Various Concentrations Of BAP Research Articles

Micropropagation of Lepidium ostleri (Brassicaceae), a native endemic plant species

Lepidium ostleri S.L. Welsh & Goodrich (Ostler’s peppergrass) is an endemic plant species restricted to Ordovician limestone outcrops associated with the San Francisco Mountain Range in western Utah. Due to restricted population distribution and proximity to modern mining operations, L. ostleri is a species of conservation interest. This study focused on the development of a micropropagation protocol for propagating mature plants using plant tissue culture methods. Indirect shoot organogenesis was obtained from L. ostleri explants on Murashige and Skoog (MS) medium augmented with various concentrations of BAP (6-Benzylaminopurine), kinetin (N6-furfuryladenine), and IAA (indole-3-acetic acid). Plantlets supporting shoots grown in vitro were pulse treated with differing strengths of indole-3-butyric acid (IBA) and transferred to sterile soil. Following root induction, plantlets were acclimated to ambient conditions. The successful development of a micropropagation protocol supports management activities for L. ostleri and also contributes to in vitro propagation knowledge at the species, genus, and family levels.

Micropropagation of strawberry (Fragaria ananassa “pajaro”)by meristem culture method

Experiments were carried out to examine the effects of different combinations of plant growth regulators in vitro micropropagation of strawberry plants. Specimens were sterilised by a different concentration of sodium hypochlorite - NaOCl (1, 2, and 3%) for 20 mins. A meristem was separated and cultured on Murashige and Skoog (MS) containing 0.3 mg/l BAP for 8 weeks. Initial shoots were transferred to MS with various concentrations of BAP (0, 0.1, 0.3, and 0.5 mg/l) and kinetin (0, 0.1, 0.2, and 0.3 mg/l) in either single or combination. After 6 weeks of culture, the regenerated shoots were transferred on 1/2 MS with different concentrations of NAA (0, 0.1, 0.3, 0.5, 0.7, and 1.0 mg/l) and BAP (0, 0.1, 0.3, and 0.5 mg/l) in either single or combination. The results showed that the lowest percentage of contamination (10%) and the highest percentage of regeneration (80%) response were achieved on MS media by sterilisation with 3% NaOCl for 20 mins. The highest shoot proliferation per explant (16.2 shoots/explant) was reached on MS with 0.3 mg/l BAP plus 0.1 mg/l kinetin. 1/2 MS media with 0.1 mg/l NAA plus 0.1 mg/l BAP induced 14.4 roots per shoot and produced the longest roots (9.1 cm). However, the higher concentration of NAA (0.3-1.0 mg/l) or in combination with BAP resulted in callus formation. The plantlets, thus developed, were hardened and successfully established in soil containing organic fertiliser: Trichoderma: coconut peat (1:1:1 v/v/v).

In vitro Propagation and Assessment of Genetic Homogeneity using RAPD and ISSR Markers in Tinospora cordifolia (Wild.) Hook. F. & Thoms, An Important Medicinal Plant of Nepal

The Menispermaceae family includes the widely glabrous, succulent, climbing shrub Tinospora cordifolia (Gurjo), which has been found to have a variety of pharmacological and ethnomedicinal characteristics. T. cordifolia is also one of the most commercially exploited plants in pharmaceuticals. The nodal segment explants were initially cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzyl amino purine (BAP), and/or Kinetin (KIN) among which MS +BAP (2.0 mg/l) induced shoot initiation after 7 days of post-inoculation. The nodal segments were then excised and treated with various concentrations of BAP, BAP with KIN, and coconut water for the proliferation among which 5 mg/l induced significant nodal segment proliferation (9.0 nodal segments/per explant) and shoot length (8.0 cm). The efficacy of coconut water in increasing the nodal segment proliferation of T. cordifolia was tested and the shoot proliferation increased significantly at 5% and 10% of coconut water, however, the maximum response of shoot number (23.0), shoot length (12 cm) was in the MS medium supplementation with BAP (5mg/l) and 10% coconut water. The genetic fidelity of these plants was also confirmed by random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers in wild and in vitro cultures. This protocol is an efficient way for the in vitro mass propagation of true-to-type plantlets of T. cordifolia which provides a basis for germplasm conservation and sustainable utilization.

In vitro Micropropagation of Berberis chitria (Lindl.) - A Rare Medicinal Plant from Himachal Pradesh, India

An efficient in-vitro micropropagation protocol has been developed for Berberis chitria (Lindl.) - a rare medicinal plant from Himachal Pradesh, India using seeds and in-vitro nodal explants. The effects of different concentrations of BAP and GA3 for in-vitro seed germination was investigated and consequently 6.0 mg/l BAP + 0.0 mg/l GA3 was reported to be excellent for seed germination. Callus induction from leaf explants from aseptically grown seedling were also studied by using various concentrations of BAP and 2, 4-D and showing 94.26 ± 0.12% response at the concentration of 2.0 mg/l BAP + 0.4 mg/l 2, 4-D. The highest percentage of multiple shoot induction was obtained (100.00 ± 0.01% with an average of 4.50 ± 0.3 shoots) on MS medium augmented with 6.0 mg/l BAP + 1.0 mg/l Kn + 1.0 mg/l IAA. Whereas, the combined concentration of 1.0 mg/l NAA + 1.0 mg/l IBA + 0.5 mg/l IAA showed highest (76.05 ± 0.09%) root induction with an average of 3.84 ± 0.27 roots per shoot. The survival rate of these plantlets under greenhouse condition was 85%. This research communication provides first record about the in-vitro multiplication of B. chitria, collected from Himachal Pradesh Himalayas of India. Plant Tissue Cult. & Biotech. 32(2): 227-236, 2022 (December)

MICROPROPAGATION OF ONION (ALLIUM CEPA L.) THROUGH BULBLET FORMATION

Multiple shoot clumps or microclones were collected from two onion (Allium cepa L.) varieties for bulblet formation. MS medium supplemented with various concentrations of BAP, Kn, 2ip and NAA were used. The height responded shoot was observed 90% and 80% in Taherpuri and Indian variety, respectively on MS supplemented with 2.0 mg l-1 BAP+0.5 mg l-1 NAA. The best bulblet formation was obtained in MS containing 1.0 mg l-1 2ip. Finally, the rooted bulblets were hardened and established in soil under natural environment.

Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

Dutch iris is a commercially important bulbous ornamental crop. Its high demand in global floriculture market necessitates the production of its high-quality planting material. In the present investigation, an efficient in vitro propagation system has been developed for Iris × hollandica Tub. cv. Professor Blaauw (Dutch iris) using meta-Topolin (mT) for the first time. Effect of various concentrations of BAP, Kn, and mT (0, 0.5, 1.0, 1.5 and 2 mg L−1) along with varying photoperiods (16 h light and dark incubation for 1, 2, 3 and 4 weeks) on in vitro shoot induction from the twin scale explants was studied. Of the cytokinins tested, different doses of mT has resulted in better shoot induction response from twin scale explants than BAP and Kn. Photoperiod duration has also affected shoot induction response significantly. Along with dark incubation for 1 week, cytokinin mT at 1.0 mg L−1 in MS medium has resulted in maximum shoot induction response (91.63%) with increased emergence of micro shoots (4.83 shoots/explant with average shoot length of 5.02 cm). Efficacy of BAP and mT alone or in combination with auxins for in vitro shoot multiplication was also compared. The synergistic effect of cytokinin-auxin in multiplication medium comprising MS + 1.0 mg L−1 mT + 0.25 mg L−1 NAA resulted in considerably higher number of shoots (17.53) with mean shoot length (7.06 cm) and maximum number of bulblets (2.74). Positive effect of increased sucrose concentration (90 g L−1) alone or with paclobutrazol (5 mg L−1) on in vitro bulblet formation and bulblet size was observed respectively. The superiority of mT over BAP was also found during in vitro rhizogenesis. Shoots raised on the mT medium were healthy and long enough, thus showed better rooting response (63.83%) on ½ MS medium + 0.5 mg L−1 IAA after 4 weeks of incubation. About 89.16% survival rate was recorded for in vitro raised plantlets under ex vitro conditions. Analysis of clonal fidelity of thirteen in vitro regenerated plants was done using SCoT markers. Out of 36 primers, 13 primers showed clearly scorable monomorphic bands, thus displaying genetic uniformity among in vitro regenerated plantlets. This mT mediated protocol can be routinely used for the rapid large scale production of this valuable floriculture crop.

Stimulation effect of synthetic plant growth regulator (GA3 and BAP) on young cinchona plant (Cinchona ledgeriana) grown in lowland

Cinchona is a pharmaceutical plant that produces medical substance. This plant contains copious type of alkaloid such as quinine, quinidine, cinchonine, and cinchonidine. Several obstacles might restrict cinchona cultivation, mainly climatic factors such as temperature and rainfall. One of the efforts to improve cinchona growth in lowland through the application of synthetic plant growth regulator (PGR). Two stages of experimental design were used in this study: first stage was the application of GA3 in six levels from October to December 2017 and the second stage was six levels of BAP concentrations treatment from July until October 2018. The experimental design used was randomized block design (RBD) with 4 replications at low altitude area for cinchona plant. The results showed GA3 applications have a significant effect towards leaves width and plant height. The 225 ppm of GA3 dosage produced maximum leaf width, 100ppm yielded the maximum number in plant height, and 150ppm produced the highest leaf count. While GA3 as a PGR improved the vegetative characters, various concentration of BAP had positive impact for stem diameter, leaves number, leaves area, and chlorophyll content on young cinchona plant grown in lowland area.

In vitro seed germination and elicitation of phenolics and flavonoids in in vitro germinated Trigonella foenum graecum plantlets

Abstract Trigonella foenum graecum(fenugreek) is an important leguminous plant which exhibits numerous pharmacological properties due to the presence of many important plant secondary metabolites. This study reports the effects of BAP and NAA on in vitro seed germination of Trigonella foenum graecum and the effect of salicylic acid elicitor on total phenolic and flavonoid content and ultimately antioxidant activity of in vitro grown plantlets. Seeds were cultured on MS media with various concentration of BAP and NAA (0–2 mg/L) added singly and in combinations. Germination percentage, shoot length, number of leaves and root length was recorded weekly for 5 weeks, and a final mean of each parameter was taken. Based on the results 2.0 mg/L BAP produced the highest response in all parameters (germination: 93.33 ± 3.18%, shoot length: 3.26 ± 0.08 cm, number of leaves: 13.73 ± 0.18 and root length: 1.12 ± 0.02 cm). After 5 weeks, plantlets were subcultured on MS media supplemented with BAP 2.0 mg/L treated with various concentrations of salicylic acid (100–400 mM) for 48 h. The control did not contain any elicitor. The plantlets were then oven-dried and extracted using methanol by rotary evaporation and tested for antioxidant activity, phenolics and flavonoids. Salicylic acid concentration of 100 Mm produced the highest response in antioxidative activity (89.84 ± 0.10%) and yielded the highest TPC (16.55 ± 0.21 mg GAE/g extract) and TFC (58.89 ± 4.29 mg QE/g extract) among other elicitor concentrations. Trigonella foenumgraecum plantlets exhibited remarkable antioxidant capacity upon elicitation with lower concentrations of salicylic acid. Hence, it is concluded that low concentrations of salicylic acid could effectively be used as potent elicitor to enhance the synthesis of phenolic and flavonoid compounds in vitro fenugreek plantlets.

Evaluation of different growth hormones mediated callus induction and regeneration as well as the effect of colchicines, ethyl methanesulfonate (EMS) and gamma radiation on some traits of Impatiens walleriana

Impatiens is an ornamental member of family Balsaminaceae. This plant mostly propagated by vegetative technique, which generally time wasting process. It is often multiplicities via seed but is barricaded by F1 seed sterility. In vitro culture of Impatiens walleriana has much significant function in fast proliferation with useful features and elicitation of healthful and disease-free plants. This experiment was conducted to investigate the impact of medium and different hormones on in vitro propagation of Impatiens walleriana by using a completely randomized design. MS medium was prepared along with various concentrations of BAP, TDZ and ZEA. Callus was induced and grew well in media supplemented with 0.5 mg/l NAA + 1 mg/l BAP. In order to indirect propagation, explants were cultured in same media containing BAP, ZEA and TDZ in combination with NAA. These treatments have ability to organogenesis. The results revealed that the control treatment had the lowest effect on traits including shoot percentage, number of shoots, number of leaves, shoot length, fresh and dry weight, and it lead to maximum proliferations in medium supplemented with 0.5 mg/l NAA + 1 mg/l BAP. The highest root length and rooting percentage was observed in 0.5 mg/l IBA + 0.5 mg/l BAP. In addition, the effect of mutation agents was studied. Aseptic samples were treated with ?- irradiation, Ethyl Methane Sulfonate and colchicines at growth chamber. Treatments with 30 and 60 grey respectively had the lower survival rate, growth rate and polyploidy while colchicines with 0.1 and 0.2 had the highest rats. Regarding to these, the present technique illustrate an effective system for in vitro reproduction of Impatiens walleriana by hypocotyls cultures. In addition, colchicines proved to be effective in induction of polyploidy in this plantlet.

In vitro propagation of white mahang (Macaranga hypoleuca (Reichb.f.et Zoll.) Mull Arg.)

White mahang (Macaranga hypoleuca Mull.Arg) is considered as an alternative species for light construction and traditional medicine. In vitro propagation, it has been attempted through series of in vitro propagation protocols, i.e., explant sterilization, shoots multiplication, root induction, and acclimatization using terminal and axillary buds explants. WPM basal medium supplemented by plant growth regulators BAP (cytokinin group), NAA and IBA (auxin group) were used for shoot regeneration studies. Various concentration of BAP (0, 0.5, 1 mg l−1), IBA (0, 0.1, 0.5, 1 mg l−1) and NAA (0, 0.01, 0.05, 0.1 m l−1) were tested for shoot induction and elongation. Interaction between BAP concentration and explant types, both those taken from apex- and axillary- buds showed significant differences for shoot induction and elongation. The highest number of shoots were resulted from the WPM media containing BAP (0.5 mg l−1), while the addition of IBA (0.5-1.0 mg l−1) and NAA (0.01-0.1 mg l−1) combined with BAP (0-1.0 mg l−1) produced a high percentage of callus (> 60%). In vitro, rooting was induced on half-strength MS media supplemented with IBA (1 mg l−1). The regenerated shoots with developed root systems were successfully acclimatized and established in pots containing sand and compost (3:1).

MICROPROPAGATION OF AN ENDANGERED AND ENDEMIC MEDICINAL PLANT CAYRATIA PEDATA VAR. GLABRA

Objective: The objective of the present study was to develop standardization protocol for the successful in vitro mass propagation of Cayratia pedata var. glabra through leaf and stem explants, since it is a rare, endangered, and endemic medicinal plant using biotechnological involvements and to conserve this endangered species.
 Methods: The application of biotechnological principles for the establishment of micropropagation under in vitro conditions has been studied by following the methods. The explants, namely, leaf and stem harvested from in vivo plants were thoroughly washed and properly sterilized with sterilients. The explants were transferred to Murashige and Skoog (MS) medium supplemented with growth regulators 6-benzyladenine (BAP) and naphthalene acetic acid (NAA) in the concentration range of 0.5–3.0 mg/l which were tested for callus induction and morphogenesis. The elongated shoots were transferred to MS medium supplemented with NAA at different concentrations for root induction.
 Results: The explants collected from the field (shola) were treated in different steriliants with various concentrations at different time for sterilization. Among the various combinations tried, the Teepol treatment for 10 min followed by bavistin 20 min, antibiotics, namely, ampicillin and rifampicin for 20 min, 70% alcohol for 30 s, and 0.12 % HgCl2 for 3 min was found to be effective. The explants were cultured in MS medium supplemented with various concentrations of BAP and NAA. The results noted that an increase in the concentration of BAP concomitantly reduced the frequency of callus formation. The maximum callusing frequency and more number of shoot formation was observed in the lower concentration of BAP (0.5 mg/l) in combination with NAA (0.2 mg/l). The callus obtained from all the above combinations was sub-cultured on MS medium with same combinations of BAP and NAA. The maximum frequency of root formation in leaf callus was 85% and 75% in stem callus and both were achieved on MS medium with NAA (1 mg/l) after 2 weeks.
 Conclusions: The current investigation provides a competent in vitro propagation method for C. pedata var. glabra which could be commercialized for developing identical plants with high-quality mass multiplication rate and for better conservation of the germplasm. Both the methods described here are well suited for the mass multiplication of this critically endangered and endemic climber species.

Rapid propagation of Celastrus paniculatus Willd.: an endangered medicinal plant through indirect organogenesis

A rapid and efficient propagation system through callus explant derived from leaf was established in Celastrus paniculatus Willd., a medicinal plant of the Celastraceae family. Seed dormancy and vegetative propagation render it for developing an in vitro regeneration method. Murashige and Skoog (MS) media containing 6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthalene acetic acid (NAA) and with various concentrations of BAP + NAA, BA + 2,4-D and BAP + IAA produced different natures of calli. Moreover, BAP + NAA produced friable callus, whereas BAP + 2, 4-D produced compact calli, which were transferred to the shoot initiation medium containing BAP supplemented with ascorbic acid and each of adenine sulfate, arginine and citric acid. Inclusion of meta-topolin in the media along with optimum concentration of BAP promoted shoot multiplication and elongation after 8 weeks of culture. The in vitro elongated shoots were treated with different auxins such as IAA (Indole 3 Acetic Acid), IBA (Indole-3-butyric acid) and NAA (Naphthalene acetic acid) individually for early rooting and the treated shoots were transferred to the half-strength MS medium. The regenerated plantlets were acclimatized in pots containing sterilized soil and sand and then transferred to the field conditions, 90% of the regenerants survived. Thus, this was the first report on indirect organogenesis of C paniculatus Willd. using callus explant obtained from direct organogenesis leaf.

In vitro high frequency multiplication and assessment of genetic fidelity of Corallocarpus epigaeus: an endangered medicinal plant

Corallocarpus epigaeus (Rottler) Hook.f. is an endangered tuberous medicinal climber of family Cucurbitaceae. Despite high medicinal value, over-exploitation made it threatened. In vitro propagation has been adopted for conserving this endangered medicinal plant. Direct shoots induction was achieved from nodal explants on MS medium fortified with various concentrations of BAP and TDZ individually and BAP + IAA, TDZ + IAA, BAP + l-glutamic acid and TDZ + l-glutamic acid combinations. The highest frequency of multiple shoots (43.33 ± 0.53) was achieved on MS medium fortified with 1.5 mg/l TDZ + 1.5 mg/l IAA from nodal explants but shoot length (12.9 ± 0.15 cm) was high on MS medium supplemented with 1.0 mg/l TDZ and 2.0 mg/l l-Glutamic acid. The highest percentage (78%) of rooting was achieved on half strength MS medium augmented with 1.0 mg/l IBA with a mean number of roots 10.76 ± 0.30 cm, an average root length is 1.69 ± 0.07 cm. Rooted plantlets were acclimatized in the greenhouse and successfully transplanted to natural conditions with a 68% survival rate. ISSR markers were used to check the genetic fidelity between in vivo and in vitro developed plantlets. The results indicated that the micropropagated plants are monomorphic and true type when compare with mother plant.

The Formation of Shoots in Mangosteen (Garcinia Mangostana L.) Nodules From Bengkalis, Riau in Murashige Skoog and Woody Plant Medium

One of the mangosteen producers in Indonesia is Riau Province, Bengkalis regency to be exact. This mangosteen has some superiority, such as able to grow in peat swamp1). Nodules are cells that develop to become shoots only in proper nutrients and conditions. This study aimed to discover the response of mangosteen nodules (Garcinia mangostana L.) on MS and WPM media by adding BAP. This study used Completely Randomized Design using various concentrations of BAP and different media. The results of this study indicate that the best response on MS medium is the percentage of nodule formation at 1 mg / l BAP of 49.99%. The fastest callus appears on control and 5 mg/l BAP 17.25 and 6.75 days after planting (dap). The most significant number of nodules at 1 mg/l of BAP is 1.50 nodules. The callus texture is compact and crumbs with white and brown colour. The best response on WPM media is the percentage of formation of a nodule at 1 mg/l of BAP resulting 33.33%. The fastest shoots emerge at 1 mg / l BAP 13.25 dap. The highest number of nodules at 1 mg/l of BAP is 1.00 nodule, the highest leaf amount at 3 mg/l BAP was 6.75 sheets, and the highest shoot length at 5 mg / l BAP is 1.32 cm. The texture callus compact and crumb with white colour.

Investigating the effect of antioxidant compounds and various concentrations of BAP and NAA on the improvement of in vitro stem and root formation of Nepeta binaloudensis Jamzad

Investigating the effect of antioxidant compounds and various concentrations of BAP and NAA on the improvement of in vitro stem and root formation of Nepeta binaloudensis Jamzad

Impact of Explants, Plant Growth Regulators and their Interaction on Micropropagation of Impatiens balsamina, L.

The aim of this study is to improve the propagation of Impatiens balsamina, L by an efficient protocol for in vitro propagation was successfully established through tissue culture technique. Cotyledonary node with sections of cotyledon, section of cotyledons and hypocotyls were derived from 21 days in vitro germinating seeds after surface sterilization. They were cultured for shoot multiplication and callus induction on MS medium fortified with various concentrations of BAP, TDZ or combination between BAP and NAA. It was determined that cotyledonary node with sections of cotyledon on MS medium supplemented with 4.5 mg/L BAP for maximum shoots number (4.33shoots) and callus induction 3.78. The best shoot length (3.08 cm) within 8 weeks from regeneration was achieved on MS medium fortified with 1.0 mg/L BAP + 0.5 mg/L NAA. The number of shoots formed per explants increased by re-culturing for six weeks on the same medium (4.5 mg/L BAP alone), since it produced (9 shoots). While the best shoot length (4cm) was obtained by sub culturing the explants on MS medium received 1.0 mg/L BAP + 0.5 mg/L NAA to MS without growth regulators after six weeks. The best callus fresh weight (9.32 g) and callus volume (7.76 cm3) were recorded with using TDZ at 3.0 mg/L that sub cultured on medium fortified with 4.5 mg/L BAP. In addition the highest dry weight (0.81 g) was obtained by re - culturing on the same medium that contained with 4.5 mg/L BAP.

Effects of Cultivation Media on In vitro Callus Induction and Regeneration Capabilities of Pakaumpuel Rice (Oryza sativa L.), Thai Rice Landrace

Current study determined the effects of different cultivation media and some factors on in vitro callus induction and shoot regeneration of Pakaumpuel rice (Oryza sativa L.), the Thai rice landrace. Pakaumpuel’s dehusked seeds were surface sterilized by 20% Sodium hypoclorite for 20 mins before washed and cultured on Murashige and Skoog (MS) medium in various concentrations of BAP (0, 0.1, 0.5 and 1 mg/l) and 2,4-D (0, 1, 2 and 3 mg/l) and cultivated in off -light or dark cycle condition for 15 days. Results showed that MS medium with 2 mg/l 2,4-D incubated in light condition provided the highest callus induction percentage (80 %). For shoot regeneration, calli were desiccated by using silica gel for 0, 30, 90 and 180 minutes prior to culture on MS medium with 1 mg/l NAA and various concentrations of BAP (0, 1, 2, 3 and 4 mg/l) for 30 days. The results revealed that desiccation periods had no effect on regeneration percentage and number of shoot per callus, but had effects on shoot length and number of leaves per shoot. Therefore, MS medium with 1 mg/l NAA and 3 mg/l BAP induced the highest regeneration percentage (53.33 %) and number of shoot per callus (4.40 shoots), while the longest shoot (12.82 cm) and the highest number of leaves per shoot (3.00 leaves) were found on the culture on this medium after desiccation for 90 minutes. This discovery can be applied for micropropagation of other Thai rice landraces.

EFEITO DA CITOCININA 6-BENZILAMINOPURINA (BAP) SOBRE O ESTABELECIMENTO IN VITRO DE SEGMENTOS NODAIS DE Rosa sp.

RESUMO: As rosas são consideradas uma das plantas ornamentais mais importantes do mercado floricultor. Tendo isto em vista, empregar novas tecnologias produtivas para esta cultura é de grande importância científica e econômica. Por isto, o objetivo neste trabalho foi avaliar o efeito de diferentes concentrações da citocinina 6-benzilaminopurina (BAP) sobre a indução de brotos em Rosa sp. in vitro. Segmentos nodais contendo gemas axilares de uma matriz comercial de Rosa sp. foram suplementados com diversas concentrações de BAP (0,0; 0,25; 0,5; 1,0; 1,5 mg/L). As maiores taxas de explantes responsivos (que apresentaram brotações) foram obtidas nas maiores concentrações do hormônio BAP, embora não haja diferença estatística significativa a nível de 5% entre os tratamentos. Portanto a variação da concentração de BAP influenciou no número de explantes estabelecidos in vitro, proporcionando maior sobrevivência e brotações nos segmentos nodais de Rosa sp.PALAVRAS-CHAVE: Cultura de tecidos, Micropropagação, Rosa.

Pengaruh konsentrasi IAA (Indole Acetic Acid) dan BAP (Benzil Amino Purin) terhadap pertumbuhan awal sambung samping Jambu Biji (Psidium guajava) Var. Kristal

The purpose of this research was to study the influence of various concentrations of IAA and BAP on early growth budding of guava (Psidium guajava L.) Var. Kristal in side grafting propagation method. The experiment used completely randomized factorial design. The first factor was various concentrations of IAA (0, 100, and 200 ppm) and the second factor was various concentrations of BAP (0, 100, and 200 ppm). Each treatment was repeated three times. Parameters observed were the time of bud break, time of shoot emergence, and shoot length. The data were analyzed by variance and continued analyzed by Duncan Multiple Range Test. The results showed that the level of IAA concentration gave effect (P<0,05) to time of shoot emergence and shoot length. Level of BAP concentrations gave significant effect (P<0,05) to the time of bud break, time of shoot emergence, and shoot length. The interaction between the concentration level of IAA and BAP had significant effect (P<0,05) on the time of bud break, time of shoot emergence and shoot length. Keywords : Guava, Psidium guajava, Side Grafting, IAA, BAP.

Effect of growth regulators on in vitro multiplication of potato (Solanum tuberosum L.) cv. diamant

Shoot tip and nodal segment explants from field grown plants were used as experimental materials in this investigation. All explants were cultured on MS medium supplemented with various plant growth regulators. For surface sterilization of explants, HgCl2 (0.1%) for 2 minutes was found to be most effective for complete killing of surface pathogens and getting healthy tissues. Shoot regeneration was observed from both shoot tips and nodal explants for the studied plant. Various concentrations of BAP (0.1, 0.2, 0.3mg/l) and GA3 (0.1, 0.2, 0.3mg/l) were used for shoot multiplication. In case of BAP, the highest length of shoot was recorded 4 cm and the highest percentage of shoot multiplication (73%) was noticed in MS+0.2mg/l BAP. And in case of GA3, the highest response for shoot multiplication (82%) was noticed in MS+ 0.1 mg/l GA3.But among all of the media formulations used in this experiment, the highest response for shoot multiplication (95%) within7-10 days was noticed in hormone free MS media. In case of root regeneration, the highest percentage (96%) of root induction was recorded in MS medium supplemented with no hormone. Asian Australas. J. Biosci. Biotechnol. 2017, 2 (1), 81-88