Van Breukelen Research Articles

Selectivity in Enrichment of cAMP-dependent Protein Kinase Regulatory Subunits Type I and Type II and Their Interactors Using Modified cAMP Affinity Resins

cAMP regulates cellular functions primarily by activating PKA. The involvement of PKAs in various signaling pathways occurring simultaneously in different cellular compartments necessitates stringent spatial and temporal regulation. This specificity is largely achieved by binding of PKA to protein scaffolds, whereby a distinct group of proteins called A kinase anchoring proteins (AKAPs) play a dominant role. AKAPs are a diverse family of proteins that all bind via a small PKA binding domain to the regulatory subunits of PKA. The binding affinities between PKA and several AKAPs can be different for different isoforms of the regulatory subunits of PKA. Here we employ a combination of affinity chromatography and mass spectrometry-based quantitative proteomics to investigate specificity in PKA-AKAP interactions. Three different immobilized cAMP analogs were used to enrich for PKA and its interacting proteins from several systems; HEK293 and RCC10 cells and rat lung and testis tissues. Stable isotope labeling was used to confidently identify and differentially quantify target proteins and their preferential binding affinity for the three different cAMP analogs. We were able to enrich all four isoforms of the regulatory subunits of PKA and concomitantly identify more than 10 AKAPs. A selective enrichment of the PKA RI isoforms could be achieved; which allowed us to unravel which AKAPs bind preferentially to the RI or RII regulatory domains of PKA. Of the twelve AKAPs detected, seven preferentially bound to RII, whereas the remaining five displayed at least dual specificity with a potential preference for RI. For some of these AKAPs our data provide the first insights into their specificity.

Comparative Phosphoproteomics of Zebrafish Fyn/Yes Morpholino Knockdown Embryos

The coordinated movement of cells is indispensable for normal vertebrate gastrulation. Several important players and signaling pathways have been identified in convergence and extension (CE) cell movements during gastrulation, including non-canonical Wnt signaling. Fyn and Yes, members of the Src family of kinases, are key regulators of CE movements as well. Here we investigated signaling pathways in early development by comparison of the phosphoproteome of wild type zebrafish embryos with Fyn/Yes knockdown embryos that display specific CE cell movement defects. For quantitation we used differential stable isotope labeling by reductive amination of peptides. Equal amounts of labeled peptides from wild type and Fyn/Yes knockdown embryos were mixed and analyzed by on-line reversed phase TiO(2)-reversed phase LC-MS/MS. Phosphorylated and non-phosphorylated peptides were quantified, and significant changes in protein expression and/or phosphorylation were detected. We identified 348 phosphoproteins of which 69 showed a decrease in phosphorylation in Fyn/Yes knockdown embryos and 72 showed an increase in phosphorylation. Among these phosphoproteins were known regulators of cell movements, including Adducin and PDLIM5. Our results indicate that quantitative phosphoproteomics combined with morpholino-mediated knockdowns can be used to identify novel signaling pathways that act in zebrafish development in vivo.

Differentiatie door coaches binnen amateursportteams: terecht of onterecht?

Differential treatment by coaches of amateur sports teams: right or wrong? Differential treatment by coaches of amateur sports teams: right or wrong? J.W.M. van Breukelen & W. Wesselius, Gedrag & Organisatie, volume 20, November 2007, nr. 4, pp. 427-444 A central assumption in the Leader-Member Exchange (LMX) theory is that leaders do not adopt a single style towards all members of their work unit, but treat the various team members differently. This may result in different kinds of working relationships between the leader and the various members ranging from formal to intense. The effects of these different LMX relationships are visible in important outcome variables such as job satisfaction and performance. Not only in working organizations but also in the context of sports differential treatment by the coach seems a relevant topic. In this article we describe the results of a field study among the players (N = 218) of 21 amateur sports teams. Firstly, we investigated on which aspects the coaches of these teams differentiated between the various team members and how these incidents of differential treatment were experienced by the players in terms of justice and fairness. In addition, we analyzed whether the frequency and evaluation of differential treatment was related to the players' enthusiasm and to team atmosphere and team performance. Social differentiation was appreciated less than task differentiation. Especially task differentiation proved to be important for team performance.

Rechtvaardigheidsonderzoek in Nederland

Justice research in the Netherlands Justice research in the Netherlands J. Syroit, H. Steensma & W. van Breukelen, Gedrag & Organisatie, volume 20, November 2007, nr. 4, pp. 321-326 This edition is dedicated to justice research in the Netherlands. The concepts justice, fairness and equity are introduced and the history of justice research and justice theory are described. After that, the nine contributions to this special issue are summarized. On the basis of a review of this and other recent literature we identify several trends in justice research. We conclude this introduction with an agenda for future research projects.

Spectroscopic follow-up of a cluster candidate at z= 1.45

We have obtained deep optical spectroscopic data of the highest-redshift cluster candidate (z ~ 1.4, CVB13) selected by Van Breukelen et al. (2006) in a photometric optical/infrared catalogue of the Subaru XMM-Newton Deep Field. The data, which comprise 104 targeted galaxies, were taken with the DEep Imaging Multi-Object Spectrograph (DEIMOS) on the Keck 2 telescope and yielded 31 secure redshifts in the range 1.25 10^14 M_sun and it may therefore be termed a cluster. There is an X-ray source at the cluster position which is marginally spatially resolved but whose X-ray spectrum is too hard to be thermal cluster emission. Its origin could be the summed X-ray emission from active galaxies in, and projected onto, the cluster. Serendipitously we have discovered a cluster at z = 1.28 with a mass of > 10^14 M_sun at the same position on the sky, comprising six spectroscopically confirmed cluster galaxies and at least one additional radio source. The selection of CVB13 for the cluster catalogue was evidently aided by the superposition of other, presumably lower-mass, structures, whereas the single cluster at z = 1.28 contained too few galaxies to be isolated by the same algorithm. Given the complicated nature of such structures, caution must be employed when measuring the mass function of putative high-redshift clusters with photometric techniques alone.

De structuur en het belang van werkwaarden: een vergelijking tussen drie analysetechnieken

Structure and importance of work values: a comparison between three data analysis techniques Structure and importance of work values: a comparison between three data analysis techniques J.W.M. van Breukelen, B. Zandbergen & F.M.T.A. Busing, Gedrag & Organisatie, volume 20, September 2007, nr. 3, pp. 272-302 Work values refer to the importance people attribute to the various aspects of a job, such as work content, salary, and colleagues. Generally, in studies on work values two questions are being answered: a) How important are the various aspects of a job for a certain sample of respondents as a whole or for certain subgroups, and b) What is the structure underlying the total set of work values under study? In this study three data analytic techniques are being investigated in analysing the answers of 417 respondents (299 men and 118 women). The three data analytic methods were principal component analysis (PCA), multidimensional scaling (MDS), and multidimensional unfolding (MDU). Of these three, PCA and MDS give information about the structure of the work values, whereas MDU shows the importance of the various work values in a visual plot. We describe and discuss the pro's and cons of these techniques using the data set mentioned above as an illustration. Our conclusion is that MDU is a welcome addition to both PCA and MDS in studies on work values. Firstly, MDU makes visible the importance of work values for the group respondents as a whole and for subgroups, if needed. At the same time, MDU gives indications about the structure of the work values under study.

Participatief leiderschap: mogelijkheden en beperkingen

Participative leadership: opportunities and limitations Participative leadership: opportunities and limitations Wim van Breukelen & René van der Vlist, Gedrag & Organisatie, Volume 18, June 2005, nr. 3, pp. 156-180. Leaders have to fulfil three major leadership functions: goal achievement, group maintenance and guaranteeing future effectiveness. In this article participative leadership is discussed. In this leadership style, leaders involve their subordinates in decision making related to setting goals, choosing appropriate strategies, solving problems and making changes in their team, department, or organization. It is not suggested here that participative leadership is a panacea, but it is considered to be the best approach in specific situations. This view is in line with the contingency approach of leadership. Participative leadership particularly offers great potential in uncertain situations, when tasks and problems at hand are unstructured, and when subordinates have additional knowledge, skills and competences. There are several barriers and stimulants that determine whether a participative style in these circumstances will actually be applied, or whether it will remain a theoretical option. The effectiveness of participative leadership is evaluated in view of the three generic leadership functions as mentioned, as well as a number of specific criteria for leadership effectiveness.

Are unshifted distributional models appropriate for response time?

Van Breukelen (this issue) provides an approach to using both response time (RT) and accuracy for (1) measuring latent abilities of participants even when they may trade speed for accuracy, and for (2) providing insight into the psychological processes underlying task performance. In this commentary, I focus on the second of these aims and assess how useful this approach is for exploring cognition. The approach is based on formulating statistical rather than substantive models on accuracy and RT. I focus here on the appropriateness of the RT component model. This RT model joins a family of recent statistical approaches to RT including those of Glickman, Gray, and Morales (in press); Peruggia, Van Zandt, and Chen (2002); Rouder, Lu, Speckman, Sun, and Jiang (in press); Schnipke and Scrams (1997); and Wenger and Gibson (2004). The advantage of these statistical rather than substantive models is robust estimation and sound inference when data are collected across disparate individuals and items. It is not hard to see how these statistical developments will lead to better substantive theory testing and development in cognitive psychology. Van Breukelen’s model for the ith participant’s RT to the j th item is given by:

Models for the statistics and mechanisms of response speed and accuracy

Van Breukelen offers a promising method for modeling both response speed and response accuracy. However, the underlying conception of both dependent measures is somewhat flawed, leading the author to conclude that the approach possesses limitations that, under revised assumptions, may not hold. The central misconception, and a set of related misconceptions, is addressed, and it is suggested that this approach holds a good deal of promise for application in the perceptual and cognitive sciences.

Reaction to “Comments on ‘The Effects of Leader-Member Exchange and Differential Treatment on Work Unit Commitment’: Distinguishing between Neutralizing and Moderating Effects”

Ye and Barbuto in 2004 made a number of interesting observations about use in 2002 by Van Breukelen, Konst, and Van der Vlist of the term 'neutralizer'. They advised caution when employing the term 'neutralizer', preferring instead the designation 'moderator'. Their comments underscore the importance of discussion on the nature of interaction effects in studies of leadership. Here we clarify our procedures in more detail and present the results of further analyses of our data.

Comments on "The effects of leader-member exchange and differential treatment on work unit commitment": distinguishing between neutralizing and moderating effects.

Van Breukelen, Konst, and van der Vlist in 2002 examined how members' perceptions of differential treatment from the leader affect the relationship between Leader-Member exchange and work unit commitment. This paper offers commentary, with specific focus on the judgment call for examining continuous effects across all levels of differential treatment. To justify the role of differential treatment as a neutralizer that weakens the influence of Leader-Member exchange on work unit commitment, the regression coefficients of work unit commitment on Leader-Member Exchange should be examined continuously with discriminable differential treatment. As a caution against using "neutralizer" based on the research results presented, "moderator" is suggested as the more appropriate descriptor of the role that differential treatment played. Also, the nature of the interaction between Leader-Member exchange quality and work unit commitment is explored, suggesting research should focus more on the main effects.

The first study of dementia within the cherokee nation of Oklahoma : R.W. Richter, M.F. Weiner, D. Persson, K.E. Taubman, D.B. Dean and R.N. Rosenberg. Alzheimer's Dis. Res. Ctr., University of Texas Southerwestern, Dallas, TX 75235, USA

Le niveau d’études est considéré comme un facteur de réserve. Ainsi, les adultes âgés de haut niveau d’études ont de meilleures performances mnésiques que ceux ayant un niveau d’études plus bas. L’objectif de cette étude était d’examiner le rôle de la métamémoire comme un médiateur potentiel des effets du niveau d’études sur la performance mnésique. Quatre-vingt-trois adultes âgés (60–80 ans) ont été divisés en deux sous-groupes selon leur niveau d’études (haut et bas niveau). La mémoire épisodique a été évaluée à l’aide d’une tâche de rappel indicé et la métamémoire par le Metamemory in Adulthood questionnaire (MIA). Les résultats montrent qu’un haut niveau d’études est associé à de meilleures capacités mnésiques et métamnésiques. Ils mettent également en évidence le rôle de la métamémoire comme médiateur de l’effet du niveau d’études sur la performance mnésique, puisque la fréquence d’utilisation de stratégies internes médiatise complètement l’effet du niveau d’études sur la mémoire chez des adultes âgés.Educational level is a factor of cognitive reserve and older adults with a higher level of formal education have a better memory performance than those having a lower educational level (Angel et al., 2010; Van Der Elst, Van Boxtel, Van Breukelen, & Jolles, 2005). Memory functioning can also be modulated by the beliefs and knowledge of a person about his/her own memory, that is, by his/her metamemory (Hultsch, Hertzog, & Dixon, 1987). The objective of this study was to examine the role of metamemory as a potential mediator of the effect of educational level on memory performance. Eighty-three older adults (60–80 years) participated in the experiment, they have been divided into two subgroups according to their educational level (high: 14.36 years and low level: 9.85 years). Episodic memory was evaluated with a cued recall task and metamemory by the Metamemory in Adulthood questionnaire (MIA). As shown by previous studies, results indicated that educational level had a significant effect on memory and metamemory, higher educational level was associated to better memory and metamemory capacities. At the MIA questionnaire, older adults with a high educational level affirmed using more internal and external strategies for learning, having higher motivation and perceiving less memory change with aging than older adults with a lower educational level. They also showed that the metamemory dimensions associated to the memory performance differed according to the educational level. For participants with a lower educational level, memory performance was correlated to the participants’ perception about their memory capacity and their knowledge about memory tasks, while for participants with a higher educational level, memory performance was correlated to the dimensions linked to memory control (strategies and motivation). Finally, the group effect was mediated by metamemory, specifically by the use of internal strategies. These results suggest that a prolonged educative experience would be associated to a better capacity to implement adapted strategies, which led individuals to maintain an optimal memory performance.

Metabolism of bears before, during, and after winter sleep.

Metabolism of bears before, during, and after winter sleep.

Heat Tolerance of a Death Valley Pupfish (Genus Cyprinodon)

Heat Tolerance of a Death Valley Pupfish (Genus Cyprinodon)

Rat hepatic polysome profiles and in vitro protein synthesis during hypoxia.

ARTICLESRat hepatic polysome profiles and in vitro protein synthesis during hypoxiaMI Surks, and M BerkowitzMI Surks, and M BerkowitzPublished Online:01 Jun 1971https://doi.org/10.1152/ajplegacy.1971.220.6.1606MoreSectionsPDF (891 KB)Download PDF ToolsExport citationAdd to favoritesGet permissionsTrack citations ShareShare onFacebookTwitterLinkedInWeChat Previous Back to Top Next Download PDF FiguresReferencesRelatedInformation Cited ByeIF6 rebinding dynamically couples ribosome maturation and translation23 March 2022 | Nature Communications, Vol. 13, No. 1Dissociation by Pelota, Hbs1 and ABCE1 of mammalian vacant 80S ribosomes and stalled elongation complexes29 March 2011 | The EMBO Journal, Vol. 30, No. 9Regulation of global protein translation and protein degradation in aerobic dormancy16 November 2008 | Molecular and Cellular Biochemistry, Vol. 323, No. 1-2Effects of environmental hypercapnia on animal physiology: A 13C NMR study of protein synthesis rates in the marine invertebrate Sipunculus nudusComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Vol. 144, No. 4High sensitivity to chronically elevated CO2 levels in a eurybathic marine sipunculidAquatic Toxicology, Vol. 70, No. 1Metabolic rate depression in animals: transcriptional and translational controlsBiological Reviews, Vol. 79, No. 1Characterization of a novel gene up-regulated during anoxia exposure in the marine snail, Littorina littoreaGene, Vol. 283, No. 1-2A Profile of the Metabolic Responses to Anoxia in Marine InvertebratesTranslational initiation is uncoupled from elongation at 18°C during mammalian hibernationFrank van Breukelen, and Sandra L. Martin1 November 2001 | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 281, No. 5Short-term anoxia does not impair protein turnover in isolated perfused turtle heartJournal of Comparative Physiology B, Vol. 164, No. 8Effect of reduced atmospheric pressure and fasting on transferrin synthesis in the ratLife Sciences, Vol. 29, No. 16Studies on hypoxiaExperimental and Molecular Pathology, Vol. 21, No. 2 More from this issue > Volume 220Issue 6June 1971Pages 1606-1609 Copyright & PermissionsCopyright © 1971 by American Physiological Societyhttps://doi.org/10.1152/ajplegacy.1971.220.6.1606PubMed5087807History Published online 1 June 1971 Published in print 1 June 1971 Metrics

Animal Behavior and Internal Drives

Animal Behavior and Internal Drives