Viola canescens Wall. Ex., Roxb., also known as Himalayan white violet, belongs to the Violaceae family. It is widespread in Pakistan, India, Nepal, China, and Bhutan, with a specific presence in Himachal Pradesh, Uttarakhand, Uttar Pradesh, Arunachal Pradesh, Jammu and Kashmir, Meghalaya, Sikkim, and West Bengal in India. This study commenced with morphological analysis, followed by ultra-structural and genetic characterization using SSR markers. V. canescens populations were surveyed in the Northwestern Himalayan regions of Uttarakhand, Jammu and Kashmir, and Himachal Pradesh. Qualitative traits aligned with prior records, but variations in quantitative traits were noted. This morphological exploration serves as a foundation for future research endeavors and aids in the selection of plant populations for cultivation, offering valuable insights for ongoing and prospective studies.