Variation In Patterns Research Articles

Abstract A027: Ethnic variations in breast cancer risk factors distribution in Guam, HIPIMR study

Abstract Background: Guam has significant ethnic disparities in breast cancer incidence, with rates varying from 46.7 per 100,000 among Asian women to 137.5 per 100,000 among Micronesian women. Incidence rates for Filipino, CHamoru (indigenous people), and White women are 59.8, 94.0, and 121.9 per 100,000, respectively (2013-2017). The Hawai‘i and Pacific Island Mammography Registry (HIPIMR), a collaborative effort between the University of Hawai‘i Cancer Center and the University of Guam Cancer Research Center, collects health and breast imaging data to enhance early cancer detection tailored to the unique ethnic profiles of women in Hawai‘i and Guam. This study aims to identify the distribution of breast cancer risk factors among different ethnic groups in women undergoing screening in Guam. Differences in the prevalence of risk factors may explain variations in incidence patterns. Methods: This study included 1,413 women undergoing mammography screening at FHP Health Center in Guam, from November 2023 to May 2024. The risk factors were classified as follows: BMI (≥25), age of menarche (≤13), age of first pregnancy (≥30), number of live births (≤1), age of menopause (≥55), and family history of first-degree relatives. The robust Poisson method was used to estimate prevalence ratios (PR) of risk factors among ethnic groups, adjusted for age and education. Asians were used as the reference group due to their lowest incidence rates. Furthermore, the total risk score was calculated by summing all the risk factors. ANOVA was used to test the differences in average risk scores among different ethnic groups. Results: All ethnic groups had significantly higher prevalence of being overweight or obese compared to Asian women. The prevalence ratios were highest for Micronesians (PR=1.73, 95% CI: 1.40, 2.13) and CHamoru (PR=1.55, 95% CI: 1.27, 1.90). CHamoru women had the highest rates of breast cancer family history (PR=1.74, 95% CI: 1.19, 2.54). However, CHamoru women had a lower prevalence of having a small number of live births (PR=0.39, 95% CI: 0.24, 0.65) and an older age at first birth (PR=0.83, 95% CI: 0.71, 0.96) when compared to Asian women. There was a significant difference in average score among ethnic groups (P<.001). CHamoru women had significantly higher score (2.35) than Filipino (2.08) and Asian (2.00) women. Conclusion: Our study reveals disparities in risk factors across ethnic groups, consistent with the Guam Cancer Registry's reports of high breast cancer rates among Micronesian and CHamoru women. This project is ongoing, with the aim of linking our data with the Guam Cancer Registry. This linkage will enable us to identify breast cancer cases among participants and compare risk factors across different ethnic groups for women with and without breast cancer. Acknowledgement: This research was funded by the National Cancer Institute: The University of Guam/University of Hawaii Cancer Center Partnership to Advance Cancer Health Equity, Grant U54CA143728 (University of Guam, YCP)/Grant U54CA143727 (University of Hawaii Cancer Center). Citation Format: Gian Paras, Su Bin Jin, Rose Grino, Cabrini Aguon, John Shepherd, Grazyna Badowski. Ethnic variations in breast cancer risk factors distribution in Guam, HIPIMR study [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr A027.

Spatial and Temporal Variations of Total Suspended Matter Concentration during the Dry Season in Dongting Lake in the Past 35 Years

Dongting Lake is the second largest freshwater lake in China, located in the middle reaches of the Yangtze River. Since the 21st century, it has faced intensified human activities, particularly the Three Gorges Dam impoundment and sand mining. The water quality of Dongting Lake has significantly changed due to human activities and climate change. Currently, quantitative studies on the spatial–temporal variations of total suspended matter (TSM) during Dongting Lake’s dry season and the human impacts on its concentration are lacking. This study utilizes Landsat-5 TM and Landsat-8 OLI data to estimate the changes in TSM concentration during the dry season from 1986 to 2021, analyzing their spatial–temporal variations and driving mechanisms. By evaluating the atmospheric calibration accuracy and model precision metrics, we select a model based on the ratio of red to green band, achieving an R2 of 0.84, RMSE of 18.94 mg/L, and MRE of 27.32%. Applying this model to the images, we map the distribution of the TSM concentration during the dry season from 1986 to 2021, analyzing its spatial pattern and inter-annual variation, and further investigate the impacts of natural factors and human activities on the TSM concentration. Our results show the following: (1) From 1986 to 2021, the TSM concentration during the dry season ranges from 0 to 200 mg/L of Dongting Lake, with an area-wide average value between 41.61 and 75.44 mg/L. (2) The TSM concentration from 1986 to 2021 is significantly correlated with the water level. Before 2006, it correlates positively, but no significant correlation exists from 2006 onward. (3) From 2006 onward, the mean TSM concentration is notably decreased compared to that before 2006, likely due to the Three Gorges Dam, while our analysis indicates a significant positive correlation between the TSM concentration and sand mining intensity during this period. This study highlights the influence of the Three Gorges Dam and sand mining on the TSM concentration in Dongting Lake during the dry season, providing valuable insights for related research on similar lakes.

Pongo's ecological diversity from dental macrowear analysis

AbstractObjectivesOrangutans are found in tropical rainforests of Borneo (Pongo pygmaeus) and Sumatra (Pongo abelii and Pongo tapanuliensis), and they are primarily considered frugivorous species. However, ecological studies reported differences in feeding behavior between these species. P. pygmaeus spend more time feeding on low‐quality foods, such as bark and tough vegetation than do P. abelii. The aim of this study is to investigate if there is any geographic variation in molar macrowear pattern between the two species.MethodsWe analyzed the macrowear pattern of second mandibular molars of 58 orangutan specimens combining dental topographic measurements (occlusal relief index, percentage of dentine exposure, and percentage of enamel wear) with the occlusal fingerprint analysis method.ResultsThe molar macrowear patterns of P. abelii and P. pygmaeus show no significant differences in most of the variables examined in this study. While P. pygmaeus molars are generally characterized by flatter occlusal morphology with slightly larger crushing and grinding areas than those of P. abelii, these differences are not statistically significant. However, we do observe a more variable wear in P. pygmaeus.ConclusionsSimilarities in molar macrowear patterns between the two species could be linked to overlapping long‐term diets, primarily composed of ripe and pulpy fruits. Dental macrowear is a cumulative process that occurs during the individual's lifetime, and it may be difficult to detect seasonal changes through its examination. Future studies could integrate tooth micro‐ and macrowear data for a better understanding of primate dental ecology.

Seasonal variation of serum potassium and related prescription pattern: an ecological time series

AimsTo assess if ambient temperature-related effects on serum potassium levels impact clinical decision-making.MethodsThis study is an ecological time series consisiting of 1 218 453 adult patients with at least one...

Mortality Risk and Antibiotic Therapy for Patients with Infections Caused by Elizabethkingia Species-A Meta-Analysis.

Background and Objectives: Patients with infections caused by Elizabethkingia species require prompt identification and effective antibiotic treatment since these spp. are typically resistant to multiple antibiotics and variable susceptibility patterns. Understanding the mortality risk of this disease is difficult because of the relatively low incidence of infections caused by Elizabethkingia spp. and the lack of published systematic evaluations of the risk factors for mortality. The aim of the present study was to investigate risk factors for mortality in patients with infections caused by Elizabethkingia spp. by conducting a meta-analysis of existing studies on these infections. Materials and Methods: Studies comparing patients who died from infections caused by Elizabethkingia spp. with patients who survived were considered for inclusion. Studies that reported one or more risk factors for mortality were considered. Clinical predisposing variables, predisposing comorbidities, and clinical outcomes of antibiotic treatment were among the risk factors for mortality. Results: The meta-analysis included twenty studies with 990 patients, and 298 patients (30.1%) died. The following risk factors for mortality were identified: intensive care unit admission, the need for mechanical ventilation, immunosuppressive or steroid therapy use, pneumonia, comorbid liver disease, and the use of inappropriate antimicrobial therapy. Conclusions: The use of appropriate antimicrobial therapy is critical for the effective management of infections caused by Elizabethkingia spp. Antimicrobial susceptibility testing would be a more reliable means of guiding treatment. The identification of the best antimicrobial drugs is needed to ensure optimal treatment recommendations for treating Elizabethkingia-related infections.

Korean vowel harmony has weak phonotactic support and has limited productivity

Abstract Chong (2017) claims that a derived environment process is productive to the extent that it is supported by phonotactics. The present study tests this claim by comparing variable patterns observed in Korean vowel harmony of suffix alternation with vowel co-occurrence restrictions in the lexicon. A corpus study replicated findings of previous studies that the harmony in suffix alternation is losing productivity, conditioned by the quality of the stem vowel, the number of intervening consonants between the vowels and the stem class. Phonotactic generalisations in vowel sequences matched such tendencies in the alternation in that harmony was feeble in phonotactics and that some of the factors that modulate the harmony in alternation were found to affect the harmony in phonotactics as well. The findings generally support Chong’s claim that lack of phonotactic support for an alternation makes it harder to learn.

Quantitative analysis of the morphological variation within the tiger beetle Calomera littoralis (Fabricius, 1787) (Coleoptera: Cicindelidae) in Mongolia

For the past couple of centuries, much of tiger beetle taxonomic work has been focused on explaining intraspecific variation. In the Northern Hemisphere, over a thousand subspecies have been described and many have since been relegated to synonymy. Generally, the phenotypic-based subspecies circumscription has been purely descriptive. More quantitative and integrative analyses of the color and pattern variation in tiger beetles would be valuable. The geographically wide-ranging species, Calomera littoralis (Fabricius, 1987) contains 11 currently recognized subspecies, including two described from Mongolia that have been of questionable status due to the paucity of material available when they were described. Here, we assess the distribution of phenotypic variation within C. l. peipingensis (Mandl, 1934) and C. l. mongolensis (Mandl, 1981) to determine whether it best fits a pattern of two subspecies with a contact zone or a gradual cline, based on 494 specimens from 34 populations. Non-metric multidimensional scaling analyses and modeling of the fit between longitude and black dorsal phenotypes both indicate that the variation is best explained by a gradual cline of phenotype along an east-west gradient, not the presence of separate subspecies. As a result, we synonymize C. littoralis mongolensis, syn, nov. with C. littoralis peipingensis.

Evolutionary Systems Biology Identifies Genetic Trade-offs In Rice Defense Against Above- and Belowground Attackers.

Like other plants, wild and domesticated rice species (Oryza nivara, O. rufipogon, and O. sativa) evolve in environments with various biotic and abiotic stresses that fluctuate in intensity through space and time. Microbial pathogens and invertebrate herbivores such as plant-parasitic nematodes and caterpillars show geographical and temporal variation in activity patterns and may respond differently to certain plant defensive mechanisms. As such, plant interactions with multiple community members may result in conflicting selection pressures on genetic polymorphisms. Here, through assays with different above- and belowground herbivores, the fall armyworm (Spodoptera frugiperda) and the southern root-knot nematode (Meloidogyne incognita), respectively, and comparison with rice responses to microbial pathogens, we identify potential genetic trade-offs at the KSL8 and MG1 loci on chromosome 11. KSL8 encodes the first committed step towards biosynthesis of either stemarane- or stemodane-type diterpenoids through the japonica (KSL8-jap) or indica (KSL8-ind) allele. Knocking out KSL8-jap and CPS4, encoding an enzyme that acts upstream in diterpenoid synthesis, in japonica rice cultivars increased resistance to S. frugiperda and decreased resistance to M. incognita. Furthermore, MG1 resides in a haplotype that provided resistance to M. incognita, while alternative haplotypes are involved in mediating resistance to the rice blast fungus Magnaporthe oryzae and other pests and pathogens. Finally, KSL8 and MG1 alleles are located within trans-species haplotypes and may be evolving under long-term balancing selection. Our data are consistent with a hypothesis that polymorphisms at KSL8 and MG1 may be maintained through complex and diffuse community interactions.

Understanding disorder in monolayer graphene devices with gate-defined superlattices

Engineering superlattices (SLs)-which are spatially periodic potential landscapes for electrons-is an emerging approach for the realization of exotic properties, including superconductivity and correlated insulators, in two-dimensional materials. While moiré SL engineering has been a popular approach, nanopatterning is an attractive alternative offering control over the pattern and wavelength of the SL. However, the disorder arising in the system due to imperfect nanopatterning is seldom studied. Here, by creating a square lattice of nanoholes in the SiO2dielectric layer using nanolithography, we study the SL potential and the disorder formed in hBN-graphene-hBN heterostructures. Specifically, we observe that while electrical transport shows distinct SL satellite peaks, the disorder of the device is significantly higher than graphene devices without any SL. We use finite-element simulations combined with a resistor network model to calculate the effects of this disorder on the transport properties of graphene. We consider three types of disorder: nanohole size variations, adjacent nanohole mergers, and nanohole vacancies. Comparing our experimental results with the model, we find that the disorder primarily originates from nanohole size variations rather than nanohole mergers in square SLs. We further confirm the validity of our model by comparing the results with quantum transport simulations. Our findings highlight the applicability of our simple framework to predict and engineer disorder in patterned SLs, specifically correlating variations in the resultant SL patterns to the observed disorder. Our combined experimental and theoretical results could serve as a valuable guide for optimizing nanofabrication processes to engineer disorder in nanopatterned SLs.

The evolution of public attention in acute kidney injury and continuous renal replacement therapy: trends analysis from 2004 to 2024.

Acute kidney injury (AKI) and the need for Continuous Renal Replacement Therapy (CRRT) are critically important health concerns. This study analyzes global and regional Internet search queries to understand public attention in AKI and CRRT over time. We used Google Trends™ to analyze search queries for AKI and CRRT from January 2004 to March 2024. The study examined global trends and detailed insights from the United States, including state-by-state breakdowns. We identified patterns, peaks of attention, and temporal trends in public attention, comparing regional variations across the US and top-ranking countries worldwide. Global attention in AKI peaked in October 2022, with Portugal, Zambia, and Spain showing the highest regional attention. Within the United States, peak attention was in February 2008. Tennessee, Pennsylvania, and West Virginia were the top states that paid attention to AKI. Attention in CRRT peaked globally in March 2024. South Korea, Saudi Arabia, and Bahrain have led the global attention to CRRT. In the United States, peak attention was in April 2020. West Virginia, Tennessee, and Kentucky showed the highest state-specific attention in CRRT. This study reveals significant temporal and geographical variations in online search patterns for AKI and CRRT, suggesting evolving public attention to these critical health issues. This knowledge can guide the development of targeted public health initiatives, enhance medical education efforts, and help healthcare systems tailor their approach to improving awareness and outcomes in kidney health across diverse populations.

Notes on the natural history and identity of the pelagic sea snake Hydrophis platurus (Serpentes: Elapidae) from Golfo Dulce, Puntarenas, Costa Rica

Introduction: The pelagic sea snake Hydrophis platurus has the broadest distributional range of all the snakes and is the only sea snake of tropical waters off the west coast of the Americas. Within the variation in the color pattern of this species, completely yellow specimens were considered very rare or occasional. However, a yellow pattern dominates a population established in the interior of the Golfo Dulce in the Southern Pacific of Costa Rica. Objective: We studied the abundance, activity patterns, morphometry, and feeding behavior of the yellow H. platurus within the Golfo Dulce. Methods: Between February 2009 and July 2018, we conducted surveys for sea snakes from a boat within the perimeter of the Golfo Dulce. Results: Adults from the gulf population are smaller than the bicolor oceanic population, with females having larger bodies. Sea snakes within the gulf float and move with surface currents without being linked to drift lines and debris. These snakes are mainly found in clean, calm waters with little turbulence. They feed on a wide diversity of small fish, which they capture using passive stalking predatory behaviors floating on the surface. Prey are quickly captured and swallowed alive. This species has an almost total absence of predation, a pattern that extends to this yellow population. Conclusions: The characteristics of the gulf and the direction and strength of the surface currents at its entrance might restrict the transit of this yellow population to the outside and the entry of the bicolored snakes of the oceanic population. We do not consider making taxonomic changes in this Golfo Dulce population prudent. This yellow population is relatively common within the basin. However, the high tourist traffic and other activities are risk factors for its conservation. Protection policies must be created to preserve and protect this small population of yellow sea snakes unique within this species' distribution range.

Phase fraction measurement of gas-liquid flow based on cyclonic capacitive sensor (CCS) and inverse drift flux method

Abstract As the demand for clean energy sources like natural gas increases, the need for methods to measure the phase fraction of gas-liquid two-phase flows, which have complex and variable flow patterns, becomes more pronounced. This study introduces a phase fraction measurement method based on a cyclonic capacitive sensor. An effective phase fraction prediction model for the gas-liquid flow measurement section is established based on the capacitive signals. Using the drift flux principle, the dimensionless drift velocity of the spiral annular flow is analyzed, and a volume gas fraction (GVF) prediction model based on the ‘inverse drift flux method’ is established. Experimental results show that this method is suitable for a wide range of phase fraction measurements and is highly accurate. When the liquid volume fraction (LVF) is less than 10%, the average relative error of the predicted GVF is 0.3%; for the LVF from 10% to 30%, the average relative error is 1.4%.

Genetic Diversity, Kinship, and Polychromatism in the Spotted Eagle Ray Aetobatus ocellatus of Fiji

The spotted eagle ray Aetobatus ocellatus (Kuhl, 1923) has a widespread Indo-West Pacific distribution and displays substantial population genetic structuring. Genetic data are crucial for understanding the species’ diversity, connectivity, and adaptation. However, molecular genetic information on A. ocellatus from Melanesia is lacking, which impedes our understanding of gene flow among geographic regions. In this study, we sampled 45 A. ocellatus, primarily from Fiji’s largest fish market in the capital, Suva. Mitochondrial DNA Cytochrome C Oxidase subunit I (COI) barcoding was used for species identification, and DArT-seqTM technology was applied to assess the nuclear genetic diversity. Barcoding of the COI gene showed a 98.6% to 99.8% similarity to A. ocellatus reference sequences in the Barcode of Life Data System, and the 45 individuals were represented by three major evolutionary haplotype clusters. Genotyping resulted in 24,313 Single-Nucleotide Polymorphisms (SNPs) which were quality-filtered to 7094 SNPs per individual. The observed heterozygosity level was 0.310. The inbreeding coefficient was positive, and genotyping identified one full-sibling pair and one half-sibling pair from the 45 individuals. Additionally, eagle rays exhibit polychromatic patterns, and at least three ventral pattern variations were recorded in specimens from the market. Collectively, our main findings characterize the genetic profile of A. ocellatus in Fiji and can help to understand the diversification of this species within the region.

County-Level Enrollment in Medicare Advantage Plans Offering Expanded Supplemental Benefits

Since 2019 and 2020, Medicare Advantage (MA) plans have been able to offer supplemental benefits that address long-term services and supports (LTSS) and social determinants of health (SDOH). To examine the temporal trends and geographic variation in enrollment in MA plans offering LTSS and SDOH benefits. This cross-sectional study used publicly available data to examine changes in beneficiary enrollment and plan offerings of LTSS and SDOH benefits from the benefits data from the second quarter of each year and other data from April of each year except 2024, for which the first quarter was the latest for benefits data and January the latest for other data at the time of analysis. Multivariable linear regression models for each type of benefit were used to investigate associations between county characteristics and enrollment in 2024. Analyses were stratified for (1) Dual Eligible Special Needs Plans (D-SNPs) that exclusively enroll dual-eligible beneficiaries and (2) non-D-SNPs. The percentage of MA enrollees in plans offering LTSS or SDOH benefits at the county level. This study included 2 631 697 D-SNP and 20 114 506 non-D-SNP enrollees in 2020, which increased to 5 494 426 and 25 561 455, respectively, in 2024. From 2020 to 2024, the percentage of D-SNP enrollees in plans offering SDOH benefits increased from 9% to 46%, whereas the percentage fluctuated between 23% and 39% for LTSS benefits. There was an increase in non-D-SNP enrollees with LTSS (from 9% to 22%) and SDOH (from 4% to 20%) benefits from 2020 to 2023, which decreased in 2024. In 2024, the most offered LTSS benefit was in-home support services, and the most offered SDOH benefit was food and produce. The percentage of enrollees with these benefits varied across counties in 2024. In multivariable linear regression models, among D-SNPs, enrollment in plans offering any SDOH benefits was higher in counties with greater MA penetration (coefficient, 5.0 percentage points [pp] per 10-pp change; 95% CI, 2.1-7.9 pp), in urban counties (coefficient, 7.2 pp vs rural counties; 95% CI, 3.8-10.6 pp), in counties with greater enrollment in fully integrated D-SNPs (coefficient, 3.0 pp per 10-pp change; 95% CI, 2.2-3.9 pp), and in counties in states with approved Medicaid home- and community-based services waivers for individuals 65 years or older or those with disabilities (coefficient, 10.8 pp; 95% CI, 4.0-17.6 pp). Enrollment in D-SNPs offering LTSS benefits was also higher in counties with greater MA penetration (coefficient, 5.9 pp per 10-pp change; 95% CI, 2.4-9.5 pp), urban vs rural counties (coefficient, 4.6 pp; 95% CI, 1.1-8.1 pp), and counties with greater enrollment in fully integrated D-SNPs (coefficient, 3.0 pp per 10-pp change; 95% CI, 2.1-3.9 pp) in addition to counties with greater social vulnerability scores (coefficient, 1.4 pp per 10-pp change; 95% CI, 0.3-2.5 pp). In this cross-sectional study of MA plans and enrollees, an increase in enrollment was most consistent in D-SNPs offering SDOH benefits compared with LTSS benefits and in D-SNPs compared with non-D-SNPs. Geographic variation in enrollment patterns highlights potential gaps in access to LTSS and SDOH benefits for rural MA beneficiaries and dual-eligible enrollees living in counties with lower enrollment in fully integrated D-SNPs and states with more limited Medicaid home- and community-based services coverage.

Seasonal variation and group size effects in putty-nosed monkeys' (Cercopithecus nictitans) heterospecific associations in the Nouabalé-Ndoki National Park.

Many non-human primates form heterospecific associations to increase benefits resulting from group living like antipredation defence and increased foraging efficiency while avoiding costly resource competition that usually arises from large conspecific groups. Previous studies provided profound insight into how these benefits are obtained and what behavioural changes might be elicited through association formation. What remains widely unknown are factors that could account for intra-specific variation in association patterns. For instance, we are still widely lacking a comprehensive assessment of how group size and seasonality affect heterospecific associations across larger number of groups within a species. The current study monitored more than 20 groups of putty-nosed monkeys (Cercopithecus nictitans), a forest guenon known to be frequently in association with other monkey species, for 37months in the Nouabalé-Ndoki National Park, Republic of Congo. Amongst the five primate species observed in association with C. nictitans, grey-cheeked mangabeys (Lophocebus albigena) and crowned monkeys (C. pogonias) were the most frequently encountered association partners. We did not find any effect of seasonality on association rates. However, larger C. nictitans groups were substantially more in association with L. albigena and C. pogonias than smaller groups during the main dry season. We argue that our findings suggest a major impact of antipredation benefits of heterospecific troops including C. nictitans during periods of increased vulnerability. We discuss how knowledge about variations in association patterns may help to adjust conservation strategies.

Incorporation of pre- and intra-operative sentinel lymph node mapping techniques in dogs with apocrine gland anal sac adenocarcinoma

The high incidence of nodal metastasis, variable lymphatic drainage patterns, and prognostic significance of nodal metastasis in dogs with apocrine gland anal sac adenocarcinoma (AGASACA) highlight the need for development of standardized techniques for lymph node staging in canine AGASACA. The aim of this study was to develop and describe the utility of pre- and intra-operative sentinel lymph node (SLN) mapping techniques for subsequent nodal extirpation and histologic assessment in dogs with AGASACA. A prospective clinical trial was performed as a pilot study. Eight client-owned dogs with unilateral AGASACA were enrolled. Preoperative contrast-enhanced ultrasound (CEUS) via transrectal ultrasound (TRUS) and CT-lymphography (CTL) was followed by intraoperative SLN mapping within 7 days utilizing a visible dye (methylene blue [MB]) and a near-infrared (NIR) fluorescent dye (indocyanine green [ICG]) with subsequent nodal extirpation and routine anal sacculectomy. In all dogs, preoperative CTL and intraoperative SLN mapping identified at least one SLN. Pre- and intra-operative findings differed in 4/8 dogs. Preoperative CEUS identified a SLN in 7/8 dogs, but the exact location of the SLN was indeterminate. Extirpated lymph nodes were metastatic in 2/8 dogs. Preoperative CTL and intraoperative MB and ICG were an effective combination of SLN mapping techniques in dogs with AGASACA. These techniques each provided unique advantages. Combined, they identified and guided extirpation of early metastatic lymph nodes. Large-scale, prospective studies utilizing the techniques described are needed to accurately determine the incidence and significance of early/occult nodal metastasis in canine AGASACA.

Mystus vittatus (Hamilton, 1822)’un doğal ve kültür ortamındaki boy-ağırlık ilişkisi ve kondisyon faktörü

The Asian catfish Mystus vittatus is very popular food and ornamental fish in India. Length- weight relationships and condition factors of this fish are crucial component in fishery managements. The objective of this study is to investigate the relationships between the length, weight, condition factor (K), relative condition factor (Kn) and modified condition factor of Mystus vittatus (Hamilton, 1822). The study is particularly focus on analysing the sex wise variations in growth patterns, productivity, stocks, and conservation in two different habitats, natural habitat and biofloc system. The findings show that the species did not strictly follow the predicted cube law and showed allometric growth patterns in both habitats. The "b" value varies from 2.72 to 3.12, the condition factor (K) ranges from 0.587-1.50, modified condition factor ranges 0.54-2.26 and relative condition factor (Kn) ranges from 0.71-1.65. Natural habitats had the highest mean values for the regression parameter (b), condition factor (K), and relative condition factor (Kn). For male, female, and combined sexes in two different habitats, the R2 values show a statistically significant relationship between weight and length. The correlation analysis indicates some positive and negative significant relationships among sex, length, weight, condition factor, and relative condition factor. Therefore, there is significant relationship between condition factor, relative condition factor length, weight in two different habitats and it varies between male and female within same habitat. Thus, this finding will help to fishery managers for long term strategy development of Mystus vittatus in both natural as well as biofloc system.

Concordance of HER2 Expression in Paired Primary and Metastatic Sites of Endometrial Serous Carcinoma and the Effect of Intratumoral Heterogeneity

Primary endometrial serous carcinoma, known for its aggressive nature and poor prognosis, shares similarities with breast and gastric cancers in terms of potential HER2 overexpression as a therapeutic target. Assessing HER expression is complicated by tumor heterogeneity and discrepancies between primary and metastatic sites. In this study, we retrospectively analyzed HER amplification and expression in 16 pairs of primary endometrial serous carcinoma resections and corresponding metastases. HER2 status was determined using immunohistochemistry (IHC), with criteria based on the percentage and intensity of tumor cell staining. Confirmatory techniques, such as dual in situ hybridization (DISH) and fluorescence in situ hybridization (FISH), were also employed. This study reports on the concordance rates and the presence and pattern of HER2 heterogeneity. Our results showed an 87.5% concordance rate in HER2 amplification status between primary and metastatic sites, with 33% of cases scored as 2+ being amplified. Heterogeneity was observed in 100% of amplified cases and 95% of non-amplified cases on in situ testing, with variations in heterogeneity patterns between techniques. In conclusion, our findings emphasize the importance of testing both primary and metastatic sites or recurrences, with a concordance rate of 87.5%. In addition, a review of the literature and combining the results showed a concordance rate of up to 68%. The presence and pattern of heterogeneity, particularly in cases of mosaic or clustered heterogeneity in the primary tumor, may serve as reliable indicators of concordance, predicting a non-amplified HER2 status in corresponding metastases.

Patterns and Clinicopathological Features of Histologically Proven Metastases in Hepatocellular Carcinoma.

Hepatocellular carcinoma (HCC) is the most common primary liver malignant neoplasm. Multiple risk factors have been identified for several decades for this overly aggressive tumor. HCC is an overly aggressive malignancy with frequent intrahepatic and extrahepatic metastasis. In our practice, we have observed that HCC has the propensity to metastasize to very unusual sites and can sometimes show variable patterns leading to diagnostic difficulty. In this study of 257 patients, we aim to discuss the unusual sites of HCC metastasis, the various patterns of metastasis, clinicopathological features, and the most common cause of HCC in our population. In the course of our research study, we systematically extracted a comprehensive dataset comprising 257 instances of metastatic HCCs from the hospital database spanning the period from 2016 to February 2022. The assessment of metastatic sites uncovered a wide range of locations, reflecting significant diversity. The most common location was bone, with 135 cases (52.5%). The vertebral column was the most common location among bony metastasis, with 63 cases (24.7%). Morphologically, the most common histological pattern observed was pure trabecular in 192 patients (74.7%). All cases were diagnosed with the help of immunohistochemical stains. Out of 257 cases, 29.18% were diagnosed using glypican-3 and HepPar1, while 26.1% relied solely on HepPar1 positivity. HepPar1 was performed in a total of 240 cases, and positivity was seen in 205 cases (85.5%). In summary, our study represents the most comprehensive investigation of clinicopathological characteristics in metastatic HCC conducted within the past 20 years. It helps understand the histological and immunohistochemical features useful for diagnosis at metastatic sites for tumors with an unknown primary.

393 The role of on-animal sensors in understanding feed intake, behavior, and performance of grazing livestock

Abstract Intake by grazing animals is difficult to reliably measure in the field, particularly in more diverse grazing systems where pastures vary greatly across time and space in resource availability, which in turn leads to greater variation in animal time budgets and intake patterns. Advances in remote livestock monitoring (e.g., on-animal sensors, walk -over-weighing), and advanced analytics such as machine learning algorithms can contribute to the development of robust, adaptable tools for better understanding of animal performance and behavior in grazing systems. Many existing tools for predicting livestock production require estimates of feed intake, and while estimates based on forage sward characteristics have been developed and used for temperate, relatively homogenous pastures, they are less well defined for more diverse rangelands. These diverse pastures are more difficult to characterize due to their greater variation in quantity and quality; consequently, animal selectivity is greater in more variable pastures. However, such feedback loops between pasture diversity and animal behavior may not yet be adequately represented in biophysical models. On-animal sensors such as accelerometers and GPS devices can be used to develop new ways of assessing relative intake by providing accurate estimates of animal location and behavior profiles, such as the use of machine learning algorithms to detect how much time is spent grazing, walking, ruminating, or resting. Measurements of animal behavior could then be combined with other animal-based methods such as walk-over-weighing and fecal NIR to improve estimates of intake and production for a wide range of extensive grazing environments. Sensor-derived measurements of animal movement could also be used to improve estimates of the energetic costs of grazing, thereby improving estimates of energy available for growth. As an example, data on recent activity and paddock use from on-animal sensors could be entered into a model to estimate current feeding requirements, then combined with weather and pasture quality forecasts. The ensuing baseline could then be used to explore the potential variability of these projections and generate a range for possible expected performance over the next month or season. This approach could be repeated over time, and animal performance and behavior measures used to update model estimates and improve forward projections. These methods could also be used in conjunction with estimates of animal energy requirements to provide real-time prediction of relative feed intake, which would provide a step-change in modelling livestock production in extensive grazing systems. For these approaches to be successful, additional calibration data are needed across a wide variety of environments and species, and the tools must be cost-effective to implement. In summary, on-animal measurements provide an adaptable, data-driven approach that could be integrated with existing tools to improve our understanding of feed intake, behavior, and performance of grazing animals.