Acid Value Research Articles

Lipidomics reveals the effects of various processing parameters on the lipid composition and activity of roasted coffee oil

Lipids are an important part of the roasting and extraction of coffee, as they impart flavor and aroma to the coffee while it is roasting. Here, coffee oil was extracted from roasted coffee beans, and several ethanol extraction (EE), ultrasound extraction (UE), microwave extraction (ME), and ultrasonic-enzyme extraction (UME) treatments were performed. The oils from the UME group were better in terms of color, acid value, peroxide value, tocopherol content, and other indicators. Glycerolipids, glycerophospholipids, and sphingolipids were evaluated in the three groups via coffee oil analysis via quantitative lipidomics. The sphingolipid and glycerophospholipid metabolic pathways showed considerable lipid enrichment when the identified differential lipids were subjected to metabolic pathway enrichment. Coffee oils subjected to various processing techniques showed substantial changes, as determined by multivariate regression; significantly different lipids were identified as candidate biomarkers. The production of coffee goods can benefit from these new insights into the extraction and application of coffee oil.

The association of perioperative serum uric acid variation with in-hospital adverse outcomes in coronary artery bypass grafting patients.

Previous studies proposed the predictive value of baseline serum uric acid (SUA) in the prognosis of coronary artery bypass grafting (CABG) patients. The association of perioperative SUA variation with in-hospital adverse outcomes in CABG patients is unknown. A total of 2,453 patients were included in the study and were divided into four groups (G1-G4) according to perioperative SUA variation (ΔSUA) (G1, ΔSUA ≤ -90 μmol/L; G2, -90 μmol/L < ΔSUA < 0; G3, 0 ≤ ΔSUA < 30 μmol/L; G4, 30 μmol/L ≤ ΔSUA.) The basic characteristics and incidence of adverse outcomes were compared between the groups in the overall population and the subgroups. Multivariate logistic regression was performed to explore the association between perioperative SUA increases and adverse outcomes, and receiver operating characteristic analysis was used to obtain the cutoff value of SUA increases. The patients had a mean age of 60.9 years and the majority were males (76.7%). In the group with the most significant increase in SUA (G4), incidences of in-hospital all-cause death and fatal arrhythmia were higher than in other groups in the overall population and the subgroups. Multivariate logistic regression showed that an increase in the SUA level of ≥30 µmol/L was significantly associated with in-hospital all-cause death and fatal arrhythmia, independent of the baseline SUA level and renal function. This association was significant in most subgroups for in-hospital fatal arrhythmia and in the ≥60 years, myocardial infarction, and female subgroups for in-hospital all-cause death. The cutoff values of SUA increases in the overall population were 54.5 µmol/L for in-hospital all-cause death and 42.6 µmol/L for in-hospital fatal arrhythmia. The perioperative SUA increase significantly correlated with a higher incidence of in-hospital all-cause death and fatal arrhythmia in CABG patients, independent of the baseline SUA level and renal function. Perioperative SUA variation may provide complementary information in the identification of patients potentially at risk.

Elevated concentrations of soil carbon dioxide with partial root-zone drying enhance drought tolerance and agro-physiological characteristics by regulating the expression of genes related to aquaporin and stress response in cucumber plants

Water scarcity and soil carbon dioxide elevation in arid regions are considered the most serious factors affecting crop growth and productivity. This study aimed to investigate the impacts of elevated CO2 levels (eCO2 at rates of 700 and 1000 ppm) on agro-physiological attributes to induce drought tolerance in cucumbers by activating the expression of genes related to aquaporin and stress response, which improved the yield of cucumber under two levels of irrigation water conditions [75% and 100% crop evapotranspiration (ETc)]. Therefore, two field experiments were conducted in a greenhouse with controlled internal climate conditions, at the Mohamed Naguib sector of the national company for protected agriculture, during the winter seasons of 2021–2022 and 2022–2023. The treatments included eCO2 in soil under normal and partial root zoon drying (PRD, 100% ETc Full irrigations, and 75% ETc). All the applied treatments were organized as a randomized complete block design (RCBD) and each treatment was replicated six times. Untreated plants were designed as control treatment (CO2 concentration was 400 ppm). The results of this study showed that elevating CO2 at 700 and 1000 ppm in soil significantly increased plant growth parameters, photosynthesis measurements, and phytohormones [indole acetic acid (IAA) and gibberellic acid (GA3)], under partial root-zone drying (75% ETc) and full irrigation conditions (100% ETc). Under PRD condition, eCO2 at 700 ppm significantly improved plant height (13.68%), number of shoots (19.88%), Leaf greenness index (SPAD value, 16.60%), root length (24.88%), fresh weight (64.77%) and dry weight (61.25%) of cucumber plant, when compared to untreated plants. The pervious treatment also increased photosynthesis rate, stomatal conductance, and intercellular CO2 concentration by 50.65%, 15.30% and 12.18%; respectively, compared to the control treatment. Similar findings were observed in nutrient concentration, carbohydrate content, Proline, total antioxidants in the leaf, and nutrients. In contrast, eCO2 at 700 ppm in the soil reduced the values of transpiration rate (6.33%) and Abscisic acid (ABA, 34.03%) content in cucumber leaves compared to untreated plants under both water levels. Furthermore, the results revealed that the gene transcript levels of the aquaporin-related genes (CsPIP1-2 and CsTIP4) significantly increased compared with a well-watered condition. The transcript levels of CsPIP improved the contribution rate of cell water transportation (intermediated by aquaporin’s genes) and root or leaf hydraulic conductivity. The quantitative real-time PCR expression results revealed the upregulation of CsAGO1 stress-response genes in plants exposed to 700 ppm CO2. In conclusion, elevating CO2 at 700 ppm in the soil might be a promising technique to enhance the growth and productivity of cucumber plants in addition to alleviating the adverse effects of drought stresses.

Metabolic profile of fatty acids, phenolic compounds, and methylxanthines of cocoa kernels (Theobroma cacao L.) from different cultivars produced in cabruca and full sun farming systems

The demand for high-quality cocoa beans has increased in line with the growing global demand for chocolate. The chemical composition of cocoa beans can vary according to their origin and growing conditions. In this context, this study evaluated the influence of the cultivar type (CCN51 and PS1319) and the cocoa management system (cabruca and full sun) on the chemical composition of unfermented cocoa kernels. The cultivation system influenced the fatty acid composition of cocoa kernels, with higher values of linoleic acid associated with the full sun system, although higher total lipid contents were obtained in the cabruca system. The cultivar influenced the content of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), total unsaturated fatty acids (UFA) and the saturated/unsaturated ratio (S/U). Lower levels of total phenolic compounds, total anthocyanins and antioxidant properties were found in the full sun system, especially in the PS1319 cultivar. Higher levels of epicatechin and catechin were found in the cocoa kernel of the CCN51 cultivar. Theobromine and caffeine were not influenced by the treatments. Neither the PCA of total lipids and fatty acids, nor the PCA of antioxidant properties, phenolic compounds and methylxanthines indicated an isolated clustering between cultivar and cultivation system. The results showed that the factors under study influenced the chemical composition of the unfermented cocoa kernel. Furthermore, they indicated that the migration from traditional systems, such as cabruca, to full sun systems can reduce the total lipids and phenolic compounds content of the cocoa beans. When planning new plantations, the choice of genetic material should also be carefully considered to produce higher-quality cocoa butter and beans with a higher phenolic compound content.

Comparative analysis, nutritional properties, and in- vitro gastrointestinal digestion of the antioxidant potential of roselle leaves using drying techniques

This study aimed to provide new insight into the influence of nutritional properties and in- vitro gastrointestinal digestion on the antioxidant activities of roselle leaves (RL) by applying drying techniques, specifically freeze-drying, oven-drying, sun-drying, and hot air-drying. The results observed that freeze-dried RL had higher nutritional and physical properties compared to other drying techniques. It had the maximum values of protein (6.61 g/100 g), fat (7.74 g/100 g), ash (18.53 g/100 g), crude fiber (9.46 g/100 g), water activity (0.32), pH (2.54), and titratable acidity (0.027%). In addition, all treatments on RL samples were evaluated for their functional characteristics. Furthermore, after in-vitro digestion, the antioxidant potential of total phenolic contents and flavonoid levels exhibited a percentage rise of 2.1% and 12.03% across all treated samples (54.98 ± 0.02 to 57.08 ± 0.05 mg/g and 28.66 ± 0.02 to 40.69 ± 0.03 mg/g), respectively. Interestingly, the total anthocyanin, 2,2-diphenyl-1-picrylhydrazyl, ferric-reducing antioxidant power, and vitamin C in all dried samples levels decreased and ranged from 49.17 ± 02 to 4.91 ± 0.42 mg/g, 84.92 ± 0.21 to 80.81 ± 0.21%, 14.65 ± 0.48 to 1.12 ± 0.21 mg/g, and 8.8 ± 0.06 to 2.24 ± 0.04 mg/L, respectively, after digestion. Consequently, freeze-drying techniques demonstrate advantageous effects on RL antioxidant potential. RL may serve as a valuable component for incorporating into other products to enhance innovative and functional food products.

Temperature dependent sensitivity of the harpacticoid copepod Nitokra spinipes to marine algal toxins

Harmful algal blooms (HABs) – proliferated algae densities, often producing toxins – have increasingly been found in ocean and coastal areas. Recent studies show that rising temperatures contribute to HAB occurrence, but the broader influence of climate change on these outbreaks is less quantified. Of particular concern is the limited research on HAB toxin effects under varying temperatures, especially regarding primary consumers such as copepods, a crucial component of aquatic ecosystems. Therefore, we examined the impact of marine toxins on the harpacticoid copepod Nitokra spinipes, a model organism for marine ecotoxicology, in the context of climate change. We evaluated the toxicity of four purified, commonly occurring algal toxins, at three different temperatures in the laboratory. First, adult females were exposed to a concentration series of toxins at 15, 20, and 25 °C for 48 hours. EC50 values of domoic acid ranged from 8.79 ± 1.93 μg L-1 to 25.97 ± 11.96 μg L-1. Nauplii, aged 48 to 72 hours, were exposed at 18, 20 and 22 °C for the same duration. Less sensitive compared to adults, the EC50 of domoic acid in this case varied from 57.26 ± 6.82 μg L-1 to 97.24 ± 6.45 μg L-1. Both results indicated a temperature-dependent EC50. For the chronic toxicity tests, larval development ratio (LDR), brood size and inter-brood time of domoic acid (DA), yessotoxin (YTX), saxitoxin (STX), and microcystin-LR (MC-LR) were examined at 18, 20 and 22 °C. We observed that with increasing temperatures, LDR increased, whereas brood size significantly decreased as DA, YTX or STX concentrations rose. No interaction between temperature and algal toxins was found but a temperature dependent sensitivity of copepods towards DA, YTX and STX was revealed. Our research provides insights into the effects of long-term exposure to algal toxins on marine copepods and the potential impacts of climate warming.

Low‐Temperature Domestic Deep‐Frying of Soybean‐Cake Tempe in Vegetable Cooking Oils: How Many Times Are Stable to Use?

ABSTRACTTempe has gained global popularity, with local vegetable oils commonly used for frying. This study evaluates the cooking temperature and stability of five vegetable oils (olive [Oo], palm [Po], canola [Cnlo], sunflower [Sfo], and coconut [Cco]) for deep‐frying Tempe, using acid and peroxide values (AV and PV), antiradical activity, and saturated and unsaturated fatty acids. AV, PV, and linolenic acid (LNA) were referenced to international standards for vegetable oil (0.6 mg KOH/g, 10 mEq of oxygen/kg oil, and 2% for AV, PV, LNA respectively). The initial oil temperature was 130°C ± 1°C, with final temperatures between 145.7°C ± 6.8°C at the lowest and 156.8°C ± 13.0°C at the highest, well below existing studies (≥170°C–250°C). Based on AV and PV, Oo, Po, and Cco were stable up to the fourth, fifth, and eighth frying repeat (FR). The PV of Cnlo (10.2 mEq of oxygen/kg oil) and Sfo (15.5 mEq of oxygen/kg oil) exceeded the maximum limit after one use. The Fresh Cnlo LNA (7.35%) was higher than the limit, while the rest of the oils remained lower and stable until the seventh FR. Po exhibited the highest average antiradical activity (85.42% ± 4.63%), followed by Oo (31.01% ± 10.26%), Sfo (27.96% ± 9.67%), Cnlo (21.85% ± 5.71%), and Cco (14.40% ± 3.46%). Cco had the highest saturated fatty acids (SFA), Oo had the highest monounsaturated fatty acids (MUFA), and Sfo had the highest polyunsaturated fatty acids (PUFA). No significant SFA, MUFA, or PUFA changes were observed up to the seventh FR. Trans‐fatty acids C18:1n 9T and C18:2n 6T were undetected in fresh and used oil, indicating a unique character in low‐temperature deep‐frying in domestic settings. This study provides a comprehensive analysis of low‐temperature deep‐frying of Tempe. It suggests that Oo, Po, Cnlo, Sfo, and Cco were stable to deep‐fried Tempe for four, five, zero, one, and eight FR, respectively. Deep‐frying Tempe at lower temperatures and for a shorter duration may enhance its health benefits and help retain its flavor.”

Application of omega 3,6 with Moringa oleifera suplemented vitamin E on rumen fluid characteristics, microbial protein synthesis, and methane gas production in goats on suboptimal lands

Changes in feeding patterns were needed to overcome the problem of methane gas emissions from ruminant livestock and the problem of low productivity at the smallholder livestock level. This solution involves direct action against the gas produced in the stomach of ruminants by providing feed containing active substances to reduce the microbes that produce gas in the stomach. One alternative feed that was often used as a promoter of improving rumen fluid characteristics was the use of omega 3.6 sources together with Moringa leaves enriched with vitamin E. This research aimed to investigate the effect of feeding on methane gas production in goats. The research method used a Randomized Block Design (RAK) with four treatments and five replications in vitro. From the research results, it could be concluded that the use of omega 3.6 together with Moringa leaves enriched with vitamin E was able to reduce methane gas production by 25.62% with a Volatile Fatty Acid (VFA) value of 144.53 mM, pH around 6.99, NH3 11.87 mg/100ml, microbial biomass 20.42 mg/ml, microbial protein 217.58mg/ml, bacterial colony 5.34x109 cells/ml and protozoa population 29x106 cells/ml. This conclusion shows that the use of omega 3.6 with Moringa oleifera supplemented with vitamin E has the potential to consistently improve rumen fluid characteristics and reduce methane gas emissions by using a combination of omega 3.6 sources and Moringa leaves in suboptimal land, as well as vitamin E supplementation.

Optimization of lycopene spray drying encapsulation in basil seed gum: Boosting bioavailability and mayonnaise stability

This study aimed to improve lycopene stability and bioavailability in food products. Lycopene, a potent antioxidant, often has poor stability and undesirable organoleptic properties. Therefore, the impact of basil seed gum (BSG) concentration and spray drying inlet temperature (IT) on the physicochemical, bioaccessibility, and antioxidant properties of encapsulated lycopene emulsion (ENL) was investigated using Central Composite Design (CCD)-Response Surface Methodology (RSM). Optimal encapsulation conditions were IT = 141.96 °C and BSG = 19.507 %. The ENLs had an average particle size of 147.56 nm, a polydispersity index (PI) of 0.263, and a zeta potential of −21.37 mV, indicating good colloidal stability. Antioxidant activity varied slightly during the four weeks of storage (a 9.65 % increase followed by a 13.6 % decrease), but it remained stable overall. Incorporating ENL into mayonnaise significantly reduced the acid value (2.78 mg KOH/g), the anisidine index (12.43), the peroxide value (7.13 meq/kg), and the TBARS index (0.534), and improved color parameters, reducing brightness (79.94) and whiteness (70.64) while masking lycopene's strong yellow and red hues. This study highlights BSG-encapsulated lycopene's potential to improve oxidative stability and sensory properties, offering a natural and effective method to enhance lycopene stability, bioavailability, and sensory acceptance in various food applications.

Real-time monitoring of 2,5-dimethylpyrazine in solid, liquid, and gas phases during processing of fried skipjack tuna steaks using intestinal villus-like Ag nanoparticles@Au nanowires surface-enhanced Raman substrate chips

The production and distribution of 2,5-dimethylpyrazine (2,5-DMP), a key volatile flavor compound, are associated with the frying of skipjack tuna steaks. In this study, an intestinal villus-like chip was fabricated to quantitatively detect 2,5-DMP in multiphase systems based on surface-enhanced Raman spectroscopy (SERS). The chip exhibited excellent SERS performance with an enhancement factor of 1.16 × 108, excellent uniformity and reproducibility, and low detection limits of 6.49 pg/mL, 0.182, and 0.920 ng/mL for 2,5-DMP solid, liquid, and gas models, respectively. The results indicated the 2,5-DMP content with growth rates in the order of frying steam > frying steaks > frying oil, and the 2,5-DMP content in frying steam was linearly correlated (R2 = 0.992) with the trend of the acid value and total polar compounds in frying oil. Therefore, this strategy for achieving the reliable detection of 2,5-DMP can assist in monitoring the frying process and food safety.

Synthesis, Characterization, and Optimization of Antimicrobial Biolubricant Derived from Ocimum lamiifolium and Lactic Acid

Optimal extraction parameters were determined, yielding 12.4% oil at 4.5 hours, with a particle size of 1.375 mm and a solute-to-solvent ratio of 0.28 w/v. The extracted oil exhibited high yield (15% moisture, 0.92 g/mL specific gravity), a volatile lubricant component (3%), a pH of 4.2-5.91, low ash (11.5%), high saponification value (19.7 g KOH/g) and moderate acid number (8.9 mg KOH/g), iodine value of 35.3 g/L, low viscosity (0.1160 Pa.s), and high diffusion capacity (95%). Chemical analysis using UV-Vis spectrophotometry was employed to identify the constituents present in the extract, while FTIR spectra indicated the presence of alcoholic and phenolic components, suggesting interactions with lactic acid. Through GC/MS analysis, a total of 32 phytochemicals were identified, with seven significant ones: Eugenol (Phenol), Isocaryophyllene, Elemicin (benzene), Hexadecanoic acid with methyl ester, Octadecanoic acid with methyl ester, Neophytadiene and Squalene. Evaluation against E. coli, B. subtilis, S. typhimurium, and S. aureus revealed the O. lamiifolium extract and biolubricants most active against S. aureus and E. coli, suggesting potential for superior antibacterial lubricants with this plant extract and lactic acid.

Study of Variation in Physiochemical Properties of a Worm Gearbox Lubricant by Blending Castor Oil in the Base Lubricant

In both industrial &amp; heavy-duty applications, oil analysis can be a very effective strategy for preventing gearbox failure, cutting downtime, and managing maintenance costs. In any application, gearboxes are essential to production, the unplanned downtime and the resulting productivity losses can prove to be extremely expensive. Used oil analysis is a preventative maintenance procedure that can point out and pinpoint mechanical breakdown inside the system before it gets serious or becomes too expensive to fix. A thorough oil examination can identify wear, corrosion, and other changes that could be harmful to the health and durability of the machinery. To reduce machine wear, prevent contamination, or optimize working conditions in challenging environments, lubricants are carefully formulated by mixing additives in the base oil. A lubricant with a high acid content can cause varnish and sludge to accumulate, which can clog oil filters and cause machine parts to corrode. When a lubricant degrades, acidic byproducts are produced because of the base stock and additives' process of decomposition in the presence of heat and air. To assess the presence of acidic constituents in the oil or its acid concentration, Total Acid Number (TAN) oil testing is employed. The present work studies the effects on Total Acid Number (TAN) and Total Base Number (TBN) properties of commercial lubricant (EP 90) when castor oil is mixed in percentage concentration in it. The findings of this research have unveiled that the acidic characteristics of the base lubricant (EP90 commercial gear oil) increases as the percentage of castor oil additive is increased in the base lubricant oil.

Valuation of Ethanol Grade on the Robustness of Acid Number Determination in Fish Oil

The robustness evaluation of the acid number analysis method in fish oil was carried out to see the effect of the type of ethanol used as a solvent in the titration process. The types of ethanol used in this study were pro analysis grade, pharma and food grade, and technical grade. The acid number values (mgKOH/g) in fish oil with various solvents obtained were 2.3955±0.3211 for pro analysis grade; 2.7932±0.2983 for pharma and food grade; 2.7812±0.3362 for technical grade 1; 2.7031±0.3405 for technical grade 2. The acid number for all types of ethanol has a value that is in accordance with the requirements of SNI 8467: 2018 where the acid number value must be less than 3 mgKOH/g. However, if the measurement uncertainty value is included in the calculation, only the pro analysis grade ethanol solvent has an acid number value that is in accordance with the requirements of SNI. The results of the ANOVA test also showed that there was a significant difference in the variation of ethanol types for determining acid numbers because the calculated F value (13.9004) was greater than the F table (3.0984). Therefore, the solvent that must be used in the method of determining acid numbers in fish oil is pro analysis grade ethanol.

Unique fatty acid composition of coriander seed biodiesel

Biodiesel is a renewable and environmentally friendly alternative fuel that can be used in diesel engines without modification. In this study, biodiesel production from coriander seed oil obtained through a two-stage transesterification process using the cold pressing method was carried out in the Konya region. First, the oil's acid value was reduced through acid-catalyzed pretreatment to form coriander seed oil methyl esters, and then biodiesel was produced using standard transesterification with methanol and sodium methoxide catalyst. Coriander (Coriandrum sativum) contains an unusual fatty acid not previously reported as a major component in biodiesel fuels: petroselinic acid (6Z-octadecenoic acid; 68.5% by weight). Coriandrum sativum seed oil methyl ester was evaluated to have properties suitable for diesel engines, complying with EN 14214 and ASTM D6751 standards, with a unique fatty acid composition and excellent oxidative stability.

ANALISIS SIFAT FISIKA DAN SIFAT KIMIA GEL BIODIESEL DARI MINYAK BIJI PALEM PUTRI (Adonidia merrillii)

Biodiesel is an alkyl ester of long-chain fatty acids, the most common of which are methyl esters or ethyl esters. The manufacturing process involves esterification and transesterification. Traditional sources of biodiesel raw materials such as palm oil, soybeans, and jatropha have been widely studied. The princess palm, widely known as an ornamental plant, produces fruit containing seeds with quite high oil potential. This research aims to produce biodiesel and determine the physical and chemical properties from putri palm seeds. This research is experimental research with a quantitative descriptive approach. Putri palm seed oil is extracted using the soxhletation method with n-hexane solvent. The oil was then esterified using methanol with a catalyst of 1% sulfuric acid with a mole ratio of oil and methanol of 1:8 at a temperature of 60°C for 3 hours. The esterified oil is then transesterified using methanol and KOH with a methanol to oil ratio of 1:20 with KOH of 0.8% for 2 hours at a temperature of 60°C. This research has succeeded in making biodiesel from putri palm seeds. The biodiesel produced is in the form of a gel referred to as biodiesel gel with a yellowish brown color with a density of 1,236 gr/ml and a pour point of 36-38°C. The FFA/ALB value is 0.587%, the acid number is 1.177 mg KOH/g, the IOD number is 34.514 (g-I2/100 g), the saponification number is 63.19 mg KOH/g, the heating value is 48.088 MJ/Kg, and the cetane number 38,620. The IOD number, saponification number, and heating value meet the SNI standards for liquid biodiesel in general, while the cetane number and ALB value are close to SNI standards.

The Role of Serum Level of Col2a1 and Hyaluoronic Acid in Diagnosis of Knee Osteoarthritis

Osteoarthritis (OA) is a complex condition in which affected tissues experience changes in metabolism, structure, biochemistry, and function. This condition arises when chondrocytes fail to balance the synthesis and degradation of extracellular components like proteoglycans and collagens, leading to inflammation of the synovial membrane and joint capsule. Aim of the study: This study was designed to evaluate the serum levels of Type II Collagen alpha 1 (coll2a1) and Hyaluronic Acid (HA) in diagnosis of early stage of knee osteoarthritis in Iraqi patients Subjects and Methods: This case- control study was carried out at Department of Biochemistry, College of Medicine, University of Baghdad and at Rheumatology Department , Baghdad Hospital / Medical City, during the period from September 2022 to March 2023. It included ninety volunteer males and females subdivided into subgroups: Group 1consisted of 50 patients who have had early stages of knee osteoarthritis (KOA) and group 2 involved 40 apparently healthy subjects who served as control group. The diagnosis of KOA was made by a consultant rheumatologist using American College of Rheumatology (ACR) criteria. Moreover, patients were sub- classified according to their BMI (normal weight, overweight and obese) and KOA severity (grad1 and grad 2). Investigations included serum measurements of Human serum coll2a1( type II collagen alpha I) and Human serum HA (Hyaluronic Acid) in all patients and controls by ELISA technique. Results: The mean (±SD) values of Coll2a1, HA, of KOA patients were significantly higher than those of controls (for all, p &lt;0.01). There was significant positive correlation between. The mean value of serum hyaluronic acid was significantly increased in grad 2 KOA when compared with grad 1 KOA patients (p &lt; 0.004). The results found that fibulin-3 has the highest receiver operator characteristic (ROC) and area under curve (AUC=89.2% ) at cutoff (7.50 ng/ml) with sensitivity 80.0 and specificity 80.0 in diagnosis of KOA. Also, serum Coll2a1 has high diagnostic utility (AUC=88.6%) at cutoff (286.00 pg/ml) with sensitivity 82.0 and specificity 80.0 . Conclusion: Assessing non-invasive serum levels of coll2a1 (with a cutoff of 286.00 pg/ml) offers a new biochemical marker for diagnosing the early stages of knee osteoarthritis (KOA) potentially before changes become visible on X-rays. Additionally, measuring serum hyaluronic acid levels could serve as an indicator of disease severity, helping to differentiate between various grades of KOA.

Starch lysophospholipids contents affect storage quality of paddy rice

Starch Lysophospholipids (LPLs) have a significant effect on rice grain quality, but how starch LPLs affect paddy rice storage quality is barely understood. The dynamic changes in storage quality indicators of a japonica rice (XS110) and its low LPLs (XS110-J1) and high LPLs (XS110-J2) mutants were investigated. The fatty acid values (FAV) of J1 were significantly higher than those of XS110 and J2 after one year of storage under 24 and 37 °C. Although FAV rose gradually during storage time, it increased relatively more at 37 °C than 24 °C. The malondialdehyde (MDA) content increased significantly in the early stage and decreased gradually during later storage. The MDA content of rice stored at 24 °C was significantly higher than that at 37 °C. The catalase (CAT) activity exhibited a decreasing trend during storage and was relatively higher at 24 °C than 37 °C. The CAT of J2 was significantly higher than those of XS110 and J1. Total lysophospholipid content was relatively stable although some individual LPLs changed significantly during storage. The fatty acid component content in the milled rice decreased during storage, and the content at 37 °C was lower than that at 24 °C. In conclusion, paddy rice with low starch LPLs content is more susceptible to deterioration during storage, suggesting that high starch LPLs can better maintain rice quality and prolong storage time during storage of paddy rice.

Glycyrrhetinic acid reduces lung inflammation caused by pneumococcal infection by reducing the toxicity of pneumolysin

ObjectiveIn this study, to provide new methods for the treatment of Streptococcus pneumoniae infection, we aimed to describe the anti-inflammatory and antibacterial value of glycyrrhetinic acid on the basis of its inhibitory effect on bacterial growth (without killing the bacteria) and its reduction of the toxicity of S. pneumoniae. MethodsA mouse model was established via intranasal administration of Streptococcus pneumoniae D39, and glycyrrhetinic acid was subcutaneously injected for treatment. The wet‒dry ratio, bacterial flora content and inflammatory factor levels in the mouse lungs were determined. Cell experiments were used to evaluate glycyrrhetinic acid-mediated inhibition of PLY hemolysis and A549 cell death, and WB was used to measure glycyrrhetinic acid-mediated inhibition of PLY oligomerization. ResultsGlycyrrhetinic acid reduced the levels of inflammatory factors, the dry‒wet ratio, the abundance of S. pneumoniae in the lungs of infected mice, pneumolysin-mediated A549 cell death, erythrocyte hemolysis and PLY oligoplasia. ConclusionGlycyrrhetinic acid can reduce the virulence of S. pneumoniae by preventing the oligomerization of PLY.

Effect of Red Guava (Psidium guajava L.) Juice Concentration on Product Quality and Antioxidant Activity of Red Guava Synbiotic Yoghurt

Red guava juice has very strong antioxidant activity and has the potential to be processed into synbiotic functional beverage products that have health benefits. This study examined the effect of red guava juice concentration on product quality and antioxidant activity of red guava synbiotic yoghurt. Product quality tested included microbiological quality (total LAB) and chemical quality (pH value and Total Tritable Acid (TTA )value). The concentrations of red guava juice used were 0%, 10%, 20%, 30%, and 40%. Fermentation was carried out for 6 hours at 37⁰C by Lactic Acid Bacteria (LAB) from powdered mixed culture. Then organoleptic testing using the hedonic test, total LAB testing using the Total Plate Count (TPC) method, pH value testing using a pH meter, TTA value testing using the acid-base titration method, and antioxidant activity testing using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. Test data were analyzed using SPSS software to determine the effect of red guava juice concentration on test results. The concentration of red guava juice in red guava synbiotic yoghurt affects the pH value, TTA value, and IC50 value as the result of antioxidant activity testing. Red guava juice concentration did not affect total LAB testing. Organoleptic assessment of red guava symbiotic yoghurt is in the range of like to like very much. The best formulation was red guava synbiotic yoghurt with 30% red guava juice concentration, which had strong antioxidant activity with an IC50 value of 59.637 ppm, pH value of 3.94, TTA value of 1.98%, and total LAB as much as 1.81x10⁸ colonies/g, so it has met the quality requirements of yoghurt according to Indonesian National Standard SNI 2981: 2009.

Biodiesel synthesis from low cost biomass wastes and its cost assessment inducing process optimization

This study employed low-cost biomass wastes for the synthesis of biodiesel that is cost-effective and environmentally friendly. The major raw material (oil) was obtained by steam distillation (SD) from Croton heliotropiifolius Kunth leaf (CHKL) and was characterized for its aptness for biodiesel production. Dwarft green coconut husk ash (DGCHA) was used as a bio-adsorbent for acid value reduction of Croton heliotropiifolius Kunth leaves oil (CHKLO). A novel, highly potassium-based catalyst was derived from Karpuravalli banana peels (KBP), calcined, and characterized using TGA, ZETA, FTIR, SEM-EDX, XRF-FS, and BET analysis. Biodiesel was synthesized using a microwave-assisted method, characterized, and compared with the recommended standard. The catalytic strength of the calcined Karpuravalli banana peel powder (CKBPP) was tested using a reusability test, and the cost evaluation of production was estimated. Results showed that the CHKL was rich in oil (43% wt./wt.), and the oil is highly acidic (5.23 mg KOH/g oil). At high particle size, the dwarf green coconut husk ash (DGCHA) bagasse reduced the acid value to a minimum (1.4 mg KOH/g oil) at 3 days. The developed novel catalyst from CKBPP indicated high potassium-calcium contents for base transesterification. Process optimization indicated that the predicted response data of 95.285% (wt./wt.) at T1 = 90 min, T2 = 60 oC, T3 = 4.5% (wt.), and T4 = 9 (vol./vol.) was validated in triplicate, and the average data value of 95.10% (wt./wt.) was established. Dataset on the quality of biodiesel showed that the produced biodiesel properties were in line with recommended standards. Economic appraisal data showed that the cost of producing 20 L of CHKLOB (biodiesel) was $4.73 at 1,500 to $1. The study concluded that the production of biodiesel from waste can be cost-effective and environmentally friendly if wastes are harness.Graphical