Vector Integration Research Articles

InCliniGene enables high-throughput andcomprehensive in vivo clonal tracking toward clinical genomics data integration.

High-throughput clonal tracking in patients under hematopoietic stem cell gene therapy with integrating vector is instrumental in assessing bio-safety and efficacy. Monitoring the fate of millions of transplanted clones and their progeny across differentiation and proliferation over time leverages the identification of the vector integration sites, used as surrogates of clonal identity. Although γ-tracking retroviral insertion sites (γ-TRIS) is the state-of-the-art algorithm for clonal identification, the computational drawbacks in the tracking algorithm, based on a combinatorial all-versus-all strategy, limit its use in clinical studies with several thousands of samples per patient. We developed the first clonal tracking graph database, InCliniGene (https://github.com/calabrialab/InCliniGene), that imports the output files of γ-TRIS and generates the graph of clones (nodes) connected by arches if two nodes share common genomic features as defined by the γ-TRIS rules. Embedding both clonal data and their connections in the graph, InCliniGene can track all clones longitudinally over samples through data queries that fully explore the graph. This approach resulted in being highly accurate and scalable. We validated InCliniGene using an in vitro dataset, specifically designed to mimic clinical cases, and tested the accuracy and precision. InCliniGene allows extensive use of γ-TRIS in large gene therapy clinical applications and naturally realizes the full data integration of molecular and genomics data, clinical and treatment measurements and genomic annotations. Further extensions of InCliniGene with data federation and with application programming interface will support data mining toward precision, personalized and predictive medicine in gene therapy. Database URL: https://github.com/calabrialab/InCliniGene.

A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes.

For decades, Agrobacterium rhizogenes (now Rhizobium rhizogenes), the causative agent of hairy root disease, has been harnessed as an interkingdom DNA delivery tool for generating transgenic hairy roots on a wide variety of plants. One of the strategies involves the construction of transconjugant R. rhizogenes by transferring gene(s) of interest into previously constructed R. rhizogenes pBR322 acceptor strains; little has been done, however, to improve upon this system since its implementation. We developed a simplified method utilising bi-parental mating in conjunction with effective counterselection for generating R. rhizogenes transconjugants. Central to this was the construction of a new Modular Cloning (MoClo) compatible pBR322-derived integration vector (pIV101). Although this protocol remains limited to pBR322 acceptor strains, pIV101 facilitated an efficient construction of recombinant vectors, effective screening of transconjugants, and RP4-based mobilisation compatibility that enabled simplified conjugal transfer. Transconjugants from this system were tested on Lotus japonicus and found to be efficient for the transformation of transgenic hairy roots and supported infection of nodules by a rhizobia symbiont. The expedited protocol detailed herein substantially decreased both the time and labour for creating transconjugant R. rhizogenes for the subsequent transgenic hairy root transformation of Lotus, and it could readily be applied for the transformation of other plants.

Chlamydia suis displays high transformation capacity with complete cloning vector integration into the chromosomal rrn-nqrF plasticity zone.

Chlamydia, comprising several human and zoonotic pathogens, is a genus of the conserved bacterial phylum Chlamydiota. Their obligate intracellular niche serves as a barrier for natural genetic exchange via horizontal gene transfer (HGT), and further limits the development and application of genetic tools. To date, the only example for recent inter-phylum HGT among the Chlamydiota is tetracycline resistance in the potentially zoonotic species Chlamydia suis, a close phylogenetic relative of human C. trachomatis, which causes bacterial sexually transmitted infections and ocular trachoma. Tetracycline resistance in porcine C. suis strains has been described worldwide and is always part of a genomic island dividing invasin (inv), located within a chromosomal region between the rRNA operon (rrn) and the nqrF reductase. Here, we aimed to expand the still modest number of available genetic manipulation systems for Chlamydia by generating allele-replacement and integration vectors for C. suis. These vectors comprised homologous C. suis sequences of the chromosomal region of interest, an E. coli origin of replication (ori) and selection markers but lacked the native chlamydial plasmids or its ori. We first recovered allele-replacement mutants using a vector that targets the tryptophan (trp) operon of C. suis. The vector was further successfully maintained as a free plasmid in C. trachomatis without allele replacement, suggesting complex plasmid dynamics in the absence of a chlamydial ori. Moreover, we showed that the hypervariable rrn-nqrF intergenic region of C. suis is highly susceptible to transformation, resulting in complete vector integration upstream of nqrF without interruption of the targeted inv gene.IMPORTANCEThe obligate intracellular Chlamydia genus contains many pathogens with a negative impact on global health and economy. Despite recent progress, there is still a lack of genetic tools limiting our understanding of these complex bacteria. This study provides new insights into genetic manipulation of Chlamydia with the opportunistic porcine pathogen Chlamydia suis, the only chlamydial species naturally harboring an antibiotic resistance gene, originally obtained by horizontal gene transfer. C. suis is transmissible to humans, posing a potential public health concern. We report that C. suis can take up vectors that lack the native plasmid, a requirement for most chlamydial transformation systems described to date. Additionally, we show that C. trachomatis, the most common cause for bacterial sexually transmitted infections and infectious blindness worldwide, can be transformed with C. suis vectors. Finally, the chromosomal region that harbors the resistance gene of C. suis is highly susceptible to complete vector integration.

Exploring the complexities of swapping the order of integration in double integrals

This article explores the conceptual challenges that engineering students encounter with double integrals in a vector calculus course. Drawing on previous literature and utilising APOS (Activity- Process- Objects- Schema) as a theoretical framework, this study investigates the difficulties that students face in understanding and applying double integrals. By analysing responses to a written test that assesses students’ conceptual understanding of double integrals and student responses in interviews, this research sheds light on specific areas where students struggle, such as curve sketching, identifying regions of integration and changing the order of integration. Swapping the order of integration in double integrals is the focus of this research study. The findings of this study have the potential to contribute to the enhancement of teaching and learning strategies for double integrals to better support engineering students’ conceptual comprehension and proficiency in this important mathematical concept.

Nonlocal half-ball vector operators on bounded domains: Poincaré inequality and its applications

This work contributes to nonlocal vector calculus as an indispensable mathematical tool for the study of nonlocal models that arises in a variety of applications. We define the nonlocal half-ball gradient, divergence and curl operators with general kernel functions (integrable or fractional type with finite or infinite supports) and study the associated nonlocal vector identities. We study the nonlocal function space on bounded domains associated with zero Dirichlet boundary conditions and the half-ball gradient operator and show it is a separable Hilbert space with smooth functions dense in it. A major result is the nonlocal Poincaré inequality, based on which a few applications are discussed, and these include applications to nonlocal convection–diffusion, nonlocal correspondence model of linear elasticity and nonlocal Helmholtz decomposition on bounded domains.

Non-expansive matrix number systems with bases similar to certain Jordan blocks

We study representations of integral vectors in a number system with a matrix base M and vector digits. We focus on the case when M is equal or similar to Jn, the Jordan block with eigenvalue 1 and dimension n. If M=J2, we classify all digit sets of size two allowing representation for all of Z2. For M=Jn with n≥3, we show that a digit set of size three suffice to represent all of Zn. For bases M similar to Jn, n≥2, we construct a digit set of size n such that all of Zn is represented. The language of words representing the zero vector with M=J2 and the digits (0,±1)T is shown not to be context-free, but to be recognizable by a Turing machine with logarithmic memory.

On the spurious effects in Lamb-vector-based force decomposition methods

Recent methods based on Lamb vector integration allow for a far-field formulation and decomposition of the whole aerodynamic force, with identification of local flow structures associated with the force generation. Different exact Lamb-vector-based force formulations were proposed in the past and the decomposition of total drag into lift-induced and parasite contributions was subject of very recent studies. However, inconsistencies were also reported, raising questions on the physical role of a non-zero induced drag found in two-dimensional flows. Moreover, the effect of the arbitrary pole (appearing in Lamb-vector-based formulas) on the numerical accuracy of such methods was never investigated. Additionally, the separation of parasite drag into viscous and wave drag contributions by Lamb vector field analysis is still questioned. In this paper, the accuracy of different force formulas and of the drag decomposition in lift-induced and parasite contributions is first analyzed, for steady flows. Two-dimensional numerical solutions around RAE2822 and NACA0012 airfoils are considered, the latter in both inviscid and viscous flows (at low and high Reynolds number), to analyze consistency between lift-induced/parasite drag definitions and the drag generated in isentropic flow conditions (reversible drag) or due to entropy production (irreversible drag). A spurious induced drag contribution (related to the streamwise momentum perturbation) is identified, resulting in negative induced drag values in two-dimensional flows. The effect of a shift in the pole location is also analysed, with reference to its impact on the total force and on drag decomposition accuracy, then a new approach to parasite drag breakdown into physical constituents (viscous and wave contributions) is proposed and results are compared against predictions obtained using consolidated thermodynamic-based methods and against available results from recent literature.

Integrome signatures of lentiviral gene therapy for SCID-X1 patients.

Lentiviral vector (LV)-based gene therapy holds promise for a broad range of diseases. Analyzing more than 280,000 vector integration sites (VISs) in 273 samples from 10 patients with X-linked severe combined immunodeficiency (SCID-X1), we discovered shared LV integrome signatures in 9 of 10 patients in relation to the genomics, epigenomics, and 3D structure of the human genome. VISs were enriched in the nuclear subcompartment A1 and integrated into super-enhancers close to nuclear pore complexes. These signatures were validated in T cells transduced with an LV encoding a CD19-specific chimeric antigen receptor. Intriguingly, the one patient whose VISs deviated from the identified integrome signatures had a distinct clinical course. Comparison of LV and gamma retrovirus integromes regarding their 3D genome signatures identified differences that might explain the lower risk of insertional mutagenesis in LV-based gene therapy. Our findings suggest that LV integrome signatures, shaped by common features such as genome organization, may affect the efficacy of LV-based cellular therapies.

Legal security of the system of infrastructure support of innovative activities under the conditions of European integration processes

Problem setting. The European integration vector of the development of Ukraine in modern conditions should be directed to the key needs of the state, one of which is the aspiration to join the EU, which should be based on established processes of approximation of current legislation to international legal standards and dynamism of entry into the European scientific, educational and digital spaces. Martial law outlined new priorities for state support, however, the implementation of innovative activities is of key importance for ensuring the competitiveness of the national economy, the innovative path of which is actualized precisely through the relevant strategic orientations and measures, which must be of a systemic nature. analysis of recent researches and publications. Legal issues of implementation of support for innovative activities and problems of infrastructural support of the innovation process have been studied by many scientists. In the scientific works of S. V. Hlibko topical issues regarding the improvement of the legal regulation of the innovation process were investigated. O. V. Rozghon conducted a study of conceptual provisions regarding the substantiation of the essence of the category “international technology transfer” and a bibliographic analysis of the study of this category. Yu. M. Zhornokui analyzed legislative and doctrinal approaches in European countries regarding the formation of organizational and legal forms of venture investment in innovative activities. the purpose of the research is to study the topical legal issues related to ensuring the systematic nature of infrastructure support for innovation in the context of European integration processes. article’s main body. In today’s conditions of intensification of integration and globalization processes, one of the main factors in increasing the competitiveness of Ukrainian enterprises is their capacity for innovative activity, the economic effect of which is positive only in the case of the systemic nature of the implementation of innovative changes based on e formed innovation strategy. Proper strategic planning and systematic measures to support activities in the innovation market have a decisive influence on the state of the national economy, including its innovative direction. Today, there are indeed legal problems regarding the lack of a systematic approach to ensuring the innovative development of the national economy and regions of Ukraine, which require an urgent solution in the conditions of the general trend regarding the passive-static state of the current legislation in the field of innovation, taking into account the need for the intensification of European integration processes. The legal support of the national innovation ecosystem should have effective means of supporting and stimulating the implementation of innovative activities and the corresponding infrastructural support of the innovation process, taking into account modern challenges, real needs and generally defined strategic guidelines of the state in this area. At the same time, the construction of an effective innovation policy should also take place according to the principle of adaptive formation, taking into account the real needs of regulatory regulation, the current state of innovation development, the need to ensure effective interaction between all subjects of the innovation infrastructure and components of the national innovation system. conclusions and prospects for the development. The study of current legal issues regarding the provision of systematic infrastructural support for innovative activities in the context of European integration processes allows us to conclude that a dynamic push is now urgently needed for the development of all components of the country’s general innovation ecosystem, a decisive role is played by the use of a systemic approach, which will provide the opportunity to optimize the use innovation potential of Ukraine and significantly increase the competitiveness of the national economy based on the construction of an effective national innovation ecosystem.

Legal security of the system of infrastructure support of innovative activities under the conditions of European integration processes

Problem setting. The European integration vector of the development of Ukraine in modern conditions should be directed to the key needs of the state, one of which is the aspiration to join the EU, which should be based on established processes of approximation of current legislation to international legal standards and dynamism of entry into the European scientific, educational and digital spaces. Martial law outlined new priorities for state support, however, the implementation of innovative activities is of key importance for ensuring the competitiveness of the national economy, the innovative path of which is actualized precisely through the relevant strategic orientations and measures, which must be of a systemic nature. analysis of recent researches and publications. Legal issues of implementation of support for innovative activities and problems of infrastructural support of the innovation process have been studied by many scientists. In the scientific works of S. V. Hlibko topical issues regarding the improvement of the legal regulation of the innovation process were investigated. O. V. Rozghon conducted a study of conceptual provisions regarding the substantiation of the essence of the category “international technology transfer” and a bibliographic analysis of the study of this category. Yu. M. Zhornokui analyzed legislative and doctrinal approaches in European countries regarding the formation of organizational and legal forms of venture investment in innovative activities. the purpose of the research is to study the topical legal issues related to ensuring the systematic nature of infrastructure support for innovation in the context of European integration processes. article’s main body. In today’s conditions of intensification of integration and globalization processes, one of the main factors in increasing the competitiveness of Ukrainian enterprises is their capacity for innovative activity, the economic effect of which is positive only in the case of the systemic nature of the implementation of innovative changes based on e formed innovation strategy. Proper strategic planning and systematic measures to support activities in the innovation market have a decisive influence on the state of the national economy, including its innovative direction. Today, there are indeed legal problems regarding the lack of a systematic approach to ensuring the innovative development of the national economy and regions of Ukraine, which require an urgent solution in the conditions of the general trend regarding the passive-static state of the current legislation in the field of innovation, taking into account the need for the intensification of European integration processes. The legal support of the national innovation ecosystem should have effective means of supporting and stimulating the implementation of innovative activities and the corresponding infrastructural support of the innovation process, taking into account modern challenges, real needs and generally defined strategic guidelines of the state in this area. At the same time, the construction of an effective innovation policy should also take place according to the principle of adaptive formation, taking into account the real needs of regulatory regulation, the current state of innovation development, the need to ensure effective interaction between all subjects of the innovation infrastructure and components of the national innovation system. conclusions and prospects for the development. The study of current legal issues regarding the provision of systematic infrastructural support for innovative activities in the context of European integration processes allows us to conclude that a dynamic push is now urgently needed for the development of all components of the country’s general innovation ecosystem, a decisive role is played by the use of a systemic approach, which will provide the opportunity to optimize the use innovation potential of Ukraine and significantly increase the competitiveness of the national economy based on the construction of an effective national innovation ecosystem.

The use of lexical retrieval strategies by creative second language learners: A computational analysis of clustering and switching

This study seeks to delve into the potential role of divergent thinking, a component of creativity, in second language learning. Specifically, we compare the use of lexical organization and production strategies of two groups of more and less creative EFL learners in year 12 through an automatic vectorial semantic analysis of their retrieval in three second language semantic fluency tasks. Consistent with previous research in the field of creativity, our findings indicate that the creative group retrieved more second language words than the less creative group. These words were less related to each other and to the stimulus categories than the words generated by the less creative group. While the creative participants’ retrieval was based on an extensive use of switching, a slight but non-significant trend was found in the production of longer clusters by the less creative participants. These results yield interesting insights into the potential role of creativity in second language learning.

Learning from Lecture and Homework: The Case for Studying Intersections of Milieu

ABSTRACT In an online asynchronous vector calculus course, we observed exam answers solved with a formula from online homework instead of the formula from lecture. Our exploratory study investigated (1) why students learned from homework instead of lecture for this topic and (2) their epistemological frames (e-frames) for lecture and homework. Per (1), reasons included studying homework as more efficient than reviewing lectures, difficulty understanding the lecture, and incomplete lecture notes. Per (2), some students see lecture as explaining mathematical meaning and giving them tools to do homework. Students primarily see homework as a space to learn procedures. The results are significant for several reasons. First, they connect homework and lecture learning, rather than treating them separately (as in prior research). A key contribution of the work is both the empirical documentation that students’ lecture and homework learning influence one another, and the implication from this that researchers should attend to learning at the intersections of milieu. A second significance of the work is the preliminary documentation of students’ e-frames for lecture and homework. Characterizing e-frames is important because improvements in student learning may come in part from helping students shift their beliefs about learning and about mathematics as a discipline.

Contour integration for the view factor calculation between two rectangular surfaces

AbstractThe calculation of the view factor is an important consideration in radiative heat transfer analysis. Thermal engineering requires the view factor calculations between complex geometries containing parallel and inclined surfaces. There is currently no analytic formula available for this, so the summation rule (SR) must be used. However, when complex geometries are analyzed, processing SR becomes a very intensive task. Therefore, the present study aims to exploit vector calculus to develop a formula for calculating the view factors for radiative heat transfer from any two rectangular oblique or parallel surfaces. The exact solution obtained by means of the contour integration was compared with the numerical integration of the quadruple integral using the multiple Simpson's rule 1/3 with five intervals (MSR) and the summation rule via the Hamilton–Morgan solution (HMS). The lack of similar precedents in the literature reinforces the scientific and practical nature of the proposed method.

Myelodysplasia after clonal hematopoiesis with APOBEC3-mediated CYBB inactivation in retroviral gene therapy for X-CGD

Stem cell gene therapy using the MFGS-gp91phox retroviral vector was performed on a 27-year-old patient with X-linked chronic granulomatous disease (X-CGD) in 2014. The patient's refractory infections were resolved, whereas the oxidase-positive neutrophils disappeared within 6months. Thirty-two months after gene therapy, the patient developed myelodysplastic syndrome (MDS), and vector integration into the MECOM locus was identified in blast cells. The vector integration into MECOM was detectable in most myeloid cells at 12months after gene therapy. However, the patient exhibited normal hematopoiesis until the onset of MDS, suggesting that MECOM transactivation contributed to clonal hematopoiesis, and the blast transformation likely arose after the acquisition of additional genetic lesions. In whole-genome sequencing, the biallelic loss of the WT1 tumor suppressor gene, which occurred immediately before tumorigenesis, was identified as a potential candidate genetic alteration. The provirus CYBB cDNA in the blasts contained 108 G-to-A mutations exclusively in the coding strand, suggesting the occurrence of APOBEC3-mediated hypermutations during the transduction of CD34-positive cells. A hypermutation-mediated loss of oxidase activity may have facilitated the survival and proliferation of the clone with MECOM transactivation. Our data provide valuable insights into the complex mechanisms underlying the development of leukemia in X-CGD gene therapy.

СУЧАСНІ ПІДХОДИ ДО ФОРМАТИЗАЦІЇ РОЗДРІБНИХ ТОРГОВЦІВ

In this article, the authors specify the main classification features of retail trade enterprises at the current stage of their development, and also provide the main characteristics, principles and approaches to the definition (formation) of modern formats of retailers (formatting). Thus, it was determined that there are five main types of retailers, within which a number of different formats operate. The definition of the format of a retailer almost corresponds to the definition of its format, but is broader and characterizes in more detail the main indicators of a retail trade enterprise, such as forms and methods of trade service, price and assortment policies of the enterprise, its size and status, affiliation, etc. The authors also analyzed and compared formatting parameters of retailers in European and American countries in the article. It was determined that the main parameters that determine the format of a retailer in America are characterized by much higher indicators than in European countries. Based on the analysis, the authors identified a number of common features and deviations in the process of formatting retailers in Europe and America and their classification, as well as proposed specific criteria and approaches to the formatting of retailers in Ukraine at the current stage. Taking into account the European integration vector of the development of the national economy of Ukraine and in order to ensure a quick and high-quality transition of Ukraine to the European standards of trade service and unification, the authors recommend implementing the European model into the domestic practice of formatting retail trade enterprises.

Geopolitical «Borderliness» as a Factor in Ukraine’s Positioning in International Relations: the Experience of Ukrainian Discourse at the Beginning of the XXI Century

The article considers the problem of the phenomenon of geopolitical «borderliness» as a factor in the positioning of Ukraine in international relations within the Ukrainian scientific discourse of the early XXI century. The definition of the concept of geopolitical «borderliness» has been presented. It is shown that Ukraine is an average regional power, which is characterized by a «borderliness» geopolitical position. On the modern Ukrainian territory, the Euro-Atlantic, Eurasian, Islamic massif and, accordingly, the «super-ethnic frontiers» of the Western European, Slavic and Islamic civilizations intersect. Concepts of the genesis of ideological and sociocultural differences within Ukrainian society in the regional dimension have been characterized. It is substantiated that the foreign policy orientation of Ukraine has always been a derivative of the interaction of the Euro-Atlantic, Eurasian and Black Sea (South-Eastern) paradigms, which necessitates adherence to the «orientation priority» in international political positioning. It follows from this that it is expedient for the Ukrainian state to be guided by a non-bloc strategy. According to Ukrainian researchers, the entry of Ukraine into allied relations with one of the competing poles can lead to a strategic conflict with another center of power and negative consequences for Ukrainian statehood. To prevent this from happening, Ukraine was recommended to pursue a foreign policy on the basis of a neutral status and the principles of «optimal multi-vector approach». This was confirmed by the Ukrainian sociological research. The geopolitical and socio-cultural «borderliness» determines the «split» of Ukrainian society, which is manifested in the foreign policy orientations of residents of different regions. According to Ukrainian sociologists, traditionally the majority of the population of the West of Ukraine and about half of the Center supported the European integration vector. At the same time, about half of the inhabitants of the Center and the majority of the population of the South and East of Ukraine were supporters of the development of relations with Russia and the Eurasian vector of integration. As the only option for foreign policy positioning that would unite the Ukrainian society of different regions, the achievement of national agreement through a compromise was noted, this was possible only if Ukraine pursued a policy of neutrality and «optimal multi-vector approach».

Orthogonal signed-distance coordinates and vector calculus near evolving curves and surfaces

We provide an elementary derivation of an orthogonal coordinate system for boundary layers around evolving smooth surfaces and curves based on the signed-distance function. We go beyond previous works on the signed-distance function and collate useful vector calculus identities for these coordinates. These results and provided code enable consistent accounting of geometric effects to arbitrary order for boundary layer asymptotics in a wide range of physical systems.

Analysis of Some Biochemical Properties of Recombinant Siberian Roe Deer (Capreolus pygargus) Chymosin Obtained in the Mammalian Cell Culture (CHO-K1).

Structure of the chymosin gene of Siberian roe deer (Capreolus pygargus) was established for the first time and its exon/intron organization was determined. Coding part of the chymosin gene of C. pygargus was reconstructed by the Golden Gate method and obtained as a DNA clone. Comparative sequence analysis of the roe deer, cow, and one-humped camel prochymosins revealed a number of amino acid substitutions at the sites forming the substrate-binding cavity of the enzyme and affecting the S4 and S1' + S3' specificity subsites. Integration vector pIP1 was used to construct a plasmid pIP1-Cap in order to express recombinant roe deer prochymosin gene in CHO-K1 cells. CHO-K1-CYM-Cap pool cells were obtained, allowing synthesis and secretion of recombinant prochymosin into the culture fluid. As a result of zymogen activation, a recombinant roe deer chymosin was obtained and its total milk-clotting activity was estimated to be 468.4 ± 11.1 IMCU/ml. Yield of the recombinant roe deer chymosin was 500 mg/liter or ≈468,000 IMCU/liter, which exceeds the yields of genetically engineered chymosins in most of the expression systems used. Basic biochemical properties of the obtained enzyme were compared with the commercial preparations of recombinant chymosins from one-humped camel (Camelus dromedarius) and cow (Bos taurus). Specific milk-clotting activity of the recombinant chymosin of C. pygargus was 938 ± 22 IMCU/mg, which was comparable to that of the reference enzymes. Non-specific proteolytic activity of the recombinant roe deer chymosin was 1.4-4.5 times higher than that of the cow and camel enzymes. In terms of coagulation specificity, recombinant chymosin of C. pygargus occupied an intermediate position between the genetically engineered analogs of B. taurus and C. dromedarius chymosins. Thermostability threshold of the recombinant roe deer chymosin was 55°C. At 60°C, the enzyme retained <1% of its initial milk-clotting activity, and its complete thermal inactivation was observed at 65°C.

DANCE TRADITIONS IN THE SCIENTIFIC DISCOURSE OF YOUTH CULTURE

We consider dance traditions in the scientific discourse of youth culture in the field of choreographic activity (culture, education, leisure) as a means of youth culture. Dance traditions require attention from the scientific discourse of cultural studies. Therefore, we consider traditions as an integral component of Ukrainian culture, education, and recreational institutions. An overview of publications is presented, in which traditions and choreographic activity are analyzed directly in the scientific discourse. The role of dance in promoting the optimization of cultural communication, the acquisition of knowledge, the study and assimilation of samples of national culture, and the education of young people through the means of culture is analyzed. In particular, the role of educational institutions and institutions of culture and arts as centers for providing cultural requests of youth is sporadically highlighted. It was revealed that the culture process directs the youth to the European integration vector with its orientation to the future, the transmission of traditions in the plastic manifestations of folk art to future generations, the assimilation of values and priorities of cultural ideals. The implementation of traditions in choreography involves factors of a complex nature: training of professional dance disciplines in the cultural context of humanitarian sciences : philosophy, aesthetics, ethnocultural studies, art history. The complex character solves the leading tasks of art education, in which culture plays a leading role, so young people focus on the formation of special knowledge, practical skills and skills for improving special techniques and performing skills.

Safe and efficient lentiviral vector integration with HSC-targeted gene therapy.

Safe and efficient lentiviral vector integration with HSC-targeted gene therapy.