This study was performed to investigate the prevalence of rotavirus, coronavirus, Cryptosporidium, E. coli F5 (K99), Cl. perfringens and Salmonella spp. as well as the clinical, hematological, biochemical alterations, and efficacy of lactoferrin supplementation in classical treatment in neonatal diarrheic calves in city of Van and Diyarbakir. 100 diarrheic calves were investigated in terms of the specified agents with immunochromatographic test kit and with conventional bacteriological methods. Rotavirus was detected in 51% of the calves, Cryptosporidium in 35%, Cl. perfringens in 23%, coronavirus 16%, E. coli F5 (K99) in 4%, Salmonella spp. in 2% of the calves were detected. Giardia spp. was not detected in any of the calves. While 65% of the calves had a single agent, 33% had multiple. Hematological, biochemical, and blood gas analyses were performed before and after treatment, and in vitro bactericidal and bacteriostatic effect of lactoferrin was investigated on E. coli O157, E. coli F5 and Salmonella enteriditis. When used for treatment, lactoferrin was found to be effective on the bacteria at 100 mg/ml and higher concentrations and it lowered mortality rate, as well as showing a positive effect on clinical parameters. It was concluded that lactoferrin can be used for protective and therapeutic purposes at 100 mg/ml, and higher doses and will be more efficient.
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