Intermittent pneumatic leg compression (IPC) therapy is commonly used to treat symptoms associated with peripheral artery disease. One hypothesis is that IPC increases shear rate (SR) in the arteries and enhances endothelial cell (EC) function. To test this hypothesis we characterized the hemodynamic profile and used flow mediated dilation (FMD) to assess EC function in response to a single session of high frequency (HF) and low frequency (LF) IPC. Eleven male subjects (age: 23.5 ± 4.7 years) underwent an hour of HF (2s inflation/3s deflation) and LF (4s inflation/16s deflation) IPC treatment on separate days. Doppler/ultrasonography of the popliteal artery (PA) was used to measure diameter and blood velocity at the 5th and 45th minute of treatment and to assess FMD pre and post treatment. IPC had no effect on PA FMD (HF: pre: 2.45±.50%, post: 2.76 ±.32%; LF: pre: 3.16±.36%, post: 3.56±.52%). Cuff deflation elevated blood flow (BF) and SR and decreased oscillatory shear index (OSI) from baseline at the onset of both treatments (p<.01). This effect was no longer evident by the 45th minute of HF treatment only. When compared to baseline both treatments significantly reduced BF and SR and increased OSI during cuff inflation (p<.01). In conclusion, a single session of IPC does not acutely impact PA FMD despite significant alterations to the hemodynamic profile during treatment. HL35088 (MHL, BTR); ACSM Research Endowment (SCN)