This study evaluates the potential of thermosyphon for passive cooling of nuclear fuel storage vaults. For this work, a thermosyphon was specifically designed and manufactured. Experiments were conducted by varying heat load (300–600 W) and filling ratio (40–120 %). Optimal performance was obtained with a 60 % filling ratio. The experimental data obtained with the optimal filling ratio were used to calculate the effective thermal conductivity of thermosyphon and determine the number of thermosyphons needed for the vault. Two geometric vault ventilation models; one featuring a thermosyphon and the other without these were developed. The operations of these vault ventilation systems were simulated using the CFD model. The airflow patterns and temperature distributions within the storage vault were compared. The findings ascertain the utility of thermosyphons as an effective passive cooling device in the nuclear fuel storage vault.