Variable Nutrient Research Articles

Soil macro‐ and mesofauna‐mediated litter decomposition in a subtropical karst forest

AbstractThis study aims to quantify the effect of different soil fauna on forest litter decomposition processes under natural conditions in the Maolan karst area of southwest China. Leaf litters of two typical evergreen species (CG, Cyclobalanopsis glauca and KS, Kmeria septentrionalis) were selected to conduct a field decomposition experiment using the mesh bags with three different apertures (5, 2, and 0.03 mm). Mass remaining and nutrient variation in the litterbags were determined for one year at 3‐month intervals. The results showed that litter decomposition rate in different mesh sizes litterbags were in the order of 5 mm >2 mm >0.03 mm. Soil macrofauna had a significant effect on litter decomposition in the two types of litter and the mixture of the two. The most considerable mesofauna effect was found in the KS litter, which is 36.5% ascribed by the lowest C/N ratio (high quality). Soil mesofauna had a more significant effect on N, which was time‐dependent. Moreover, the fauna richness and abundance were affected by the decomposition time but not limited by the litter quality. This study illustrated that soil macrofauna has a significant influence on litter decomposition. The effect of mesofauna on litter mass loss and nitrogen release are determined by litter quality in the subtropical karst forest ecosystems.Abstract in Chinese is available with online material.

Soil C, N and P contents and their stoichiometry as affected by typical plant communities on steep gully slopes of the Loess Plateau, China

The contents and stoichiometric characteristics of soil carbon, nitrogen and phosphorus greatly influence the structures and functions of the ecological system. The steep gully slope is the main source of erosion sediment in the small watershed on the Loess Plateau, China. It is vital to fully quantify the potential effects of vegetation restoration on soil nutrients and their stoichiometry under such harsh standing environments. To investigate the effects of plant communities on soil C, N, P contents and their stoichiometry on steep gully slopes. Two shrubs (Hippophae rhamnoides Linn., Caragana korshinskii Kom.), four typical grasses (Carex lanceolata Boott., Artemisia giraldii Pamp., Bothriochloa ischcemum (Linn.) Keng. and Artemisia sacrorum Ledeb.), and one slope farmland planted with Zea mays (as control) were chosen as the testing sites. Soil samples were collected from seven layers (0–10, 10–20, 20–30, 30–40, 40–60, 60–80, and 80–120 cm). The results showed that grassland had higher soil total carbon, soil total nitrogen, soil total phosphorus, and stoichiometries than shrubland on steep gully slopes. The C/N ratio had the minimum variation between different communities, while N/P and C/P ratios gradually decreased with soil depth. Both N and P were scarce on steep gully slopes. Soil C, N, P contents and their stoichiometry were closely related to bulk density, plant litter density and root mass density. The results are helpful to understand the spatial variation of soil nutrients and their stoichiometry and vegetation management on steep gully slopes in arid and semi-arid regions.

Temperatures Outside the Optimal Range for Helicobacter pylori Increase Its Harboring within Candida Yeast Cells.

Simple SummaryHelicobacter pylori is associated with the development of diverse gastric pathologies. This bacterium has been shown to invade yeast to protect itself from environmental factors such as changes in pH, the presence of antibiotics or variations in nutrients that affect their viability. However, intra-yeast H. pylori has been reported from other sources, including food, or when the storage temperature is outside the optimal growth range for H. pylori, which is 30–37 °C. It is necessary to continue investigating the environmental factors that participate in the entry of the bacteria into yeast. In this work, it was evaluated whether temperature changes promote the entry of H. pylori into Candida and whether this endosymbiosis favors bacterial viability. It was observed that H. pylori significantly increased its invasiveness to yeast when these two microorganisms were co-cultured under 40 °C. The results support that H. pylori invades yeasts to protect itself from stressful environments, favoring its viability in these environments. In addition, it can be suggested that this microorganism would use yeast as a transmission vehicle, thereby contributing to its dissemination in the population. However, the latter still needs to be confirmed.Helicobacter pylori is capable of entering into yeast, but the factors driving this endosymbiosis remain unknown. This work aimed to determine if temperatures outside the optimal range for H. pylori increase its harboring within Candida. H. pylori strains were co-cultured with Candida strains in Brucella broth supplemented with 5% fetal bovine serum and incubated at 4, 25, 37 or 40 °C. After co-culturing, yeasts containing bacteria-like bodies (Y-BLBs) were observed by optical microscopy, and the bacterium were identified as H. pylori by FISH. The H. pylori 16S rRNA gene was amplified from the total DNA of Y-BLBs. The viability of intra-yeast H. pylori cells was confirmed using a viability assay. All H. pylori strains were capable of entering into all Candida strains assayed. The higher percentages of Y-BLBs are obtained at 40 °C with any of the Candida strains. H pylori also increased its harboring within yeast in co-cultures incubated at 25 °C when compared to those incubated at 37 °C. In conclusion, although H. pylori grew significantly at 40 °C, this temperature increased its harboring within Candida. The endosymbiosis between both microorganisms is strain-dependent and permits bacterial cells to remain viable under the stressing environmental conditions assayed.

Coloration and phenology manifest nutrient variability in senesced leaves of 46 temperate deciduous woody species

Abstract Senesced-leaf nutrient concentrations vary significantly among coexisting plant species reflecting different leaf nutrient use strategies. However, interspecific variation in senesced-leaf nutrients and its driving factors are not well understood. Here, we aimed to determine interspecific variation and its driving factors in senesced-leaf nutrients. We explored interspecific variation in carbon (C), nitrogen (N) and phosphorus (P) concentrations in newly fallen leaves of 46 coexisting temperate deciduous woody species across the Maoershan Forest Ecosystem Research Station, Northeast China. The relative importance of 10 biotic factors (i.e. mycorrhiza type, N-fixing type, growth form, shade tolerance, laminar texture, coloring degree, coloring type, peak leaf-coloration date, peak leaf-fall date and end leaf-fall date) was quantified with the random forest model. N and P concentrations varied 4- and 9-fold among species, respectively. The high mean N (15.38 mg g−1) and P (1.24 mg g−1) concentrations suggested a weak N and P limitation in the studied forest. Functional groups had only significant effects on specific nutrients and their ratios. P concentration and N:P were negatively correlated with peak and end leaf-fall dates for the ectomycorrhiza species group. Brighter-colored leaves (red > brown > yellow > yellow-green > green) tended to have lower N and P concentrations and higher C:N and C:P than darker-colored leaves. The random forest model showed that autumn coloration and leaf-fall phenology contributed 80% to the total explanation of nutrient variability among species. The results increase our understanding of the variability in senesced-leaf nutrients as a strategy of woody plant nutrition in temperate forests.

Estimation of Populuseuphratica Forest Leaf Litterfall and Time Variation of Nutrient in Leaf Litter during Decomposition along the Main Channel of the Tarim River, China

Accurate determination of annual leaf litter amount constitutes the basis of scientific leaf litter nutrient release assessment. In this study, we tried to establish an equation between leaf litter amount and relevant tree characteristics of Populus euphratica (P. euphratica) tree on an individual scale, and to find the leaf litter nutrient content variation within 760 d incubation experiment in the main channel of the Tarim River, China. Results showed that there was no proper equation between leaf litter amount and tree height or diameter at breast height. There was great difference in leaf litter amount on an individual scale. The mean annual leaf litter amount per tree was 10.2, 14.83 kg/y obtained by field survey and the equation between annual leaf litter amount and canopy area on an individual scale, respectively. Leaf litter mass changed over incubation time and exhibited three main phases: an initial slow decomposition phase (0–173 d) with mass loss; a rapid mass loss phase (173–290 d); and a second rapid mass loss phase (470–560 d). Overall, carbon (C) and potassium (K) content decreased, and nitrogen (N) and phosphorus (P) content increased in a fluctuating manner over time in the P. euphratica leaf litter.

Identifying Nutrient Patterns in South African Foods to Support National Nutrition Guidelines and Policies.

Food composition databases (FCDBs) provide the nutritional content of foods and are essential for developing nutrition guidance and effective intervention programs to improve nutrition of a population. In public and nutritional health research studies, FCDBs are used in the estimation of nutrient intake profiles at the population levels. However, such studies investigating nutrient co-occurrence and profile patterns within the African context are very rare. This study aimed to identify nutrient co-occurrence patterns within the South African FCDB (SAFCDB). A principal component analysis (PCA) was applied to 28 nutrients and 971 foods in the South African FCDB to determine compositionally similar food items. A second principal component analysis was applied to the food items for validation. Eight nutrient patterns (NPs) explaining 73.4% of the nutrient variation among foods were identified: (1) high magnesium and manganese; (2) high copper and vitamin B12; (3) high animal protein, niacin, and vitamin B6; (4) high fatty acids and vitamin E; (5) high calcium, phosphorous and sodium; (6) low moisture and high available carbohydrate; (7) high cholesterol and vitamin D; and (8) low zinc and high vitamin C. Similar food patterns (FPs) were identified from a PCA on food items, yielding subgroups such as dark-green, leafy vegetables and, orange-coloured fruit and vegetables. One food pattern was associated with high sodium levels and contained bread, processed meat and seafood, canned vegetables, and sauces. The data-driven nutrient and food patterns found in this study were consistent with and support the South African food-based dietary guidelines and the national salt regulations.

Effects of environmental parameters on Lemna minor growth: An integrated experimental and modelling approach

Pollution of surface waters is a worldwide problem for people and wildlife. Remediation and phytoremediation approaches can offer a solution to deal with specific scenarios. Lemna minor, commonly known as duckweed, can absorb and accumulate pollutants in its biomass. To evaluate if L. minor could be applied for phytoremediation purposes, it is necessary to further investigate its remediation capability and to identify which parameters affect the remediation process. Such a model must include both plant growth and pollutant exchange. A remediation model based on a robust experimental study can help to evaluate L. minor as a proper remediation strategy and to predict the outcome of a L. minor based remediation system. To set up this model, this paper focusses on a detailed experimental study and a comprehensive mathematical modelling approach to represent L. minor growth as a function of biomass, temperature, light irradiation and variable nutrient concentrations. The influence of environmental conditions on L. minor growth was studied, by composing 7 days growth curves. Plants were grown under predefined environmental conditions (25°C, 14h photoperiod, 220 μmol m−2 s−1 light intensity and a modified Hoagland solution with 23.94 mg N L−1 and 3.10 mg P L−1 (N:P ratio of 7.73)) as standard for all experiments. The influence of different temperatures (6, 10, 15, 20, 25, 30 and 35°C), light intensities (63, 118, 170, 220 and 262 μmol m−2 s−1), photoperiods (12h and 14h) and N:P ratios (1.18, 3.36, 7.73 and 29.57) were tested in the model. As a result, a growth model was optimised using separate datasets for temperature, light intensity, photoperiod and nutrients and validated by further integrated testing. The growth model is a stable platform for application in phytoremediation of radionuclides in contaminated water, to be extended in future studies with information of pollutant uptake, pollutant-nutrient interactions and transfer to the biomass.

Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

AbstractHullessbarley (Hordeum vulgare L.) is a barley cultivar, serving as one of the major food crops worldwide. The main nutrients in hulless barley are starch, protein, and β‐glucan, the contents of which vary considerably among different cultivars. However, the potential molecular mechanisms that underlie these variations in nutrient are still insufficiently clear. In this study, the nutrient content of two hulless barley cultivars (‘Zangqing 2000’ and ‘C2’) was analyzed, and quantitative tandem mass tag‐LC‐MS/MS proteomics and bioinformatics analysis were used to verify the differentially expressed proteins (DEPs) between the two cultivars. Parallel reaction monitoring (PRM) analysis was used to verify the expression change of candidate DEPs. The content of starch and amylose in C2 was lower than that in Zangqing 2000, and β‐glucan, protein content, and amylopectin showed a higher concentration in C2 hulless barley cultivar. A total of 326 DEPs were identified, with 108 induced proteins and 218 reduced proteins in C2 compared with Zangqing 2000. Further functional and pathways analysis of DEPs revealed that C2 hulless barley cultivar has relatively more biological activities, and DEPs in C2 hulless barley cultivar were mainly associated with the biological processes of multiple important metabolic pathways. Moreover, PRM analysis showed the accuracy level of quantitative proteomic data. Overall, this was the first study that undertook the proteomic analysis in Zangqing 2000 and C2 and the results revealed the molecular mechanisms of seed nutrient biosynthesis and accumulation, which provide key insights about the development of hulless barley quality.

Compositional Shift of Bacterial, Archaeal, and Fungal Communities Is Dependent on Trophic Lifestyles in Rice Paddy Soil.

The soil environment determines plants’ health and performance during their life cycle. Therefore, ecological understanding on variations in soil environments, including physical, chemical, and biological properties, is crucial for managing agricultural fields. Here, we present a comprehensive and extensive blueprint of the bacterial, archaeal, and fungal communities in rice paddy soils with differing soil types and chemical properties. We discovered that natural variations of soil nutrients are important factors shaping microbial diversity. The responses of microbial diversity to soil nutrients were related to the distribution of microbial trophic lifestyles (oligotrophy and copiotrophy) in each community. The compositional changes of bacterial and archaeal communities in response to soil nutrients were mainly governed by oligotrophs, whereas copiotrophs were mainly involved in fungal compositional changes. Compositional shift of microbial communities by fertilization is linked to switching of microbial trophic lifestyles. Random forest models demonstrated that depletion of prokaryotic oligotrophs and enrichment of fungal copiotrophs are the dominant responses to fertilization in low-nutrient conditions, whereas enrichment of putative copiotrophs was important in high-nutrient conditions. Network inference also revealed that trophic lifestyle switching appertains to decreases in intra- and inter-kingdom microbial associations, diminished network connectivity, and switching of hub nodes from oligotrophs to copiotrophs. Our work provides ecological insight into how soil nutrient-driven variations in microbial communities affect soil health in modern agricultural systems.

Soil phosphorus accumulation in mountainous alpine grassland contributes to positive climate change feedback via nitrifier and denitrifier community

Mountainous alpine ecosystems are sensitive to global change, where soil nutrient content would potentially vary under current climate change background, and thus possibly influence the activity of nitrifiers and denitrifiers, as well as N2O emissions. However, within mountainous alpine ecosystems, the potential variation of soil nutrients under current global change and the consequence to N2O emission from nitrification and denitrification are still unclarified, hampering a comprehensive understanding of the feedback mechanisms between the nitrogen cycle and climate change. In order to fill this knowledge gap, we selected alpine grasslands at three different elevations and investigated the distribution and environmental drivers of nitrifiers and denitrifiers. The results showed that the lowest elevation site tended to have higher total phosphorus (TP) accumulation within the topsoil. The abundance of functional groups, emission of CO2 and N2O, and the N2O/CO2 ratio showed a decreasing trend along elevation. TP was the greatest influence on denitrifier composition (nosZ/narG and nirS/nirK ratios) and considerably influenced nitrifier composition (AOA/AOB ratio), and was significantly correlated to the N2O/CO2 ratio. In microcosms of soils from the highest elevation site, TP addition decreased the ratios of nosZ/narG, nirS/nirK, and AOA/AOB, and increased N2O/CO2 ratio and N2O emission, thus contributing to positive climate change feedback. This study indicates the potential for change within the nitrifier and denitrifier communities under current climate change, and highlights the role TP plays in governing nitrification and denitrification in mountainous alpine ecosystems.

Effects of Tree Species and Soil Enzyme Activities on Soil Nutrients in Dryland Plantations

Long-term afforestation strongly changes the soil’s physicochemical and biological properties. However, the underlying mechanism of different tree species driving change in soil nutrients is still unclear in the long-term dryland plantations of the Loess Plateau, China. In this study, samples of surface soil (0–20 cm) and woody litter were collected from five plantations (≥50 years) of Caragana korshinskii, Armeniaca sibirica, Populus hopeiensis, Platycladus orientalis, and Pinus tabulaeformis and a natural grassland, and tested for the carbon, nitrogen, phosphorus, and potassium contents, as well as the soil sucrase (SC), urease (UE), and alkaline phosphorus (ALP) activities. We found that soil nutrients, enzyme activities, and the litter’s chemical properties obviously varied among five tree species. C. korshinskii significantly increased the soil’s TC, organic carbon (OC), total nitrogen (TN), available nitrogen (AN), and available potassium (AK) by 28.42%, 56.08%, 57.41%, 107.25%, and 10.29%, respectively, and also increased the soil’s available phosphorus (AP) by 18.56%; while P. orientalis significantly decreased soil TN (38.89%), TP (30.58%), AP (76.39%), TK (8.25%), and AK (8.33%), and also decreased soil OC (18.01%) and AN (1.09%), compared with those in grassland. The C. korshinskii plantation had higher quality litter and soil enzyme activities than the P. orientalis plantation. Moreover, 62.2% of the total variation in soil nutrients was explained by the litter’s chemical properties and soil enzyme activities, and the litter phosphorus (LP) and soil ALP had a more significant and positive impact on soil nutrients. Therefore, tree species, LP, and soil ALP were key factors driving soil nutrient succession in dryland plantations. The significantly positive nitrogen–phosphorus coupling relationship in the “litter–enzyme–soil” system revealed that the improving nitrogen level promoted the phosphorus cycle of the plantation ecosystem. Our results suggest that leguminous tree species are more suitable for dryland afforestation through the regulation of litter quality and soil enzyme activities.

Positive effects of applying endophytic bacteria in eggplant-Sedum intercropping system on Cd phytoremediation and vegetable production in cadmium polluted greenhouse

The combination of intercropping and phytoremediation in the remediation of cadmium contaminated soil is an emerging model in recent years, but the results of previous studies are inconsistent. In the field experiment, eggplant was intercropped with hyperaccumulator Sedum alfredii Hance (inoculated or not inoculated with endophytic bacteria) to study the effects of intercropping on vegetable safety production, phytoremediation efficiency of hyperaccumulator and variation of soil available nutrients. The results showed that the intercropping treatment had a negative effect on the growth of eggplant and Sedum, but endophyte SaMR12 alleviated the inhibition of intercropping on plant growth. Intercropping treatment increases the Cd concentration in edible part of eggplant to 1.34 mg/kg compared with eggplant monoculture (1.19 mg/kg). While the application of SaMR12 reduces the Cd concentration of eggplant fruit to 0.95 mg/kg and significantly promotes the Cd uptake by Sedum. What's more surprising is that compared with eggplant monocropping, the content of soil available nitrogen, phosphorus and potassium in the treatment of intercropping with inoculated Sedum increased significantly. And according to the correlation analysis of various indexes of plants and soil, the Cd content of eggplant is negatively correlated with the available phosphorus and potassium in the soil, while the Cd content of Sedum is positively correlated with it, which suggested that the application of phosphorus and potassium fertilizers in this experimental site was beneficial to reduce Cd content in eggplant and improve Cd phytoextraction of Sedum. Therefore, in the daily production of moderately Cd-contaminated soil, intercropping eggplant with Sedum inoculated with endophytic bacteria is an excellent Phytoextraction Coupled with Agro-safe-production (PCA) pattern.

Altering Nitrogen Sources Affects Growth Carbon Costs in Vachellia nilotica Growing in Nutrient-Deficient Grassland Soils.

Vachellianilotica (L.) Willd. Ex Del. is a multipurpose leguminous tree that is common in grassland and savanna ecosystems in southern and eastern Africa. These ecosystem soils are reported to be acidic and nutrient-limited, specifically with regards to nitrogen (N) and phosphorus (P). The presence of this plant in these terrestrial ecosystems improves soil fertility benefiting the surrounding vegetation due to its ability to fix atmospheric N. This study seeks to understand the N-fixing bacteria symbiosis and physiological adaptations of V. nilotica in these acidic and nutrient-deficient KwaZulu-Natal soils. The soils used for this study were collected from the Ukulinga Grassland Nutrient Experiment located at the Ukulinga research farm of the University of KwaZulu-Natal, Pietermaritzburg, South Africa. Due to long-term soil nutrient addition treatments, these soils offered a diverse nutrient variation for better understanding the effects of acidity and nutrient variation on microbial symbiosis, plant nutrition, and biomass accumulation of V. nilotica. V. nilotica was able to maintain growth by relying on both atmospheric and soil-derived N across all treatments decreasing carbon (C) growth costs. There was an increased reliance on atmospheric-derived N of un-nodulated high N-treated plants. The plants grown in high N + P soils were able to nodulate with various species from the Mesorhizobium genus, which resulted in increased biomass compared to other plants. The results of this study show that V. nilotica can alter N sources to reduce C growth costs. In addition, both nodulating and free-living soil N2 fixing bacteria such as Caulobacter rhizosphaerae, Sphingomonas sp. and Burkholderia contaminans identified in the experimental soils may play an important role under P-deficient conditions.

Morphology of the nutrient artery and its foramen in relation to femoral bone perfusion rates of laying and non-laying hens.

If arteries penetrate bones through foramina, regional artery blood flow rates can be estimated from the foramen sizes. Femoral bone blood flow rates estimated from nutrient foramen sizes were previously not absolute, but only a relative blood flow index (Qi ), because the size relationship between the foramen and the occupying artery was unknown. The current study used vascular contrast and micro-computerized tomographic scanning to investigate femoral nutrient foramen and nutrient artery sizes in three groups of sub-adult chickens (non-laying hens, laying hens, and roosters) of similar ages. The results indicate that the cross-sectional area of the nutrient artery lumen occupies approximately 20.2±4.1% of the foramen for femora with only one foramen. Artery lumen size is significantly correlated with foramen size. Vascular contrast imaging is capable of estimating blood flow rates through nutrient arteries, as blood flow rates estimated from artery lumen casts are similar to blood flow rates measured by infusion of fluorescent-labeled microspheres. Laying hens tend to have higher nutrient artery perfusion rates than non-laying hens, probably due to extra oxygen and calcium requirements for eggshell production, although the calculated blood flow difference was not statistically significant. Histological embedding and sectioning along with vascular contrast imaging reveal variable nutrient foramen morphology and nutrient artery location among femora with more than one nutrient foramen.

Hatched and starved: Two chromatin compaction mechanisms join forces to silence germ cell genome.

Animals evolved in environments with variable nutrient availability and one form of adaptation is the delay of reproduction in food shortage conditions. Belew et al. (2021. J. Cell Biol.https://doi.org/10.1083/jcb.202009197) report that in the nematode C. elegans, starvation-induced transcriptional quiescence in germ cells is achieved through a pathway that combines two well-known chromatin compaction mechanisms.

Variations of heavy metals, nutrients, POPs and particle size distribution during “sludge anaerobic digestion-solar drying-land utilization process”: Case study in China

Sewage sludge was rich in pollutants such as heavy metals, nutrients and persistent organic pollutants. Anaerobic digestion can effectively degrade the pollutants while achieving sludge stabilization and reduction. This study took a sludge treatment plant located in Anhui Province, China as a case study, investigating the variations of multiple substances and particle size distribution during sludge anaerobic digestion-solar drying-land utilization process. The results demonstrated anaerobic digestion had a positive effect on the removal of heavy metals, nutrients and persistent organic pollutants, for polycyclic aromatic hydrocarbons, antibiotics and disinfection by-products with 41.38%, 62.26% and 68.68%, respectively, which were related to their molecular weight and structure. Large amounts of heavy metals would flow away with liquid digestate, in which Cr and Hg were the most and least, 90.44% and 41.95% respectively. The degradation of extracellular polymers in this process led to a decrease in particle size distribution, which caused the deterioration of sludge dewatering performance. Nutrients and water content increased and decreased respectively during solar drying along with the volatilization of organic matter under high temperature, which was beneficial for the final sludge product to subsequent land utilization. No significant correlation was demonstrated between heavy metals and sludge properties besides electrical conductivity. The study provided a new thinking on the variations of different substances and the way of actual treatment and disposal from the perspective of sludge anaerobic digestion-solar drying-land utilization process, which had a considerable significance for the further promotion and application of anaerobic digestion process in China's engineering community.

Assessing how changes in temporal resource quality influence the reproductive performance of the dung beetle Onthophagus binodis

1. Both larval and adult dung beetles depend on dung resources for nutrition. The quality of the dung resources utilised by beetles can influence adult morphology, adult biomass, and life history parameters of dung beetles. Although several studies have compared dung nutrients of different vertebrate dung types, variation in dung nutrients over time has been less studied.2. Here, moisture content and chemical parameters of dung were analysed at monthly intervals over 12 months. The reproductive output of Onthophagus binodis fed dung collected at four different months of the year is also measured to assess the relationship between dung resources and dung beetle performance. The experiments were carried out under controlled conditions: 25 ± 1 °C: 15 ± 1 °C (L: D) and 60 ± 5% RH, respectively, with a photoperiod of 14L:10D.3. Over the 12‐month sampling period, the maximum number of broods and highest brood weight were observed from beetles utilising dung collected in the Austral summer (February 2018). The analysis of dung over the 12‐month period showed that the electrical conductivity and phosphorous, manganese, and magnesium content of dung were highest in February 2018.4. The results suggest that the variation in dung resources, such as moisture and minerals, across the year affected the reproductive performance of Onthophagus binodis, which was highest when beetles were provisioned with summer dung.

Spatial variations and its influencing factors of soil carbon and nitrogen on the southern foot of Taihang Mountains, China

As an important ecological barrier for the North China Plain, research on the spatial variations of soil nutrients in the southern foot of the Taihang Mountains is of great significance for the forestry ecological construction in this rocky mountainous area. With the typical slopes (the artificial forestland and the natural wild slope) of the southern foot of the Taihang Mountains as the research objects, we used the grid method to arrange sampling points, and combined classical statistics, geostatistics and constrained sorting methods to analyze the spatial variations of soil nutrients. The results showed that: 1) soil total carbon (TC) contents were 6.80-57.05 g·kg-1, and the total nitrogen (TN) contents were 0.74-3.93 g·kg-1. The coefficients of variation of both soil TC and TN were 25.0%-52.8%, belonging to the moderate degree of variation, which were caused by the combination of random and structural factors. The spatial aggregation of soil nutrients decreased with increasing lag distances. 2) The contents of soil nutrients had increasing trends from the top to the bottom of the slopes, with high nutrient values appearing at the bottom of the slopes. 3) The soil bulk density, gravel content, vegetation coverage, and soil water content were the main factors affecting the spatial variability of soil TC and TN on the southern foot of the Taihang Mountains. 4) Soil water content was the main factor affecting soil nutrients at the natural wild slope, but not at the artificial forestland.

Comparing spiraling‐ and transport‐based approaches to estimate in‐stream nutrient uptake length from pulse additions

AbstractThe distance that a nutrient travels as a solute before its removal from the stream water column is known as the uptake length (SW), which is a functional indicator of environmental quality and integrity. Among nutrient enrichment methods, instantaneous nutrient addition (e.g., slug or pulse) have been proposed as an alternative to plateau and labeled nutrient approaches. Two approaches have been commonly used to estimate SW and its associated metrics (i.e., areal uptake rate, U; and uptake velocity, Vf) from pulse additions: the spiraling approach, based on the longitudinal variation in nutrient concentrations, and the transport modeling approach, based on the advective and dispersive transport of solutes. However, little is known in how the choice of such analytical methods impacts the estimation of stream uptake parameters and the conclusions we draw from them. Here, we estimated the SW and Vf of ammonium‐nitrogen (NH4‐N) and soluble reactive phosphorus (SRP) from 16 pulsed additions conducted in four low‐order streams in southeastern Brazil. We compared metrics estimated by the Tracer Additions for Spiraling Curve Characterization (TASCC) and the One‐Dimensional Transport with Inflow and Storage (OTIS) methods, based on the spiraling‐ and transport‐based approaches, respectively. The TASCC:OTIS SW ratio averaged 0.71 for NH4‐N and 1.01 for SRP, whereas the mean of TASCC:OTIS Vf ratio was 2.04 for NH4‐N and 1.03 for SRP. The results showed that both SW and Vf estimates differed significantly between methods for NH4‐N, but no statistical differences were observed in SRP estimates. In our study, we highlighted the significant effects of transient storage and variable nutrient concentration on pulsed enrichments. Such information should be considered when choosing which method is appropriate to use for a particular site. Differences between modeling approaches must be addressed when comparing methods to expand our knowledge on broad temporal and spatial patterns of in‐stream nutrient uptake.

Phytoplankton Bloom Dynamics in Incubated Natural Seawater: Predicting Bloom Magnitude and Timing

Phytoplankton blooms can cause imbalances in marine ecosystems leading to great economic losses in diverse industries. Better understanding and prediction of blooms one week in advance would help to prevent massive losses, especially in areas where aquaculture cages are concentrated. This study has aimed to develop a method to predict the magnitude and timing of phytoplankton blooms using nutrient and chlorophyll-a concentrations. We explored variations in nutrient and chlorophyll-a concentrations in incubated seawater collected from the coastal waters off Yeosu, South Korea, seven times between May and August 2019. Using the data from a total of seven bottle incubations, four different linear regressions for the magnitude of bloom peaks and four linear regressions for the timing were analyzed. To predict the bloom magnitude, the chlorophyll-a peak or peak-to-initial ratio was analyzed against the initial concentrations of NO3 or the ratio of the initial NO3 to chlorophyll-a. To predict the timing, the chlorophyll-a peak timing or the growth rate against the natural log of NO3 or the natural log of the ratio of the initial NO3 to chlorophyll-a was analyzed. These regressions were all significantly correlated. From these regressions, we developed the best-fit equations to predict the magnitude and timing of the bloom peak. The results from these equations led to the predicted bloom magnitude and timing values showing significant correlations with those of natural seawater in other regions. Therefore, this method can be applied to predict bloom magnitude and timing one week in advance and give aquaculture farmers time to harvest fish in cages early or move the cages to safer regions.