The present study investigated callusing frequency of explants from leaf and stem tissues obtained from Capsicum annuum explant exposed to waste engine oil contamination. The explants obtained from 21-31 days old seedlings were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of BAP (0, 1, 2, 3, 4, 5 and 6 mg/l) and NAA, IAA, 2, 4-D and IBA (0, 5, 10, 15 and 20 mg/l). Results showed that efficient callusing in leaves and stems explants (21 days old) was achieved after explants were cultured on MS semi-solid media containing 1-5 mg/l BAP and 0.5-1.5 mg/l IBA for 4 weeks; while callus formation was hindered in older tissues. An effective protocol for callogenesis of 21 day-old explants (stems) of C. annuum obtained from oil-polluted soil in a semi-solid state MS medium using BAP and IBA was thus developed. The study also documented the ability of IBA to induce callus.