δ15N Values Research Articles

Enantiomer-Specific Stable Carbon and Nitrogen Isotopic Analyses of Underivatized Individual l- and d-Amino Acids by HPLC + HPLC Separation and Nano-EA/IRMS.

We developed a new method for stable carbon and nitrogen isotopic (δ13C and δ15N) analysis of underivatized amino acid (AA) enantiomers simultaneously, based on high-performance liquid chromatography (HPLC) separation and off-line isotopic measurement. l- and d-Enantiomers of each AA were isolated using a ReproSil Chiral-AA column, purified by wet chemical procedure, and analyzed for δ13C and δ15N values with a nanomol-scale elemental analyzer/isotope-ratio mass spectrometry (nano-EA/IRMS) system. We successfully achieved the separation of l- and d-enantiomers of 15 proteinogenous AAs, with all l-enantiomers eluting before respective d-enantiomers. The δ13C and δ15N values of AA enantiomers were consistent before and after HPLC separation, demonstrating that this analytical method conserves isotopic information. By coupling this column with a multidimensional HPLC system for isolating individual AAs, we analyzed l- and d-AAs in a natural sample, peptidoglycan isolated from Gram-positive bacterium Bacillus subtilis. Results show a surprisingly large 15N-depletion, up to 20‰, in d-glutamic acid relative to its l-counterpart. The first example, to our knowledge, of δ13C and δ15N analyses of underivatized AA enantiomers is expected to contribute to various research areas in the future.

First direct measurements of the nitrogen isotopic composition of vehicle tailpipe-emitted NH3 and implications for the source apportionment of NH3 in urban atmosphere.

The introduction of three-way catalytic converter (TWC) to meet stringent vehicular NOx emission standards worldwide has led to an unintended consequence of vehicle-derived ammonia (NH3) emission, which might degrade air quality and affect human health, especially in urban areas. The nitrogen stable isotope composition of NH3 (δ15N-NH3) may be a useful tool to trace NH3 sources, but the isotopic signature of vehicle-emitted NH3 is lacking. Here we report the δ15N-NH3 measured from tailpipe exhausts collected directly from 19 different vehicles equipped with TWC using "grab" sample technique optimized to avoid isotopic fractionation. We found a large of range of NH3 concentration (from 1 to 37 ppm, x̄ ± 1σ = 10 ± 8 ppm) and δ15N value (from -38.1 ‰ to 49.0 ‰, x̄ ± 1σ = 1.2 ± 20.9 ‰) for our samples (n = 57). Our results indicate a correlation between δ15N-NH3 and NH3 concentrations (i.e. δ15N-NH3 (‰) = 16.9(± 2.2)In(NH3 concentration; ppm) - 31.7(± 4.7) (R2 = 0.53, P < 0.01) that is associated with the catalyst temperature, which is generally agree with the results of theoretical calculation. After consideration of the vehicle emission evolution, dominant driving condition and tunnel δ15N-NH3 variation, a representative δ15N-NH3 value (meanminmax) of vehicle source is identified as 0.8000-2.20005.2000, which is distinct from (larger than) other NH3 volatilization-related emission sources. Using our newly measured δ15N values and Bayesian mixing model, we approximate the overall contribution of vehicles to ambient NH3 before, during, and after an international event in Beijing was 14.1 %, 7.9 %, and 21.5 %, respectively, which agree well with the course of traffic restriction. Collectively, our findings demonstrate the importance of using vehicle-emitted δ15N-NH3 to quantify vehicular NH3 contribution in urban atmospheres.

Stable Isotopes Analysis of Black Lion Tamarins Reveals Increasing Arthropod Consumption When Fruit Productivity Decreases in Forest Fragments.

Given the cryptic and elusive nature of prey consumption, quantifying its contribution to the diet of free-ranging primates using behavioral methods is challenging. In this context, the use of carbon and nitrogen-stable isotopes represents a promising alternative approach. Here, we used stable isotope analysis to estimate the proportion of arthropods and fruits in the diet of black lion tamarins (Leontopithecus chrysopygus), an endangered primate endemic to the Brazilian Atlantic Forest. To do so, we ran stable isotope mixing models using isotopic data from hair samples of black lion tamarins living in six forest fragments showing different levels of habitat quality. Furthermore, we ran linear mixed models to assess the influence of habitat quality-fruit productivity (estimated by tree total basal area) and arthropod biomass - and individual characteristics (sex and body mass) on tamarins' δ15N values (a proxy for trophic position). Our results revealed that arthropods contributed more to black lion tamarins' diet than reported in previous behavioral studies, suggesting that behavioral observations may considerably underestimate the importance of arthropodivory in the diet of arboreal primates. The degree of arthropodivory and frugivory was similar within groups, in line with the strong group cohesion and synchronization of feeding behaviors of this species and supporting the role of site-specific habitat characteristics on dietary choice. Arthropod consumption was higher in areas with lower fruit productivity and did not increase when arthropod biomass increased, suggesting that fruits represent a limiting but preferred resource for this species. These results demonstrate the dietary plasticity of black lion tamarins in areas of lower forest quality, where they manage to compensate low fruit productivity by shifting to a diet richer in arthropods. Considering that this species occurs within a highly fragmented landscape, preserving and protecting small forest patches remains crucial for the conservation of this species.

Just Hitching a Ride: Stable Isotopes Reveal Non-Feeding Behaviour of Anisakis simplex Within Its Host Fish.

Anisakis simplex larvae, commonly found in marine fish, cause anisakiasis in humans, resulting in gastric to gastro-allergic symptoms. Despite known health risks, the impact of Anisakidae larvae on fish hosts is less understood. This study aimed to investigate this interaction by assessing the feeding strategy of A. simplex. Anisakis larvae were isolated from North Sea Merluccius merluccius tissues (stomach, body cavity, liver and muscle) and were analysed for carbon (δ13C) and nitrogen (δ15N) isotope values. Significant differences in δ13C values were found among host tissues, with the liver differing from muscle and stomach tissues. In contrast, no differences were noted for the associated parasites. Additionally, δ15N values indicated that the host occupied a significantly higher relative trophic position than its parasite. This suggests a lack of direct nutrient transfer from host to parasite, as the parasite would typically exhibit higher stable isotope values than the tissue they feed on. Therefore, A. simplex's stable isotope values might reflect those of its previous host (crustacean and/or small fish), providing insights into diet and movement of the paratenic M. merluccius host. Further research is needed to confirm these findings across different fish species and to explore A. simplex as a proxy for trophic ecology.

Trophic ecology, based on stable isotope values, of long-finned pilot whales Globicephala melas stranded on the Icelandic coast

In Iceland, occurrences of long-finned pilot whales Globicephala melas and strandings in coastal waters are irregular and thought to be related to prey movements; however, little is known about the population’s trophic ecology, making it difficult to assess its role in the regional ecosystem. To investigate the trophic niche of the species, we measured δ13C and δ15N values from skin and muscle samples collected from 16 strandings (12 single and 4 mass strandings) spanning 33 yr (1988-2021). We tested the effects of sex, age class and body length on δ13C and δ15N values within the species. We found that δ13C values did not differ between sexes or age classes, nor did they vary with body length. Values of δ15N did not vary with sex or body length, but varied slightly between adults and juveniles, albeit not sufficiently to support a difference in trophic levels. Whales from different mass stranding events showed similar stable isotope niche widths (approximately 0.20‰2) and overlapping isotope niches (generally &gt;45% of the standard ellipse area corrected for small sample size). Overall, pilot whales in this region showed a narrow isotopic niche width and low intraspecific variation in isotopic niche. Future analyses using complementary chemical tracers and stomach contents will be important to identify the factors driving these patterns. The first measurements of the stable isotope niche of pilot whales presented here provide a baseline for future studies to determine niche overlap between cetaceans in Iceland, contributing to their management and conservation.

Regional differences in soil stable isotopes and vibrational features at depth in three California grasslands

AbstractThere are major gaps in our understanding of how Mediterranean ecosystems will respond to anticipated changes in precipitation. In particular, limited data exists on the response of deep soil carbon dynamics to changes in climate. In this study we wanted to examine carbon and nitrogen dynamics between topsoils and subsoils along a precipitation gradient of California grasslands. We focused on organic matter composition across three California grassland sites, from a dry and hot regime (~ 300 mm precipitation; MAT: 14.6 $$\boldsymbol{^\circ{\text{C}} }$$ ∘ C ) to a wet, cool regime (~ 2160 mm precipitation/year; MAT: 11.7 $$\boldsymbol{^\circ{\text{C}} }$$ ∘ C ). We determined changes in total elemental concentrations of soil carbon and nitrogen, stable isotope composition (δ13C, δ15N), and composition of soil organic matter (SOM) as measured through Diffuse Reflectance Infrared Fourier Transformed Spectroscopy (DRIFTS) to 1 m soil depth. We measured carbon persistence in soil organic matter (SOM) based on beta ($${\varvec{\beta}}$$ β ), a parameter based on the slope of carbon isotope composition across depth and proxy for turnover. Further, we examined the relationship between δ15N and C:N values to infer SOM’s degree of microbial processing. As expected, we measured the greatest carbon stock at the surface of our wettest site, but carbon stocks in subsoils converged at Angelo and Sedgwick, the wettest and driest sites, respectively. Soils at depth (&gt; 30 cm) at the wettest site, Angelo, had the lowest C:N and highest δ15N values with the greatest proportion of simple plant-derived organic matter according to DRIFTS. These results suggest differing stabilization mechanisms of organic matter at depth across our study sites. We infer that the greatest stability was conferred by associations with reactive minerals at depth in our wettest site. In contrast, organic matter at our driest site, Sedgwick, was subject to the most microbial processing. Results from this study demonstrate that precipitation patterns have important implications for deep soil carbon storage, composition, and stability.

Mycorrhizal specialization toward each distinct Oliveonia fungus in two closely related photosynthetic Dactylostalix orchids

Abstract Although rhizoctonias from Ceratobasidiaceae, Tulasnellaceae and Serendipitaceae are typical orchid mycobionts, orchid mycorrhizal fungi exhibit vast taxonomic and ecological diversity. This diversity stems from the high specificity of orchid mycorrhizal associations and the remarkable diversity of over 28,000 orchid species. The subtribe Calypsoinae is particularly notable for its diverse mycorrhizal partnerships, including rhizoctonias, ectomycorrhizal and saprotrophic non‐rhizoctonia fungi. However, the mycobionts within certain Calypsoinae lineages, such as the genus Dactylostalix, remain understudied. This study explores the physiological ecology of two photosynthetic Calypsoinae species, Dactylostalix ringens and Dactylostalix uniflora, to gain insight into potentially novel associations and their ecological implications. We analysed the mycorrhizal communities of both Dactylostalix species using high‐throughput ITS metabarcoding of root samples collected from multiple locations. Additionally, we measured the natural abundances of 13C and 15N isotopes in the leaves of the two Dactylostalix species and their co‐occurring autotrophic reference plants, as well as in fungal pelotons isolated from D. ringens, to assess the potential for partial mycoheterotrophy. Our findings revealed that D. ringens and D. uniflora form specialized mycorrhizal associations predominantly with distinct lineages of Oliveonia (Oliveoniaceae, Auriculariales), even in sympatric populations. Stable isotope analysis showed that both Dactylostalix species exhibited conflicting isotopic signals: elevated δ15N values, supporting partial mycoheterotrophy, but lower δ13C values compared to autotrophic plants, suggesting autotrophy. Peloton samples from D. ringens displayed only modest 13C enrichment relative to autotrophic references. These conflicting isotopic signals make it difficult to precisely determine whether both Dactylostalix species are autotrophic or partially mycoheterotrophic. Intriguingly, the 13C and 15N signatures of Dactylostalix species and their pelotons resemble those of many rhizoctonia‐associated orchids. This isotopic evidence implies a niche overlap with endophytic tendencies between rhizoctonias and Oliveonia, suggesting that potential endophytic traits may have facilitated the recruitment of Oliveonia as novel mycorrhizal partners. Furthermore, the mycorrhizal segregation between D. ringens and D. uniflora likely promotes their sympatric coexistence and may contribute to reproductive isolation through ecological specialization. Read the free Plain Language Summary for this article on the Journal blog.

Nutrient availability in a freshwater-to-marine continuum: cyanobacterial blooms along the Lake Okeechobee Waterway

Water from the Lake Okeechobee watershed historically flowed south through the Everglades. Hydrologic alterations created the Lake Okeechobee Waterway, where lake water is periodically shunted east to the St. Lucie Estuary (C-44 canal) and west to the Caloosahatchee River and Estuary (C-43 canal). Within the last two decades, Microcystis blooms have developed in Lake Okeechobee and been discharged to the downstream urbanized estuaries, resulting in negative environmental and human health impacts. To better understand drivers of cyanobacterial blooms across this modified waterway, two cruises were conducted from the St. Lucie Estuary through Lake Okeechobee to the Caloosahatchee River Estuary during 2019 and 2020. Stations were sampled for environmental parameters, dissolved nutrients, chlorophyll a, cyanobacterial cell concentrations, and microcystins, as well as particulate organic matter (POM) nutrient properties. Higher ammonium (NH4+), nitrate + nitrite (NO3-), dissolved inorganic nitrogen (DIN), soluble reactive phosphorus (SRP), total dissolved phosphorus (TDP), and POM stable N isotope (δ15N) values were observed in the estuaries and Kissimmee River than in Lake Okeechobee. The nitrogen to phosphorus ratio (N:P), microcystins, and Microcystis cell concentrations were higher in Lake Okeechobee than documented over past decades. During Microcystis blooms, high NH4+, SRP, total dissolved nitrogen (TDN), TDP, and sucralose were observed with elevated algal δ15N. These results demonstrate the importance of local basin contributions, including those within the lake, to estuarine Microcystis blooms. This suggests that decreasing nutrient loading within the St. Lucie and Caloosahatchee estuaries would help mitigate these urban blooms. High POM δ15N values, NO3- concentrations, and N:P ratios in the Kissimmee River suggest that expanding urbanization north of the lake, represents an increasing human N source contributing to cyanobacterial blooms in Lake Okeechobee.

Variation of carbon and nitrogen stable isotope composition in leaves and roots of Littorella uniflora (L.) Asch. in relation to water pH and nutrient availability

In a phytotron experiment, the effects of pH variation and eutrophication on isoetids plants from soft-water lakes specifically the submerged form of Littorella uniflora (L.) Asch. was investigated by analyzing stable carbon and nitrogen isotopes (δ13C and δ15N). Conducted in late October 2020, 200 specimens from Lake Zawiad, near Gdansk, Poland, were examined over 75 days. The study tested three pH levels (∼4.5, ∼7.0, and ∼8.5) and a detailed 12-step nutrient gradient (nitrogen: 0–10 mg/l; phosphorus: 0–0.3 mg/l). The analysis focused on isotopic composition in leaves and roots, revealing that acidic conditions favored higher δ13C values (leaves: −22.67 ‰; roots: −23.23 ‰), suggesting a preference for lighter carbon forms in photosynthesis and intensive use of limited sources of CO2. The neutral pH variant showed the lowest δ13C values (leaves: −25.53 ‰; roots: −25.47 ‰), indicating less optimal conditions. δ15N values exhibited minimal fluctuation across pH levels, with slight variations in acidic and alkaline environments compared to neutral conditions. An observed decrease in δ13C across all pH levels with increased nutrients, alongside a rise in δ15N values, indicates a complex interaction between isotopic composition and environmental factors. Our findings suggest that L. uniflora shows a distinct isotopic response to varying pH levels, with higher δ13C values under acidic conditions potentially indicating enhanced CO2 uptake through a specialized carbon assimilation strategy. This highlights the species' adaptive mechanisms to environmental stressors, suggesting that the isotopic composition of aquatic vegetation can serve as a sensitive indicator of changes in lake ecosystems.

Application of stable isotopes and mineral elements fingerprinting for beef traceability and authenticity in inner mongolia of China

In recent years, the origin and safety of counterfeit meat products have raised significant concerns among consumers. Therefore, there was an urgent need to develop a new method using fingerprinting techniques for meat product traceability. This study aimed to evaluate the traceability and authenticity of beef from Inner Mongolia by measuring the δ13C and δ15N values, as well as 13 mineral elements. In the complex environment of feeding regimes and agricultural types, δ15N, δ13C, and Se were selected as characteristic variables to differentiate feeding regimes, while Fe, K, Tl, Sr, Mn, and δ13C were chosen as characteristic variables for tracing the origin of the beef. Linear discriminant analysis achieved an accuracy rate of up to 100 %. This research confirmed the effectiveness of combining stable isotopes with mineral elements in accurately determining the origin of Inner Mongolian beef and verifying the authenticity of typical feed, providing a valuable strategy for traceability.

Climatic drought and trophic disruption in an endemic subalpine Hawaiian forest bird

Overexploitation, habitat conversion, and introduced species have caused unprecedented extinctions and heavily degraded native bird populations in island ecosystems. In the Hawaiian Islands, stemming these losses has proven difficult as the highly specialized avifauna are often impacted – among other things – by poorly understood trophic disruptions as well as persistent climatic shifts. Here we investigate these dynamics by examining the trophic positions of 7 different taxa of producers and consumers across the last century in the subalpine māmane (Sophora chrysophylla) forest ecosystem on the island of Hawaiʻi. From museum collections and contemporary sampling, we analyzed the stable nitrogen (δ15N) values of producers and consumers to identify trophic and source amino acids and derive trophic enrichment constants specific to this food web. This enables us to reconstruct the diet of the palila (Loxioides bailleui), a critically endangered finch whose population recently declined 90 %. Our results show that from 1890 to 2006, the palila trophic position declined from 2.6 to 2.2, with cascading implications for its diet. Bayesian mixing model reconstructions indicate that palila trophic position changes likely arose from a 76 % decline (69.3 % to 16.6 % of diet) in the consumption of native moth caterpillars, and a 172 % increase (30.7 % to 83.4 %) in native plants. From the available ecosystem variables, exploratory Bayesian multiple regressions selected surface temperature changes, and the interactions of surface temperatures with drought and caterpillar parasitism as the primary drivers of these trophic changes. Despite the predicted increases of warming and drought, management interventions may build resiliency in this unique island ecosystem.

Growth inhibition and recovery of Pinus massoniana in Chongqing since the 1980s

Over recent decades, the forest ecosystems of southern China have been substantially affected by acid rain, a principal biogeochemical driver. Despite recent efforts to mitigate acid rain, the long-term adaptive responses of trees of varying ages remain insufficiently understood. In this study, we employed the tree-ring basal area increment (BAI), δ13C, δ15N, and δ18O values to identify patterns in growth inhibition and recovery in young and mature Pinus massoniana within acid rain-afflicted zones in China. A marked growth inhibition phase occurred from 1983 to 2010, characterized by a significant decline in BAI, particularly in mature trees. A growth recovery phase occurred from 2011 to 2020, with considerable BAI increases. Throughout the growth-inhibition phase, the intrinsic water-use efficiency (iWUE) increased, whereas δ15N levels were stable, suggesting gradual stomatal closure influenced by both acid rain and elevated CO2 levels. During this phase, mature and young trees exhibited differing strategies in response to environmental stressors. The growth recovery phase was characterized by significant reductions in both iWUE and δ15N, leading to a more closed nitrogen cycle. Structural equation modeling and correlational analyses identified SO42−, NO3−, and precipitation as key determinants of growth variations in both young and mature trees. Our findings underscore the effect of prolonged acid rain exposure on growth suppression across P. massoniana age groups, highlighting iWUE as a reliable predictor of tree growth, although age should be considered. With the ongoing reduction in acidic gas emissions, subtropical P. massoniana forests have exhibited significant resilience, highlighting rapid ecological recovery. We advocate for continued large-scale monitoring and model-based assessments of acid rain effects, emphasizing the need for long-term evaluations to comprehend the ecological resurgence and carbon sequestration potential within these ecosystems.

Stable isotope analysis of faunal remains from Bronze Age Kaymakçı, Western Anatolia

This paper presents the results of stable carbon ( δ13C) and nitrogen ( δ15N) isotopic analysis of bulk bone collagen from faunal remains from the Bronze Age site of Kaymakçı in western Anatolia. We use the isotopic values in conjunction with zooarchaeological data and contextual archaeological information to examine human-environment interactions; in particular, animal management and husbandry. Middle and Late Bronze Age agricultural systems in western and central Anatolia remain poorly understood, and this research aims to contribute to rectifying this research gap. We obtained stable isotopic values from both wild and domestic species, including deer, hare, birds, catfish, dogs, pigs, caprids and cattle. The δ13C values range from −22.0‰ to −16.9‰ and the δ15N values range from 2.3‰ to 10.3‰. For cattle, the δ13C values suggest that some specimens were subject to different management strategies, likely related to feeding or herding patterns or even the movement of animals in and around the region through some exchange mechanism. There appears to be no fixed location or strategy for the management of caprids and, furthermore, there is evidence to suggest that the herding/management areas of some caprids overlapped with those of wild animals such as deer and hare. Variation in δ15N values for pigs may indicate that whilst some animals in the sampled assemblage were free-roaming, others were penned. For the pigs and some other herbivorous domesticated species variation in δ15N values may also point to the exploitation of the wetland areas around Lake Marmara.

Monsoonal impacts on the community trophic niches in two temperate headwater tributaries across a land use continuum

Pulsed flows following heavy monsoon rain events alter riverine food webs, but their impact on headwater stream food webs across the continuum from forested canopy to open agricultural land use remains unclear. We investigated carbon and nitrogen stable isotopes in macroinvertebrates and fish in two tributaries of the Suyeung River, Korea, before and after heavy monsoon rains to assess changes in community trophic niches. Basal resources (leaf litter and biofilms) exhibited consistent δ13C and δ15N values across seasons, with biofilms showing higher δ13C values. δ15N values increased from forested to agricultural reaches, indicating varied nutrient inputs. Consumer isotope values remained stable over time but varied longitudinally, reflecting reliance on local resources. Trophic niches differed between watershed locations but overlapped seasonally. Despite a decrease in consumer δ13C ranges after heavy rainfalls, variations in their δ15N ranges and the ellipse centroid (SEAc) of isotopic niches between sites resulted in broadly consistent SEAc across locations and seasons. This indicates limited evidence for directional reshaping of food-web properties across channel reaches following monsoon rains. Downstream isotopic shifts suggest substantial agricultural influences on food webs. Overall, our findings highlight that monsoon rains may have minimal effects on the community trophic niches of stream food webs.

Authenticating the Geographical Origin of Jingbai Pear in Northern China by Multiple Stable Isotope and Elemental Analysis.

The Jingbai pear is one of the best pear species in China with high quality and nutrition values which are closely linked to its geographical origin. With the purpose of discriminating the PGI Mentougou Jingbai pear from three other producing regions, the stable isotope ratios and elemental profiles of the pears (n = 52) and the corresponding soils and groundwater were determined using isotope ratio mass spectrometry (IRMS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. The results revealed that δ15N, δ18OJ, and Li were significantly different (p < 0.05) in samples from different regions, which indicated their potential to be used in the geographical origin classification of the Jingbai pear. The nitrogen isotopic values of the pear pulp were positively correlated with the δ15N value and nitrogen content of the corresponding soils, whilst the B, Na, K, Cr, and Cd contents of the pear pulps were positively correlated with their corresponding soils. Orthogonal partial least squares discriminant analysis (OPLS-DA) was performed in combination with analysis of the stable isotopes and elemental profiles, making it possible to distinguish the cultivation regions from each other with a high prediction accuracy (a correct classification rate of 92.3%). The results of this study highlight the potential of stable isotope ratios and elemental profiles to trace the geographical origin of pears at a small spatial scale.

A biogeochemical approach to examining sub-adult diet and the weaning process at Neolithic Tepecik-Çiftlik, Türkiye

Tepecik-Çiftlik, a Neolithic settlement located in the Cappadocia region of Central Anatolia, within the boundaries of Niğde province in modern Türkiye is crucial for helping us to better understand social behaviour and human mobility in the Neolithic, as well as relations between different groups. To investigate dietary habits, subsistence practices, and intra-societal variations stable carbon and nitrogen isotope ratio analysis has been conducted on the Neolithic human and faunal population. Further to this, 21 sub-adults were also sampled for stable carbon and nitrogen isotope ratio analysis to examine sub-adult diet, complementary feeding, and the cessation of breastfeeding as part of the weaning process. This isotopic investigation into the weaning process is complemented by a previously conducted study using Sr/Ca ratios from bone apatite of the same population. The δ13C values of the sampled sub-adults range from −19.8 ‰ to −19.0 ‰ and the δ15N values range from 8.0 ‰ to 15.2 ‰. The isotopic data suggest a very early onset of complementary feeding (ca. 0.2 years of age) and a brief breastfeeding period, relative to other prehistoric populations. The cessation of breastfeeding and this very brief weaning process was likely completed by ca. 1–1.5 years old. This early commencement of complementary feeding was most probably a deliberate social and cultural choice, supported by the presence of bowls and feeding spoons in the graves of very young babies and infants. However, this cultural choice may also have affected the mortality profile of the Neolithic population.

Trophic Structure of Fish Community in Artificial Reef Ecosystem Based on Body Mass Using Stable Isotope

Artificial reefs are widely recognized for their role in improving the ecological environment and creating protected habitats for marine organisms, ultimately enhancing biodiversity within the food web and fisheries resources. This study utilizes stable isotopes to analyze fish samples ranging from 3.4 to 1067 g in body mass, collected within the artificial reef area of Haizhou Bay. The objective is to determine if the δ15N-based fish body mass acts as a driving factor in shaping the food web structure. The results showed a certain level of overlap among all trophic guilds, suggesting that most trophic guilds within this region share similar living environments and feeding habits. The multiple linear regression showed a slight increasing trend between δ15N values and body mass. Furthermore, the predator–prey mass ratio (PPMR) was calculated to be 430:1 based on the δ15N–body mass relationship. This implies that larger reef fish within this artificial reef ecosystem tend to have higher δ15N values compared to smaller fish, indicating a shorter food chain in this ecosystem. In summary, this analysis provides valuable insights into the fish community structure within artificial reef ecosystems. Therefore, it is recommended that future studies focus on further characterizing the fish community structure using body mass information.

Utilization of stable isotopes in teeth to investigate pygmy sperm whales (Kogia breviceps) in the southeastern United States

AbstractThe pygmy sperm whale (Kogia breviceps) is currently the second‐most stranded cetacean in the southeastern United States (SEUS), but information concerning their population structure is severely limited. This study used stable isotope analysis to investigate the isotopic niches and population structure of K. breviceps among three regions in the SEUS determined by the distance of the continental shelf break and a fourth region in the Gulf of Mexico. Teeth from stranded individuals were subsampled via dental drill at four separate areas on each tooth, representing four different age classes: calf, juvenile, subadult, and adult. Stable isotope ratios of carbon and nitrogen were measured in the organic component of 42 teeth, and oxygen isotope ratios were measured in the inorganic (hydroxyapatite) component of 19 teeth. No differences were found in δ13C, δ15N, or δ18O values between regions. However, differences between age classes were significant for adults and subadults for δ13C and adults and juveniles for δ15N. Differences between males and females were also significant for δ15N. Results from this study provide the first carbon and nitrogen isotope values from different age classes of pygmy sperm whales as well as the first reported oxygen isotope values for this species.

Baleen stable isotopes reveal climate-driven behavioural shifts in North Atlantic fin whales

Climate variability impacts the structure and functioning of marine ecosystems and can trigger behavioural responses in organisms. We investigated whether such variability modulates diet and migration in the North Atlantic fin whale (Balaenoptera physalus). To reconstruct the dietary and migratory behaviours over time, we conducted stable isotope analysis of nitrogen (δ15N) and carbon (δ13C) along baleen plates from 29 fin whales sampled off southwestern (SW) Iceland in summer. We estimated a baleen growth rate of 16.1 ± 2.5 cm per year from the stable isotope oscillations observed along the baleens. We also assigned a deposition date for each baleen segment, thus obtaining isotopic sequential time series. We then assessed the potential association of these time series with the main climate patterns of the North Atlantic basin. Baleen δ15N and δ13C values are associated with the North Atlantic Oscillation (NAO) and the Atlantic Multidecadal Oscillation (AMO). During high AMO and low NAO periods, which tend to decrease krill abundance, there is an increase in both the mean and standard deviation of baleen δ15N values, suggesting that fin whales shift to higher trophic resources and expand their dietary niche. Additionally, high AMO periods, which relate to positive temperature anomalies, lead to a decrease in baleen δ13C values, suggesting that fin whales adjust their migratory routes and destinations towards higher latitudes. Significant variation in isotopic niche width between years also reflected these dietary and migratory behavioural shifts. This highlights the plasticity of the North Atlantic fin whale behaviour, a trait likely to strengthen the resilience of the species within the current context of rapid and intense climate variability.

Hydrochemical Characterisation of the Basement Aquifer with a Focus on the Origin of Nitrate in the Highly Urbanised Niamey Region, SW of Niger

This study investigated the basement aquifer beneath the urbanised city of Niamey and the agricultural fields of Kollo, SW of Niger. During the observation period spanning from 2021 to 2023, groundwater and surface water samples were collected for analysis of major ions and the stable isotopes oxygen-18 and deuterium (δ18O and δ2H) of water. To trace the origin of high nitrate concentrations (NO3−) found in several observation and drinking water wells in both areas, δ15N and δ18O isotope values of NO3− were analysed in groundwater and eluted soil samples. The observed hydrochemical patterns mainly reflect the heterogeneity of the weathered fringe of the basement aquifer. Decreasing concentrations of NO3− and δ18O and δ2H values were observed in relation to the distance of the Niger River and increasing thickness of the clay layer on the surface. The wells close to the river in Niamey show a dilution effect during the flood season, and the NO3− concentrations displayed a continuous increasing trend. The δ15N-NO3 and δ18O-NO3 values confirmed that septic tank water is spreading in the region of Niamey and that manure originating from livestock in Kollo is the main source of NO3−. The patterns of δ15N in the soil samples coincide with those of cattle’s manure spread in both areas. The shallow wells show significantly higher values of electric conductivity and NO3− concentrations compared to the deeper wells, which clearly indicates the influence of shallow septic tanks on water quality.