Segmentation Variables Research Articles

Segmentation of potential film tourists by film nostalgia and preferred film tourism program

ABSTRACTThe current study sought to identify the role of nostalgia in film tourism, specifically in relation to the development of preferred film tourism products and activities for potential Korean film tourists visiting the shooting locations of Hong Kong films made from the 1970s to the late 1990s. The findings suggest that the concept of nostalgia in the context of film tourism is multidimensional and consists of five identified domains. Of these domains, “reminiscence of mimicking”, “memory of film backdrops”, and “memory of Hong Kong history and culture” were highly significant in explaining familiarity with film destinations and the intention to engage in nostalgia film tourism. Differences in characteristics of respondents’ profiles were categorized into clusters generated using extracted domains. Among the four extracted clusters, the high film nostalgia group (Cluster 2) and the low film nostalgia group (Cluster 3) showed completely different characteristics. These differences included preferred activities or tour programs and socio-demographic profiles. As a result, this study found film nostalgia to be an important segmentation variable. The results of this study can be prudently used by film destination marketers to develop new products or programs and to satisfy their on-site experiences.

Abstract WP244: Review Criteria For Transfer Elderly Patient From Community Hospital To Comprehensive Stroke Center

Background: Recent guidelines recommend thrombectomy to treat stroke due to large vessel occlusions within first six hours after onset stroke time. There is no age limit, however elderly patients (>=80 years old) transferred from community hospital (CH) to comprehensive stroke center (CSC) possibly present different radiological findings in comparison with younger patients, that finally exclude them of endovascular treatment and could avoid unnecessary transfers to CSC. Method: We reviewed consecutive patients transferred from five CH to our CSC from January 2012 to July 2016. We analyzed radiological findings in ASPECTS (Alberta Stroke Program Early CT Scale) and segment of occluded artery in NonContrastCT (NCCT) and CT-angiography, both realized in CH (pre-transfer) and CSC. We evaluated the changes in NCCT between both centers related to onset stroke time, time of transfer, vascular occlusion segment and other epidemiological variables. Results: A total of 3825 patients were evaluated in our CSC as stroke code (24% elderly and increasing annually); 21% were transferred from CH to CSC with large vessel occlusion diagnosis and NIHSS>8. Subgroup of elderly patients presented worse score in ASPECTS-NCCT evaluated at CSC previous to endovascular treatment than younger patients (mean 6.8 vs 8.4;p: 0.001). An ASPECT score <8 in NCCT performed at CH pre-transfer was significant associated to important ASPECT score decay of respective NCCT performed at CSC for endovascular treatment assessment. This age-association was not demonstrated in younger patients. The occluded segments TICA and M1 were associated to lower ASPECTS. As a result less than 30% elderly patients transferred to CSC for thrombectomy were treated due to low ASPECT score in NCCT performed in CSC; confirmed by CT-perfusion. In ROC analysis we found a cut-off of 83 years for patients with good ASPECTS (dichotomized as ASPECTS>6) in NCCT at CSC (AUC: 0.69; S: 61%, E: 85%). Conclusions: Patients over 83 years old and low score in ASPECT (score<8) evaluated in CH presents low benefit when they are transferred to CSC in our area. Criteria to transfer elderly patient from CH to CSC for possible endovascular treatment must be reviewed according new guidelines to get efficient our Emergency Departments.

Identifying the critical factors that influence intraocular pressure using an automated regression tree

Background: Assessment of intraocular pressure (IOP) is an important test in glaucoma. In addition, anterior segment variables may be useful in screening for glaucoma risk. Studies have investigated the associations between IOP and anterior segment variables using traditional statistical methods. The classification and regression tree (CART) method provides another dimension to detect important variables in a relationship automatically.Aim: To identify the critical factors that influence IOP using a regression tree.Methods: A quantitative cross-sectional research design was used. Anterior segment variables were measured in 700 participants using the iVue100 optical coherence tomographer, Oculus Keratograph and Nidek US-500 ultrasonographer. A Goldmann applanation tonometer was used to measure IOP. Data from only the right eyes were analysed because of high levels of interocular symmetry. A regression tree model was generated with the CART method and Pearson’s correlation coefficients were used to assess the relationships between the ocular variables.Results: The mean IOP for the entire sample was 14.63 mmHg ± 2.40 mmHg. The CART method selected three anterior segment variables in the regression tree model. Central corneal thickness was the most important variable with a cut-off value of 527 µm. The other important variables included average paracentral corneal thickness and axial anterior chamber depth. Corneal thickness measurements increased towards the periphery and were significantly correlated with IOP (r ≥ 0.50, p ≤ 0.001).Conclusion: The CART method identified the anterior segment variables that influenced IOP. Understanding the relationship between IOP and anterior segment variables may help to clinically identify patients with ocular risk factors associated with elevated IOPs.

A comparison of gluteus medius, gluteus minimus and tensor facia latae muscle activation during gait in post-menopausal women with and without greater trochanteric pain syndrome

The effect of greater trochanteric pain syndrome (GTPS) on gluteus medius (GMed) and minimus (GMin) activation in post-menopausal women is unknown. The aim of this study was to compare segmental muscle activation and variability of the GMed, GMin and tensor fascia latae (TFL) during gait in post-menopausal women with and without GTPS.Intramuscular electrodes were inserted into segments of GMin (x2) and GMed (x3) and a surface electrode placed on TFL. Ten control participants and 8 with GTPS completed six walking trials. Peak amplitude, average amplitude and time to peak from each phase of the gait cycle (0–30%, 30%- toe off (TO), total stance and swing) were compared between groups using independent t-tests and effect-size (ES) calculations. Variability of muscle activation was calculated using the mean coefficient of variation (CV). Reversal of anterior GMin electromyographic burst pattern and greater average muscle activity was found in the GTPS group compared to controls: 0-TO for anterior GMin (p<0.05), anterior and middle GMed (p<0.01); 0–30% for posterior GMin (p<0.01) and GMed (p<0.05). No significant differences were identified in TFL. Overall, this study found increased segmental gluteal muscle activation, decreased hip abduction strength, and reduced variability in muscle activation in post-menopausal women with GTPS, compared with controls.

Pengaruh segmentasi pasar terhadap kepuasan pelanggan (nasabah) pada Bank BPR Jatim Cabang Ponorogo

&lt;p&gt;&lt;em&gt;This study aims to determine the effect of market segmentation of customer satisfaction (Customer) in bank BPR Jatim Branch of Ponorogo. Market Segmentation effect on customer satisfaction as evidenced from t value on the market cementation variable (X) is from a significant level of 0.000 t less than 0.05 then the research hypothesis H0 and menerimaHa resisting. And it can be seen the value rhitung is 0.748 while rtabel 0,138. This&lt;/em&gt;&lt;/p&gt;&lt;p&gt; &lt;em&gt;constant value of 10.264 means if the variable value market segmentation fixed or constant, the amount of customer satisfaction (customers) amounted to 10.264. This means that in the absence of market segmentation variables pegaruh then customer satisfaction still has a value of 10.264.&lt;/em&gt;&lt;/p&gt;

Analysis of the impact of length of stay on the quality of service experience, satisfaction and loyalty

Although length of stay is a relevant variable in destination management, little research has been produced connecting it with tourists’ post-consumption behaviour. This research compares the post-consumption behaviour of same-day visitors with overnight tourists in a sample of 398 domestic vacationers at two Mediterranean heritage-and-beach destinations. Although economic research on length of stay posits that there are destination benefits in longer stays, same-day visitors score higher in most of the post-consumption variables under study. Significant differences arise in hedonic aspects of the tourist experience and destination loyalty. Thus, we propose that length of stay can be used as a segmentation variable. Furthermore, destination management organisations need to consider length of stay when designing tourism policies. The tourist product and communication strategies might be adapted to different vacation durations.

Bioelectrical Impedance Analysis of Body Composition in Karate Athletes Regarding The Preparatory Period // Analiza telesnog sastava karatista bioelektričnom impedansom pre i posle pripremnog perioda

The aim of the paper was to asses changes in body composition using bioelectrical impedance analysis (BIA) methodology in members of national karate team after teen week preparatory training period. The investigation was carried out on 11 male karate contestants, aged 18 to 28 years mean age (21.82± 3.58). The body composition was analyzed with In Body 720. The BIA outcomes were divided in 3 group of variables: body fluid and body composition variables, obesity diagnose variables and segmental analysis variables. All BIA variables were insignificantly higher at second measuring (p&gt;005). Only Body mass index (BMI=24.1 vs 24.55); mineral (4.69 kg vs 4.77 kg) and osseous (3.85 kg vs 3.92 kg), were significantly higher (p≤0.05) after preparatory period. Body fat mass (BFM=10.34 kg vs 10.75 kg, p=0.329) and body fat percent (BF%= 12.73 vs13.22%) insignificantly increased after preparatory period. The skeletal mass has changed from 40.03kg to 40.55kg (p=0.276). Body composition analysis, changes in weight, BMI and body fluids are essential for weight categories dependent sports such as karate. Positive changes in body components and in body fluids suggest that the training process during the preparatory period did not show negative effects on body components and the hydration of the karate athletes.

Segmenting emerging markets based on consumer responses towards social network advertising

A wealth of new digital technologies have enhanced the reach of marketers and enabled them to interact with consumers in a big way in emerging markets like India. The onset of the digital era has resulted in social network advertising (SNA) becoming an integral part of the communication mix of firms. The current study attempts to offer a more subtle understanding of consumer engagement with social media by closely examining consumer responses to social network advertising and the consequences thereof. It uses psychographic and other lifestyle variables as segmentation variables. Hence it will particularly be of interest to marketers and researchers on the lookout for upcoming marketing opportunities. Also, the study investigates how advertising on social networks impacts preferences of consumers; and their acceptance and avoidance of this advertising, in terms of their belief in ads appearing on social networking sites.

Addressing the Barriers to Bioimpedance Spectroscopy Use in Major Burns: Alternate Electrode Placement.

Bioimpedance spectroscopy (BIS) is a method used to assess body composition and fluid distribution. As a technology for measurement of fluid shifts during acute burn resuscitation, there are potential barriers to its use due to the location of wounds. This study aimed to determine whether alternate electrode positions were a suitable alternative compared to standardized (manufacturer) positions in moderate to large size burns for the measurement of BIS resistance and fluid changes. BIS measurements were collected in standard and alternate electrode placements and in an open wound and Acticoat™ dressing condition. A percentage difference greater than 5% between each standard and alternate placement BIS measurements was deemed clinically significant. Chi-square tests determined there were no significant differences (P = .097-.96) between the standard and alternate electrode placements for whole body and limb segment BIS in both dressing conditions. Only whole body BIS resistance variables and extracellular fluid volumes were interchangeable in both dressing conditions and upper limb segmental measures were interchangeable in an open wound only. The differences between measurements of other BIS variables across the conditions were not acceptable or deemed not clinically acceptable without adjustment. The results showed that for moderate to large burn injuries clinicians can use whole body and upper limb segmental BIS variables to monitor changes in fluid shifts with alternate electrode placements where wounds preclude standardized placement within specified dressing conditions.

An exploratory study of the influence of load and practice on segmental and articulatory variability in children with speech sound disorders

ABSTRACTThis exploratory treatment study used phonetic transcription and speech kinematics to examine changes in segmental and articulatory variability. Nine children, ages 4 to 8 years old, served as participants, including two with childhood apraxia of speech (CAS), five with speech sound disorder (SSD) and two who were typically developing. Children practised producing agent + action phrases in an imitation task (low linguistic load) and a retrieval task (high linguistic load) over five sessions. In the imitation task in session one, both participants with CAS showed high degrees of segmental and articulatory variability. After five sessions, imitation practice resulted in increased articulatory variability for five participants. Retrieval practice resulted in decreased articulatory variability in three participants with SSD. These results suggest that short-term speech production practice in rote imitation disrupts articulatory control in children with and without CAS. In contrast, tasks that require linguistic processing may scaffold learning for children with SSD but not CAS.

Cardiovascular magnetic resonance myocardial feature tracking using a non-rigid, elastic image registration algorithm: assessment of variability in a real-life clinical setting

BackgroundCardiovascular magnetic resonance myocardial feature tracking (CMR-FT) is a promising technique for quantification of myocardial strain from steady-state free precession (SSFP) cine images. We sought to determine the variability of CMR-FT using a non-rigid elastic registration algorithm recently available in a commercial software package (Segment, Medviso) in a real-life clinical setting.MethodsFirstly, we studied the variability in a healthy volunteer who underwent 10 CMR studies over five consecutive days. Secondly, 10 patients were selected from our CMR database yielding normal findings (normal group). Finally, we prospectively studied 10 patients with known or suspected myocardial pathology referred for further investigation to CMR (patient group). In the patient group a second study was performed respecting an interval of 30 min between studies. All studies were manually segmented at the end-diastolic phase by three observers. In all subjects left ventricular (LV) circumferential and radial strain were calculated in the short-axis direction (EccSAX and ErrSAX, respectively) and longitudinal strain in the long-axis direction (EllLAX). The level of CMR experience of the observers was 2 weeks, 6 months and >20 years.ResultsMean contouring time was 7 ± 1 min, mean FT calculation time 13 ± 2 min. Intra- and inter-observer variability was good to excellent with an coefficient of reproducibility (CR) ranging 1.6% to 11.5%, and 1.7% to 16.0%, respectively and an intraclass correlation coefficient (ICC) ranging 0.89 to 1.00 and 0.74 to 0.99, respectively. Variability considerably increased in the test-retest setting with a CR ranging 4.2% to 29.1% and an ICC ranging 0.66 to 0.95 in the patient group. Variability was not influenced by level of expertise of the observers. Neither did the presence of myocardial pathology at CMR negatively impact variability. However, compared to global myocardial strain, segmental myocardial strain variability increased with a factor 2–3, in particular for the basal and apical short-axis slices.ConclusionsCMR-FT using non-rigid, elastic registration is a reproducible approach for strain analysis in patients routinely scheduled for CMR, and is not influenced by the level of training. However, further improvement is needed to reliably depict small variations in segmental myocardial strain.

Intra-rater variability in low-grade glioma segmentation.

Assessment of size and growth are key radiological factors in low-grade gliomas (LGGs), both for prognostication and treatment evaluation, but the reliability of LGG-segmentation is scarcely studied. With a diffuse and invasive growth pattern, usually without contrast enhancement, these tumors can be difficult to delineate. The aim of this study was to investigate the intra-observer variability in LGG-segmentation for a radiologist without prior segmentation experience. Pre-operative 3D FLAIR images of 23 LGGs were segmented three times in the software 3D Slicer. Tumor volumes were calculated, together with the absolute and relative difference between the segmentations. To quantify the intra-rater variability, we used the Jaccard coefficient comparing both two (J2) and three (J3) segmentations as well as the Hausdorff Distance (HD). The variability measured with J2 improved significantly between the two last segmentations compared to the two first, going from 0.87 to 0.90 (p = 0.04). Between the last two segmentations, larger tumors showed a tendency towards smaller relative volume difference (p = 0.07), while tumors with well-defined borders had significantly less variability measured with both J2 (p = 0.04) and HD (p < 0.01). We found no significant relationship between variability and histological sub-types or Apparent Diffusion Coefficients (ADC). We found that the intra-rater variability can be considerable in serial LGG-segmentation, but the variability seems to decrease with experience and higher grade of border conspicuity. Our findings highlight that some criteria defining tumor borders and progression in 3D volumetric segmentation is needed, if moving from 2D to 3D assessment of size and growth of LGGs.

Postediting prostate magnetic resonance imaging segmentation consistency and operator time using manual and computer-assisted segmentation: multiobserver study.

Prostate segmentation on T2w MRI is important for several diagnostic and therapeutic procedures for prostate cancer. Manual segmentation is time-consuming, labor-intensive, and subject to high interobserver variability. This study investigated the suitability of computer-assisted segmentation algorithms for clinical translation, based on measurements of interoperator variability and measurements of the editing time required to yield clinically acceptable segmentations. A multioperator pilot study was performed under three pre- and postediting conditions: manual, semiautomatic, and automatic segmentation. We recorded the required editing time for each segmentation and measured the editing magnitude based on five different spatial metrics. We recorded average editing times of 213, 328, and 393s for manual, semiautomatic, and automatic segmentation respectively, while an average fully manual segmentation time of 564s was recorded. The reduced measured postediting interoperator variability of semiautomatic and automatic segmentations compared to the manual approach indicates the potential of computer-assisted segmentation for generating a clinically acceptable segmentation faster with higher consistency. The lack of strong correlation between editing time and the values of typically used error metrics ([Formula: see text]) implies that the necessary postsegmentation editing time needs to be measured directly in order to evaluate an algorithm's suitability for clinical translation.

Outdoor recreation participants’ motivations, experiences and vacation activity preferences

This study investigates the behavioural relationship between outdoor recreation and vacation tourism in order to facilitate the marketing of nature activities as tourism products. In determining the outdoor recreation behaviour, motivations were explored on the basis of the recreation experience preference theory, and experiences were explored on the basis of the experience economy theory. In order to determine the vacation behaviour, which is an important market segmentation variable, vacation activity preferences were investigated. The implementation was accomplished within the scope of the outdoor recreationists in Eskişehir, Turkey. The data were collected through face-to-face and web surveys and analysed with the support of structural equation modelling. As the result of the analyses suggests, significant relationships were found among outdoor recreation motivations, nature experiences and vacation activity preference structures.

The Moderating Effects of User and Program Segmentation Variables from the Relationship between TV Program Ratings and Digital VoD Clip Views : An Exploration of Cues for the Neo-Double Jeopardy Phenomenon in Multimedia Environment

이 연구는 다중 매체 환경 내 TV 프로그램 시청률과 디지털 VoD 클립 조회 수 간 관계에서 특유의 이중 위험 현상이 발생될 수 있는가란 물음에서 시작됐다. 실증적 접근의 탐색 연구인만큼, 이용자의 주요 인구학적 속성 (성 · 연령대) 및 프로그램 속성 (채널 · 장르) 변인의 조절 역할을 알아보고자 했다. 이를 위하여, 지상파 3사·종편 4사·케이블 3사 프로그램에 대해 닐슨코리아가 제공하는 TV 시청률 데이터, SMR이 판매 대행하는 디지털 VoD 클립 조회 수를 분석 자료 (프로그램 N ＝ 530)로 삼았다. 연구 결과, TV 시청률이 디지털 VoD 클립 조회 수에 미치는 영향력은 유의한 것으로 검증됐다. 단 그 영향력은 이용자 집단이 남성일 때보다 여성일 때 더 크게 나타났다. 또 10대 이하, 40대 이상인 경우보다 20∼30대일 때 더 컸다. 프로그램 채널 면에서는 종합편성, 케이블, 지상파 채널 순으로 TV 시청률이 중요 역할을 했다. 끝으로 드라마나 영화·오락 프로그램 장르의 경우와 달리, 정보 프로그램 장르의 TV 시청률은 디지털 VoD 클립 조회 수에 대해 유의한 영향력을 행사하지 않았다. 신(新) 이중 위험 현상 발생 가능성은 관계주의 관점의 개인적 집단화 측면에서 이용자 인구학적, 프로그램 속성에 따라 차이가 날 수 있음을 암시하는 결과다. 이를 바탕으로 광고·방송 이론 및 실무 차원의 함의를 논의했다.

Use of Ethylmethylhydroxypyridine in Patients Presenting with Myocardial Infarction: Antiischemic, Antidepressant, Anxiolytic Effects

The objective: to evaluate the efficiency of ethylmethylhydroxypyridine (Mexiprim, STADA Arzneimittel AG, Germany) in patients presenting with myocardial infarction at hospital and outpatient stage.&#x0D; Patients and methods. The study included 59 patients with coronary artery disease, acute coronary syndrome with ST1segment elevation in the first day of admission to the ICU, AH, 3-stage, 2 degrees, HF. To all patients basic therapy according to current ESH/ESC guidelines was prescribed. To 39 patients additionally intravenous infusion of 200 mg of mexiprim o.d. for 10 days, followed by 125 mg per os three times a day for next 60 days was administered. Another 20 patients presented control group and received only basic therapy. The study design included: 24-hour Holter monitoring to estimate the dynamics of changes in the ST segment, cardiac arrhythmias and heart rate variability, evaluation by the scale of Beck, Hamilton scale for the assessment of anxiety (HARS) and depression (HDRS), the common blood and urine tests, biochemical blood analysis, evaluation of therapeutic tolerability conducted before treatment and 60 days after treatment. Surveys on a scale SAN, assessment of cognitive impairment on the MMSE scale were performed on the 60th day of treatment. Efficiency criteria were: a 50% reduction of cardiac arrhythmias, a decrease in ischemia, a decrease by 50% or more from baseline average score by HARS, HDRS scales, dynamics of the mental state questionnaire and less than 9 points on a scale of depression, reducing in SAN scale score.&#x0D; Results. In pаtients of mexiprim group significant reduction of depression scores by 62% were observed. According to the dynamics of the mental state questionnaire patients of mexiprim group reported feeling better, that is, reduction of score by 45% . According to the Hamilton scale for the assessment of anxiety (HARS), in particular mental anxiety – decrease in the total score of 65%, somatic anxiety – by 35.5%, and a total of 50% were revealed. In the group of patients receiving additionally intravenous Mexiprim for 10 days significantly reduced the number of single and group PACs, as well as single and multiple PVCs, not only in comparison with these parameters before the treatment, but also in comparison with the control group. In patients treated with Mexiprim no evidence of residual ischaemia were noted, but in the control group statistically significant segment depression ST remained. Heart rate variability was not significantly changed in the control group, but increased in patients who received Mexiprim.&#x0D; Conclusion. Use of Mexiprim in patients with myocardial infarction reduces ST segment depression, amount of ventricular and supraventricular arrhythmias, improved heart rate variability, and the state of anxiety and depression.

Sequential pattern formation governed by signaling gradients

Rhythmic and sequential segmentation of the embryonic body plan is a vital developmental patterning process in all vertebrate species. However, a theoretical framework capturing the emergence of dynamic patterns of gene expression from the interplay of cell oscillations with tissue elongation and shortening and with signaling gradients, is still missing. Here we show that a set of coupled genetic oscillators in an elongating tissue that is regulated by diffusing and advected signaling molecules can account for segmentation as a self-organized patterning process. This system can form a finite number of segments and the dynamics of segmentation and the total number of segments formed depend strongly on kinetic parameters describing tissue elongation and signaling molecules. The model accounts for existing experimental perturbations to signaling gradients, and makes testable predictions about novel perturbations. The variety of different patterns formed in our model can account for the variability of segmentation between different animal species.

English-learning infants’ perception of boundary tones

Like consonants and vowels, infants’ ability to discriminate lexical pitch becomes language-specific with age (Mattock & Burnham, 2006). We know less about when infants perceive pitch marking of prosodic units. We tested English-learning infants on Portuguese bisyllabic stimuli where statements and yes/no questions are segmentally identical but distinguished by a fall versus a rise on the final syllable. Using the visual habituation paradigm, we found that unlike Portuguese infants (Frota et al., 2014), only English 8-, but not 4-month-olds are able to distinguish final rise from fall in the presence of segmental variability. Besides having a rising pitch, the final syllable in Portuguese questions is also longer. We are currently testing English 8-month-olds with resynthesized Portuguese stimuli that maintain the pitch difference, but neutralize duration. Together, the results will help us reconcile the previously reported failure of English-learning infants to distinguish English statements from questio...

Relationship between Psychological and Socio-Cultural Adaptation as Perceived by International Students of United Arab Emirates

The research endeavors to shed light on the relationship between the psychological and socio-cultural adaptation of international students as perceived by students of UAE. Also, it aims to identify the segmentation of UAE bound international students. The research design is quantitative and utilized a descriptive survey methodology to examine the relationships and difference between the variables. It employs input, process, output model to respond to the research objectives and hypothesis poses at the onset of the study. The input involves the demographic profile of international students which highlighted the continental grouping, market segmentation, and other variables. The process involves the utilization of survey questionnaires, the collection of data, statistical tools, statistical treatment, Likert scale, interpretation, and analysis. The output is the proposed student development program. The study revealed significant findings on Psychological and Socio-Cultural Adaption, Demographic Profile and Market Segmentation which provided a new breadth of contribution to the body of knowledge. Based on the results, recommendations in conjunction with conclusions are introduced to pave the way for a proposed student development program. A sound and a workable developmental program for the international students will ensure a smooth and a successful integration of students in the United Arab Emirates.

Combining Tpi and CO1 Genetic Markers to Discriminate Invasive Helicoverpa armigera From Local Helicoverpa zea (Lepidoptera: Noctuidae) Populations in the Southeastern United States.

The recent establishment of the Old World pest Helicoverpa armigera (Hübner) into South America has had significant economic consequences and places the rest of the hemisphere at risk, emphasizing the need for improved methods of monitoring. A major complication is that a sibling species endemic to the New World, Helicoverpa zea (Boddie), is morphologically very similar, with the two species capable of producing fertile hybrids in the laboratory. The consequences of such hybridization in the field are uncertain, but could result in significant and unpredictable changes in the timing, range, and pesticide susceptibilities of Helicoverpa infestations. The objective here is to provide new genetic resources applicable to Helicoverpa populations in northern Florida and neighboring states (a region at risk for H. armigera) that can distinguish the two species and possible hybrids. The genetic variability in segments of the mitochondrial cytochrome oxidase 1 (CO1) and the Z-linked triosephosphate isomerase (Tpi) genes were determined for H. zea from the southeastern United States. These were compared to DNA sequences from H. armigera specimens from Morocco, Australia, and Europe. Phylogenetic network analysis showed a clear demarcation between the two species for all gene segments. These results extend earlier studies establishing CO1 as marker for discriminating the Helicoverpa species complex and introduce a new sex-linked genomic marker. The CO1 and Tpi markers in combination provide a more accurate and sensitive method than existing techniques for identifying hybridization between H. zea and H. armigera and could potentially be used to extrapolate the likely source of invasive H. armigera populations.