences the death rate of white-backed planthopper pests. The treatment that has the most influence on the death of white-backed planthopper pests is application three times a week using tuba root soaking for 12 hours with a mortality rate of 36%.

Investigation of variability in the matrix effect on stable isotope-labeled internal standards in liquid chromatography-tandem mass spectrometry analysis of 25 pesticides in vegetables.

The matrix effects (ME) in simultaneous analysis of pesticide residue using liquid chromatography-tandem mass spectrometry (LC-MS/MS) were evaluated by comparing the slopes of matrix-matched and reagent-only calibrations of four types of vegetable samples. Both the sampling and measurement variances of the ME were also determined using one-way analysis of variance. Substantial ion suppression (ME<-20%) was observed in komatsuna, spinach, and tomato when a modified Japanese official method was implemented. The ME magnitude varied significantly due to sample variability for some pesticides, but it varied by no more than 4% as a result of analytical procedure variance. This study also showed that the addition of stable isotope-labeled internal standards at low concentrations improved the recovery of pesticides from samples at various residue levels. The findings of this study highlight the importance and practical application of internal standards and the matrix-matched calibration method in residue analysis using LC-MS/MS.

Workers knowledge and anticipation in reducing adverse impacts of climate change

Climate change has become a global environmental issue that impacts all sectors of life, including agriculture. This study aims to determine workers’ knowledge of climate change in Karang Anyar Village Hamlet VII A Beringin Sub-district and workers’ anticipation strategies in reducing the adverse effects of climate change. This study used a qualitative approach. Informants were selected based on the criteria of wet-rice workers who have been farming for ten years by implementing semi-organic farming. Data were collected through interview techniques and literature study. Data analysis was conducted using the descriptive analysis method. The study results showed that workers know about climate change limited to seeing natural signs. Adverse impacts of climate change include a decrease in rice productivity. Workers anticipate reducing the adverse impacts of climate change by using rice varieties that are resistant to climate change, reducing the use of chemical insecticides and vegetable pesticides, and implementing a sustainable agricultural system.

Efektivitas Ekstrak Daun Kemangi (Ocimum basilicum L.) terhadap Jumlah Hama Serangga yang Menyerang Tanaman Kacang Hijau (Phaseolus radiatus L.)

Pests are a type of plant damage that can have fatal consequences for farmers, therefore pests need to be eradicated so that farmers' yields can increase, especially in green bean plants. Basil is one of the biodiversity that has the potential to be developed as a traditional medicine. This plant is a plant that has many benefits as medicine, vegetable pesticide, essential oil producer, vegetable and refreshing drink. This research aims to determine the effectiveness of basil leaf extract (Ocimu basilicum L.) on the number of insect pests that attack green bean plants (Phaseolus radiatus L.). The parameters observed were the number of insect pests before and after treatment. The object of this research was green bean plants, the number of treatments in this research was 4 treatments (0% basil leaf extract, 10% basil leaf extract, 20% basil leaf extract, and 30% basil leaf extract) and 6 replications. Research data is descriptive data. From the research data, it was found that there was a decrease in the number of insect pests before and after treatment in the K3 treatment, the number of insect pests before treatment was 12 and after treatment there were 11 insect pests. In treatment K1-K2 there was no decrease in the number of insect pests before or after treatment. In treatment K1, the number of insect pests before treatment was 9 and after treatment there were 14 insect pests. In the K2 treatment, the number of insect pests before treatment was 12 and after treatment there were 19 insect pests. The results of the study concluded that administering basil leaf extract (Ocimum basilicum L.) had no significant effect on the number of insect pests attacking green bean plants.

The effectiveness Assay of Vegetable Pesticides Papaya and Mahogany Leaf Extracts in Pest Control of Spodoptera exigua (Lepidoptera: Noctuidae) on Onion (Allium ascalonicum L) Cultivation

Spodoptera exigua is the main pest that attacks the onion crop. S.exigua attack can cause crop failure reaching 57%-100%. The use of natural ingredients from plants for vegetable pesticides such as papaya leaves and mahogany leaves containing secondary metabolite compounds: alkaloids, papain, saponins, tannins, terpenoids, flavonoids and steroids that can damage the feeding activity system and damage the pest growth system which eventually the pest will die. This study uses a non-factorial RAL (Completely Randomized Rack) method with 7 treatments M0= Control, M1= Papaya leaf extract 30%, M2= Papaya leaf extract 60%, M3= Mahogany leaf extract 30%, M4= Mahogany leaf extract 60%, M5 = Papaya leaf extract 15% + mahogany leaf extract 15%, M6= Papaya leaf extract 30% + mahogany leaf extract 30%. The highest mortality result is 72.50% with the treatment of 60% papaya extract vegetable pesticide and the lowest is 27.50% with the treatment of 30% mahogany extract vegetable pesticide. The initial symptoms of death of S. exigua caterpillars are characterized by larvae that are less active in moving, moving to the surface of the container, moving away from the feed, convulsing and finally dying. The mortality of S. exigua larvae at 24 JSA killed a total of 14 larvae and increased with each subsequent treatment. The results of the vegetable pesticide test on natural enemies using extracts that produce the highest percentage of mortality on S. exigua is 100%. This indicates that the extract is safe for the survival of natural enemies. Plant-based pesticides papaya leaf extract, mahogany leaves and a combination of papaya and mahogany leaves have an effect on the mortality of S. exigua caterpillars

2D magnetic nanoporous carbon with bridge-type aptamer assembly for pesticide detection based on a regenerative field-effect transistor biosensor

Field-effect transistor (FET)-based biosensors have garnered attention due to their sensitivity to surface charge changes and advantages in real-time detection. However, these biosensors often suffer from several disadvantages such as their limited sensitivity and a high cost. Here, we reported an ultra-sensitive and reusable nanoporous-based FET biosensor hybridized with bridge-type aptamers. The sensor consisted of a two-dimensional magnetic iron-cobalt nanoparticle-embedded nanoporous carbon (Fe-Co/NPC) functionalized with bridge-type aptamers. The edges of the nanopores exhibited high surface energy and chemical activity, enabling effective interactions with the aptamers. Moreover, the nanoporous structures provided additional space, which could improve molecular recognition sites and sensor sensitivity. Our sensor could be used to detect organophosphorus pesticides (OPs) in vegetables down to 100 fM in real time and identify OPs from other pesticides. Furthermore, the magnetic properties of Fe-Co/NPC allowed us to reuse a single sensor chip up to 8 times, which significantly reduced the production cost of our sensors. In this respect, our Fe-Co/NPC-FET sensor could be a powerful tool for practical applications such as food safety detection.

Socialization and Training on Making Vegetable Pesticides from Papaya Leaves (Carica Papaya. L) and Garlic (Allium Sativum) with the Community of Taba Air Pauh Village

The purpose of this community service project is to transfer information and skills about the art of producing aromatherapy candles using natural ingredients like coffee, lemongrass, and lime to the residents of Tapak Gedung Village, Tebat Karai District, Kepahiang Regency. The technique used for community service projects in Tapak Gedung Village, Tebat Karai District, Kepahiang Regency, including teaching people how to make aromatherapy candles out of lime and coffee beans. The residents of Tapak Gedung Village, Tebat Karai District, Kepahiang Regency, have learned how to make aromatherapy candles from natural materials nearby, and this could lead to the residents of Tapak Gedung Village becoming financially independent. This is based on the outcomes of community service activities involving training in the production of aromatherapy candles from coffee beans and lime.

Pesticide Residue and Bio-pesticides in Vegetable Crops

Intensive vegetable production using pesticides has biggest threat to growers and the consumers. In such instances the accumulation of pesticide residues is increased due to relatively short pre-harvest interval. Use of pesticides without knowing the label claim information increases the cost of production, increases the number of spray and labour cost, ultimately leading to decrease in farmers profitability. Hence, the adoption of pesticide as per label claim is very much essential. The level of residues should be below the maximum residue limit (MRL) at the time of harvest. Most of the detected pesticides in vegetables are not registered by Central Insecticide Board and Registration committee (CIBRC) for use on that specific vegetable which is the off label use of pesticides. Crops grouping is the development of a model that allows extrapolation of residue data from a few representative crops to many other crops in the same group. This allows establishment of residue tolerances for the entire group of crops based on the residue values from certain key crops that are similar. The acceptance of representative crop is a critical component of the savings from using the crop groups. IR-4’s involvement with efforts to remove pesticide residues as a barrier for exports for US-grown specialty crops has been growing in importance over the last 20 years. By establishing a common MRL on a specialty crop from a particular crop protection product use, trade irritants between the two countries can be prevented before they have the potential to become a major problem for specialty crop growers on each side of the border. The U.S./Canadian specialty crop partnership has yielded valuable results for all the stakeholders involved. IR4 signed MOUs with Canada, New Zealand, Brazil, Costa Rica, and Colombia. This model is also much needed for India to regulate the pesticide label claims for numerous crops.