Sanitary monitoring of spring wheat agrocenosis in the zone of unstable moisture was carried out to determine the degree of development and prevalence of diseases. The fungicidal activity and effectiveness of seed protectants of various classes against seed infection on spring wheat variety Yasenka have been studied. The results of the research established the effectiveness of the disinfectant DVD Chance, KS, corresponding to 85.7% relative to the effectiveness of the standard Polaris, ME Eco Plus (100 g/l prochloraz + 25 g/l imazalil + 15 g/l tebuconazole) (84.6%). An increase of 15–20% in damage to spring wheat plants by root and basal rot caused by fungi of the genus Fusarium in the “tillering” phase and its dependence on the climatic conditions of the growing season was established.By the “flag leaf” phase, the prevalence of root and basal rot caused by fungi of the genus Fusarium increased to 20,5%, and the degree of development — to 7–9%.Evaluation of the effect of the fungicide DVD Chance, KS (30 g/l difenoconazole + 6.3 g/l cyproconazole) by pre-sowing treatment of spring wheat grain of the Yasenka variety established the effectiveness of the drug at a consumption rate of 1.0 l/t against molding of seeds, corresponding to 85.6% , in the reference variant the indicator is 84.6%, with the development of the disease in the control — 34.5%. By harvest, the effectiveness of the drug remained and reached 79.2%, in the standard — 78.3% with the development of the disease 43.7%. Research contributes to the expansion of the range of fungicides for treating grain crops, the development of regulations for use and the possibility of recommending them for registration tests for further inclusion in the State Catalog of those approved for use.