Various Types Of Pathology Research Articles

Leader of pathophysiology: on the 70th birthday of professor Vasily N. Tsygan

May 12, 2024, marked the 70th birthday of the Honored Scientist of Russia, corresponding member and honorary doctor of the Kirov Military Medical Academy, Prof. Vasily N. Tsygan. He is a brilliant embodiment of the traditions of academic scientific schools, having gone from being a member and chairman of the Military Scientific Society of Cadets and Students to an adjunct to the deputy head of the academy for scientific work. After graduating from the academy with a gold medal and serving 3 years in the military, Vasily N. Tsygan entire subsequent scientific, pedagogical, and organizational activity has been connected with the academy. The topic of his candidate dissertation, related to changes in homeostasis in mechanical trauma, and performing special assignments from the command in Afghanistan and in the zone of the counter-terrorist operation in the Chechen Republic determined a crucial vector of research into the extreme impacts on the human body during military professional activity. V.N. Tsygan contributed to the study of immunophenotyping problems, the modern concept of molecular remission in oncohematology, and immunodeficiency in various types of pathology and methods for their correction. He is an experienced organizer of science and many large international scientific events. His activities involved the study of the history of Russian medicine, relations with the Nobel Committee, and defending the priority of Russian scientists. V.N. Tsygan’s contribution to the creation and development of the Military Medical Academy journals, where he worked for decades as executive secretary and deputy editor-in-chief, is invaluable. The Academy, the Ministry of Defense, the Academy of Medical Sciences, and the state have highly appreciated the merits of Prof. V.N. Tsygan, honoring him with state and departmental awards, prizes, and honorary titles.

Gene expression of antimicrobial peptides in rat intestine under conditions of chronic stress

BACKGROUND: Severe stress causes an array of dysfunctions in the immune, neuroendocrine, cardiovascular, digestive and other systems, resulting in an emergence of various types of pathology. Common manifestations of a chronic stress are the disorders in the gastrointestinal tract, such as irritable bowel syndrome, functional dyspepsia, biliary dyskinesia, dysbiosis, inflammatory processes that determine the development of gastritis and one of the most widespread post-stress pathologies of the gastrointestinal tract — stomach ulcers. The disclosure of the molecular mechanisms of a pathogenesis of diseases associated with gastrointestinal dysfunction related to chronic stress as well as a search for new ways to correct these disorders are important tasks of fundamental and clinical medicine. The present work is focused on evaluating a participation of molecular factors of the innate immunity in intestine, such as antimicrobial peptides secreted by intestinal epithelial cells upon infection, in a response to the chronic stress. AIM: The aim of the study was to estimate the gene expression of a number of antimicrobial peptides: intestinal α- and β-defensins of laboratory animals (rats) under chronic stress conditions. MATERIALS AND METHODS: Modeling of a chronic stress was performed by daily forced swimming of laboratory animals in cold water. An expression of α- and β-defensin genes was evaluated using a real-time polymerase chain reaction. RESULTS: We found an increase in the level of expression of the rat α-defensin-5 and β-defensin-3 genes in response to chronic stress, while the expression of β-defensin-2 gene was not changed compared to the control. CONCLUSIONS: Considering that changes in the concentration and spectrum of peptides with antibacterial activity, caused by prolonged stress, can contribute to modification of the composition of the intestinal microbiota, the data obtained can expand our understanding of the molecular basis of the pathogenesis of diseases associated with disorders in the composition of microbiota under stress.

Задний сегмент глазного яблока: клинический случай хронической центральной серозной хориоретинопатии

Central serous chorioretinopathy is most often found in able-bodied men of young and middle age. The aim of the study: to improve the quality and efficiency of medical care for patients with chronic central serous chorioretinopathy. A clinical example. Patient D., 46 years old, applied in May 2022. Treatment of this pathology is carried out according to the standards of specialized medical care in the field of ophthalmology, approved by the Ministry of Health of the Russian Federation. The clinical anatomy of the posterior segment of the eyeball is the basis for assessing various types of pathology, including chronic central serous chorioretinopathy. Diagnostics with visualization of layers in the area of the posterior segment of the eyeball is predominant among the research methods. Treatment in accordance with the standards of medical care leads to the restoration of the anatomical noma of the structures of the posterior segment of the eyeball. Keywords: posterior segment of the eyeball, human anatomy, central serous chorioretinopathy

Особливості диференціювання хірургічної патології органів черевної порожнини і тазових органів у дівчат за допомогою ультразвукового дослідження

The issue of acute appendicitis remains extremely relevant today, as it is the most common inflammatory disease of the abdominal cavity. Despite significant advances in pediatric surgery in recent decades, when mortality from acute appendicitis is an exceptional event, the number of complicated forms in children is observed in 26.4% of cases, and morphologically unconfirmed appendectomies in 8.8% of cases. In girls, the rates are higher at the age of ≤10 years. Acute appendicitis has been and remains a very common disease in underdeveloped countries. In industrialized countries, the incidence of acute appendicitis ranges from 4-6% to 12-23%, in Ukraine - 20-21 cases per 10 thousand population, mostly young people. Purpose - to state the peculiarities of differentiation of surgical pathology of the abdominal cavity and pelvic organs in girls using ultrasound examination; to develop an algorithm for diagnosing surgical pathology in girls. Materials and methods. The study was conducted on the basis of the at the Vinnytsia Regional Children's Clinical Hospital, National Pirogov Memorial Medical University (Vinnytsia, Ukraine) for the period 2019-2021. For visual differential diagnosis, girls aged 4-16 years were involved in the study: 100 healthy children (the Group 1), 100 children diagnosed with acute phlegmonous appendicitis (the Group 2), 100 children with bulky uterine appendages of various types of pathology (the Group 3). Results and conclusions. The following advantages of ultrasound have been established: high specificity of the study, low cost and absence of ionizing radiation. Ultrasound of the abdominal cavity makes it possible to diagnose acute surgical and gynecological pathology, as well as to carry out differential diagnosis of diseases simulating acute appendicitis. An algorithm for diagnosing surgical pathology in girls has been developed. Such an algorithm increases the effectiveness of ultrasound of acute appendicitis and neoplasms of the pelvic cavity, and this allows the doctor to establish the correct diagnosis in time and choose the appropriate treatment tactics. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.

Analysis of the microelement composition of saliva in patients with comorbidities

The microelement composition of saliva makes it possible to analyze the processes occurring in the oral cavity and the body as a whole, both under conditions of normal functioning and in the presence of pathological processes. At the same time, the technical simplicity of performing trace element analysis of salivary fluid as a possible diagnostic criterion for various diseases contributes to a significant number of studies devoted to the search for possible correlations between the trace element composition of salivary fluid and various types of pathologies. The possibility of using the analysis of the microelement composition of saliva in determining concomitant pathologies of organs and systems of the body as a whole is a promising direction in scientific research, including applied research. The article analyzes the possibilities of using microelement analysis of saliva in such pathologies as psoriasis, Parkinson's disease, schizophrenia, pathology of the gastrointestinal tract, diabetes mellitus, pathology of the cardiovascular system, giardiasis, oncological diseases. It is noted that at present, the determination of the microelement composition of saliva in various pathological conditions is considered as a promising clinical direction that allows for the simultaneous diagnosis of several diseases in real time.

Physical therapy during the Great Patriotic War

The article presents an analysis of the application of physical therapy methods during the Great Patriotic War in the Soviet Union. It was observed that physical therapy, combined with surgical and other methods, significantly improves the efficacy of patients' treatment, including with battle injuries, as well as with injuries of other etiology, such as frostbite, and also contributes to reducing the incidence of disabilities among the injured. The features of the physical therapy methods' organization sequently from 1941 to 1945, and also the results of active development of physical medicine, have been characterized. The features of physical therapy methods' application both on different bases of healthcare delivery (hospital bases, in the rear) and with regard to various types of pathology have been shown. The importance of involving physical therapy methods in curative measures from the early stages of treatment has been noted.

Cross sectional study on cytomorphological diagnosis and clinical presentation of various thyroid diseases in Tribal areas of Odisha

Introduction: Thyroid is an endocrine gland which is affected by various types of pathology including non-neoplastic and neoplastic conditions. The disease burden is very high in costal areas of India and Himalaya areas. Fine needle aspiration cytology (FNAC) is an important mode of initial diagnosis of various diseases of the gland. The study was conducted in various tribal areas of Odisha to find various clinical features and cytomorphological (FNAC) finding of various pathology of thyroid. Methodology: The study included total 210 cases of thyroid disorder over a period of 1 year from October 2019 to September 2020 in the department of pathology. Cytomorphological diagnosis by FNAC and brief clinical data were taken into consideration for statistical analysis. Result: This study revealed that female cases were 86.2 % and male 13.8 % and highest percentage (44.28 %) of patients were in age group 21 to 40 years. Other than gland enlargement three most common presentations were heat intolerance, cold intolerance and tachycardia.

Molecular mechanisms of molecular transfer across the blood-brain barrier as a target for pharmacological action Part 1. Structure, function and pathology of the BBB

Biological barriers play a key role in maintaining the integrity and functioning of the body at all levels of its organization. The barrier function at the cellular level is based on the hydrophobic properties of the cytoplasmic membrane, which provide selective permeability for various substances, depending on their chemical properties. At higher levels of organization, from tissue to organism, the barrier function is based on intercellular protein complexes of the plasma membrane, which form paracellular diffusion barriers and separate internal and external fluid media, which is a necessary condition for the development and functioning of each organ. The blood-brain barrier (BBB) ​​plays an important role in maintaining the function of the brain. The review reflects the main stages in the embryonic development of the BBB, as well as presents current data on the morphological and functional features of the organization of the BBB, including molecular mechanisms that mediate the barrier function due to the complex participation of vascular cells of cerebral microvessels, as well as gene expression of enzyme complexes, active and passive substance transport mechanisms through the BBB. The high transport selectivity of the BBB is an urgent problem for the delivery of drugs to the brain. At the same time, it is equally important to improve the principles of pharmacotherapy for the correction of impaired BBB functions in various types of pathology of the nervous and other body systems. This review aims to convey to the developers of modern targeted drugs new information about the molecular genetic mechanisms of the transport of substances through the BBB, as well as to draw the attention of specialists in the field of precision medicine to the problem of violations of the barrier function of cerebral vessels in neurological and other diseases of a modern person.

Mechanisms of Modulating Action of Low-Intensity Laser Radiation on the Proliferative Activity of Cells in the Stromal and Vascular Fraction of Adipose Tissue

The efficacy of low-intensity laser radiation therapy using autologous cellular products based on adipose tissue, including stromalvascular fraction, has been shown to be effective in different forms of pathology and in reconstructive and plastic surgery. The review deals with the issues related to the methodological aspects of the stromal and vascular fraction isolation from the human adipose tissue as well as with the peculiarities of its application in experimental studies on animal models and in clinical practice. The mechanisms of low-intensity laser radiation effect on the cells of stromal-vascular fraction has been discussed. An analysis of recently published research results on the effects of laser radiation on mesenchymal stromal cells of adipose tissue is carried out. The results of experimental studies on the choice of optimal modes and parameters of low-intensity laser radiation with the aim of their application in complex cell technologies are discussed. The issues related to the therapeutic effects of low-intensity physical factors in autologous transplantation of the stromal-vascular fraction and mesenchymal stromal cells in various types of pathology are viewed. Further research on the mechanisms of low-intensity laser irradiation effects on various types of cells and tissues is required to expand the clinical application of cell-based therapy

What the time has taught about Hyperteleorbitism

Introduction: Hypertelorbitism is a clinical feature that may be present in various types of pathologies and degrees of manifestation. Observing the evolution of techniques over time and understanding the specific characteristics of each case demonstrates the difficulty of establishing treatment protocols.
 Material and method: Retrospective analysis of over 150 cases of hypertelorbitism operated on over 44 years by the main author. The increased interorbital distance had varied etiologies: dysplasia, meningoencephaloceles, and dysostosis. 
 Results: Retrusion of the facial middle third throughout growth in congenital hypertelorbitism was present in both dysplasia and dysostosis groups. In cases of early treatment with Tessier's glasses osteotomy there was zygomaticmaxillary atrophy with trapping of tooth germs. The cases of grade III and IV Hyperteleorbitism, with or without meningoencephalocele, which were treated without concomitant skullcap expansion, evolved to endocranial hypertension and required further treatment. Retrospective evaluation of their CT scans revealed craniosynostosis of one or more sutures, associated with predominant dysplasia. Patients treated after adolescence showed more stable late postoperative results.
 Discussion: Hypertelorbitism is a clinical feature that may be present in various types of pathologies and in varying degrees of intensity. Many treatment options have been proposed over the past 50 years. The opportunity to watch and experience the evolution of these techniques made it possible to choose the most appropriate treatment for each patient's clinical characteristics.
 Conclusion: Due to the variability of manifestation of hypertelorbitism, the massification of procedures and the establishment of treatment protocols are quite limited.

Chemical Composition and Antioxidant, Anti-Inflammatory and Anticholinesterase Properties of the Aerial and Root Parts of Centaurea acaulis Essential Oils: Study of the Combinatorial Activities of Aplotaxene with Reference Standards

In recent years, growing attention has been given to essential oils. Essential oils obtained from medicinal plants show high therapeutic potential against various types of pathologies, including Alzheimer's, cancer and inflammatory diseases. The purpose of this work was to study the chemical composition of the aerial and root parts of Centaurea acaulis essential oils by GC/FID and GC/MS, to evaluate the anti-inflammatory, antioxidant and neuroprotective properties, and in-vitro combinatory effect of aplotaxene isolated from the roots with reference standards, in order to find new and more effective agents for the treatment of degenerative and inflammatory diseases. The statistical analysis clustered the essential oil aerial parts into two distinct groups. The specimens characterized by a humid climate and low altitude was mainly discriminated by the high contents of (E)-β-caryophyllene (3.4-8.4%), hexadecanoic acid (6.3-10.8%) and caryophyllene oxide (6.2-9.9%). While the second group characterized by a humid climate and higher altitudes was characterized by high levels of limonene (15.2-19.2%) and τ-muurolol (12.6-17.2%). In contrast, essential oils of roots were dominated by aplotaxene whatever the climate type. All samples showed very good antioxidant and anti-inflammatory activities. The combination of aplotaxene with references gave synergistic effects with excellent activities about 2 times higher than the synthetic references. The neuroprotective activity of essential oils and aplotaxene did not show inhibition against AChE, whereas they inhibited BChE with IC50 values comparable to Galantamine.

Exogenous and endogenous mortality parameters as indicators of the sanitary and epidemiological status of the population of the region

Introduction. The concepts of the epidemiological transition and types of population health are applied to analyzing and evaluating the sanitary and epidemiological status of the population. Materials and methods. For many years of mortality, the dynamics of the male population of the Irkutsk region standardized indicators were used (European age standard). For identifying trends of changing mortality rates, an analytical alignment of the time series was used. It was a linear model by EXCEL’s standard tools (Microsoft Office 2007). The significance of the trend equation was calculated using F-test in IBM SPSS Statistics 23 (p < 0,05). Results. It has been established that the formation of various types of pathology and caused by this pathology mortality occurred under the influence of factors of different nature in the process of changes in social, environmental, epidemiological situations in the region. In the 1990 th and the first half of the 2000 th, high mortality rates in the class of diseases of the circulatory system and the class of external causes of morbidity and mortality were determined mainly by psychosocial disadvantage factors. By the class of Diseases of the respiratory system, there was noted a steady decrease in mortality rate from chronic diseases of the lower respiratory tract and pneumonia. It was probably due to an increase in the level and quality of medical care. A steady tendency to increase intensive and extensive mortality rates by class of Certain infectious and parasitic diseases was seen. That was caused by negative changes in infectious and epidemic processes under the influence of man-made pollution was noted. During 2005 - 2015 the digestive system’s share of the class of Diseases in the mortality structure increased. That is due to the prevalence of alcoholic liver disease, chronic alcoholic pancreatitis, complications of the ulcer of the stomach and duodenum. Conclusion. The combination of the identified patterns of the formation of intensive and extensive mortality rates determined by exogenous and endogenous factors made it possible to characterize the situation in the Irkutsk region as a relative sanitary and epidemiological disadvantage of the population.

Reduction of plasmid vector backbone length enhances reporter gene expression

Gene therapy has a wide range of applications for various types of pathologies. Viral methods of gene delivery provide high levels of gene expression but have various safety concerns. Non-viral methods are largely known to provide lower levels of expression. We aim to address this issue by using plasmid DNA with smaller backbones to increase gene expression levels when delivered using non-viral methods. In this study we compare gene expression levels between two vectors with firefly luciferase encoding gene insert using liposome complexes and gene electrotransfer as delivery methods. A 2-fold reduction in plasmid vector backbone size, disproportionately enhanced gene expression levels more than 10-fold in rat tenocytes in vitro, and rat myocardium in vivo, while improvements in delivery to the skin were more moderate.

A worthy technique for transcanal drilling during endoscopic ear surgery

ObjectiveTo evaluate the necessity and effectiveness of a preplanned technique for drilling during transcanal endoscopic ear surgery.MethodsStudy design: Retrospective case series study from June 2011 to June 2015. Setting: Private tertiary care hospital. Patients: Eighty-five ears of 78 patients, age ranging from 9 to 57 years underwent transcanal endoscopic drilling for various types of pathology in their middle and external ear. Interventions: Application of a preplanned technique for transcanal drilling in endoscopic ear surgery that involved short timed drilling with use of intermittent irrigation and suction. Every events of the procedure were done one after another with the single hand of the surgeon. An attachment providing protecting sheath around rotating burr was used during each time of drilling. Main outcomes measure: Efficacy of such drilling technique in single handed endoscopic ear surgery. Presence of any postoperative thermal injury of facial nerve and any lacerated injury of skin of external ear.ResultsThis preplanned technique was found suitable for transcanal endoscopic drilling with the single hand of the surgeon. Postoperative facial nerve palsy or laceration of skin of external ear was not noted in any patient.ConclusionAfter using the present technique, transcanal endoscopic drilling could be done easily and safely with single hand of the surgeon.

Neuroclusterization method of stabilometric data

Stabilometry is a modern method for assessing the functional state of a person by the ability to maintain a stable balance of an upright posture. Technically, the implementation of the stabilometry method consists in measuring, with the help of specialized devices, the values that make up the support reaction, with the subsequent determination, according to these measurements, of the coordinates of the center of body pressure on the support. The nature of the migrations of the center of pressure during the stabilometric study is a source of information about the features of the processes of postural regulation. At the same time, up to the present time, there is a problem of the correct interpretation of the results of stabilometry. The adequacy of the conclusions is largely determined by the human factor, i.e. qualification of a specialist analyzing stabilometry data. Thus, in our opinion, the task of objectifying the assessment of stabilometry results is urgent. The aim of this work is to study the possibility of applying the neurocluster method using self-organizing neural networks to objectify the analysis of stabilometry data. The authors proposed a technique for analyzing the structure of individual and group stabilometric data by clustering them using selforganizing Kohonen neural maps with Euclidean metrics. Neuroclusterization of stabilometric data allows in automatic mode (without human intervention) to identify the type of group of subjects corresponding to the norm or pathology, various types of pathologies, as well as individual biometric characteristics of the subjects. The subsequent analysis of the individual characteristics of the data of the subjects, grouped in this way, makes it possible to detect deviations indicating the presence of abnormalities or the formation of various pathological conditions, which can be useful for the early diagnosis of diseases.

Possibilities of Regular Physical Culture Lessons in Restoring the Functional Status of Students

The high level of development of modern medicine cannot reduce the growing prevalence of various types of pathologies and injuries in people of different ages. Recently, a fracture of the lower jaw is often found among student youth. In this regard, the issue of improving the rehabilitation of young people with this type of injury is relevant. The importance of this problem is associated with the high frequency of such an injury and the increase in the number of complications developing against

Types of Neurosurgical Patients Requiring Tracheostomy- Institution Based Study

Background and purpose: Tracheostomy is common surgical procedure among neurosurgical patients who are ventilater dependent for prolonged period of time in neuro ICU. With the objective to analyzed various type of neurosurgical patients requiring tracheostomy this study was performed.
 Material and method: This is a Crossectional , Analytical stydt with Non probability consecutive sampling over the duration of 2 years. Association between age vs categories of different pathologies and Gender is categories of different neurological pathologies were analysed using Chi square test/fishers exact test wherever applicable using SPSS20.
 Result: There were total of 85 patients who underwent tracheostomy for various types of pathologies in our institute over the duration of two years. Mean age of patients who undergone tracheostomy was 45.26 ( SD 18.9) years and they ranged between 8 and 86 years. Among all these categories head injuries accounted for 45% of cases followed by ICH (28%).
 Conclusion: Head Injury and intracerebral hemorrhage seems to be the common pathology requiring tracheostomy in tertiary neurosurgical centre. Young and male patients are more predominant in this study.

A study of histopathological spectrum of breast lesions in a tertiary care hospital

Background: Various types of pathologies from inflammation to carcinoma are known to affect breast tissue in females. Some lesions are common in young females while others are more common in elderly age group. Accurate diagnosis is essential to relieve anxiety of patients. In case of carcinoma, early and accurate diagnosis can save the patient from metastases, thus reducing mortality and morbidity. Aims and objectives: This study is aimed to analyse the histopathological spectrum of various breast lesions and to study the distribution of breast diseases in different age groups and gender in our institute. Materials and Methods: It is a prospective study performed in the Department of pathology at Government Medical College/Government General Hospital Anantapuramu over a period of four years from January 2015 to December 2018. During this period a total of 310 breast specimens were received in the Department of pathology. Histopathological examination of specimens was done to know the spectrum of breast lesions. Results: Out of 310cases, 290cases were female and 20 cases were male. 185 cases were benign breast neoplasms, 123 cases were malignant neoplasms and 02 cases were inflammatory lesions. Among the 185 cases of benign breast neoplasms, 141 cases were fibroadenoma which was the most common benign breast lesion. Out of 123 malignant breast lesions, 2 cases were Duct cell carcinoma in situ and 121 cases were invasive malignant breast lesions. Out of which 97 cases were Invasive ductal carcinoma –NOS subtype which was the most common lesion. Conclusion: 59.68% of breast lesions were benign breast lesions proving to be the majority in incidence. 39.68 % of lesions were malignant breast lesions and 0.64% were inflammatory lesions. Benign lesions were common in second to fourth decade and malignancy in fifth and sixth decades.

Neutrophil granulocytes: new faces of old acquaintances

Over the past few decades, thanks to the use of new technologies, the spectrum of functional capabilities of neutrophil granulocytes has been significantly expanded. Their effector potential with respect to infectious agents, including phagocytosis, the production of active forms of oxygen and nitrogen, degranulation with the release of numerous enzymes and antimicrobial peptides, and the formation of extracellular traps were studied in detail. However, it has been found that many of the factors that neutrophils use to directly destroy pathogens have a regulating effect on other cells of the immune system and the neutrophils themselves. In addition, upon activation, neutrophils are capable of synthesizing a number of de novo biologically active molecules. Traditionally considered as inducers of an inflammatory reaction, neutrophils demonstrate the ability to simultaneously incorporate mechanisms that contribute to limiting and resolving inflammation. Ambivalent both helper and suppressor effects of neutrophils on cells of congenital and adaptive immunity testifies to their important immunoregulatory role both in homeostasis and various types of pathology, particularly in the development of malignant tumors.

Morphological changes in cows’ mammary gland tissue under chronic form of mastitis

Nowadays, there are many different methods for diagnosing cow’ mammary gland diseases. This is a clinical examination of the general condition of the animal, organoleptic, physico-chemical, bacteriological research of the secretion of the mammary gland, radiological, ultrasound, laser, histological, cytological diagnostics, etc. Since all these methods in most cases are aimed at evaluating functional changes in the mammary gland and do not reflect the structural changes of the organ, so in the development of therapeutic and preventive measures to prevent the occurrence of pathological processes in the mammary gland in cows do not display information on pathomorphological changes in the tissues of mammary glands for mastitis of various forms. Histological diagnostics becomes an integral part of the clinical examination of animals in the activity of the doctor of veterinary medicine, because it allows taking into account the changes occurring in the tissues of the mammary gland, not only on the macroscopic, but also on the microscopic levels, which is especially important in differential diagnosis. Microscopic studies of biopsy revealed that normally the wall of the lacrimal alveolus consists of a single-layer cubic epithelium, a layer of myoepithelial (basintine) cells, a basement membrane and perialveolar connective tissue that contains numerous blood and lymphatic capillaries and nerve fibers. On the internal walls of the alveoli, there are secretory cells (lactoxytes) which, at different stages of their accumulation of secretion, or its separation into the lumen of the alveoli. The entire lumen of the alveoli is completely filled with a secret, which is rather intensely colored with eosin. In damaged alveoli, we detected the destruction of in situ lacticates, their excretion into the lumen with subsequent destruction there. In the destruction and removal of a small number of lacto-cysts in the lumen of the alveoli was a formed secret, in which an increased number of lactocytes and single neutrophils and lymphocytes were recorded. The given results indicate the pathohistological changes in cows’ mammary gland tissues under the chronic form of the mastitis. The method can be used as one of the important tests in the differential diagnosis of mastitis, which makes it possible to carry out in-depth study of the structure of the mammary gland of cows for various types of pathology using the method of biopsy of tissues from the affected area and the histological examination of the biopsy. Histology, as the most important scientific instrument of biology and medicine, is the only way to detect pathological changes in the tissues of the mammary gland. That is why histology of breast pathology serves as the most accurate diagnostic method in veterinary medicine and helps to choose the most effective way of treatment.