Bean Varieties Research Articles

Pengaruh Dosis Pupuk Kandang Kambing terhadap Pertumbuhan dan Hasil pada Dua Varietas Kacang Panjang (Vigna unguiculata L.)

Poor choice of varieties and the decrease in soil fertility are among the few variables that can affect long peanut productivity. The aim of this research is to study how the dose of goat fertilizer and long bean varieties affects the growth and yield of long beans. The research was a factorial experiment with two factors and three replications using the Randomized Blok Design (RBD) method. The first factor, the dose of goat manure 0 ton ha-1(k0), 10 ton ha-1(k1), 20 ton ha-1(k2), 30 ton ha-1(k3), and 40 ton ha-1(k4). The second factor was long bean varieties Kanton Tavi (v1) and Katrina (v2). Data were analyzed using analysis of variance (ANOVA) at the 5% level. The results of the experiment showed an interaction in the parameters of plant height 7 DAP and 14 DAP, number of leaves 14 DAP, 21 DAP, 28 DAP, number of pods per plant, and pod weight per plot. A dose of 30-ton ha-1 (k3) goat pen fertilizer produced a healthy amount of beans on the cantonese Kanton Tavi variety (v1) with X optimum (v1) = 29,08 ton ha-1 and Y maximum (v1) = 21,86 ton ha-1 and on Katrina variety (v2) X optimum (v2) = 25,15 ton ha-1 and maximum y (v2) = 20,56 ton ha-1.

Optimizing faba bean cultivation: assessing varietal performance in spring and fall

The need to identify specialty crops in Virginia has driven interest in faba beans (Vicia faba L.), which offer potential benefits for crop rotation systems. As a cool-season crop, faba beans can be planted in both fall and spring, providing flexibility in farming schedules. A field study was conducted at Randolph Farm, the Virginia State University Research and Extension Farm, using a completely randomized factorial block design. This study examines the performance of seven faba bean varieties—Ethiopia, NEB247, Aprovecho, EN3, EN47, Windsor and EN45—across three spring (late February, late March and mid April), and three fall (late September, early October and late October) planting dates. Our results demonstrate that both variety and planting date significantly influence the yield and yield components of faba beans. Among the varieties tested, Windsor and EN47 exhibited superior traits across multiple categories, making them preferable for achieving high yields. Conversely, varieties such as EN45, Aprovecho, and NEB247 showed poor performance. Fall planting dates generally resulted in superior growth, yield, and maturity characteristics, underscoring their importance for maximizing faba bean production. We observed that faba beans planted in the fall had 58% more branches, 100% more shoot dry matter, 34% higher 100-seed weight, double the grain yields, and 8% higher harvest index compared to those planted in the spring. To further enhance faba bean production, additional studies are suggested to clarify the physiological relationships between photosynthesis rates and the sink-source dynamics. Furthermore, investigating how planting dates impact the nutrient components of faba beans will provide deeper insights into optimizing their cultivation.

A COMPREHENSIVE REVIEW OF RECENT ADVANCEMENTS IN VIGNA RADIATA RESEARCH: INSIGHTS INTO PHYSIOLOGY, AGRONOMIC, NUTRITIONAL, AND HEALTH PERSPECTIVES

Aims: The primary aim of this research is to provide a better comparative understanding of the Physiology, Agronomic, Nutritional, and Health-modulating effects observed in the reviewed studies related to Vigna radiata consumption. The review paper typically highlights the antidiabetic, anticancer, anti-inflammatory, and immunomodulatory effects of Vigna radiata (mung bean). The fact that there exist inconsistencies and gaps in evidence against certain effects of mung bean & places where further research is needed is also addressed in the review paperPlace and Duration of Study: Department of Biotechnology, Techno India University, West Bengal, India; between August 2023 and July 2024.Methodology: The paper has been prepared by conducting a comprehensive search of various mung bean-related scientific literature databases across the internet mung beans, followed by extracting relevant data from selected studies, evaluating quality assessment factors & finally summarizing and synthesizing the extracted data, including the main findings related to Vigna radiata's beneficial effects.Results: The review work seems to be beneficial for reaffirming the significance of Vigna radiata as a natural source of immunity support and summarizing the key takeaways from the review.Conclusion: The mung bean (Vigna radiata) is a leguminous fodder crop which is rich in nutrients and bioactive compounds, including polyphenols, polysaccharides, and polypeptides. Studies conducted in vitro and in vivo highlight the variety of mung bean's health benefits. It portrays antidiabetic, anticancer, anti-inflammatory, and immunomodulatory effects. However, it is imperative to delve deeper into the mechanisms underlying their role in preventing diseases. There are substantial knowledge gaps regarding the bioactive compounds and biological functions of mung beans. Further investigation is crucial to pinpoint additional essential components responsible for their health advantages and underscore these various components' combined effects on biological processes.

Exploring common bean's defense arsenal: Genome-wide characterization of PR-1 gene family and its transcriptional response to Colletotrichum lindemuthianum inoculation

Pathogenesis-related Protein 1 (PR-1) plays a crucial role in plant defense responses, particularly against fungal pathogens. Despite its significance, comprehensive studies characterizing this gene family in the common bean (Phaseolus vulgaris) are currently lacking. Therefore, the objective of this study was to conduct genomic mining and characterization of the PR-1 in common bean (PvPR-1) genome. Additionally, we assessed the transcriptional expression of all its isoforms in response to inoculation with the fungus Colletotrichum lindemuthianum. This evaluation was performed on leaf tissue samples obtained from both sensitive (Rosinha) and resistant (Africano 4) common bean varieties at 24-, 48-, and 96-hours post inoculation. Thirteen PvPR-1 genes were consistently identified, forming two major clusters across the clustering analyses. Physicochemical characterization indicated that the PvPR-1 proteins are predominantly basic, hydrophilic, and extracellularly localized. Moreover, their promoter regions contain putative cis-regulatory elements that respond to a broad spectrum of plant hormones, including jasmonic acid, gibberellin, and ethylene, which are key regulators of both biotic and abiotic stress responses. This discovery implies a multifaceted role for the studied proteins in common bean physiology. KEGG pathway analysis implicated PvPR-1 proteins in hormonal signaling (corroborating the anchored cis-regulatory elements) and plant-pathogen interaction networks. Secondary structure evaluation revealed the predominance of α-helices and coiled structures within these proteins. Subsequent 3D modeling demonstrated a conserved ‘α-β-α’ sandwich architecture characterized by a central cavity. This structural motif suggests potential functional versatility, particularly in pathogen recognition and responses. Additionally, the study provided insight into the potential interactions of PvPR-1 with Chitinase II (PR-3) and Rab-18, as suggested by the STRING platform. Temporal differences in PvPR-1 gene expression were observed between the common bean contrasting varieties following C. lindemuthianum inoculation. Africano-4, the resistant one, showed a higher abundance of up-regulated and constitutively expressed PvPR-1 transcripts compared to its sensitive counterpart (Rosinha), indicating a more effective role of this gene family against the pathogen. Furthermore, based on PCA analyses and interaction networks of differentially expressed genes, three key targets within the PvPR-1 family (PvPR-1-4, PvPR-1-5, and PvPR-1-10) emerged as promising candidates for future functional characterization. These molecular actors displayed differential transcriptional patterns between the studied varieties without compromising the transcript abundance of PvPR-1 protein synthesis in the resistant one. Consequently, they may represent key components of resistance mechanisms that contribute to the differentiation between the two organisms. These findings deepen our understanding of PvPR-1 genes and their roles in common bean defense responses. They also emphasized the potential of PvPR-1 genes as candidates for breeding stress-resistant common bean varieties, which are crucial for bolstering crop resilience to environmental adversities.

The metabolic profiles of phenolic acids and aromatic amino acids in the Orobanche crenata parasite and its host faba bean at different infestation stages

Orobanche crenata is a root holoparasite that depends on its host for nutritional requirements. The shikimate pathway that metabolizes about 30% of the assimilated carbon in photosynthetic plants plays a role in host-parasite relationships. Aromatic amino acids (AAAs) and phenolic acids are derived from the shikimate pathway and serve as precursors of a wide number of primary and secondary metabolites. The effects of the O. crenata parasite on the phenolic acid and AAA profiles of two faba bean varieties and the differences of these compounds between the host organs and attached parasite at infestation stages were studied. Hosts and attached parasites were collected and divided, depending on the stage of parasite development, into four stages. The results revealed that phenolic acids and free AAAs tended to increase in parasitized roots compared to those of healthy roots, and the greatest increase in phenolic contents occurred at the first infestation stage. Syringic acid was observed to be unique to the parasite. Profiles of phenolic acids and AAAs were changed during the developmental stages of the parasite and differed from those of its host. Caffeic in the parasite reached more than 100 times that in Nubaria 4 host roots at the fourth infestation stage. Free phenylalanine in the parasite ranged between 2.2 and 5.5 times its level in host roots at all infestation stages. This study provides much evidence that indicates O. crenata is able to self-regulate its phenolic and AAA metabolites during its developmental stages, which differ from those of its host.

The influence of meteorological conditions of the Non-Chernozem zone on the phytosanitary condition of grain bean crops

There is a tendency to expand the area of bean cultivation in the northern direction, which is due to climatic changes and breeding achievements. There are 191 varieties of beans registered in the state register of breeding achievements approved for use, of which 31 varieties are recommended for cultivation in the belt between 50° and 60° latitude. The relevance of bean cultivation in the Non-Chernozem zone is increasing. This is due to the increased demand for beans, which are characterized by high nutritional value. Also, this crop is promising as an alternative in grain crop rotations. The study of the phytosanitary condition of bean plants in potentially new cultivation regions in order to develop a crop protection system is of interest. The promotion of crops to new territories is inevitably accompanied by phytosanitary risks, which have been little studied due to insufficient information about bean diseases. The literature provides an assessment of potential threats. However, their manifestation is closely related to the climatic and weather conditions of the growing season. The analysis of the interrelationships of the phytosanitary condition of crops with the temperature and humidity regimes of the bean growing period is relevant.

Migration of Plutonium, Micro- and Macroelements in the “Soil–Plant” System at Different Soil Moisture

In the vegetation experiment, the plutonium, micro- and macroelements migration in the “soil–agricultural plant” system depending on soil moisture in the range from 15 to 40٪ of absolute soil moisture were studied. The content of 239Pu was analyzed by α-spectrometry with preliminary radiochemical isolation. The elemental composition was analyzed by the ICP-MS and ICP-AES methods. Beans (Fabaceae) variety “Amber” were used as a test culture. The plutonium transfer factor obtained in the vegetation experiments are in the range of of 5.3×10–4–1.5×10–2, with an average value of 5.4×10–3 for the aboveground part of bean and range of 4.5×10–2–2.7×10–1, with an average of 1.6×10–1 for bean roots. It was determined that the distribution of plutonium, micro and macro elements in the vegetative organs of plants is not equally, the transfer factor of plutonium for the aboveground part of plants is lower than for the root part. It has been established that the accumulation of plutonium, micro- and macroelements, depending on soil moisture, is different for the organs of beans. The dependence of plutonium accumulation by plants on soil moisture is significantly higher than for other considered elements. A decrease in the coefficient of accumulation of plutonium in the aerial part of the beans is recorded with an increase in soil moisture up to two orders of magnitude. There is a trend towards a slight decrease in the accumulation coefficients of Fe, Mg, Mn, Cr, Mo, Ni, Co, Cu. For the root system of beans, a clear dependence of the accumulation of the considered elements on soil moisture is not observed.

Cooking Time, Seed Darkening, and Iron and Zinc Concentrations of Selected Andean Genotypes of Common Bean

ABSTRACTCooking time (CT), post‐harvest darkening (PHD), and micronutrient content influence consumer preference of common bean (Phaseolus vulgaris L.) varieties. The objective of this study was to evaluate Andean genotypes for CT, PHD, and seed iron and zinc concentration. A total of 52 genotypes belonging to three market classes, namely, purple‐speckled (Kabulangeti), yellows, and cranberry (sugar beans), were grown at the Golden Valley Agricultural Research Trust (GART) Farm and the Mpika Research Farm in Zambia and assessed for CT, PHD, and seed iron and zinc concentration. The fastest cooking genotype among all 52 genotypes was a yellow genotype, Y1612‐5. Yellow genotypes generally cooked faster than the Kabulangeti and sugar types. Among the elite lines with Kabulangeti seed types, which is a dominant market class in Zambia, the elite line K175 had faster CT and higher iron and zinc concentration than the Kabulangeti landrace race currently available on the Zambian market. Kabulangeti and sugar genotypes showed regular darkening, whereas yellows did not darken. The yellow genotype Y1612‐3 was particularly outstanding because of its unique combination of high concentrations of both iron and zinc. Therefore, it can be used to introgress these two essential minerals into variable genetic backgrounds. The significant variability and high to moderate heritability for CT (0.85) and iron (0.81) and zinc (0.58) concentration observed in the current study suggest that it is possible to make progress in breeding for faster cooking varieties that are rich in iron and zinc.

Chemical Profiling of Polar Lipids and the Polyphenolic Fraction of Commercial Italian Phaseolus Seeds by UHPLC-HRMS and Biological Evaluation

The common bean (Phaseolus vulgaris L.) is one of the oldest food crops in the world. In this study, the ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-MS/MS) technique was used to characterize the polar lipid composition and polyphenolic fraction of five bean varieties commonly consumed in Italy: Cannellino (PVCA), Controne (PVCO), Borlotti (PVBO), Stregoni (PVST), and Vellutina (PVVE). Lipid content represents a minor fraction of the whole metabolome in dry beans, and little is known about their polar lipids, which could be potentially bioactive components. Thirty-three compounds were detected through UHPLC-MS/MS, including oxylipins, phospholipids, N-acyl glycerolipids, and several fatty acids. The dichloromethane extracts were subjected to principal component analysis (PCA), with the results showing greater differentiation for the Borlotti variety. Moreover, 27 components belonging to different polyphenol classes, such as phenolic acids, flavonoids, catechins, anthocyanins and their glycosides, and some saponins, were identified in the hydroalcoholic seed extracts. In addition, the mineral content of the beans was determined. Considering the high number of compounds in the five apolar seed extracts, all samples were examined to determine their in vitro inhibitory activity against the enzyme cyclooxygenase-2 (COX-2), which is inducible in inflammatory cells and mediates inflammatory responses. Only PVCO showed the best inhibition of the COX-2 enzyme with an IC50 = 31.15 ± 2.16 µg/mL. In light of these results, the potential anti-inflammatory properties of PVCO were evaluated in the LPS-stimulated murine macrophage cell line J774A.1. Herein, we demonstrate, for the first time, that PVCO at 30 µg/mL can significantly reduce the release of TNF-α, with a less significant anti-inflammatory effect being observed in terms of IL-6 release.

Proteomic analysis of storage proteins in Phaseolus vulgaris associated with resistance to water stress

The common bean (Phaseolus vulgaris), a widely consumed legume in Ecuador, boasts low economic value, significant nutritional contributions, and a remarkable capacity to enhance soil fertility. Despite these attributes, its field productivity often needs to improve, with the water deficit emerging as a primary hindrance. Consequently, genetic enhancements have been incorporated into select varieties, conferring tolerance to specific levels of water scarcity stress. This study aimed to elucidate the distinctions in protein expression patterns responding to water deficiency stress across nine bean varieties. Protein patterns were scrutinized through two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and selected protein spots were subjected to mass spectrometry analysis (MALDI-TOF MS/RP-LC-MS/MS). A comprehensive identification of 111 proteins was achieved and categorized based on their respective functions. Noteworthy among these were the desiccation-protectant protein (LEA14) and Desiccation-related protein PCC13–62, identified as proteins associated with the response to abiotic stress, particularly prevalent in the INIAP_473 cultivar. These findings underscore the potential for targeted genetic improvements to mitigate the impact of water deficit stress on common bean cultivation, contributing to enhanced agricultural resilience and productivity.

Evaluation of Faba Bean (Vicia faba L.) Varieties for Yield and Chocolate Spot (Botrytis fabae) Disease in Western Tigray, Ethiopia

Faba bean (Vicia faba L.) has an important pulse crop in Ethiopian. It is consumed in various forms and contributes to soil fertility improvement. However, its production and productivity are challenged by biotic and abiotic constraints. Chocolate spot is known as Botrytis fabae, is a significant biotic constraint that negatively impacts faba bean crops in highland areas of western Tigray. The aim of the study mentioned was to assess faba bean varieties for their grain yield and resistance to chocolate spot disease under field conditions. Field experiment was conducted at Welkayt and Tsegedie district during 2017 cropping season using five released faba bean varieties and one local check. Treatments were evaluated with RCBD and replicated three times. There was significant difference (p<0.01) among tested faba bean varieties and lactation, while, interaction effect of varieties and lactation were non-significant variation (p<0.05). In the analysis conducted on grain yield from different varieties, it was observed that the variety Hachalu achieved the highest grain yield at 632kg/ha, followed closely by Tumsa with a yield of 611.8kg/ha. While, minimum yield was obtained from variety Obse (384.3kg/ha) at Welkayt. Similarly, in Tsegedie maximum yield was calculated from variety Tumsa (339.8kg/ha) and Hachalu (334.4kg/ha), respectively. Minimum yield was obtained from variety Obse (238.8kg/ha). There was significant variation among faba bean varieties in terms of PSI at a significance level of P<0.05, while disease incidence did not show significant differences among the varieties tested. Lowest mean disease severity was recorded from varieties Hachalu (15%) followed by Tumsa (17.7%). However, Highest mean value was recorded from Obse variety (30.02%) at Welkayt. Similarly, in Tsegedie lowest disease severity was recorded from varieties Hachalu (28.7%) and Tumsa (28.8%). Highest mean value recorded from varieties Obse (susceptible) (50.13). There was positively and significant correlation among yield and Agronomic traits. However, there was negatively significant yield with PSI at both locations. Varieties with low disease severity are useful in breeding program aimed at developing with higher resistance of chocolate spot disease.

Performance of dolichos bean (Lablab purpureus L.) varieties as influenced by growth stimulants on quality and economics under southern Telangana conditions

Performance of dolichos bean (Lablab purpureus L.) varieties as influenced by growth stimulants on quality and economics under southern Telangana conditions

Effect of Treatment with Humic Acid on Some Growth Characteristics and Protein Content in Different Varieties Vicia faba L. Plant

An experiment was carried out using plastic pots in the 2023-2024 season to evaluate the method of treatment with humic acid in the growth characteristics and protein percentage of different varieties of faba bean plants. The study included two factors, the method of adding humic acid (without acid, spraying acid on the plant, adding acid to the soil). The second factor included four varieties of beans (local Iraqi, Netherland, Spanish, and French). The results showed: superiority of the treatment of adding Humic acid to the soil in terms of plant height, total chlorophyll percentage, number of leaves, and protein percentage by 13.25% and 7.59 mg g-1 wet weight. 6.78% and 8.75%, respectively Compared to the control treatment, the local variety was superior in the characteristics of plant height and number of leaves by 31.72 and 7.26% compared to the French variety, which was superior in the characteristics of number of leaves and protein percentage by 12.27 and 24.0%, respectively. The interaction also outperformed adding acid to the local variety in plant height. By 53.13%, the French variety with added acid exceeded the characteristics of number of leaves and protein percentage by 26.40 and 30.50%, respectively.

Key Determinants of Local Bean Production and Marketing in Mbeya District, Tanzania

Common beans are a widely cultivated crop in East and Central Africa. This study aimed to assess the factors affecting local bean production and marketing at the household level. Using a non-experimental research design, the study surveyed 111 farmers from the Isangati division in Mbeya District. Cross-sectional data were collected through expert sampling methods, and regression analysis was employed to examine the influence of socio-economic factors on the production and marketing of local bean varieties. The results indicated that variables such as age, education, occupation, and farm size significantly influenced bean production (p<0.01), as did market accessibility and the acceptability of bean varieties (p<0.01). The study concluded that proximity to markets, socio-economic conditions, and consumer preferences play a crucial role in shaping bean production and marketing outcomes. As a result, interventions should be tailored to meet both user and consumer preferences.

Efficacy of Different Priming Methods on Germination and Growth of French Bean (Phaseolus vulgaris L.) Under Field Conditions in Mugu, Nepal

Low seed germination and poor growth have been major problems in French bean production in the hilly region of Nepal. An effective solution may lie in seed priming, which enhances rapid and uniform seed emergence, thereby improving yield. A field experiment was conducted to find out the effect of different seed priming methods on various varieties of French beans under field conditions in Chhaayanath Rara-4, Mugu, Nepal. The experiment was conducted in two factorial RCBD with three replications. Factor A was three French bean varieties; black, ghoratte, and red beans, and Factor B included two methods of priming; hydro and halo along with control. The results revealed that the germination parameter was significantly influenced by the method of priming and it was found non-significant in the case of varieties. Maximum germination percentage and higher speed of germination were found in seeds treated with hydro-priming. Similarly, the growth parameter was found significant for both varieties as well as priming methods. Vegetative growth parameters such as plant height, number of leaves, and number of primary and secondary branches were found significantly higher in hydro-primed seeds and ghoratte variety. Likewise, the yield parameters such as days to first flowering, days to 50% flowering, and yield per hectare were found significantly higher in hydro-primed ghoratte beans. Hence, hydro-priming can be used as a simple and cost-effective technique to improve germination and growth of French beans along with suitable variety under field conditions in Mugu district, Karnali province, Nepal. Int. J. Appl. Sci. Biotechnol. Vol 12(3): 137-144.

Improved Mung Bean (Vigna radiate L.) Technology Demonstration and Promotion Under North-West Ethiopia

North-West Ethiopia, particularly metekel area, was conducive for the production of lowland pulses including mung beans. However, because of some known and unknown factors the productivity of mung beans was not that much improved regardless of the study area’s potential. Due to this, a two season field experiment was conducted to demonstrate and promote improved mung bean varieties under pawe district for 2021 and 2022 consecutive cropping seasons. During 2021 and 2022 main seasons, demonstration and promotion of improved mung bean varieties with the direct involvement of stakeholders (farmers, agricultural experts, government officials, researchers) field day event was carried out. The improved mung bean varieties demonstrated and promoted were NVL-1, N-26 (Rasa), Shoarobit (as a check) and MH-97-6 (Borda). Small scale farmers and agricultural experts even district government officials were purposely selected from the nearby district (pawe) followed that theoretical trainings about the production of mung bean, were given during the event. Each event participants have set their own variety selection parameters; with this majority of the participants selected and promoted N-26 (Rasa), NVL-1 mung bean varieties based on high grain yield, more number of pods per plant, larger grain size, uniformity and earliness, the rest of the participants preferred Shoarobit (as a check) based on high biomass and grain yield whereas MH-97-6 (Borda) was selected and promoted based on medium duration and high yield. The author suggested that, those selected and promoted mung bean varieties (mainly N-26 (Rasa), NVL-1 and MH-97-6 (Borda) have been recommended for production under the study area and similar agro-ecologies to satisfy the seed demand raised by small scale farmers and other agents.

Effect of Different Levels of Uptake Stimulants on the Yield of Dolichos Bean (Lablab purpureus)

This case study investigates the impact of different levels of uptake stimulants on the growth and yield of a leguminous vegetable crop Dolichos bean (Lablab purpureus). The field experiment was conducted during the Rabi season of 2024 at agricultural land of ASPEE Foundation based in Palghar, Maharashtra. The study utilized a Randomized Block Design (RBD) with three replications and seven treatments. Each treatment received drenched well-formulated nutrients in different concentration. The highest pod yield was recorded 2.187 ton/ha with the application of 2 ml P star used per liter of water(T2). For the considered Bauni variety of Dolichos bean, the soil parameters of experimental field are as follows: pH 7.28, Organic Carbon (OC) 0.17%, Electrical Conductivity (EC) 0.84 dS/m, Nitrogen (N) 85.29 kg/ha, Phosphorus (P) 92.19 kg/ha, and Potassium (K) 394.17 kg/ha. This indicates that the application of appropriate treatments using appropriate crop protection equipment with integrated nutrient management (INM) significantly enhanced both growth and yield parameters of Dolichos bean such as number of pods per cluster, pod weight, number of seeds per pod, pod yield per plant and pod Yield (kg/ha).

Research on variety identification of common bean seeds based on hyperspectral and deep learning

Accurate, fast and non-destructive identification of varieties of common bean seeds is important for the cultivation and efficient utilization of common beans. This study is based on hyperspectral and deep learning to identify the varieties of common bean seeds non-destructively. In this study, the average spectrum of 3078 hyperspectral images from 500 varieties was obtained after image segmentation and sensitive region extraction, and the Synthetic Minority Over-sampling Technique (SMOTE) was used to achieve the equilibrium of the samples of various varieties. A one-dimensional convolutional neural network model (IResCNN) incorporating Inception module and residual structure was proposed to identify seed varieties, and Support Vector Machine (SVM), K-Nearest Neighbor (KNN), VGG19, AlexNet, ResNet50 were established to compare the identification effect. After analyzing the effects of multiple spectral preprocessing methods on the model, the study selected Savitzky-Golay smoothing correction (SG) for spectral preprocessing and extracted 66 characteristic wavelengths using Successive Projections Algorithm (SPA) as inputs to the discriminative model. Ultimately, the IResCNN model achieved the highest accuracy of 93.06 % on the test set, indicating that hyperspectral technology can accurately identify bean varieties, and the study provides a correct method of thinking for the non-destructive classification of multi-species small-sample bean varieties.

Biochemical Defense Arsenal, Genes/QTLs and Transcripts for Imparting Anthracnose Resistance in Common bean (Phaseolus vulgaris L.)

Anthracnose (ANT), caused by Colletotrichum lindemuthianum, is the most devastating disease affecting common bean (Phaseolus vulgaris L.), leading to significant yield losses in the Western Himalayas. The study provides a comprehensive understanding of ANT resistance via trait phenotyping, biochemical profiling, genome-wide association studies (GWASs), and RNA sequencing. The assessment of bean association mapping panel in different environments revealed a diverse spectrum of resistance levels. Biochemical analysis revealed distinctive defense responses against ANT infection among different genotypes. GWAS approach identified 24 significant marker‒trait associations (MTAs) distributed across all 11 bean chromosomes. Notably, 03 MTAs (BMr205, BMr269 and BMr244) present on chromosome Pv07 were validated for ANT, and the remaining MTAs were novel MTAs for ANT. Transcriptome sequencing of resistant (PBG-3) and susceptible (PBG-26) genotypes under mock and 120-hour post inoculation conditions revealed key differentially expressed genes, such as leucine-rich repeat domain-containing protein (PHAVU_007G087700g), NB-ARC domain-containing protein (PHAVU_003G002500g) and transcription factors pivotal for disease resistance. The expression patterns of four genes (PHAVU_007G087700g, PHAVU_003G002500g, PHAVU_007G056100g and PHAVU_003G003000g) were validated through quantitative reverse transcription polymerase chain reaction (qRT‒PCR). Furthermore, the integration of GWAS-identified candidate genes with transcriptomics and cross-referencing with previous studies validated overlapping regions and common candidate genes, enriching our understanding of the genetic basis of ANT resistance. Therefore, the results offer a holistic perspective on ANT resistance in common bean, providing a foundation for targeted breeding efforts. The identified potential candidate genes and associated pathways will contribute valuable insights into the development of ANT resistant common bean varieties.

Adaptation, Path Coefficient and Correlation Study of Yield and Associated Traits in Common Bean (Phaseolus vulgaris L.) Genotypes

The study evaluated new biofortified, high-yielding and disease-resistant bean varieties for their adaptability in Zimbabwe. The secondary objective was to assess the association between yield and other traits that contribute to grain yield and identify traits that have direct and indirect effects on grain yield. The study was conducted between December 2016 and June 2017 using an augmented design with 24 varieties replicated three times at Chinhoyi University of Technology Research Farm. Data was analysed using analysis of variance in the statistical analysis system (SAS) software. DAB482, DAB494 and DAB539 were identified as high-yielding varieties with desired disease tolerance to common bean blight (CBB), common leaf rust (CLR) and angular leaf spot). Therefore, these improved common bean varieties recommended for further multilocation evaluation with the potential to be released as commercial varieties. Correlation coefficient values and path analysis values (direct and indirect) showed that the weight of 100 randomly selected seeds per variety and the percentage number of plants that germinated per plot could be used for indirect selection for grain yield (kg/ha). A negative correlation indicates an inverse relationship between traits. This study observed significant negative correlations for CBB and CLR relative to grain yield (kg/ha). This suggests that the grain yield (kg/ha) decreases with increased disease incidence and severity, highlighting the importance of disease tolerance in achieving high grain yield. Hence, there is a need for the breeding program to select and advance varieties that are tolerant to diseases to achieve the desired improvement in grain yield performances.