Stem Diameter Variations Research Articles

Stem Growth and Dehydration Responses of Mediterranean Tree Species to Atmospheric and Soil Drought.

Stem growth responses to soil and atmospheric drought are critical to forecasting the tree carbon sink strength. Yet, responses of drought-prone forests remain uncertain despite global aridification trends. Stem diameter variations at an hourly resolution were monitored in five Mediterranean tree species from a mesic and a xeric site for 6 and 12 years. Stem growth and dehydration responses to soil (REW) and atmospheric (VPD) drought were explored at different timescales. Annually, growth was determined by the number of growing days and hours. Seasonally, growth was bimodal (autumn growth ≈ 8%-18% of annual growth), varying among species and sites across the hydrometeorological space, while dehydration consistently responded to REW. Sub-daily, substantial growth occurred during daytime, with nighttime-to-daytime ratios ranging between 1.2 and 3.5 (Arbutus unedo ≈ Quercus faginea < Quercus ilex < Pinus halepensis in the mesic site, and Juniperus thurifera < P. halepensis in the xeric site). Overall, time windows favourable for growth were limited by soil (rather than atmospheric) drought, modulating annual and seasonal growth in Mediterranean species, and stems maintained non-negligible growth during daytime. These patterns contrast with observations from wetter or cooler biomes, demonstrating the growth plasticity of drought-prone species to more arid climate conditions.

Local neighborhood affects stem rehydration under drought: evidence from mixtures of European beech with two different conifers.

Mixed-species forests are, for multiple reasons, promising options for forest management in Central Europe. However, the extent to which interspecific competition affects tree hydrological processes is not clear. High-resolution dendrometers capture sub-daily variations in stem diameter; they can simultaneously monitor stem growth (irreversible changes in diameter) and water status (reversible changes) of individual trees. Using the information on water status, we aimed to assess potential effects of tree species mixture, expressed as local neighborhood identity, on night-time rehydration and water stress. We deployed 112 sensors in pure and mixed forest stands of European beech, Norway spruce, and Douglas fir on four sites in north-western Germany, measuring stem diameter in 10-minute intervals for a period of four years (2019-2022). In a mixture distribution model, we used environmental variables, namely soil matric potential, atmospheric vapor pressure deficit, temperature, precipitation, and neighborhood identity to explain night-time rehydration, measured as the daily minimum tree water deficit (TWDmin). TWDmin was used as a daily indicator of water stress and the daily occurrence of sufficient water supply, allowing for stem growth (potential growth). We found that species and neighborhood identity affected night-time rehydration, but the impacts varied depending on soil water availability. While there was no effect at high water availability, increasing drought revealed species-specific patterns. Beech improved night-time rehydration in mixture with Douglas fir, but not in mixture with spruce. Douglas fir however, only improved rehydration at a smaller share of beech in the neighborhood, while beech dominance tended to reverse this effect. Spruce was adversely affected when mixed with beech. At species level and under dry conditions, we found that night-time rehydration was reduced in all species, but beech had a greater capacity to rehydrate under high to moderate soil water availability than the conifers, even under high atmospheric water demand. Our study gives new insights into neighborhood effects on tree water status and highlights the importance of species-specific characteristics for tree-water relations in mixed-species forests. It shows that drought stress of European beech can be reduced by admixing Douglas fir, which may point towards a strategy to adapt beech stands to climate change.

Phenotypic diversity and population structure of Pecan (Carya illinoinensis) collections reveals geographic patterns

Pecan (Carya illinoinensis) is an economically important nut crop known for its genetic diversity and adaptability to various climates. Understanding the growth variability, phenological traits, and population structure of pecan populations is crucial for breeding programs and conservation. In this study, plant growth and phenological traits were evaluated over three consecutive seasons (2015–2017) for 550 genotypes from 26 provenances. Significant variations in plant height, stem diameter, and budbreak were observed among provenances, with Southern provenances exhibiting faster growth and earlier budbreak compared to Northern provenances. Population structure analysis using SNP markers revealed eight distinct subpopulations, reflecting genetic differentiation among provenances. Notably, Southern Mexico collections formed two separate clusters, while Western collections, such as 'Allen 3', 'Allen 4', and 'Riverside', were distinguished from others. 'Burkett' and 'Apache' were grouped together due to their shared maternal parentage. Principal component analysis and phylogenetic tree analysis further supported subpopulation differentiation. Genetic differentiation among the 26 populations was evident, with six clusters highly in agreement with the subpopulations identified by STRUCTURE and fastSTRUCTURE. Principal components analysis (PCA) revealed distinct groups, corresponding to subpopulations identified by genetic analysis. Discriminant analysis of PCA (DAPC) based on provenance origin further supported the genetic structure, with clear separation of provenances into distinct clusters. These findings provide valuable insights into the genetic diversity and growth patterns of pecan populations. Understanding the genetic basis of phenological traits and population structure is essential for selecting superior cultivars adapted to diverse environments. The identified subpopulations can guide breeding efforts to develop resilient rootstocks and contribute to the sustainable management of pecan genetic resources. Overall, this study enhances our understanding of pecan genetic diversity and informs conservation and breeding strategies for the long-term viability of pecan cultivation.

Variation in Phenology and Morphological Traits of Seed-Propagated Laggera alata and Laggera crispata Forms

The phenological and morphological variation studies among forms of Laggera Sch. Bip. Ex. Benth. and Hook species are limited, despite the medicinal use of the genus. Therefore, this study aimed to document phenology and morphological variation in cultivated populations of Laggera alata and Laggera crispata forms from seedling to maturity. The forms were categorized as Laggera alata with a small capitulum (LA-SC), Laggera alata with a large capitulum (LA-BC), Laggera crispata from South Africa (LC-SA), and Laggera crispata from Zimbabwe (LC-ZIM). Seeds were germinated in Petri dishes, transplanted to plug trays, and later to field plots at 60 days. Phenological events were recorded when observed in at least one plant. Twelve qualitative and four morphometric traits were measured monthly on five plants per Laggera form. Analysis of variance and Tukey’s Honestly Significant Difference test (p &lt; 0.05) were used for data analysis. Results indicated significant variation in phenology, qualitative traits, leaf traits, plant height, and stem diameter both within and between L. crispata and L. alata forms. Morphometric traits, such as leaf size and the number of leaves per plant, were identified as key descriptors for differentiating L. alata forms. These findings provide a foundation for the introduction of Laggera forms into farming systems for medicinal and commercial purposes.

Xylem Hydraulic Conductance Role in Kiwifruit Decline Syndrome Occurrence

Kiwifruit decline syndrome (KiDS) has affected kiwifruit orchards for more than ten years in the Mediterranean area, severely compromising productivity and causing extensive uprooting. The affected plants go through an irreversible and fast wilting process. The problem has not been solved yet, and a single cause has not been identified. In this work, we carried out a survey on ten five-year-old healthy kiwifruit cv. Hayward plants cultivated in an area strongly affected by KiDS and characterised by a rising temperature and vapor pressure deficit (VPD). Five plants were located in a KiDS-affected orchard. Our goal was to assess the hydraulic conductance of asymptomatic plants in a KiDS-affected area where rising climate change stress is underway. Our hypothesis was that a rising temperature and VPD could impair xylem functionality, leading the plants to develop strategies of tolerance, such as vessel narrowing, or stress symptoms, such as cavitation or implosion, inducing a higher risk of KiDS onset. Hydraulic conductance was investigated using a physiological and morphological approach to detect trunk sap flow, trunk growth and daily diameter variations, leaf gas exchanges and temperature, stem water potential, and the root xylem vessel diameter and vulnerability to cavitation. A strong xylem vessel narrowing was observed in all plants, with the highest frequency in the 30–45 µm diameter class, which is an indicator of long-term adaptation to a rising VPD. In some plants, cavitation and implosion were also observed, which are indicative of a short-term stress response; this behaviour was detected in the plants in the KiDS-affected orchard, where a high leaf temperature (&gt;39 °C), low stomatal conductance (&lt;0.20 mol H2O m−2 s−1) and transpiration (&lt;3 mmol H2O m−2 s−1), low stem water potential (&lt;−1 MPa), high vulnerability to cavitation (3.7 μm mm−2), low trunk sap flow and high daily stem diameter variation confirmed the water stress status. The concurrence of climate stress and agronomic management in predisposing conditions favourable to KiDS onset are discussed, evidencing the role of soil preparation, propagation material and previous crop.

Assessing intra-annual growth dynamics in climatically contrasting years, sites, and tree species using dendrometers and wood anatomical data

Detecting the intra-annual dynamics and courses of secondary tree growth enables the accurate identification of crucial steps in the forming of a new tree ring. Furthermore, comparing the high-resolution recordings of tree growth with environmental conditions allows assessment of the influence of weather on wood formation processes. This study investigates the intra-annual growth performance of conifer species and European beech at two high- and two low-elevation sites in Bavaria, southeast Germany. We measured stem circumference changes with electronic band dendrometers and cambial dynamics by collecting microcores at biweekly intervals. We analyzed growth variations between the consecutive years 2020 and 2021, which showed distinct climatic differences during the growing seasons. While warm and dry conditions prevailed in spring and summer in 2020, spring in 2021 was comparatively cold, and summer precipitation was high. Different tree growth patterns were observed in the contrasting years 2020 and 2021. Distinct growth reductions occurred in the drier year 2020 for most of the studied tree species, while trees showed wider tree rings in 2021 despite of low growth rates at the beginning of the growing season. Climate-growth correlations exposed the intraseasonal influence of climatic conditions, particularly available soil water, water vapor pressure deficit, and soil temperature, on short-term tree responses. Wood anatomical analysis and daily stem diameter variations proved to be valid monitoring methods to assess individual wood formation processes and to identify species-specific tree responses to the influence of climatic conditions. However, combining both methods represents the most reliable approach due to the mutual ability to compensate for each other’s deficiencies. While dendrometers provided a very accurate and high-resolution record of intra-annual tree growth, wood anatomical analyses were more reliable in determining the exact onset and cessation of wood formation. For this reason, combining both is recommended for assessing prospective tree growth performance in the context of climate change.

Unpacking the point of no return under drought in poplar: insight from stem diameter variation.

A specific, robust threshold for drought-induced tree mortality is needed to improve the prediction of forest dieback. Here, we tested the relevance of continuous measurements of stem diameter variations for identifying such a threshold, their relationship with hydraulic and cellular damage mechanisms, and the influence of growth conditions on these relationships. Poplar saplings were grown under well-watered, water-limited, or light-limited conditions and then submitted to a drought followed by rewatering. Stem diameter was continuously measured to investigate two parameters: the percentage loss of diameter (PLD) and the percentage of diameter recovery (DR) following rewatering. Water potentials, stomatal conductance, embolism, and electrolyte leakage were also measured, and light microscopy allowed investigating cell collapse induced by drought. The water release observed through loss of diameter occurred throughout the drought, regardless of growth conditions. Poplars did not recover from drought when PLD reached a threshold and this differed according to growth conditions but remained linked to cell resistance to damage and collapse. Our findings shed new light on the mechanisms of drought-induced tree mortality and indicate that PLD could be a relevant indicator of drought-induced tree mortality, regardless of the growth conditions.

Effects of fertilization on radial growth of Pinus pinea explored hourly using dendrometers

BackgroundStone pine (Pinus pinea), a drought-resistant species, has significant socio-economic benefits and increasing interest for the establishment of productive plantations in several countries, especially in a climate change context. Monitoring hourly stem diameter variations contributes to the understanding of the tree-growth response to changes in environmental conditions and management. By monitoring the diameter expansion of tree stems, high-resolution band dendrometers were used to study the development of adult trees growing in a semi-arid coastal environment of central Chile under fertilized and non-fertilized soil conditions through the span of a year.ResultsShort cycles (< 21 h) were few in fertilized and non-fertilized trees (6 and 4, respectively), whereas long cycles (> 28 h) occurred at a higher frequency in fertilized trees (16 vs 6). Most of the circadian cycles were regular (24 ± 3 h). The longest cycle duration (59 h) was observed in fertilized trees during spring. In all seasons, each phase of the circadian cycle, especially during the stem diameter increment phase (P3, irreversible growth), started earlier in fertilized than in control trees. P3 duration was significantly longer in fertilized than in control trees in springtime. The maximum shrinkage (P1) was observed in summer for both treatments. Stem diameter increased faster in fertilized than in control trees throughout the year, with the highest accumulation occurring in spring and the lowest in autumn. The daily variability pattern showed lower growth under high temperature across seasons.ConclusionsThis study highlights the importance of fertilization in enhancing stone pine diameter growth. This cultural practice should be further explored to contribute to the mitigation of climate change effects in semi-arid environments.

Comparison of Xylem Anatomy and Hydraulic Properties in Black Locust Trees at Two Growth Stages in Semiarid China

Tree species transitioning between different developmental phases requires homeostatic adjustments in order to maintain the integrity of the tree hydraulic system. Hence, adjustments related to hydraulic traits (e.g., xylem conduit diameter) are of key functional significance. However, critical information on the differences between different developmental stages is rare. Using sapwood samples from 36 black locust trees with different growth stages (actively growing and declining stages) and a soil water gradient along a hillslope, xylem conduits at stem apexes and breast height (1.3 m above ground) stems were measured. The results showed marked differences in vascular traits between actively growing and declining trees. In contrast to actively growing trees, declining trees exhibited a reduction in conduit diameters accompanied by increased frequency with a positively skewed distribution and a subsequent decline in cumulative theoretical hydraulic conductivity. Across all sampled trees, the hydraulically weighted mean conduit diameter tapered acropetally from breast height to the stem apex. The extent of conduit tapering in actively growing trees (0.244, 95% CI 0.201–0.287) aligned with predictions from the hydraulic optimality model. Conversely, trees in a declining status displayed significantly reduced conduit tapering (0.175, 95% CI 0.146–0.198), indicating an elevation in hydraulic resistance with increasing tree height. Variations in hydraulic properties predominantly resulted from differences in tree height rather than variations in stem diameter or soil water content. The correlation between conduit diameter and soil water content in both actively growing and declining trees stemmed indirectly from variations in tree height rather than presenting a direct response to drought stress.

Impacts of site aridity on intra-annual radial variation of two alpine coniferous species in cold and dry ecosystems

Climate change alters regional aridity and species composition in dryland ecosystems. However, the effect of varying aridity and species-specific responses on tree growth remains unclear. Here, we used high-resolution dendrometers to analyze the climatic factors controlling stem diameter variation in two alpine coniferous species, Qilian juniper (Juniperus przewalskii) and Qinghai spruce (Picea crassifolia), in their eastern- and western-most distributions (two species × two sites) in the Qilian Mountains during the 2018–2021 growing seasons. We observed species-, site-, and year-specific differences in growth phenology and radial growth dynamics. Juniper had early growth onset and cessation, but short growing season compared to spruce. Trees in the eastern sites showed earlier growth onset, later cessation, and a much longer growing season than those in the western sites for both species. Daily stem growth revealed similar responses for both species and site aridity, with daily vapor pressure deficit, maximum temperature, and solar radiation negatively, and relative humidity and precipitation positively affecting stem increments. However, when observation was extended to 7 or 21 days, the effect of precipitation on stem increments became weak or negligible in the wetter eastern sites, while continuing in the arid western sites. Further, soil water content appeared to have an impact on growth in the arid western site for both species. These results were confirmed by linear mixed-effects models, which revealed that site-aridity play an important role on tree stem variation, especially in junipers. Our results suggest that although short-term atmospheric water conditions influence radial stem variation, intra-annual tree growth still depends on soil water availability in drylands. Our findings provide new evidence of potential uses of dendrometers to measure environmental stress on tree growth and reveal that site aridity and species influence tree growth at different time scales in cold and arid ecosystems.

Mechanisms of grapevine resilience to a vascular disease: investigating stem radial growth, xylem development and physiological acclimation.

Plant vascular diseases significantly impact crop yield worldwide. Esca is a vascular disease of grapevine found globally in vineyards which causes a loss of hydraulic conductance due to the occlusion of xylem vessels by tyloses. However, the integrated response of plant radial growth and physiology in maintaining xylem integrity in grapevine expressing esca symptoms remains poorly understood. We investigated the interplay between variation in stem diameter, xylem anatomy, plant physiological response and hydraulic traits in two widespread esca-susceptible cultivars, 'Sauvignon blanc' and 'Cabernet Sauvignon'. We used an original experimental design using naturally infected mature vines which were uprooted and transplanted into pots allowing for their study in a mini-lysimeter glasshouse phenotyping platform. Esca significantly altered the timing and sequence of stem growth periods in both cultivars, particularly the shrinkage phase following radial expansion. Symptomatic plants had a significantly higher density of occluded vessels and lower leaf and whole-plant gas exchange. Esca-symptomatic vines showed compensation mechanisms, producing numerous small functional xylem vessels later in development suggesting a maintenance of stem vascular cambium activity. Stabilization or late recovery of whole-plant stomatal conductance coincided with new healthy shoots at the top of the plant after esca symptoms plateaued. Modified cropping practices, such as avoiding late-season topping, may enhance resilience in esca-symptomatic plants. These results highlight that integrating dendrometers, xylem anatomy and gas exchange provides insights into vascular pathogenesis and its effects on plant physiology.

Daily and seasonal patterns of stem diameter micro-variations in three semiarid forest species in relation to water status and environmental factors

Understanding the relationship between stem diameter micro-variation (SDMV) and stem water status and revealing the mechanism regulating stem water are essential for elucidating environmental adaptation and water use strategies in forest trees. Here, we report the results of the year-round monitoring (November 2018–October 2019) of SDMV and xylem sap flow obtained using high-resolution dendrometers and Granier-type thermal dissipation probes, respectively, in mature trees of three species in the semiarid Loess Plateau region: Quercus liaotungensis, Platycladus orientalis, and Robinia pseudoacacia. The year-round trend for the variations in stem diameter can be divided into phases of a decreasing period and a relatively stable period in the non-growing season, and a progressive rise and fluctuating plateau throughout the growing season. The observed interspecific differences in the time to full recovery from the water deficit state corresponded to species phenology and soil moisture conditions. These differences were manifested as the responses of sap flux density (Fd) and tree water deficit (TWD) to variations in soil water content during the growing season. Q. liaotungensis implements stomatal regulation early to avoid drastic water loss in the trunk, P. orientalis maintains a relatively stable Fd at the expense of increased TWD under soil drought, and R. pseudoacacia maintains relatively low stomatal conductance under soil drought with an increase in TWD. The results revealed that SDMV is determined by the stem water status and species-specific strategies for water use, which could provide beneficial information for forestation practices in semiarid regions.

Effect of NPK fertiliser on upper and basal stem diameters and implication on growth habit of tomato

Prostrate growth caused by weak stems and variations in upper and basal stem diameters (USD and BSD, respectively) is inherent in tomato (Solanum lycopersicum L.) and can be aggravated by increased nitrogen fertiliser. Application of a nitrogen fertiliser rate that minimises variation in stem diameters and increases yield can contribute to improved tomato production. The tomato cvs. Buffalo, Cobra, Kerewa, Roma-Savanna, Roma-VF, Tropimech, and UC-82, were grown at Ilara and Imeko with 0, 30, 50 or 80 kg/ha of 15 N-15P-15 K fertiliser. Application of 15 N-15P-15 K increased stem diameter variation. Tomato cultivated in Ilara produced higher number of fruits and fruit weight/plant than those in Imeko but maintained similar habit (40.5° and 42.3°, respectively) in both locations. ‘Buffalo’ and ‘Roma-VF’ had 42.5° and 22.5° habits and fruit weights (84.4 and 103 g) above average under low (30 kg/ha) and high (80 kg/ha) rates of 15 N-15P-15 K, respectively; ‘Cobra’ combined erect growth (36.3° and 41.2°) and yield above average (107.4 and 101.5 g) under 30 and 80 kg/ha, respectively across locations. Out of 672 plants sampled, 67.4% had USD > BSD, 18.6% USD < BSD, and 14% had USD = BSD. With increased rates of 15 N-15P-15 K fertiliser up to 80 kg/ha, the percent of plants with USD > BSD increased from 49.4 to 76.2% and increased prostrate angle from 26.4° to 48.4°. Higher yield combined with erect growth above average can be achieved with 30 kg/ha in ‘Buffalo’ and ‘Cobra’ across the locations. Application of lower rates of 15 N-15P-15 K fertiliser can help in balancing the trade-off between erect growth and higher fruit yield of tomato.

A method to quantify and account for the hygroscopic effect in stem diameter variations

Dendrometers recording stem diameter variations (SDV) at high-resolution are useful to assess trees' water relation since water reserves are stored in the elastic tissue of the bark. These tissues typically shrink during the day as they release water when evaporative demand is high and swell during the night as they are replenished when evaporative demand is low, generating the typical SDV profile known as the diel SDV cycle. However, similar SDV cycles have been observed on dead trees due to the hygroscopic shrinking and swelling of the dead bark tissues. In order to remove this hygroscopic effect of the bark, dendrometers are applied as close as possible to the living bark tissues by removing the outer dead layer, however with questionable success. In this study, we used SDV time series from 40 point dendrometers applied on dead-bark-removed mature trees to assess and quantify the remaining hygroscopic effect on individual trees. To do so, we checked SDV behavior in the cold season and explored the relation between the diel SDV cycle and changes in relative humidity (RH). Our results showed that (a) the hygroscopic effect in SDV can be well-detected based on the amplitude of the diel SDV cycle (diel SDVampl) and the correlation between SDV and RH during both the cold and the warm season; (b) the level of the hygroscopic effect varies strongly among individuals; (c) diel SDVampl is proportional to both changes in RH and transpiration so that the hygroscopic effect on the diel SDV cycle can be quantified using a linear model where (diel SDVampl) is a function of RH changes and transpiration. These results allow the use of the model to correct the amplitude of the diel SDV cycles and suggest that this method can be applied to other ecological relevant water-related SDV variables such as tree water deficit.

Residual Effect of Sago Dregs Fertilizer to Okra (Abelmoschus esculentus) Growth and Yield in Planting Period II in Marginal Land

Aims: The research aimed to study the potential of sago dregs fertilizer residue in increasing the growth and yield of okra (Abelmoschus esculentus) in planting period II on marginal land.&#x0D; Study Design: The research was conducted using a randomized block design with a single factor.&#x0D; Place and Duration of Study: The research was conducted in Field Laboratory of Faculty of Agriculture, University of Halu Oleo, Kendari, Southeast Sulawesi, Indonesia. The study was conducted for four months.&#x0D; Methodology: Single factor design in Randomized block design was used in the research. consists of 6 treatment levels of sago dregs fertilizer residue that have been applied in the previous planting period (planting period I), namely: without sago dregs (S0), using sago dregs 5 t ha-1 (S1), 10 t ha-1 (S2), 15 t ha-1 (S3), 20 t ha-1 (S4) and 25 t ha-1 (S5). Each treatment was placed in three groups so that there were 18 experimental units. The data was analyzed using analysis of variance and continued by Tukey Test 95% confidence level.&#x0D; Results: The results generally showed that the sago dregs fertilizer residual had a significant effect on the growth and yield of Abelmoschus esculentus. The effectiveness of the residual effect of sago pulp organic fertilizer is 3.39% to 25.68% on the variable plant height, 12.77% to 24.07% on the variable stem diameter, 17.72% to 32.26% on the variable number of leaves, 23.84% to 53.03% on the variable area leaves, 24.06% to 39.15% in plant dry weight variable, 20.21% to 41.61% on the variable number of fruit and 14.46% to 34.21% on the variable weight of fruit. Strongly influenced by the dose of sago pulp organic fertilizer applied in growing season I.&#x0D; Conclusion: Residual of sago dregs fertilizer potentially increasing of okra (Abelmoschus esculentus) growth and yield in planting period II in marginal land. The effectiveness highest was achieved in the treatment of 15 t ha-1 sago dregs organic fertilizer, for all of variables observed.

Interspecific Differences of Stem Diameter Variations in Response to Water Conditions for Six Tree Species in Northeast China

It is important to understand the response of stem diameter variations in dominant tree species to water conditions in Northeast China. The results will provide basic information for scientific predictions of the future development trend of temperate forests in the eastern mountainous area of northeast China. We employed a high-precision dendrometer to continuously monitor the stem radial changes of six dominant tree species in temperate forests in northeast China from 15 April to 24 October in 2021. Precipitation significantly promoted the tree stem diameter increment. The increment of stem diameter in Juglans mandshurica Maxim., Quercus mongolica Fisch. and Betula platyphylla Suk. had a significantly positive correlation with cumulative precipitation. Correlation analysis revealed that the stem radial change (SRC) of six tree species was positively correlated with precipitation (Pre) and relative humidity (RH), and negatively correlated with water vapor pressure deficit (VPD), indicating that the diameter growth of the six tree species was mainly restricted by water conditions. Under drought stress, the stem radial growth rate of the six tree species slowed down, the growth duration decreased and the tree water deficit (TWD) value increased, while there were obvious interspecific differences. Therefore, water conditions limited the stem radial growth of the six tree species, while each tree species had a different response to drought stress.

Stem Diameter Decrement in Holm Oak (Quercus rotundifolia Lam.): Insights into Tree Decline Pathways in Endangered Woodlands of Southern Portugal

Stem diameter growth in living trees refers to the invariably increase in dimension over a given period. However, reversible stem diameter decrease could occur, related to water movement in the vessels within the wood (on a daily basis) and to tree water deficit and depletion of stored water (on a seasonal basis). Recently, the perennial decrement in stem diameter size has been related to a tree decline pathway, and eventually resulting in tree death. In this study, we analysed stem diameter intra-annual growth dynamics of holm oak (Quercus rotundifolia Lam.) trees for two full growing seasons in distinct woodlands, Laborela and Aldeia dos Fernandes, in southern Portugal (Ourique district). Our focus was to assess stem diameter growth patterns and trends in holm oak trees in endangered woodlands with high tree mortality and to question if perennial decrement. Holm oaks in Laborela were much more sensitive in their stem diameter variations than in Aldeia dos Fernandes and, on average, their stem diameter decreased continuously by 1.0 mm along the 2-year study period, with a slightly higher annual decrease in the first study year (0.6 mm.yr−1). In addition, on average, trees had a higher decrease in stem sectional area of 5.8 cm2 in Laborela against a decrease of 3.7 cm2 in Aldeia dos Fernandes, where the stem diameter of holm oaks only decreased in the first study year (0.1 mm.yr−1). In each study area, the repeated- measures ANOVA showed that tree size effect influenced the stem diameter variations in contrast to tree crown defoliation. Trees were, on average, relatively smaller in Laborela, with DBH = 35.5 cm against DBH = 40.6 cm in Aldeia dos Fernandes, and were highly sensitive in their stem diameter decrement along two consecutives full growing seasons. This is the first exploratory study on assessing the stem diameter fluctuations on holm oaks to address a decline pathway. Our results revealed that holm oaks can survive during two consecutive growing seasons, with a continuous decrease in their stem diameters, which might indicate one tree’s decline pathway.

Postharvest illumination of alstroemeria: Effect of light quality on flower metabolism and shelf life

Despite the good postharvest performance of alstroemeria flowers, leaf yellowing is one of the most important signs of senescence and decay, as well as the flower size and time to abscission. In this work, we tested the effect of 50 μmol m−2 s−1 white (WL, broad spectrum) and red-blue (RBL, 40:60, 630 + 465 nm) light-emitting diodes on cut alstroemeria stored at 20 °C, or WL on cut alstroemeria stored at 5 °C, on postharvest flower quality and vase life. The light-treated floral stems had higher water consumption throughout storage compared to the controls stored in the dark, with no variation in stem diameter. The tepal area was increased by the light treatment at both storage temperatures. The leaf color remained similar to that at harvest in the light-treated flower stems (WL and RBL), while there was a strong yellowing in the controls stored in the dark. Anthocyanins increased mostly in flowers stored under WL, although this was more evident at 20 °C. WL treatment strongly slowed chlorophyll degradation and increased sugar content in leaves and tepals by 3.4 and 1.8 times, respectively, at 20 °C, and by 6 and 2.9 times, respectively, at 5 °C. The increase in sugar levels in light-treated flowers may have caused the tepal expansion but also delayed the onset of leaf senescence, and consequently the tepal abscission. The WL was the most effective storage condition not only to extend cut alstroemeria shelf life, but also to improve the postharvest flower quality, both at room temperature and under refrigeration.

Time Lag of Stem Water Deficit in Response to Increased Vapor Pressure Deficit

Abstract Vapor pressure deficit (VPD) shows a gradually increasing trend that forces trees to produce ecological memory with time-delay characteristics to drought year by year. The reversible stem diameter variation of trees can serve as an indicator for tree water status. In the context of global warming, these variations can dynamically monitor the response of stem water to the change of environmental water, especially to VPD. In this study, stem diameter variations of Larix gmelinii Rupr. and environmental factors were measured at high temporal resolution. We analyzed the tree water deficit–induced stem shrinkage (TWD) response to VPD variations. The results showed that variation of TWD lagged behind that of VPD to a certain extent, by about 3–5.5 h. Environmental water supply of trees under natural conditions can be divided into water deficit and adequate water supply. The division of the two water conditions was helpful to study the correlation between TWD and VPD. Observing the response of changes of stem diameter to the time lag of VPD is helpful to accurately represent the relationship of changes between moisture in the tree body and moisture in the air and to identify the stress effect of the lag in a short period of time. Study Implications: Stem diameter changes with change in moisture in the environment and usually reversibly shrinks under water deficits. Such reversible change in stem diameter is closely related to change in vapor pressure deficit, which is an environmental factor. Tree water deficit–induced trunk shrinkage, an index reflecting the change in stem diameter affected by moisture, had a time lag correlation with vapor pressure deficit in terms of variation characteristics. The time lag of the tree water deficit–induced trunk shrinkage response to vapor pressure deficit was 3–5.5 h. Just as the phenomenon of ecological memory cannot be ignored in an observational study with the year as the time scale, the phenomenon of time lag should not be ignored during an observational study of moisture stress at small time scales.

Konsentrasi Eco Enzyme berbasis Sumber Daya Lokal Ternate Mendukung Pertumbuhan Tanaman Kailan (Brassica oleracea var. achepala)

This study aims to examine the concentration of Eco-enzymes based on local Ternate resources in dealing with the greed of Tutman Kailan which was carried out in the Tabam sub-district, North Ternate district. The study used a factorial completely randomized completely randomized design (CRD) with two factors as the main factors. Concentration of top taluk ecoenzyme Eight concentrations of top taluk taluk without concentration (R0) to 7 ml/l water (R7) and lead factor. Kailan seed variety that talukat consists of two varieties, namely the Daily Fram variety and the red arrow variety. The tools used in this study were digital scales, ruler, caliper, spoon, 5 kg polybag, digital camera, sack, label, stationery, paper, hoe. The materials used in the research were soil media, kailan seed, Eco-enzyme. The research was carried out in several stages, namely the manufacture of eco enzymes, preparation of planting media, planting, and data collection. The results showed that the concentration of eco-enzyme based on local resources in Ternate did not have an interaction effect but gave a singular effect, a concentration of 5 ml/l water (R5) resulted in the highest plant height of 34 cm and the number of leaves 7.67 while in leaf area, 2006, 85 cm at a concentration of (R7) 7ml/water but not different from R5. Variable stem diameter Plant fresh weight and root dry weight had no significant effect, while the total fresh weight of treatment R5 gave the highest fresh weight of 280 g in the red arrow variety (V2) as well as the total dry weight of 23.37 g in treatment R5 but the difference variety has no effect.Keywords: Concentration of eco enzyme, local resources, kailan plant