This study focuses on the effect of opening on the performance of bamboo-reinforced concrete wall panels subjected to one-way in-plane action. The study evaluates the performance of six bamboo-reinforced concrete wall panels, of which three are solid wall panels and the remaining three specimens are having an opening at its center. All the prototype specimens were subjected to a uniformly distributed load that was applied at an eccentricity of 1/6 of its wall thickness. The specimens considered in this study had a constant slenderness ratio of 25, with varying aspect ratios and thinness ratios. The aspect ratio considered for the wall panels were 1, 1.20, and 1.52, and thinness ratios considered include 16.50, 20.75, and 25. The area of the opening provided was exactly 25% of the area of the specimen and was provided at the center of the specimen. The study reveals that the load-carrying capacity of a wall panel is dependent on its aspect ratio and thinness ratio. The ultimate load was found to decrease with an increase in aspect ratio, whereas it increased with increase in thinness ratio irrespective of the presence of the opening.