Vector-transmitted Diseases Research Articles

Gambaran Kepadatan Dan Identifikasi Lalat (Diptera) Di Pasar Waiheru Perumnas Kecamatan Baguala Kota Ambon

Flies are vectors for disease transmission, especially mechanically and through their vomit and feces. Items, especially food, are usually contaminated by human feces, rubbish, the saliva of sick people, scars from animal carcasses and other things that are covered in flies. Flies can throw feces on food so that the food becomes contaminated with fly eggs and larvae. Disruption of comfort, eyesore, itching of the skin, causing discomfort, decreased appetite. To determine the density and identification of flies in the Waiheru Perumnas market, Baguala District, Ambon City. Type of descriptive research that describes a condition based on observation and examination of the Ambon Environmental Health Engineering Center (BTKL) Laboratory. The aim of descriptive research is to describe the density and identification of flies in the Waiheru Perumnas market, Baguala District, Ambon City. Measuring the density of flies in the Waiheru Perumnas market, Baguala District, Ambon City is based on 3 points, 10 measurements at each point in the morning, at the first point (los fish) amounted to 87 flies, at point II (vegetable stall there were 55 flies and at point III (fruit stall) there were 45 flies. Identification of fly types resulted in 3 types of flies identified, namely Musca Domestica (house fly), Cliphora Sp (green flies) and Sarcophaga (meat flies). The density of flies in the Waiheru Perumnas market, Baguala District, Ambon City, based on the installation of fly grills based on the 10 highest points, was 187 flies and at the 5 highest points was 134 flies with 23 flies caught in the fly trap. From the results obtained, it can be seen that the density of flies is very high so it is necessary to deal with fly breeding sites and fly control measures.

COVID-19 in Scottish care homes: A metapopulation model of spread among residents and staff

The movement of populations between locations and activities can result in complex transmission dynamics, posing significant challenges in controlling infectious diseases like COVID-19. Notably, networks of care homes create an ecosystem where staff and visitor movement acts as a vector for disease transmission, contributing to the heightened risk for their vulnerable communities. Care homes in the UK were disproportionately affected by the first wave of the COVID-19 pandemic, accounting for almost half of COVID-19 deaths during the period of 6th March – 15th June 2020 and so there is a pressing need to explore modelling approaches suitable for such systems. We develop a generic compartmental Susceptible - Exposed - Infectious - Recovered - Dead (SEIRD) metapopulation model, with care home residents, care home workers, and the general population modelled as subpopulations, interacting on a network describing their mixing habits. We illustrate the model application by analysing the spread of COVID-19 over the first wave of the COVID-19 pandemic in the NHS Lothian health board, Scotland. We explicitly model the outbreak’s reproduction rate and care home visitation level over time for each subpopulation and execute a data fit and sensitivity analysis, focusing on parameters responsible for inter-subpopulation mixing: staff-sharing, staff shift patterns and visitation. The results from our sensitivity analysis show that restricting staff sharing between homes and staff interaction with the general public would significantly mitigate the disease burden. Our findings indicate that protecting care home staff from disease, coupled with reductions in staff-sharing across care homes and expedient cancellations of visitations, can significantly reduce the size of outbreaks in care home settings.

Multiple endemic equilibria in an environmentally-transmitted disease with three disease stages

We construct, analyze and interpret a mathematical model for an environmental transmitted disease characterized for the existence of three disease stages: acute, severe and asymptomatic. Besides, we consider that severe and asymptomatic cases may present relapse between them. Transmission dynamics driven by the contact rates only occurs when a parameter R∗>1, as normally occur in directly-transmitted or vector-transmitted diseases, but it will not adequately correspond to a basic reproductive number as it depends on environmental parameters. In this case, the forward transcritical bifurcation that exists for R∗<1, becomes a backward bifurcation, producing multiple steady-states, a hysteresis effect and dependence on initial conditions. A threshold parameter for an epidemic outbreak, independent of R∗ is only the ratio of the external contamination inflow shedding rate to the environmental clearance rate. R∗ describes the strength of the transmission to infectious classes other than the I-(acute) type infections. The epidemic outbreak conditions and the structure of R∗ appearing in this model are both responsible for the existence of endemic states.

Integrating host biological and ecological variables to predict probability of haemosporidian infection in raptors

Variations in host traits that influence their exposure and susceptibility may impact probability of vector-transmitted diseases. Therefore, identifying the predictors of infection probability is necessary to understand the risk of disease outbreaks during expanding environmental perturbation. Here, we conducted a large survey based on microscopic examination and molecular analysis of haemosporidian parasite infection in raptors rescued at the Beijing Raptor Rescue Centre. Combining these data with biological and ecological variables of the raptors, we determined predictors that affect the probability of haemosporidian infection using generalized linear mixed models and multimodel inference. Our results showed that infection probability exhibited considerable variation across host species in raptors, and body mass, sex, and evolutionary history played relatively weaker roles in driving infection probability. Instead, activity pattern, age, geographic range size, migration distance, and nest type were important predictors of the probability of haemosporidian infection, and the role of each predictor differed in the three main haemosporidian genera (Plasmodium, Haemoproteus, and Leucocytozoon). This macro-ecological analysis will add to our understanding of host traits that influence the probability of avian haemosporidian infection and will help inform risk of emerging diseases.

Biodiversity of mosquitoes (Diptera: Culicidae) in phytotelmata from Car Nicobar Island, India.

The Culicidae mosquito family breeds in various natural and artificial water bodies. Phytotelmata are plant structures that hold water and are used by many mosquito species during their life cycle. Mosquitoes are disease vectors, and phytotelmata play an important role in harbouring them. Investigating these phytotelmata will facilitate to develop appropriate site specific vector control strategy. Approximately 750 phytotelmata in 16 locations on Car Nicobar Island was examined. Mosquito larvae were collected from different habitats such as tree holes, root holes, leaf axils, and bamboo stumps. On average, the number of larvae per 10 ml of water ranged from 0.003 to 3.833. Among the 16 mosquito species identified, there were vectors of malaria (Anopheles sundaicus), dengue (Aedes aegypti and Aedes albopictus) and filariasis (Culex quinquefasciatus), belonging to seven genera. The most common phytotelmata were coconut trees (17.5%) followed by Java apple trees (10.3%) and bamboo stumps (7.1%). The most widespread mosquito species was Aedes edwardsi, followed by Aedes malayensis and Ae. albopictus, and the per cent of phytotelmata that contained these species were 42.6, 22.0, and 16.8% respectively. Anopheles mosquitoes had the highest container index (6.1%) in root holes, while Aedes and Culex mosquitoes were more frequently found in bamboo stumps. The alpha biodiversity indices indicated that the mosquito population in Car Nicobar Island was highly diverse (S = 16; DMg = 36.9). Tree holes showed the highest diversity, as all 16 species were collected from them. Assessment of phytotelmata, there is a significant relationship observed between mosquito genus, village, habitat, and tree families, on the remote island of Car Nicobar. Considering the presence of disease-transmitting mosquito vectors in the phytotelmata, these habitats should be considered when implementing control measures to eliminate mosquito-borne infections.

Decoding the genome of bloodsucking midge Forcipomyia taiwana (diptera: Ceratopogonidae): Insights into odorant receptor expansion

Biting midges, notably those within the Ceratopogonidae family, have long been recognized for their epidemiological significance, both as nuisances and vectors for disease transmission in vertebrates. Despite their impact, genomic insights into these insects, particularly beyond the Culicoides genus, remain limited. In this study, we assembled the Forcipomyia taiwana (Shiraki) genome, comprising 113 scaffolds covering 130.4 Mbps—with the longest scaffold reaching 7.6 Mbps and an N50 value of 2.6 Mbps—marking a pivotal advancement in understanding the genetic architecture of ceratopogonid biting midges. Phylogenomic analyses reveal a shared ancestry between F. taiwana and Culicoides sonorensis Wirth & Jones, dating back approximately 124 million years, and highlight a dynamic history of gene family expansions and contractions within the Ceratopogonidae family. Notably, a substantial expansion of the odorant receptor (OR) gene family was observed, which is crucial for the chemosensory capabilities that govern biting midges' interactions with their environment, including host seeking and oviposition behaviors. The distribution of OR genes across the F. taiwana genome displays notable clusters on scaffolds, indicating localized tandem gene duplication events. Additionally, several collinear regions were identified, hinting at segmental duplications, inversions, and translocations, contributing to the olfactory system's evolutionary complexity. Among the 156 ORs identified in F. taiwana, 134 are biting midge-specific ORs, distributed across three distinct clades, each exhibiting unique motif features that distinguish them from the others. Through weighted gene co-expression network analysis, we correlated distinct gene modules with sex and reproductive status, laying the groundwork for future investigations into the interplay between gene expression and adaptive behaviors in F. taiwana. In conclusion, our study not only highlights the unique olfactory repertoire of ceratopogonid biting midges but also sets the stage for future studies into the genetic underpinnings of their unique biological traits and ecological strategies.

FINAL SIZE RELATIONS FOR SOME COMPARTMENTAL MODELS IN EPIDEMIOLOGY

The summary measurement of an epidemic such as the final size is an important quantity that allows us to approximate the impact of the disease on the affected region. In recent years, final size measurements for vector-transmitted diseases have acquired importance because of the increased concern for mosquito-borne diseases like dengue, Zika, Chikungunya, etc. However, analytical expressions for this estimate in the stage-structured models applicable to vector-borne diseases are less, mostly focused on the classical Kermack–McKendrick model. In this paper, we first calculate the final size expressions for an SIR model with carrier state and a SEIR–SI host–vector model. Then we extend this for the SEIR–SI host–vector model with treatment class in the host, as well as for the model with vertical transmission in the vector population. Finally, we verify our final size expression with some real scenarios.

A small-molecule approach to restore female sterility phenotype targeted by a homing suppression gene drive in the fruit pest Drosophila suzukii.

CRISPR-based gene drives offer promising prospects for controlling disease-transmitting vectors and agricultural pests. A significant challenge for successful suppression-type drive is the rapid evolution of resistance alleles. One approach to mitigate the development of resistance involves targeting functionally constrained regions using multiple gRNAs. In this study, we constructed a 3-gRNA homing gene drive system targeting the recessive female fertility gene Tyrosine decarboxylase 2 (Tdc2) in Drosophila suzukii, a notorious fruit pest. Our investigation revealed only a low level of homing in the germline, but feeding octopamine restored the egg-laying defects in Tdc2 mutant females, allowing easier line maintenance than for other suppression drive targets. We tested the effectiveness of a similar system in Drosophila melanogaster and constructed additional split drive systems by introducing promoter-Cas9 transgenes to improve homing efficiency. Our findings show that genetic polymorphisms in wild populations may limit the spread of gene drive alleles, and the position effect profoundly influences Cas9 activity. Furthermore, this study highlights the potential of conditionally rescuing the female infertility caused by the gene drive, offering a valuable tool for the industrial-scale production of gene drive transgenic insects.

Serologic Survey of Selected Arthropod-Borne Pathogens in Free-Ranging Snowshoe Hares (Lepus americanus) Captured in Northern Michigan, USA.

Snowshoe hares (Lepus americanus) in the Upper Peninsula (UP) of Michigan, USA, occupy the southern periphery of the species' range and are vulnerable to climate change. In the eastern UP, hares are isolated by the Great Lakes, potentially exacerbating exposure to climate-change-induced habitat alterations. Climate change is also measurably affecting distribution and prevalence of vector-borne pathogens in North America, and increases in disease occurrence and prevalence can be one signal of climate-stressed wildlife populations. We conducted a serosurvey for vector-borne pathogens in snowshoe hares that were captured in the Hiawatha National Forest in the eastern UP of Michigan, USA, 2016-2017. The most commonly detected antibody response was to the mosquito-borne California serogroup snowshoe hare virus (SSHV). Overall, 24 (51%) hares screened positive for SSHV antibodies and of these, 23 (96%) were confirmed positive by plaque reduction neutralization test. We found a positive association between seroprevalence of SSHV and live weight of snowshoe hares. Additionally, we detected a significant effect of ecological land type group on seroprevalence of SSHV, with strong positive support for a group representing areas that tend to support high numbers of hares (i.e., acidic mineral containing soils with cedar, mixed swamp conifers, tamarack and balsam fir as common overstory vegetation). We also detected and confirmed antibodies for Jamestown Canyon virus and Silverwater virus in a single hare each. We did not detect antibodies to other zoonotic vector-borne pathogens, including Lacrosse encephalitis virus, West Nile virus, Borrelia burgdorferi, Powassan virus, and Francisella tularensis. These results provide a baseline for future serological studies of vector-transmitted diseases that may increase climate vulnerability of snowshoe hares in the UP of Michigan, as well as pose a climate-related zoonotic risk.

CURRENT STATUS OF METATRANSCRIPTOMIC AND RELATED STUDIES IN HEMATOPHAGOUS DISEASE-TRANSMITTING VECTORS

The incidence of numerous vector-borne diseases (VBDs) has recently increased alarmingly due to various widespread factors, including unplanned urbanization, greater human mobility, environmental changes, vector resistance to insecticides, and evolving pathogens. In this context, the World Health Organization (WHO) has repositioned effective and sustainable vector control as a key approach to prevent and eliminate VBDs. It has been shown that the microbiome influences development, nutrition, and pathogen defense in disease-transmitting vectors such as mosquitoes, sandflies, tsetse flies, triatomine bugs, and ticks. Consequently, understanding the endogenous regulation of vector biology can aid in developing effective approaches for vector control. In this respect, a metatranscriptomic approach analyzes all the expressed RNAs in an environmental sample (meta-RNAs) and can thus reveal how the metabolic activities of the microbiome influence vector biology. This review includes an extensive analysis of available literature on microbial and viral studies for some of the major hematophagous disease-transmitting arthropods, with a focus on studies that used next generation sequencing (NGS) approaches. Since a consensus terminology for these “meta-sequencing analyses” has not yet been established, a definition of these terms is presented here to provide the framework for systematically sorting the available information for each of the VBDs analyzed here to single out metatranscriptomic analyses. Finally, key gaps in knowledge were identified for some of these hematophagous disease-transmitting arthropods which will prove very useful for driving future studies.

Enhanced vegetable production in hydroponic systems using decontamination of closed circulating fluid

While plant microorganisms can promote plants by producing natural antibiotics, they can also be vectors for disease transmission. Contamination from plant management practices and the surrounding environment can adversely affect plants, leading to infections and hindered growth due to microbial competition for nutrients. The recirculation of nutrient-rich fluids can facilitate the transport of microorganisms between vegetables in the hydroponic production system. This issue can be addressed through the application of the decontamination method in the hydroponic liquid. Ultraviolet light (UV-C) has been employed for microbiology, and its effects on lettuce were evaluated in this study. This study aims to assess the effectiveness of a decontamination system using UV-C in hydroponic solutions during nutrient recirculation in hydroponics. We evaluated the time required for lettuce plants to reach their maximum height, as well as their pigment content, phenolic compounds, antioxidant capacity, and micro and macronutrient levels. The evaluation was conducted under two photoperiods (18 and 20 hours) in lettuce samples exposed to UV-C in the hydroponic fluid, with control groups not exposed to UV-C. The application of the UV-C decontamination system in hydroponic circulation water containing nutrients accelerated plant growth while maintaining nutritional values equal to or higher than those in the control groups without such a system. The results of microorganism control highlight the potential application of this technique for enhancing and expediting vegetable production. This approach reduces production time and enhances nutrient absorption and the content of certain compounds and minerals.

Lysinibacillus fusiformis: A novel mosquitocidal bacterium isolated from Western Ghats, Kerala, India

An extensive field survey was carried out from December 2021 to January 2022 for the collection of soil samples in the Western Ghat region of the Wayanad district of Kerala, India, to isolate potent novel mosquitocidal bacteria which could be used as efficient formulations to control mosquito vectors. Several bacterial colonies were isolated and screened, for mosquitocidal activity. Toxicity assay showed that only one bacterium isolated from forest loamy soil had promising larvicidal activity against Culex quinquefasciatus the lymphatic filariasis vector (LC50: 0.03 mg L-1 &amp; LC90: 0.07 mg L-1) and moderate activity was shown against the dengue vector, Aedes aegypti (LC50: 1.03 mg L-1 &amp; LC90: 1.8 mg L-1). The bacterium was identified as Lysnibacillus fusiformis by a phylogenetic tree constructed using 16S rRNA genome sequencing. This is the first report that L. fusiformis isolated from the forest loamy soil of the Western Ghats, Kerala, which showed proficient mosquitocidal activity against disease-transmitting mosquito vectors.

EKOLITERASI SISWA MELALUI PENGELOLAAN SAMPAH DAN PELATIHAN PEMBUATAN ECOBRICK

Indonesia has main problems, namely basic education, the economy, and the environment based on the Global Competitive Index, which ranks 45 out of 140. The low level of eco-literacy is a serious problem that is of concern to the world for Indonesia. This is proven by Indonesia's crowning as the second country that contributes the most waste, especially plastic waste. Based on a national scope (assuming a population of 180 million people, the waste production rate for each person is two liters per day with a composition of 6.17%), the amount of waste accumulated in Indonesia annually reaches 1,599,000 tons. SDN 211/IV Jambi City already has rubbish bins that are differentiated based on the type of waste, namely organic and inorganic, however, the rubbish thrown into the rubbish bin is not grouped by type. Likewise, waste processing has not been managed properly, which has the potential to become a nest for disease-transmitting vectors due to the accumulation of waste behind schools. Ecoliteracy and one way of processing plastic waste is by eco-bricks. The solution offered is socialization and counseling about eco-literacy or increasing pro-environmental behavior as a basis that must be understood from an early age, especially the waste reduction paradigm, and providing technical guidance for making eco-bricks. The results were that students at SDN 211/IV Jambi City understood the importance of managing waste into eco-bricks and how to make them. Hopefully, this activity will continue in the future, and students can become cadres initiating plastic waste management in elementary schools in Jambi City.

Comparisons of aerosol generation across different musical instruments and loudness

Respiratory aerosols can serve as vectors for disease transmission, and aerosol emission is highly activity-dependent. COVID-19 severely impacted the performing arts due to concerns about disease spread by respiratory aerosols and droplets generated during singing and playing musical instruments. Aerosol generation from woodwind and brass performance is less understood compared to singing due to uncertainty about how the diverse range of musical instruments may impact respiratory aerosol concentrations and size distributions. Here, aerosol number and mass concentrations along with size distributions were measured for breathing, speaking, and playing four different woodwind and brass instruments by 23 professional instrumentalists. We find that a 1 dBA increase in sound pressure level corresponds to a ∼10% increase in aerosol number concentration. The aerosol size distribution is consistent with that of breathing. Differences in aerosol emission across musical instruments can be partly explained by the loudness of performance. Measuring aerosol generation from single notes or simple songs may be sufficient to characterise the aerosol emission range during actual performance, provided a range of loudnesses are accessed. These results provide insight into the factors contributing to aerosol emission during musical performance and facilitate risk assessments associated with infectious respiratory disease transmission in the performing arts.

Multi-sensorial perceptions of risk: the aesthetics behind (muco)cutaneous leishmaniasis-related stigma in Ecuador

Previous research on the stigma associated with cutaneous leishmaniasis, a vector-transmitted parasitic disease, focuses on aesthetic appearance affectation as the leading cause of stigmatisation. However, Indigenous populations in the hinterland of Amazonian Ecuador trigger stigma expressions by recognising (muco)cutaneous leishmaniasis, primarily through atypical smell, followed by the odd voice sound, appearance and taste. This empirical way of recognising symptoms relies on embodied forms of identifying a disease, contrasting the Western supremacy of visuality and demanding to be understood via multi-sensorial anthropology. Through ethnographic research and data retrieved from eighty-three semistructured interviews and fifteen focus groups in seven Ecuadorian ethnic groups – including six Indigenous groups in the Amazon region – this paper analyses how the sensorium is a health thermometer. Findings reveal that differentiated cultural responses to a sense of peril, contagion and social (self)rejection, understood as stigma expressions, are linked to the holistic approach to health (or well-being) shared by Indigenous populations. In forest societies, well-being is explained through successful (non-)human relationships, and disease permeates through bodies that lack balanced relations.

BIOINSECTICIDE POTENTIAL OF ETHANOL EXTRACTS FROM &lt;i&gt;Persea americana&lt;/i&gt; (LAURACEAE) SEEDS ON &lt;i&gt;Aedes aegypti&lt;/i&gt; MOSQUITOES

Mosquitoes represent the most important agent disseminating infectious diseases like yellow fever, dengue, chikungunya, and malaria, among others. An essential strategy for its control is killing them at immature stages using industrial insecticides. However, those chemicals often generate resistance and affect the environment and human health. Agricultural and plant by-products constitute a new sustainable option to obtain harmless and eco-friendly bioinsecticides to prevent mosquitoes from spreading. This study aimed to investigate the phytochemical profile of Persea americana Mill (Lauraceae) seed extracts and their insecticide activity against Aedes aegypti at larval and pupal stages. The ethanol extracts from avocado seeds were obtained by Maceration/stirring (MaE) and Soxhlet extraction (SE) methods. The main chemical profile was determined by quantitative and UPLC assays. Insecticide activity was assessed by the exposition of mosquitoes at larval and pupal stages to seed extracts. Human cell lines were used to evaluate the cytotoxicity. Soxhlet methodology was more efficient in the extraction of P. americana seeds metabolites (42.13±1.76 mg/mL) compared with MaE (20.46±1.66 mg/mL) (p&lt; 0.05). Additionally, SE showed a higher amount of polyphenols (5.12±0.18 mg/mL). The UPLC spectra analysis revealed the presence of polyphenols, mainly catechin, and neolignan constituents. Both extracts showed larvicidal and pupicidal effects, but SE was more active at lower concentrations. Moreover, no significant toxic effects on human monocytes and fibroblast cell lines were found after treatment. In sum, avocado seed by-products can be considered an eco-friendly insecticide and its use may help to substantially decrease the vector-transmitted diseases in developing countries.

Microbiota in disease-transmitting vectors.

Haematophagous arthropods, including mosquitoes, ticks, flies, triatomine bugs and lice (here referred to as vectors), are involved in the transmission of various pathogens to mammals on whom they blood feed. The diseases caused by these pathogens, collectively known as vector-borne diseases (VBDs), threaten the health of humans and animals. Although the vector arthropods differ in life histories, feeding behaviour as well as reproductive strategies, they all harbour symbiotic microorganisms, known as microbiota, on which they depend for completing essential aspects of their biology, such as development and reproduction. In this Review, we summarize the shared and unique key features of the symbiotic associations that have been characterized in the major vector taxa. We discuss the crosstalks between microbiota and their arthropod hosts that influence vector metabolism and immune responses relevant for pathogen transmission success, known as vector competence. Finally, we highlight how current knowledge on symbiotic associations is being explored to develop non-chemical-based alternative control methods that aim to reduce vector populations, or reduce vector competence. We conclude by highlighting the remaining knowledge gaps that stand to advance basic and translational aspects of vector-microbiota interactions.

MicroRNAs reshape the immunity of insects in response to bacterial infection.

The interaction between bacteria and insects can significantly impact a wide range of different areas because bacteria and insects are widely distributed around the globe. The bacterial-insect interactions have the potential to directly affect human health since insects are vectors for disease transmission, and their interactions can also have economic consequences. In addition, they have been linked to high mortality rates in economically important insects, resulting in substantial economic losses. MicroRNAs (miRNAs) are types of non-coding RNAs involved in regulating gene expression post-transcriptionally. The length of miRNAs ranges from 19 to 22 nucleotides. MiRNAs, in addition to their ability to exhibit dynamic expression patterns, have a diverse range of targets. This enables them to govern various physiological activities in insects, like innate immune responses. Increasing evidence suggests that miRNAs have a crucial biological role in bacterial infection by influencing immune responses and other mechanisms for resistance. This review focuses on some of the most recent and exciting discoveries made in recent years, including the correlation between the dysregulation of miRNA expression in the context of bacterial infection and the progression of the infection. Furthermore, it describes how they profoundly impact the immune responses of the host by targeting the Toll, IMD, and JNK signaling pathways. It also emphasizes the biological function of miRNAs in regulating immune responses in insects. Finally, it also discusses current knowledge gaps about the function of miRNAs in insect immunity, in addition to areas that require more research in the future.

Diagnosis of microfilaria using modified Knott’s technique in buffalo and its treatment

Filariasis is a vector-transmitted parasitic disease caused by Filarioidea nematode with world wide spread especially in tropical and sub-tropical countries. This is highly prevalent in hot and humid areas of India mainly hilly parts of India like Tarai region of Uttar Pradesh and coastal areas of Andhra Pradesh. The tentative diagnosis was carried out by direct faecal smear examinations, wet blood film observation and blood smear examinations, and modified Knott’s technique, further case history was reviewed. The collected blood was subjected to different staining techniques including modified Knott’s technique. On modified Knott’s method examination detected microfilaria is long slender anterior blunt and tapering posterior ends. Ivermectin 200 μg/kg body weight subcutaneous route (orally microfilaricide 50 ug/ kg) is used as a curative drug in present clinical case and animal was responded well with the treatment. Hematological parameters of buffalo includes decreased hemoglobin (6.5 grs /dl), packed cell volume (20%), and total erythrocytic count (4.5×106/ul) were recovered within 1 week after treatment.

Improved Ecosystem Services and Environmental Gentrification after Rehabilitating Brazilian Urban Streams

The high levels of environmental pollution observed in urban freshwater ecosystems result in losses of ecosystem goods and services, reducing the well-being of human populations in densely populated tropical cities. The Belo Horizonte Metropolitan Region (BHMR) resembles other megacities in the Global South, with inadequate collection and treatment of domestic sewage being an important source of environmental degradation. However, urban stream rehabilitation can improve ecosystem quality and the physical and mental well-being of local citizens. Therefore, the objective of this study was to assess whether the rehabilitation of three BHMR streams and the increased provision of ecosystem goods and services for local residents were associated with environmental gentrification and public health issues. To achieve this objective, we asked three questions. (i) Was there socioeconomic improvement in the households located near the rehabilitated streams? (ii) Did property values appreciate near the rehabilitated streams? (iii) Was the relative incidence of diseases decreased in the residents living near the rehabilitated streams? We tested three hypotheses. (H1) The socioeconomic profile of the households in the areas neighboring the rehabilitated streams improved. (H2) The property values of residences in the areas neighboring the rehabilitated streams increased. (H3) The incidence of waterborne and other types of diseases in the areas neighboring the rehabilitated streams decreased. To answer the first question and hypothesis, we compared 2000 to 2010 census tracts in the areas neighboring the rehabilitated streams with others in the municipality of Belo Horizonte (BH). We observed non-significant socioeconomic and demographic differences. To answer the second question and hypothesis, we used real estate transactions between 2009 and 2018 using hedonic models. After controlling for multiple interacting variables, we observed real estate appreciation after stream rehabilitation. To answer the third question and hypothesis, we analyzed the association between the prevalence of waterborne diseases, vector-borne diseases, and other diseases in the residents living near the rehabilitated streams. To do so, we analyzed hospitalization data from 2005 to 2016 for residents neighboring the streams versus BH. We observed a significant increase in the prevalence of waterborne and vector-transmitted diseases until two years after the stream rehabilitation, followed by a decrease until 2010. Trends for other types of diseases differed from these and mostly followed the general tendencies of BH, indicating different temporal variations. Our results highlight that ecosystem goods and services fostered by urban stream rehabilitation have potential contributions to the well-being of urban populations. The economic analyses applied in the paper have clear policy implications in support of urban stream rehabilitation. Although South American countries lack mandatory rehabilitation investments, Belo Horizonte’s example can inspire other interventions in tropical megacities.