Low-density Lipoprotein Values Research Articles

Relation of Serum Resist in with Body Mass Index and Lipid Profile in Hypertensive and Ischemic Heart Disease patients

Background: Resistin, a novel hormone, got its recognition as a regulator of lipid metabolism in obese rodents. Human researches proved its role mainly in inflammation and to lesser extent in obesity. Aim: To observe the relationship of serum resistin with body mass index (BMI) and lipid levels in hypertensives and ischemic heart disease patients as compared to normal subjects. Methodology: Eighty participants between the ages of thirty to fifty five years were distributed in four groups including normal subjects, first time diagnosed patients of hypertension, and first time diagnosed hypertensive cases of stable angina pectoris and myocardial infarction respectively. After history and general physical examination, fasting blood samples of the participants were tested for serum resistin by using the enzyme linked immunosorbent assay technique and lipid profile with commercially available enzymatic kits. Analysis of the data was performed by SPSS version17.0. Results: In patients of research groups, statistically raised levels (mean±SD) of BMI, and serum values of resistin, triglycerides, low-density lipoproteins (LDL) while decreased high density lipoproteins (HDL) levels were documented in comparison with healthy subjects. Conclusions: There are significantly higher values of body mass index, blood resistin, triglycerides and LDL while lower serum HDL levels in hypertensives and patients of ischemic heart disease as compared to normal participants. Keywords: Resistin, ischemic heart disease, lipid profile, body mass index

FEATURES OF ARTERIAL HYPERTENSION COMBINED WITH OBESITY ACCORDING TO NON-ALCOHOLIC FATTY LIVER DISEASE (STEATOSIS AND STEATOHEPATITIS)

This research was aimed to estimate the course of hypertension on the background of obesity and nonalcoholic fatty liver disease according to its stages (steatosis and steatohepatitis). Ninety patients were evaluated for anthropometric, general-clinical, laboratory, instrumental and immune-enzymatic research methods. With USG, the most characteristic features of fatty liver infiltration and the criteria for the distinction of steatosis from steatohepatitis were revealed, and on the basis of them 2 groups were formed. Thus, the progression of nonalcoholic fatty liver disease to the stage of steatohepatitis was accompanied by a more pronounced increase in blood pressure larger in comparison with steatosis of mass index of myocardium and anterior-posterior size of the left atrium, indicating the progression of structural myocardial disorders. Therefore, the presence of non-alcoholic steatohepatitis in patients with hypertension can be regarded as unfavorable prognostic criteria for the development of heart failure. In the presence of steatohepatitis, more marked changes in lipidograms were noticed for Low-Density Lipoprotein and Triglyceride values and a violation of the functional state of the liver with a cytolytic syndrome on the activity of AST and ALT and the total bilirubin, which may contribute to the progression of liver damage.

Effect of Different Level of Zeolite and Perlite on The Physiological Performance of Awassi Lambs

A total of 24 male Awassi lamb aged (4-6) months were used, to determine the effect of different level of zeolite and perlite on the physiological traits of Awassi sheep. Results show that te use of zeolite led to the presence of high significant differences at the level (P ≤ 0.05) of the studied values between the experimental groups and according to the weeks of the study, as the values varied between different weeks in the characteristics of the biochemical blood parameters and values, which are (both cholesterol values, the values of high-density lipoproteins, low-density lipoproteins, and fats). All treatments of zeolite and perylite and their mixture outperformed the control group, while the fourth treatment T4 outperformed the other treatments, followed by the third treatment T3 over the studied treatments T2 and T1.

Triple-combined hypolipidaemic therapy in familial hypercholesterolaemia: clinical cases

Background. The prevalence of heterozygous familial hypercholesterolaemia (HeFH) comprises 1 per 250 people. The risk of premature cardiovascular disease (CVD) is 20 times higher in HeFH patients among the general population. CVD develops in HeFH patients under 20 years of age, and they usually do not survive to 30 years. Therefore, the primary treatment track here is correction of dyslipidaemia to prevent atherosclerosis progression and CVD. Clinical Case Descriptions. The article describes the clinical cases of familial dyslipidaemia in 47-yo patient M. and his 75-yo mother P. The patient had a visit related to blood pressure (BP) surges up to 140/90 mm Hg. In history: acute myocardial infarction (AMI) in maternal grandfather at 50 years and own uncle at 32 years. The patient’s cardiovascular risk factors: male gender, dyslipidaemia (total cholesterol (TC) 15.8 mmol/L), overweight (body mass index 29.9 kg/m2), familial history of young CVD, sedentary lifestyle (employed as manager), psychological and socioeconomic factors (work-related stress pressure), resting heart rate 88 beats/min. The patient was immediately ordered a combined hypolipidaemic therapy including rosuvastatin 20 mg, ezetimibe 10 mg, telmisartan 40 mg once daily for blood pressure correction. In 1-month therapy, cholesterol dropped to 4.4 mmol/L, low-density lipoprotein (LDL) cholesterol – to 2.2, but triglycerides remained high at 3.9 mmol/L. Fenofi brate added to therapy at 145 mg 1 time. Another 1-month therapy allowed the overall reduction of TC to 3.7, LDL cholesterol to 1.9, triglycerides to 2.17 and high-density lipoproteins to 1.19 mmol/L. Past 3 months, a further drop was observed in triglycerides to 1.7 mmol/L. Hence, a triple hypolipidaemic therapy facilitated the target LDL and triglyceride values without involving expensive medications like PCSK9 blockers. The patient’s mother also achieved the target basic lipidogram owing to a triple lipid-lowering therapy.Conclusion. The case is of interest to exemplify a successful triple lipid-lowering therapy in patients with familial hypercholesterolaemia.

The monocyte/high-density lipoprotein ratio predicts coronary artery disease in heart failure

Abstract Background The monocyte/high density lipoprotein ratio (MHR) is a combined inflammatory marker implicated in atherosclerosis. MHR predicts cerebrovascular disease (CVD) and chronic kidney disease (CKD); it correlates with coronary artery disease (CAD) severity and has been shown to be a prognostic predictor in patients with myocardial infarction. The role of MHR in chronic heart failure (HF) is largely unknown. Purpose Assess the ability of MHR to predict CAD in patients with HF Methods We conducted a retrospective cohort study in ambulatory patients with HF with left ventricular systolic dysfunction (LVSD) that were followed in our HF clinic from January/2012 to May/2018. We selected all the patients that had performed a diagnostic/therapeutic coronary angiography. Patients with missing data concerning monocyte counts or high-density lipoprotein levels were excluded. The endpoint under analysis was the presence of CAD on coronary angiography. A ROC curve was used to study the ability of MHR to predict CAD. The association of MHR with CAD was assessed by a logistic-regression analysis. A multivariate model was built accounting for age, sex, comorbidities [hypertension, diabetes, peripheral artery disease (PAD), CVD, CKD and inflammatory/auto-immune disease], low-density lipoprotein value, haemoglobin, brain-type natriuretic peptide (BNP), severe LVSD and segmental contractility defects in the echocardiogram. MHR was analysed both as continuous and categorical variable; the cut-off of 16 was chosen based on the ROC curve. Results We studied 295 chronic HF patients with LSVD who underwent coronary angiography. Mean age was 67±12 years, 70.8% men and 55.9% with CAD. Patients with CAD were older (69 against 64 years), had significantly higher MHR (19±9 vs. 16±7), higher prevalence of hypertension, diabetes, PAD and CVD, and worse renal function. The area under the curve for the association between MHR and CAD was 0.68 (0.62–0.74), p<0.001. The best MHR cut-off for CAD prediction was 16 - sensitivity: 66.7%, specificity: 65.4%, positive predictive value: 71.0% and negative predictive value: 60.8%. This association was independent of potential confounders. Patients with MHR≥16 had a multivariate-adjusted OR of having CAD of 5.41 (95% CI: 2.40–12.20), p<0.001 when compared to those with lower MHR. When MHR was analysed as a continuous variable the association remained significant. Conclusions The MHR is an independent predictor of CAD in chronic HF. Patients with MHR ≥16 have a 71% probability of presenting CAD and a more than 5-fold higher risk compared with those with lower MHR. It is sometimes difficult to ascertain ischemic disease/aetiology in HF patients, particularly in the elderly, frail and/or with advanced CKD. This simple, inexpensive and harmless marker can help clinicians identify patients most likely to have CAD and decide treatment in accordance. Funding Acknowledgement Type of funding sources: None.

The prognostic value of monocyte/ high-density lipoprotein ratio in chronic heart failure

Abstract Background The monocyte-high density lipoprotein ratio (MHR) is an inflammation marker that combines monocyte counts, increased in inflammatory states, and high-density lipoprotein (HDL) levels, that appear to have protective and anti-inflammatory properties. It has been studied in chronic kidney disease, primary hypertension, cerebrovascular disease and coronary artery disease (CAD), and has been shown to be positively correlated with disease severity and worse prognosis. Although inflammation is thought to be implicated in heart failure's (HF) pathophysiology, little is known about the role of MHR in the HF setting. Purpose To study the prognostic impact of MHR in chronic HF. Methods We conducted a retrospective cohort study in ambulatory patients with HF with left ventricular systolic dysfunction (LVSD) that were followed in our HF clinic from January/2012 to May/2018. Patients with no data on monocyte counts or HDL levels in the first appointment were excluded. Endpoint under analysis: all-cause mortality. Patients were categorized according to MHR quartiles: 1st quartile <11, 2nd quartile ≥11 to <15, 3rd quartile ≥15 to <20 and 4th quartile ≥20. A Cox-regression analysis was used to assess association between MHR and all-cause mortality. A multivariate model was built adjusting for age, sex, hypertension, diabetes mellitus (DM), CAD, obstructive sleep apnea, inflammatory/autoimmune disease, atrial fibrillation, anaemia, renal dysfunction, brain-type-natriuretic peptide (BNP), New York Heart Association (NYHA) class, low-density lipoprotein value, prognostic modifying therapy and severe LVSD. Results We studied 560 chronic HF patients with LVSD, mean age 70±12 years, 67.5% men, 37.1% in NYHA class I, 44.8% in NYHA class II and the remaining in higher classes. Patients with MHR≥20 (last quartile) were mostly men with higher prevalence of DM and CAD, they more often presented in higher NYHA classes and with worse renal function and higher BNP. No difference existed concerning doses of evidence based-drugs. During a median follow-up of 53 (32–88) months from the index medical appointment 256 patients (45.7%) died. Mortality was similar in the lower three MHR quartiles, being statistically higher in the last quartile. Considering only two groups - MHR <20 and MHR ≥20 - the all-cause mortality rate was of 41.6 vs. 57.6%, respectively, p=0,001. MHR was independently associated with poor survival. In patients presenting with MHR ≥20, the HR of long-term all-cause mortality was 1.42 (95% CI: 1.04–1.93), p=0.03 when compared with those with lower MHR. Conclusions Chronic HF patients with MHR ≥20 have a significant 42% increased risk of long-term all-cause death. We reinforce the role of inflammation in chronic HF. The MHR is a practical, inexpensive and widely available parameter that can help clinicians in the identification of chronic HF patients at higher risk of death. Funding Acknowledgement Type of funding sources: None.

The association of stroke rate with low density lipoprotein and statin exposure in patients with atrial fibrillation (AF)

Abstract Background There are limited data on the association between cholesterol levels and stroke risk in atrial fibrillation (AF). Objective To quantify the association of stroke rate in AF with low-density lipoprotein (LDL) levels and statin use. Methods Using linked administrative databases in Ontario, Canada, we conducted a population-based retrospective cohort study of patients aged ≥66 years, diagnosed with AF between 2009–2019. We used cause-specific hazard regression to determine the association of statin use with stroke rate. We developed a second cause-specific regression model for patients with at least one lipid profile measurement in the year before AF diagnosis to study the association of LDL levels with stroke rate, while adjusting for statin use. LDL levels were modeled using restricted cubic splines (RCS). Both models were adjusted for age, sex, heart failure, hypertension, diabetes, stroke or transient ischemic attack, and vascular disease at baseline, plus anticoagulation as a time-varying covariate. Results We studied 261,659 qualifying patients (median age 78 years, 49% female), of whom 3,954 (1.5%) developed a stroke during one-year follow-up. A total of 142,834 (54.6%) patients were treated with statins and 145,775 (55.7%) had lipid measurements before AF diagnosis. The adjusted RCS analyses (see Figure) indicated increasing hazard ratios (HRs) for stroke with increasing low-density lipoprotein (LDL) values above 1.5mmol/L. Statin use was associated with a lower stroke rate relative to non-users (hazard ratio 0.87, 95% confidence interval 0.81–0.93, p-value <0.0001). Conclusion LDL levels above 1.5mmol/L were independently associated with higher stroke rates in patients with AF, while statins were associated with lower stroke rates independent of anticoagulation. This suggests that LDL measurements may improve stroke risk stratification in AF, while statins may offer an underutilized pathway to lower stroke risk in AF. Funding Acknowledgement Type of funding sources: Foundation. Main funding source(s): 1) Heart and stroke foundation of Canada HR for Stroke and LDL level

Effect of Spirulina platensis green-blue algae consumption, and circuit resistance training (CRT) on lipid profile in overweight and obese middle-aged men

Background and Aims: Researchers have recently considered the impact of herbal supplements combined with exercise on weight control. This study aimed to investigate the effect of Spirulina platensis green-blue algae consumption, and circuit resistance training (CRT) on lipid profile in overweight and obese middle-aged men. Materials and Methods: This was a single-blind, quasi-experimental, and applied study. The study population included 60 overweight and obese men in the age range of 30-55 years with a body mass index of more than 25 (kg/m2). The participants were purposefully selected and randomly divided into four groups of training plus spirulina (N=15); training plus placebo; spirulina; and Placebo. The intervention and placebo groups took two 500 mg spirulina capsules, and placebo capsules daily for eight weeks, respectively. The CRT was performed for eight weeks and three sessions per week based on a flexible timing pattern, with the observation of the principle of overload with rest intervals between stations and sets. Results: There was a significant difference between the study groups in terms of the levels of triglyceride (TG), high-density lipoprotein (HDL) of the participants (P<0.05). Moreover, in the intra-group investigation, there was a significant reduction in the values of low-density lipoprotein (LDL), and triglyceride (TG), in the groups of training plus spirulina and training plus placebo, cholesterol (TC), and (Aspartate aminotransferase) AST in the groups of training plus spirulina had a significant decrease, Moreover, a significant increase was observed in the levels of HDL and alanine aminotransferase (ALT) in the group of training plus spirulina. Conclusion: It seems that the consumption of Spirulina platensis green-blue algae combined with CRT can be effective in controlling weight and obesity and preventing obesity-related diseases through the improvement of some indices of metabolic profile and body composition.

Вивчення ефективності препарату Вазонат у комплексному лікуванні хворих на цукровий діабет 2-го типу

Мета дослідження — вивчення ефективності Вазонату (мельдонію) у хворих на цукровий діабет 2-го типу у порівняльному 12-тижневому дослідженні. Матеріали та методи. Обстежено 55 хворих на цукровий діабет (ЦД) 2-го типу. Пацієнтам першої групи на додаток до базисної терапії ЦД призначали мельдоній (Вазонат, АТ «Олайнфарм», Латвія) в дозі 1000 мг/добу впродовж 12 тижнів. До другої групи увійшло 25 хворих на ЦД 2-го типу (8 чоловіків і 17 жінок, середній вік 60,4 ± 1,9 року), які отримували тільки базисну терапію ЦД. Результати. 12-тижнева терапія мельдонієм у складі комбінованої терапії ЦД 2-го типу статистично значимо покращує показники функціонального стану нирок, на що вказує значуще зниження рівня мікроальбумінурії, креатиніну та збільшення швидкості клубочкової фільтрації в першій групі пацієнтів. Включення в схему лікування Вазонату у хворих на ЦД 2-го типу покращує морфофункціональні параметри серця, має додаткову нефропротекторну дію, знижує середні значення рівня тригліцеридів і ліпопротеїнів низької щільності, а також рівень глікованого гемоглобіну порівняно з базисною терапією. Висновки. Вазонат, що поєднує кардіопротективні властивості та цукрознижуючий ефект, є особливо вдалим вибором для комплексної терапії пацієнтів із порушеннями метаболізму глюкози, діабетичною нефропатією і серцевою недостатністю.

Impact of Primary Care Usual Provider Type and Provider Interdependence on Outcomes for Patients with Diabetes

Research ObjectiveQuality of diabetes care delivered to patients with different types of usual providers of care [i.e., physician, physician assistant (PA) or nurse practitioner (NP)] is similar. However, primary care (PC) providers often provide care to each other's patients (i.e., “share” common patients). The impact of patient sharing, or interdependence, is on quality of diabetes care is unknown. As a result, some providers and organizations hesitate to formalize patient sharing by creating multi‐provider teams due to concerns about the impact of impact of such teams on quality of care. We sought to both 1) evaluate the association of usual provider type (physician or PA/NP) provider and 2) interdependence on outcomes for patients with diabetes.Study DesignThis patient‐level cohort study used electronic health record data from 24 health system‐affiliated PC practices in central North Carolina. Patients' usual PC provider was the provider most frequently seen during 2016 and 2017. Patient‐level independent variables included demographic, medical complexity, and healthcare utilization (separate variables for PC, specialty, emergency department, and hospital). Provider panel‐level variables [usual provider of care type (physician or PA/NP), panel size, and provider interdependence (# shared patients / # supplemental providers then categorized into quartiles)]. We examined the association of diabetes quality (at least two hemoglobin A1c (HbA1c) tests, at least one low‐density lipoprotein (LDL) cholesterol test, mean HbA1c and LDL values) during 2017 with all variables simultaneously using logistic or linear regression with clustering by practice.Population StudiedAdults with diabetes (N = 10,498) on 131 panels (physician = 111; PA/NP = 20).Principal FindingsNinety percent of patients had physicians as usual providers (N = 9462). Patient demographics, complexity and utilization were similar for patients of different usual provider types except for mean age (physician = 64.6; PA/NP = 59.7) and insurance type (% Medicaid/uninsured: physician: 5.5; PA/NP: 11.2). Most patients had at least two HbA1c tests (72%) and one LDL test (65%). Average HbA1c (7.5 mmHg) and LDL (109 mg/dL) was also similar by usual provider type. Average panel size was 80 diabetes patients (physician = 85; PA/NP = 52) Panels had a mean interdependence of 6.1 patients/supplemental provider (physician = 6.2; PA/NP = 5.8). There were no statistically significant differences in HbA1c or LDL testing by usual provider type or interdependence. Similarly, there was no statistically significant difference in HbA1c for mean HbA1c values by usual provider type or interdependence. However, increases in interdependence quartile resulted in increases in mean LDL values (β = 13.8, p = 0.016).ConclusionsThe quality of diabetes care does not differ based on provider type, either PA/NPs or physicians. Increases in provider interdependence (greater numbers of patients per provider) resulted in higher mean LDL values, but only the interdependence values in the highest quartile would result in a mean LDL in the borderline high range.Implications for Policy or PracticeOrganizations using or considering interdependent, multiple‐provider teams will likely not see a reduction in diabetes‐specific quality metrics. However, additional patient and provider outcomes should be evaluated, such as delivery of preventive services, outcomes important to patients with multiple chronic conditions, and provider satisfaction and burn‐out.Primary Funding SourceNational Institutes of Health.

Assessment of hypertension association with arsenic exposure from food and drinking water in Bihar, India

Epidemiological studies have associated chronic exposure to arsenic (As) from drinking water with increased risk of hypertension. However, evidence of an association between As exposure from food and hypertension risks is sparse. To quantify the association between daily As intake from both food (rice, wheat and potatoes) and drinking water (Aswater) along with total exposure (Astotal) and hypertension risks in a study population in Bihar, India, we conducted an individual level cross-sectional analysis between 2017 and 2019 involving 150 participants. Arsenic intake variables and three indicators of hypertension risks (general hypertension, low-density lipoprotein (LDL) and high-density lipoprotein (HDL)) were derived, and any relationship was quantified using a series of crude and multivariable log-linear or logistic regression models. The prevalence of general hypertension was 40% for the studied population. The median level of HDL was 45 mg/dL while median value of LDL was 114 mg/dL. Apart from a marginally significant positive relationship between As intake from rice and the changes of LDL (p-value = 0.032), no significant positive association between As intake and hypertension risks could be ascertained. In fact, Astotal was found to be associated with lower risks of general hypertension and higher levels of HDL (p-value = 0.020 and 0.010 respectively) whilst general hypertension was marginally associated with lower Aswater (p-value = 0.043). Due to limitations regarding study design and residual confounding, all observed marginal associations should be treated with caution.

Study to find out the significance of postprandial dyslipidemia in diabetic patients

: Diabetes Mellitus (DM) is a group of metabolic diseases, which is characterized by chronic hyperglycaemia, which results from the defects in the insulin action, insulin secretion or both. The most prevalent form of the disease, type 2 Diabetes Mellitus is often asymptomatic in the early stages and it may remain undiagnosed for many years.The insulin resistance in the liver leads to failure of the hyperinsulinaemia to suppress the gluconeogenesis, which increases fasting glucose levels and decreases. glycogen storage by the liver in the postprandial phase. Increased glucose production in the liver occurs early in the course of diabetes, and it is likely in skeletal muscles after the onset of the insulin secretory abnormalities and the insulin resistance. Due to the insulin resistance in the adipose tissue and obesity, the free fatty acid (FFA) flux from the adipocytes is increased, which in turn leads to an increase in lipid [very low-density lipoprotein (VLDL) and triglycerides] synthesis in the hepatocytes. This is responsible for the dyslipidaemia which is found in type2 diabetes mellitus [elevated triglycerides, reduced HDL, and increased low-density lipoprotein (LDL) particlesIndividuals with type 2 diabetes mellitus are at increased risk of developing microvascular and macrovascular complications.: To find out the significance of postprandial dyslipidemia in diabetic patients.: This is a cross-sectional study, wherein written informed consent was taken after giving detailed information to the participants regarding the study. Patients who were in the age group of 35-65 years, admitted in the Department of Medicine, RRMCH from November 2017 for next 18 months with Diabetes Mellitus who met a predefined inclusion and exclusion criteria were studied. The study was initiated after obtaining clearance from the institution's ethical committee.: There was a significant elevation of mean values of total serum cholesterol, LDL and TG of cases and controls in the postprandial state compared to their fasting state, statistical significance was found (P<0.05).Mean values of total serum cholesterol, LDL and TG of cases in fasting state were significantly more compared to controls in fasting state, statistical significance was found (P<0.05).Mean values of total serum cholesterol, LDL and TG of cases in the postprandial state were significantly more compared to controls in the postprandial state, statistical significance was found (P<0.05).Mean values of both FBS and PPBS in cases were higher compared to controls (P<0.05). Mean values of PPBS in cases and controls were significantly more compared to their respective FBS values (P<0.05).The mean HbA1c values in cases were higher(7.142) compared to controls (5.554).: Prevalence of diabetes was highest in the age group 56-65 years in our hospital.As the duration of diabetes increases, there is an increased prevalence of dyslipidemia in the cases.Past history of HTN, IHD, PVD and CVA were found significantly more in subjects with fasting and postprandial dyslipidemia (cases) compared to those without(controls).Patients on irregular treatment (63%) were more in the study group(cases) compared to controls. So it could be said that patient not on regular treatment are more prone to have dyslipidemia. (fasting as well as postprandial). There was a significant increase in total serum cholesterol, LDL and TG in postprandial states of cases compared to that in controls, so it could be said that diabetic patients with fasting dyslipidemia are more prone to have dyslipidemia in the postprandial state.

Physiological Correlation between Hypothalamic-Pituitary-Adrenal Axis, Leptin, UCP1 and Lipid Panel in Mares during Late Pregnancy and Early Postpartum Period.

Simple SummarySeveral features of periparturient mares’ physiology have been investigated; however, the possible linkage between HPA axis, leptin, and lipid metabolism in periparturient mares has received little attention. Therefore, the present study aimed to investigate the dynamic changes of adrenocorticotrophic hormone, cortisol, leptin, UCP1, lipids and liproteins levels in mares during late pregnancy and the postpartum period. The findings obtained in the current survey showed a dynamic modulation of studied parameters, namely the response to hormonal and metabolic adaptations, occurring during the peripartum period in mares. This study suggests an interaction of the HPA axis with lipid metabolism in mares, probably to deal with the metabolic load occurring during pregnancy and with lactation energy supplies.This study aimed to investigate the dynamic change of adrenocorticotrophic hormone (ACTH), cortisol, leptin, mitochondrial uncoupling protein 1 (UCP1), lipids and lipoproteins in mares during late pregnancy and the postpartum period. A total of 20 mares (10 pregnant mares, monitored from 14 ± 2 days before expected foaling until 14 days after foaling, Group A; 10 non-pregnant and non-lactating mares, Group B) were enrolled in the study. Body Condition Score (BCS) and body weight (BW) values were recorded from each animal. In Group A, blood samples were collected on days 14 ± 2 and 7 ± 2 before foaling (T−14; T−7), and on days 7 and 14 after foaling (T+7; T+14). From mares of Group B, blood samples were collected at the beginning of the study. The levels of ACTH, cortisol, leptin, UCP1, non-esterified fatty acids (NEFAs), total cholesterol, high-density lipoproteins (HDLs), low-density lipoproteins (LDLs), triglycerides and very-low-density lipoproteins (VLDLs) were investigated. While BCS showed no statistical change throughout the monitoring period (p > 0.05), all the other studied parameters displayed statistically significant variations in Group A over the peripartum period (p < 0.0001). A significant effect of pregnancy was found on all studied parameters (p < 0.001). The ACTH and cortisol levels measured in mares belonged to Group A showed a significant positive correlation with the values of leptin, LDLs, triglycerides and VLDLs, whereas they were negatively correlated with the serum UCP1 and NEFAs values. Together, the findings gathered in this study highlight a dynamic change of serum leptin, UCP1 and lipid parameters in peripartum mares and suggest an interaction of the HPA axis with lipid metabolism and mobilization in mares during the peripartum period in order to deal with metabolic and energy demand and maintain energy homeostasis.

Simvastatin reverses the harmful effects of high fat diet on titanium rod osseointegration in ovariectomized rats.

The objectives of the present study were to determine whether simvastatin (SIM) could reverse the harmful effects on titanium rod osseointegration in ovariectomized rats fed high-fat diet (HFD). Ovariectomized female Sprague-Dawley rats were randomly allocated to three groups and received SIM treatment plus HFD for 12 weeks. We then evaluated the microstructure parameters, histological parameters, biomechanical parameters, bone turnover, and blood lipid level. After 12 weeks of treatment, SIM can significantly improve bone formation around the titanium rod and osseointegration including higher values of maximum push-out force, bone area ratio (BAR), bone-to-implant contact (BIC), bone mineral density (BMD), bone volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), mean connective density (Conn.D) when compared with the HFD group. In addition, system administration of SIM showed positive effects on collagen type 1 cross-linked C-telopeptide (CTX-1), procollagen I N-terminal propeptide (PINP), total cholesterol (TC), triglycerides (TGL), low-density lipoprotein (LDL) cholesterol and high-density lipoprotein (HDL) cholesterol. Compared with the HFD group, lower values of CTX-1, P1NP, TC, TGL and LDL were observed in the SIM+HFD group (P < 0.05). Our findings revealed that HFD may have an adverse effect on osseointegration in osteoporotic conditions, and the harmful effect of HFD on osseointegration could be reversed by SIM.

Effect of Calamari Oil on Lipid Profile of Streptozotocin Induced Diabetic Rats

Aim: The aim of this study was to assess the effect of Calamari Oil on lipid profile levels in diabetes streptozotocin induced diabetic Rats.&#x0D; Study design: An experimental study.&#x0D; Place and duration of study: Animal House, Department of Applied and Environmental Biology, Rivers State University, Port Harcourt and University of Port Harcourt Rivers State, Nigeria, between February 2020 and August 2020.&#x0D; Methodology: Thirty Six (36) albino rats were purchased and allowed to acclimatize for two (2) weeks in the laboratory at the animal farm house of the Department of Animal and Environmental Biology, Rivers State University. They were fed the normal rat feed (Chow feed) and water was allowed ad libitum. The rats were weighed and randomly grouped into six (6) groups with six rats in each group. Group 1 (Negative control) was placed on normal diet while groups 2 to 6 were placed on a high fat diet (HFD) prior to the induction with Streptozotocin to achieve diabetes and the animals were treated according to their groupings for four weeks by means of oral gavage. The dose of Calamari Oil administered to the rats was extrapolated from human doses. The high fat diet was prepared by mixing the animal feed (Chow diet) with margarine in a ratio of 3:1. After each period of treatments, blood samples were collected from the rats at the end of the treatments via cardiac puncture by anaesthetizing the rats with chloroform after a six (6) hour fast. Fasting blood glucose was determined using the Glucose Oxidase method, lipid profile was analysed spectrophotometrically and Atherogenic coefficient (AC) and Castelli ratio index-1 level (CRI-1) were calculated. The GC–MS analysis of bioactive compounds from Calamari Oil was done using Agilent Technologies GC systems with GC-7890A/MS-5975C model. Data generated were analysed using SPSS version 22.0 of windows statistical package. Results were considered statistically significant at 95% confidence interval (p &lt; 0.05).&#x0D; Results: The results showed that after week 1 - 4 of exposure, the mean TG (Triglyceride) value of the Negative control group (NC), Positive control (PC) group, diabetic groups exposed for weeks 1, 2, 3 and 4 expressed in mg/dl were 130.89 ± 2.52, 174.94 ± 3.11, 166.64 ± 1.95, 160.61 ± 0.60, 153.37 ± 2.24 and 141.62 ± 0.99 respectively. Mean TC (Total cholesterol) value of the NC, PC group, diabetic groups exposed for weeks 1, 2, 3 and 4 expressed in mg/dl were 160.93 ± 2.99, 194.96 ± 2.09, 188.18 ± 1.41, 180.63 ± 0.59, 169.96 ± 1.47 and 159.71 ± 1.43 respectively. HDL (High density lipoprotein) value of the NC, PC group, diabetic groups exposed for weeks 1, 2, 3 and 4 expressed in mg/dl were 83.05 ± 2.96, 65.68 ± 1.78, 66.97 ± 1.28, 72.75 ± 0.52, 76.35 ± 0.77 and 78.94 ± 0.86. Mean LDL (Low density lipoprotein) value of the NC, PC group, diabetic groups exposed for weeks 1, 2, 3 and 4 expressed in mg/dl were 51.7 ± 3.21, 94.3 ± 0.48, 88.15 ± 1.08, 75.76 ± 0.62, 62.95 ± 0.94 and 52.46 ± 0.59 respectively. The mean non- HDL cholesterol value of the NC, PC group, diabetic groups exposed for weeks 1, 2, 3 and 4 expressed in mg/dl were 159.93 ± 2.99, 193.96 ± 2.09, 187.18 ± 1.41, 179.63 ± 0.59, 168.96 ± 1.47 and 158.71 ± 1.42 respectively. Also, mean cholesterol ratio value of the NC, PC groups, diabetic groups exposed for weeks 1, 2, 3 and 4 expressed in mg/dl were 1.94 ± 0.07, 2.97 ± 0.06, 2.81 ± 0.04, 2.48 ± 0.02, 2.22 ± 0.01 and 2.02 ± 0.01respectively.&#x0D; Conclusion: Type 2 diabetes is associated with dyslipidemia and as such the treatment with the Calamari Oil in the treated groups had positive effect on lipid profile markers with TG, TC, LDL, AC, CRI-1 decreasing and HDL increasing with increase in duration of time.

Comparative Analysis between Martin’s Formula and Friedewald’s Formula with Direct Homogenous Assay for Estimating Low Density Lipoprotein Cholesterol Level in Nepalese Population

Background: Low density lipoproteinforms a basis of decision making in treatment of hypercholesterolemic patients and primary target of intervention. Its cost effective and accurate measurementis a need for every clinical laboratories and different calculation methods has been adopted as a replacement to direct assays. This study aims to evaluate the Martin’s formula and Friedewald’s formula in a sample of Nepalese population compared against direct homogenous assay.&#x0D; Materials and Methods: This is a cross-sectional study conducted in Department of Biochemistry from Feb 2020 to January 2021. Serum samples of the participants were analysed for total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein. Martin’s and Friedewald’s formula were applied to get calculated value of low density lipoprotein from both methods. Passing and Bablok regression analysis was used for methods comparison.&#x0D; Results: The mean age of participants was 54.2 ± 8.9 years. Passing-Bablok regression analysis showed Friedewald’s formula performed better than Martin’s formula as per systematic and proportional bias when compared with directassay. However at lowerserum low density lipoprotein level, underestimation of low density lipoprotein compared to direct assay was more common in Friedewald’s formula. At high triglyceride level more percentage error of difference of mean from direct assay was found for Friedewald’s formula.&#x0D; Conclusion: When compared to direct assay, Friedewald’s formula was found to be in better agreement than Martin’s formula. Martin’s formula had advantage over Friedewald’s formula at lower serum low density lipoprotein level and higher triglyceride level where Friedewald’s formula mostly underestimated low density lipoprotein.

Uncoupling Protein-1 (UCP1) in the Adult Horse: Correlations with Body Weight, Rectal Temperature and Lipid Profile.

Simple SummaryUncoupling protein-1 (UCP1) plays important roles in the energy balance and regulation of metabolism and in the body temperature regulation. In this survey the correlation among UCP1, body weight, rectal temperature and lipid profile was assessed in the adult horse. The findings gathered from the current survey showed that UCP1 values are not related with body weight and temperature in studied animals, but they seem to be linked to pathways involved in lipid and lipoprotein metabolism.This study aimed to evaluate the possible relationship among UCP1, body weight, rectal temperature and lipid profile in the horse. Thirty clinically healthy Italian Saddle geldings (6–10 years old) were enrolled after the informed owners’ consent. All horses were blood sampled and their body weight and rectal temperatures were recorded. On the sera obtained after blood centrifugation the concentration of UCP1, total lipids, phospholipids, non-esterified fatty acids (NEFAs), triglycerides, total cholesterol, high density lipoproteins (HDLs), low density lipoproteins (LDLs) and very low density lipoprotein fraction (VLDLs) was evaluated. Pearson’s correlation analysis was applied to assess the possible relationship between serum UCP1 concentration and the values of body weight, rectal temperature and lipid parameters. Serum UCP1 concentration showed no correlation with body weight, rectal temperature, HDLs and LDLs values, whereas it correlated negatively with serum total lipids, phospholipids, NEFAs, total cholesterol, triglycerides and VLDLs values (p < 0.0001). The findings suggest that in the adult horse the role of UCP1 is linked to the lipid metabolism rather than to thermoregulation.

Multicomponent intervention provided by GPs to reduce cardiovascular risk factors: evaluation in an Italian large sample.

The cardiovascular risk increases in a multiplicative way when patients present more risk factors simultaneously. Moreover, the General Practitioners (GPs) play a crucial role in risk factors prevention and reduction. This work aimed to evaluate a multicomponent intervention in the Primary Care Department in an Italian Local Health Unit. A pre-post study was conducted in Northern Italy (2018). Patients were eligible if: aged between 30 and 60 years, not chronic patients, not affected by hypertension or hypercholesterolaemia. The GPs assessed body mass index, hypertension, abdominal obesity, low-density lipoprotein (LDL) values, glycaemic values, smoking and exercise habit (T0). A counselling by GPs to at-risk patients and a multicomponent health education intervention were performed. Reassessment occurred after at least 3 months (T1). Main analyses were chi-squared tests for gender differences, McNemar or marginal homogeneity tests for changes in paired data (P < 0.05 as significant). Participants were 5828 at T0 (54.0% females) and 4953 at T1 (53.4% females). At T0, 99.1% presented at least one risk factor. Significant changes in paired data were reported for each risk factor. The greatest improvement frequencies occurred in glycaemia values (51.0%) and hypertension (45.6%), the lowest in abdominal obesity (3.7%). Some differences were recorded between genders, e.g. females reported higher improvement frequencies in hypertension (P = 0.001) and abdominal obesity (P < 0.001), whereas males in physical activity (P = 0.011) and LDL values (P = 0.032). The results showed significant changes for each risk factor, both for men and women. GPs and multicomponent educational interventions could play a key role in reducing cardiovascular risk factors.

Lipid Profile Response to Electroacupuncture in Non-Alcoholic Fatty Liver Patients with Hyperlipidemia.

Non-alcoholic fatty liver disease (NAFLD) is estimated to be one of the most common diseases affecting the liver because of its high prevalence worldwide. Abnormal lipid profile between NAFLD patients has been reported in several studies. This study aimed to evaluate the lipid profile response to electroacupuncture in NAFLD patients. A total of 60 female patients with NAFLD were included in the study with ages ranged from (30-55) years old. They were divided equally into two groups, group A received electroacupuncture (EA) stimulation at points of; LR14, LR3, ST36, and GB34. And group B received sham acupuncture application in non-acupuncture points. The demographic data and lipid profile such as total cholesterol (TC), serum triglycerides (TG), serum high-density lipoprotein (HDL), serum low-density lipoprotein (LDL) were recorded before and after the study. The study results revealed a significant decrease (p < 0.05) of LDL, TC, TG after 6 weeks of non-interrupted treatment sessions in group A, However HDL showed no significant improvement (p > 0.05). A significant difference was found between posttreatment values of LDL, TC, and TG between both groups. Electroacupuncture can be an effective, simple, and applicable method for the improvement of elevated lipid profiles in NAFLD patients.

English

BACKGROUND Evaluation of endothelial function in isolated coronary artery ectasia (ICAE) with flow-mediated dilation (FMD) is limited and use of flow mediated vasodilation as a surrogate marker for the extent of coronary atherosclerosis remains unknown. Thus, the following study was done to evaluate for endothelial dysfunction (ED) in subjects with ICAE by assessing the FMD in the brachial artery. METHODS This was an observational study, conducted at a referral hospital providing tertiary care in India between June 2017 and November 2018. Fifty patients with ICAE and fifty control patients with normal coronary arteries (NCA) on coronary angiogram, done by using a standard Seldinger technique via femoral route, were the subjects of the study. Brachial artery FMD was determined by using a highresolution ultrasound system (Samsung RS80A) using a linear transducer of a frequency of 7.5 Mega Hertz. RESULTS The groups had same baseline characteristics in terms of age, sex etc. (all P values &gt; 0.05). However, values of serum uric acid and low-density lipoproteins were statistically significantly higher in ICAE subjects (P &lt; 0.0001). Whereas, dilatation of brachial artery in response to shear stress was significantly lower in patients with ICAE subjects than in patients with normal coronaries. (8.85 ± 0.49 vs. 12.83 ± 0.45, P &lt; 0.0001). Marki’s classification, type 1, type 2, type 3 and type 4 coronary artery ectasia (CAE) was present in 8 (16 %), 4 (8 %), 13 (26 %), and 25 (50 %), respectively. CONCLUSIONS Atherosclerosis may play a pivotal role in the genesis of ICAE. Hyperlipidaemia may have a specific role in the disease process. Further research is required to evaluate the exact molecular mechanisms involved in CAE. KEYWORDS Coronary Artery Disease, Ectasia, Endothelial Dysfunction, Hyperlipidaemia