Various Kinds Of Tissues Research Articles

Tumour marking properties of different haematoporphyrins and tetrasulfonated phthalocyanine—A comparison

Several photosensitizers were screened for their tumour-marking ability using laserinduced fluorescence in Wistar/Furth rats bearing subcutaneous adenocarcinomas inoculated in muscle. Of the studied photosensitizers, dihaematoporphyrin ether appeared to exhibit the best tumour-demarcation properties. Polyhaematoporphyrin ester and tetrasulfonated phthalocyanine were almost as good although the fluorescence yield was much lower. Monomeric haematoporphyrin also showed some tumour-marking qualities. By forming fluorescence intensity ratios, information from both the blue and the red spectral regions were used to provide the highest tumour-to-muscle contrast. Two excitation wavelengths were used, of which 337 nm rather than 405 nm excitation light seemed to yield a better tumour demarcation, due to a greater difference in the superimposing autofluorescence between tumour and surrounding tissue. The study included measurements on many inner organs in an attempt to gain a better understanding of the interaction between the drugs and various kinds of tissue.

Histochemistry : IX. Distribution of saikosaponins in bupleurum falcatum root

The root of Bupleurum falcatum cultivated in Japan was examined by histological and chemical means in order to clarify the distribution of saikosaponins in the various kinds of tissues and parts of the root. By means of qualitative thin-layer chromatographic—densitometric and quantitative high-performance liquid chromatographic analyses, saikosaponins, bioactive principles in the root, were found to be abundant in the outer phloem layer, especially the pericycle and its neighbouring parenchyma cells in the root. It was also found that the highest level of saikosaponins was detected in thinner root hairs and that the level decreased towards the thicker root head.

牛の卵巣奇形腫の一例

Ovarian teratoma was found in the right ovary of a 2-year-old cow. The ovary was 16.0×15.0×11.0cm in size with a large follicular cyst containing 1, 250ml of follicullar fluid. The teratoma was very hard and 7.5×3.5×3.0cm in size. It had various kinds of tissues, such as cartilage, pulmonary, bronchial and gland-like tissue, epidermis, and hair, in irregular arrangement. The left ovary was normal.

Electron microscope studies on the intestine using ruthenium red.

Biopsy specimens of human intestines in various disease states and from laboratory animal intestines have been stained with ruthenium red and inspected by electron microscopy. Ruthenium red reaction product is found as a homogeneous deposit and also in the form of coarse and fine granules. Microvilli and the glycocalyx are stained relatively constant by ruthenium red. The intercellular space of the epithelium shows a strikingly variable ruthenium red staining. This variation is apparently not related to the method but indicates differences or variations in the functional state of the membranes. It remains an open question whether the ruthenium red reaction is a histochemical reaction, marking certain substances present in in the intercellular area or whether it should be considered as a tracer for the transport of materials. Goblet cells and necrobiotic cells usually show a constant ruthenium red staining. In the lamina propria the reaction product is very inconstant, possibly on account of the long penetration path from the lumen. The phagolysosomes of macrophages are marked by ruthenium red rather regularly. No fundamental, but only quantitative differences have been found between the various kinds of tissue studied.

アコヤガイの物質代謝に関する研究-III

Among various kinds of tissue, the rate of respiration was the highest in the gills and very low in such tissues as the adductor muscle and outer epithelium adhering to the inner surface of shell (Table 1). The amount of oxygen consumption of the gills, measured at 25°C., was higher in winter than in summer (Table 2). The respiration of excised gill piece was blocked by cyanide, azide, αα'-dipyridil, salicylaldoxime and iodoacetate, and stimulated by methylene blue and 2, 4-dinitrophenol (Table 3, Fig. 1 & 2). The inhibition by salicylaldoxime seems to suggest that a copper enzyme might play an important role in the respiratory system of the gills as well as an iron enzyme, cytochrome oxidase, which has been previously proven by the writer.

泉熱ウイルスの組織培養及び孵化鶏卵内培養について

Cultivation of Izumi-fever virus in tissue culture was attempted with various kinds of tissue, i.e., heart, lung and kidney of suckling mouse, rat, guinea pig, sand rat and human embryo, and chicke mbryonic skinmuscle and chorioallantoic membrane.The cultivation of this virus was carried out through stational method in the medium consisting of Hanks' solution 60%, ultrafiltrate horse serum 30% and chick embryo extract 10%.No cytopathogenic effects were observed in any case. Therefore, histopathological changes of rats sacrificed 30 days after inoculation with the culture fluid of the 3rd passage of tissue culture were investigated. The histopathological changes showed that this virus multiplied more favorably in heart and lung tissue than in kidney tissue throughout all species of animal used.In developing hen's egg, yolk sac method and allantoic cavity method were used. In both cases, this virus failed to cause fetal infection, and no hemagglutination was observed. The histopathological changes of rats inoculated with egg material of serial passages, showed that yolk sac method was superior to allantoic cavity method to cultivate this virus.