Variable Length Research Articles

ARGNet: using deep neural networks for robust identification and classification of antibiotic resistance genes from sequences

BackgroundEmergence of antibiotic resistance in bacteria is an important threat to global health. Antibiotic resistance genes (ARGs) are some of the key components to define bacterial resistance and their spread in different environments. Identification of ARGs, particularly from high-throughput sequencing data of the specimens, is the state-of-the-art method for comprehensively monitoring their spread and evolution. Current computational methods to identify ARGs mainly rely on alignment-based sequence similarities with known ARGs. Such approaches are limited by choice of reference databases and may potentially miss novel ARGs. The similarity thresholds are usually simple and could not accommodate variations across different gene families and regions. It is also difficult to scale up when sequence data are increasing.ResultsIn this study, we developed ARGNet, a deep neural network that incorporates an unsupervised learning autoencoder model to identify ARGs and a multiclass classification convolutional neural network to classify ARGs that do not depend on sequence alignment. This approach enables a more efficient discovery of both known and novel ARGs. ARGNet accepts both amino acid and nucleotide sequences of variable lengths, from partial (30–50 aa; 100–150 nt) sequences to full-length protein or genes, allowing its application in both target sequencing and metagenomic sequencing. Our performance evaluation showed that ARGNet outperformed other deep learning models including DeepARG and HMD-ARG in most of the application scenarios especially quasi-negative test and the analysis of prediction consistency with phylogenetic tree. ARGNet has a reduced inference runtime by up to 57% relative to DeepARG.ConclusionsARGNet is flexible, efficient, and accurate at predicting a broad range of ARGs from the sequencing data. ARGNet is freely available at https://github.com/id-bioinfo/ARGNet, with an online service provided at https://ARGNet.hku.hk.7nR91jX7ySRMRPZrE5tsTyVideo

Elastic solids under frictionless rigid contact and configurational force

A homogeneous elastic solid, bounded by a flat surface in its unstressed configuration, undergoes a finite strain when in frictionless contact against a rigid and rectilinear constraint, ending with a rounded or sharp corner, in a two-dimensional formulation. With a strong analogy to fracture mechanics, it is shown that (i.) a path-independent J–integral can be defined for frictionless contact problems, (ii.) which is equal to the energy release rate G associated with an infinitesimal growth in the size of the frictionless constraint, and thus gives the value of the configurational force component along the sliding direction. Furthermore, it is found that (iii.) such a configurational sliding force is the Newtonian force component exerted by the elastic solid on the constraint at the frictionless contact. Assuming the kinematics of an Euler–Bernoulli rod for an elastic body of rectangular shape, the results (i.)–(iii.) lead to a new interpretation from a nonlinear solid mechanics perspective of the configurational forces recently disclosed for one-dimensional structures of variable length. Finally, approximate but closed-form solutions (validated with finite element simulations) are exploited to provide further insight into the effect of configurational forces. In particular, two applications are presented which show that a transverse compression can lead to Eulerian buckling or to longitudinal dynamic motion, both realizing novel examples of soft actuation mechanisms. As an application to biology, our results may provide a mechanical explanation for the observed phenomenon of negative durotaxis, where cells migrate from stiffer to softer environments.

Polymer optical fiber amplifier based on all-inorganic perovskite quantum dots

The proliferation of polymer optical fibers (POFs) has opened multiple avenues of optical-based networks and sensing applications in the visible spectral range. However, the lack of efficient amplifiers significantly hinders their utilization in practical scenarios. As emerging gain media, halide perovskites have attracted considerable attention in exploring their practical applications. In this Letter, we investigated the optical gain properties of cesium lead bromide quantum dots (CsPbBr3 QDs), and by facet dip-coating, we realized a polymer optical fiber amplifier. Under a 400-nm, 163-fs Ti:Sapphire laser pumping, the amplified spontaneous emission (ASE) thresholds of 1.6 and 20.1 μJ/cm2 were achieved through stripe pumping of a QDs-thin-film and end-pumping of a polymer-fiber facet dip-coated with the QDs, respectively. A gain coefficient of 232.2 ± 22.8 cm−1 was obtained using the variable stripe length method. By coupling a broadband continuous-wave light source into the POF as the signal, the optical gain behavior was studied with varying pump fluence and signal power density. More than 20 dB optical gain was achieved within the ASE wavelength region of 530–540 nm with a predicted theoretical maximum gain of 33.6 dB. The research verifies the feasibility of amplifying continuous-wave signals in the visible spectrum and potentially closing the research gap in visible-light optical-to-optical amplifiers. This opens the avenue for further research and innovations in practical polymer-based optical amplification for a plethora of applications, including all optical processing chips and short-range interconnects, as well as visible-light and underwater communications.

Hepatitis B virus particles in serum contain minus strand DNA and degraded pregenomic RNA of variable and inverse lengths.

This study utilized digital PCR to quantify HBV RNA and HBV DNA within three regions of the HBV genome. Analysis of 75 serum samples from patients with chronic infection showed that HBV RNA levels were higher in core than in S and X regions (median 7.20 vs. 6.80 and 6.58 log copies/mL; p < .0001), whereas HBV DNA levels showed an inverse gradient (7.71 vs. 7.73 and 7.77 log copies/mL, p < .001). On average 80% of the nucleic acid was DNA by quantification in core. The core DNA/RNA ratio was associated with viral load and genotype. In individual patients, the relations between RNA levels in core, S and X were stable over time (n = 29; p = .006). The results suggest that pregenomic RNA is completely reverse transcribed to minus DNA in ≈75% of the virus particles, whereas the remaining 25% contain both RNA and DNA of lengths that reflect variable progress of the polymerase.

The onset of instability and bifurcations in the transitional wake of two tandem square cylinders

Unsteady three-dimensional simulations are performed to elucidate the hidden transitional flow dynamics and Hopf bifurcations along the topological corelines of the created von Kármán streets behind a pair of square cylinders positioned in tandem. Our simulations provide significant new insight into the three-dimensional wake evolution and governing flow physics. We explain how pressure, velocity, and vorticity fluctuation along the Kármán vortex corelines in the increasingly unstable wake amplify, facilitating the growth of various modal instability patterns. The existing knowledge of wake transition through the intertwining of modes A, B, and C instabilities and associated linear stability analysis helped to gain some insight into the overall wake feature. The current study explains how exactly the transitional disturbances physically spread behind a pair of inline and tandem cylinders through the self-excited spanwise-periodic oscillation of the wake and created a sequence of variable length scaled Hopf bifurcations and their swapping for varied gaps between the cylinders and Reynolds number. The growth of a slow mode of the spectral frequency at the bifurcation points seemed crucial in initiating the near-transitional flow irregularity.

An electromechanically driven dielectric elastomer based tunable reflector

Deformable optics offer numerous advantages over conventional optical assemblies, including compactness, cost-effectiveness, efficiency, and flexibility. This study focuses on a reflector based on dielectric elastomer actuators (DEAs) with an internal fluid (air) coupling. DEAs are a class of electroactive materials adept at accommodating substantial actuation strains and rapid responses. Fluid distributed between the active and passive parts remains entirely enclosed by the device and transmits actuation pneumatically. Dynamic maneuvers conducted through a series of controlled electrical signals demonstrate proper control over optical characteristics. However, DEs exhibit inherent flaws in dynamic actuation, referred to as instabilities, which are mitigated by applying an initial pre-stretch. The study identifies optimal parameters that confer stability to the reflector: minimum to no creep, zero residual vibrations, and low viscous losses. An analytical framework is developed to assess device performance, focusing on the spherical curvature assumption that closely resembles the behavior of tunable spherical reflectors. Additionally, an optical bench setup is employed to demonstrate the relationship between focal length and applied pressure. Notably, this paper underscores the potential of a DE-based variable focal length reflector to function effectively within a dynamic environment.

Determining the mechanism for calculating the tension of a working conveyor belt during a change in the transportation length

This paper examines the working process of a belt conveyor with a working drive that can change the length of transportation. The conveyor can be used for tunneling, development of minerals in mines and quarries, transportation of materials in warehouses. The use of such a conveyor makes it possible to reduce the time for operations to increase or decrease the length of transportation, to exclude reloaders between the working equipment and conveyor itself from the transport chain. It was established that when changing the length of transportation of a working conveyor, the static and dynamic load on the belt increases. The change in the static load of the belt on the drum of the mobile station depends on the speed of the mobile station and the speed of the beltgenerated by the conveyor drive. The dynamic loading on the belt depends on the acceleration of the belt, which is related to the acceleration of the mobile station during the change in the length of the conveyor. For a working conveyor that changes the length of transportation, the static tension of the belt on the drum of the mobile station can increase by 1.1–1.4 times from the initial one. The dynamic loading of the belt can have a significant increase if the acceleration of the mobile station is not stretched over time and acquires large values. Based on the dependencesderived in the current work, a calculation procedure is proposed for the design of a belt conveyor with a working drive that can change the length of transportation. The Mathcad software was applied to verify the designcalculation procedure. The results make it possible to employ new design methods in the construction of competitive machines equipped with a belt conveyor with a variable length of transportation

First record of males of &lt;i&gt;Cypridopsis vidua&lt;/i&gt; (Crustacea, Ostracoda) species complex for Europe

Until recently, only asexually reproducing populations were known in Cypridopsis vidua, a species complex with one of the widest geographical distributions among non-marine ostracods. The discovery of males, most likely functional, from the United States and China has called into question its obligate parthenogen status. Here we report the first report of males for Europe, found in a small artificial pond in northern Italy. It seems plausible that sexual populations are much more widespread than hitherto known, as the recognition of males requires the dissection of individuals. Compared with the available descriptions of males belonging to Cypridopsis vidua and related species, that of the male illustrated here show morphological differences in the antennulae and prehensile palps, which in the Cypridoidea are sexually dimorphic and often diagnostic to distinguish between related species. Adult males show significantly smaller valve sizes than adult females, while adult females in which sperm were found inside the carapace have more variable lengths than those without sperm. No differences in valve coloration or dark banding pattern were found between males and females, and differences in valve outline were mostly negligible. The limitations of an exclusively morphological approach in the case of similar morphospecies are highlighted, as well as the importance of adopting objective criteria to measure the limits of morphological variability allowing the identification of separate taxonomic entities.

Ernica Clinical Consensus Statements on Total Colonic and Intestinal Aganglionosis

BackgroundHirschsprung disease is a congenital intestinal motility disorder characterized by an absence of enteric ganglion cells. Total colonic aganglionosis and near total or total intestinal aganglionosis, defined as absence of ganglion cells in the entire colon and with variable length of small bowel involved, are life-threatening conditions which affect less than 10 % of all patients with Hirschsprung disease. The aim of this project was to develop clinical consensus statements within ERNICA, the European Reference Network for rare congenital digestive diseases, on four major topics: Surgical treatment of total colonic aganglionosis, surgical treatment of total intestinal aganglionosis, management of poor bowel function in total colonic and/or intestinal aganglionosis and long-term management in total colonic and or intestinal aganglionosis. MethodsA multidisciplinary panel of representatives from ERNICA centers was invited to participate. Literature was searched, using specified search terms, in Medline (ALL), Embase and Google Scholar. Abstracts were screened and full text publications were selected. The panel was divided in four groups that extracted data from the full text publications and suggested draft statements for each of the major topics. A modified Delphi process was used to refine and agree on the statements. ResultsThe consensus statement was conducted by a multidisciplinary panel of 24 participants from 10 European countries, 45 statements reached consensus after 3 Delphi-rounds. The availability of high-quality clinical evidence was limited, and most statements were based on expert opinion. Another 25 statements did not reach consensus. ConclusionsTotal colonic and total intestinal aganglionosis are rare variants of Hirschsprung disease, with very limited availability of high-quality clinical evidence. This consensus statement provides statements on the surgical treatment, management of poor bowel function and long-term management for these rare patients. The expert panel agreed that patients benefit from multidisciplinary and personalized care, preferably in an expert center. Type of StudyClinical consensus statement. Level of Evidence3a.

Functionalization of 2-Mercapto-5-methyl-1,3,4-thiadiazole: 2-(ω-Haloalkylthio) Thiadiazoles vs. Symmetrical Bis-Thiadiazoles.

A study on the functionalisation of 2-mercapto-5-methyl-1,3,4-thiadiazole has been conducted, yielding two series of products: 2-(ω-haloalkylthio)thiadiazoles and symmetrical bis-thiadiazoles, with variable chain lengths. The experimental conditions were optimised for each class of compounds by altering the base used and the reagents' proportions, leading to the development of separate protocols tailored to their specific reactivity and purification needs. The target halogenide reagents and bis-thiadiazole ligands were obtained either as single products or as mixtures easily separable by chromatography. Characterisation of the products was performed using 1D and 2D NMR spectra in solution, complemented by single crystal X-ray diffraction (XRD) for selected samples, to elucidate their structural properties.

Evaluating Puncture and Non-puncture for the Turbo Code Model based the on AWGN Channel with 16-QAM

In general, a 5G new radio network is defined by three primary utilization situations, namely upgraded versatile broadband, ultra-reliable, and low latency interchanges of information, as well as huge machine-type correspondences. These utilizations require improved throughput, inertness, dependability contrasted, and a 4G framework. As a coding plan for information, 5G Turbo codes attain high throughput, a variable code rate, and length, and crossover programmed rehash with good error-detecting capability. The execution boundaries of 5G advancements are normal and are tens and thousands of times better compared with 4G. In this paper, the evaluations of puncturing and non-puncturing for turbo code depending on the minimization of penetrating of efficient pieces are discussed. It also provides penetrating of equality pieces considering additive white Gaussian noise (AWGN) Channel with 16 quadrature amplitude modulation (QAM) to attain high spectral efficiency. In addition to this, the super encoder encodes the separated progression with a code speed of 1/3. The code block association progressively connects the yields from the super encoder. The sign mapper employed in this paper adjusts the progression using a 16-QAM balance. Also, the mapper takes effective pieces, one uniformity bit from the upper encoder, and the deinterleaved equity bit from the base encoder. The Orthogonal frequency-division multiplexing (OFDM) mapper parts into more modest equivalent channels, named subcarriers and the information is sent on these equivalent channels at a reduced rate.

RNase E searches for cleavage sites in RNA by linear diffusion: direct evidence from single-molecule FRET.

The ability of obstacles in cellular transcripts to protect downstream but not upstream sites en masse from attack by RNase E has prompted the hypothesis that this mRNA-degrading endonuclease may scan 5'-monophosphorylated RNA linearly for cleavage sites, starting at the 5' end. However, despite its proposed regulatory importance, the migration of RNase E on RNA has never been directly observed. We have now used single-molecule FRET to monitor the dynamics of this homotetrameric enzyme on RNA. Our findings reveal that RNase E slides along unpaired regions of RNA without consuming a molecular source of energy such as ATP and that its forward progress can be impeded when it encounters a large structural discontinuity. This movement, which is bidirectional, occurs in discrete steps of variable length and requires an RNA ligand much longer than needed to occupy a single RNase E subunit. These results indicate that RNase E scans for cleavage sites by one-dimensional diffusion and suggest a possible molecular mechanism.

Fabrication of Size‐Coded Amphiphilic Particles with a Configurable 3D‐Printed Microfluidic Device for the Formation of Particle‐Templated Droplets

AbstractCompartmentalizing an aqueous media into numerous nanoliter‐scale droplets has substantially improved the performance of amplification assays. Particle‐templated droplets or dropicles offer a user‐friendly workflow for creating uniform volume compartments upon simple mixing of reagents and particles using common laboratory apparatus. Amphiphilic shape‐coded particles are demonstrated to spontaneously hold aqueous droplets within hydrophilic cavities for multiplexed diagnostic assays. Here, a configurable 3D‐printed microfluidic device is proposed for the tunable fabrication of amphiphilic size‐coded particles. The device is configured with multiple outlet tubings of different diameters and photomasks of variable slit lengths to fabricate a wide range of size‐coded particles. a range of unique particle codes are fabricated using a single reconfigurable device. The cross‐sectional profile of the particles is further engineered by tuning the flow rate ratios of precursor streams to vary the inner and outer diameters of the particles and the thicknesses of the inner hydrophilic and outer hydrophobic layers. A range of cavity diameters and particle lengths enabled dropicle volumes of ≈1 nL to ≈30 nL. The fabricated particles are characterized by their ability to hold uniform droplet volumes and to orient themselves facing upward or sideways in a well plate based on their aspect ratios.

Do Balloon Catheters have a Different Radial Force Along Their Longitudinal Axis?

PurposeThis experimental study was designed to compare radial forces between the central portion and both ends of balloon catheters when dilating stenosis.Materials and MethodsThree balloon catheters of 6 and 8 mm in diameter and of variable length were tested: Mustang, Conquest, and Genoss PTA. Cylindrical modules to position balloon catheters and install the measuring tip during radial force measurements were made using a 3D printer. The measuring tip created 20% stenosis at the inner lumen. Both ends and center of the balloon catheter were located at the measuring tip. The radial force was measured after inflating the balloon catheter to the rated burst pressure.ResultsFor the different diameters and lengths of balloon catheters and cylinder sizes, the median inccenter, the radial rease in radial force at the distal end compared to the center was 16.5% (range: 9.8–35.2%) for Mustang, 12.4% (range: 10.3–25.5%) for Genoss, and 7.4% (range: −0.3–13.1%) for Conquest balloon catheters. Similarly, compared to that at the force at the proximal end was 10.8% greater (range: −2.9–18.3%) for Mustang, 9.9% greater (range: 3.9–22.3%) for Genoss, and 7.3% greater (range: −1.3–12.4%) for Conquest catheters.ConclusionThe radial force is greater at both ends of the balloon than at the central portion, especially at the distal end. Dilation using the distal end of the balloon catheter is a practical method that can be applied in clinical practice without additional devices when encountering resistant stenosis, especially with semi-compliant balloons.Graphical

160 Gbps MMF/FSO system based on OAM beams and PV code under rainy weather

In this paper, a high-speed transmission system that combines a multimode fiber (MMF) with Free Space Optics (FSO) is proposed to accommodate the rapid growth of traffic. As Orbital Angular Beams Multiplexing (OAM) plays a crucial role in 6G networks by enhancing transmission capacity, four OAM beams (LG0,0\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${LG}_{\\mathrm{0,0}}$$\\end{document}, LG0,20\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${LG}_{\\mathrm{0,20}}$$\\end{document}, LG0,40\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${LG}_{\\mathrm{0,40}}$$\\end{document}, and LG0,40\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${LG}_{\\mathrm{0,40}}$$\\end{document}) are utilized in this study. Additionally, the Optical Code Division Multiple Access (OCDMA) technique is employed, known for its high level of confidentiality and the ability to allow multiple channels to transmit data simultaneously. To ensure ubiquitous data transmission, two channels: MMF and FSO are used. Accordingly, in our proposed MMF/FSO system, we employ four OAM beams, with each carrying four OCDMA channels assigned distinct Permutation Vector (PV) codes. With a data transmission rate of 10 Gbps on each OCDMA channel, the overall capacity of the system amounts to 160 Gbps (10 Gbps × 4 OCDMA channels × 4 OAM beams). Moreover, the performance is investigated and evaluated using two scenarios. The first assumes an FSO range of 5 km and Clear Air (CA) weather conditions, with a variable MMF length. In contrast, the second scenario maintains a fixed MMF length of 1 km while altering the FSO span. Weather conditions like CA and rain conditions; Light Rain (LR), Medium Rain (MR), and Heavy Rain (HR) are considered while evaluating the system performance in the second case. The bit error rate (BER), travelling distances in the channel either MMF or FSO, and eye diagrams are among the metrics used for evaluation. They all provide information about the received signal performance. Finally, the obtained results for the proposed MMF/FSO system, utilizing OAM beams and PV codes, indicate that in the first scenario, the system can support a total capacity of 160 Gbps across all channels. The performance is noteworthy, with a BER below 10-5\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${10}^{-5}$$\\end{document} and a wide eye opening, enabling successful transmission over 6.2 km (1.2 km MMF length + fixed 5 km FSO span). For the second scenario, the achievable distance is decreased due to the attenuation caused by rain. It becomes 2.62 km (1 km MMF + 1.62 km FSO span), 5.2 km (1 km MMF + 1.2 km FSO span), and 1.77 km (1 km MMF + 0.77 km FSO span), under LR, MR, and HR, respectively.

Dynamics of a heavy pendulum of variable length with a movable suspension point

Dynamics of a heavy pendulum of variable length with a movable suspension point

Curves that allow the motion of a regular polygon

AbstractThis paper characterizes curves where a regular polygon of either a variable side length or a constant side length is allowed to rotate during k full revolutions while having its vertices on the curve during the motion. A constructive method to generate these curves is given based on the curve described by the polygon centers (centroids) during the motion and some examples are shown. Moreover, if the regular polygon divides the curve perimeter into parts of equal length, it is proved that the curve is either a rotational symmetric curve in the case of a variable side length or a circle otherwise. Finally, in the case of a regular polygon of constant side length rotating along a curve, a simple relation between the algebraic areas of such a curve and the curve of polygon centers is revisited.

Several Approaches for the Prediction of the Operating Modes of a Wind Turbine

Growing concern about climate change has intensified efforts to use renewable energy, with wind energy highlighted as a growing source. It is known that wind turbines are characterized by distinct operating modes that reflect production efficiency. In this work, we focus on the forecasting problem for univariate discrete-valued time series of operating modes. We define three prediction strategies to overcome the difficulties associated with missing data. These strategies are evaluated through experiments using five forecasting methods across two real-life datasets. Two of the forecasting methods have been introduced in the statistical literature as extensions of the well-known context algorithm: variable length Markov chains and Bayesian context tree. Additionally, we consider a Bayesian method based on conditional tensor factorization and two different smoothers from the classical tools for time series forecasting. After evaluating each pair prediction strategy/forecasting method in terms of prediction accuracy versus computational complexity, we provide guidance on the methods that are suitable for forecasting the time series of operating modes. The prediction results that we report demonstrate that high accuracy can be achieved with reduced computational resources.

In-silico Evaluation of Mirror Repeats in Some Selected Genes of Candida albicans

All cellular processes in a living cell are controlled by its genetic material. DNA in majority of the domains acts as a regulatory molecule by controlling various vital functions. The genetic makeup of DNA or RNA (in viruses) is unique in all the domains. Their uniqueness is determined by the presence of various types of repetitive patterns of bases. These includes inverted repeats, tandem repeats, VNTR’s, palindromes etc. Among many repetitive pattern types, Mirror repeats (MR) found to be dispersed throughout the genes or genomes. These sequences are associated with various functional features like their involvement in H-DNA formation, in replication &amp; transcription, nervous system related diseases development etc. The major focus of this investigation is to identify MR sequences from some selected genes of Candida albicans using a bioinformatics based pipeline. The approach refers to as FPCB which utilized some manual steps to extract out MR sequence from any targeted gene or genome. The current study find out that the identified Mirror repeats found to be dispersed throughout the selected genes along with variable length. Among them the maximum &amp; minimum MR sequences were reported in the gene FAS2 (108) &amp; HIS1 (15) respectively. The present study will be helpful to provide a new insight for molecular as well as computational based studies of Candida albicans as well as other related fungal groups.

Study on the variable length simple pendulum oscillation based on the relative mode transfer method.

In this study, we employed the principle of Relative Mode Transfer Method (RMTM) to establish a model for a single pendulum subjected to sudden changes in its length. An experimental platform for image processing was constructed to accurately track the position of a moving ball, enabling experimental verification of the pendulum's motion under specific operating conditions. The experimental data demonstrated excellent agreement with simulated numerical results, validating the effectiveness of the proposed methodology. Furthermore, we performed simulations of a double obstacle pendulum system, investigating the effects of different parameters, including obstacle pin positions, quantities, and initial release angles, on the pendulum's motion through numerical simulations. This research provides novel insights into addressing the challenges associated with abrupt and continuous changes in pendulum length.