Objective To investigate the value of ultrasound-guided liver biopsy for infantile hepatitis syndrome regarding diagnosis, treatment and prognosis. Methods Fifty children with infantile hepatitis syndrome hospita-lized in Guiyang Maternal and Child Hospital during Aug.2010 to May 2012 were involved in this study.Ultrasound-guided liver biopsies were performed to evaluate the inflammation grade and fibrosis stage.Immunohistochemical staining was used for pathogen diagnosis.The clinical outcomes were followed-up. Results Thirty-four cases(68%)were CMV infection, 6 cases(12%) were vanishing bile duct syndrome, 4 cases(8%) were chronic intrauterine infection, 4 cases(8%) were congenital anomaly of bilirubin metabolism, and 2 cases(4%) were obstructive cholangitis.All 50 cases showed mild inflammation at portal area(G1-G2 grade). All 50 cases exhibited liver fibrosis.Sixteen cases were S1 stage, 20 cases were S2 stage, 8 cases were S3 stage and 6 cases were S4 stage.Pathogen analysis: all 50 cases showed intrahepatic cholestasis: 38 cases were diffuse cholestasis, and 12 cases were moderate cholestasis.Treatment: all cases were treated using 2-week heteropathy; antivirus was used for CMV infected cases, thus 39 cases were finally cured, 9 cases were relieved, and 2 cases were ineffective. Conclusion Liver biopsy is valuable for diagnosis, treatment and prognosis infantile hepatitis syndrome. Key words: Infantile hepatitis syndrome; Ultrasound-guided liver biopsy; Liver pathology