White Blood Cell Values Research Articles

Growth performance, haemato-biochemical indices of broiler chicken fed Aristolochia indica as a phytogenic feed additive

This experiment was carried out to examine the growth performance and some heamato-biochemical indices of broiler chicken fed Aristolochia indica (ALM) as a phytogenic feed additive. A total of 500 – 1-day old (Vencobb 450) with initial body weight of 48.0 ± 0.02 g were randomly divided to 5 groups with 5 replicates for each group. Basal diet (a corn-soya meal based diet) was sufficient in all nutrients (NRC, 1994). Birds in group 1 was fed basal diet without A. indica leaf meal, those in group 2, 3, 4 and 5 were fed basal diet with supplemented with A. indica leaf meal at 100 g, 200 g, 300 g and 400 g per 100 kg-1 respectively. Fresh clean water and feed were offered ad libitum. Results on phyto-constituents of Aristolochia indica leaf meal showed that it contained flavonoids (996.05 mg/100 g-1), tannins (609.7 mg/100 g-1), phenols (1341.8 mg/100 g-1), steroids (206.17 mg/100 g-1), alkaloids (151.8 mg/100g) and saponins (91.21 mg/100g). Average daily weight gain, average daily feed intake and feed conversion ratio were higher in group 4 and 5, intermediate in group 3 and lower in group 1 and 2 (P < 0.05). Mortality of 2.20 % was recorded in group 1 while none was recorded in the other treatment (P < 0.05). Pack cell volume, red blood cell, haemoglobin, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, white blood cell, lymphocytes and monocyte values were significantly (P < 0.05) different among the groups except for basophil values (P > 0.05). Total protein, albumin/globulin ratio, urea, creatinine, glucose and cholesterol were influenced (P < 0.05) by the treatment except for alkaline phosphatase, alanine transaminase and aspartate transaminase values (P > 0.05). In conclusion, ALM can be fed up to 400 g in the diet of broilers without compromising their performance and health status.

Inflammation in aortic surgery: postoperative evolution of biomarkers according pathologies and segments of the aorta

ObjectivesAortic pathologies often present with elevated inflammatory biomarkers due to the nature of the disease. Open aortic surgery causes significant trauma to the body due to often mandatory ischemic periods, long cardiopulmonary bypass times and polytransfusion. We aim to determine postoperative trends on inflammation biomarkers for different aortic pathologies and type of surgery in different segments of the aorta.MethodsRetrospective review of prospectively collected data of 193 consecutive patients who underwent aortic surgery in our centre between 2017 and 2021, grouped according to the type of aortic intervention: (1) Type A aortic dissection (AD) repair with ascending aorta/hemiarch replacement, (2) Aortic root replacement (ARR), (3) Aortic arch + Frozen elephant trunk (FET), (4) Descending thoracic aorta (DTA)/Thoraco-Abdominal aortic repair (TAA). Primary outcomes were daily values of white blood cells (WBC) and C-Reactive Protein (CRP) during the first 15 postoperative days.ResultsAll groups had a similar inflammatory peak in the first 2–4 days (WBC 12-15 × 109 c/L). AD and FET groups show similar trends with WBC and CRP peaks on days 2 and 10. The ARR group didn’t experience the 2nd peak as most patients were already discharged. DTA/TAA patients experienced a more prolonged inflammatory response, reaching a plateau by day 5–10. AD group shows the highest WBC levels and the DTA/TAAA group the highest CRP levels. CRP levels remain elevated (100–200 mg/L) in all groups after 15 postoperative days.ConclusionsInflammatory biomarkers show different postoperative trends depending on the clinical presentation and complexity of the aortic procedure performed. Further understanding of the inflammatory response to different aortic pathologies and surgical procedures will permit reduction on the liberal use of antibiotics that this cohort of patients are usually exposed to. An earlier version of the data included in this manuscript was presented as Oral in the UK Society of Cardiothoracic Surgery Annual meeting in 2021

The Effect of Crystal Arthropathy on the Diagnostic Criteria of Native Septic Arthritis.

Distinguishing between septic arthritis and crystal arthropathy flares can be challenging. The purpose of this study was to determine how the presence of synovial crystals affects the diagnostic criteria of septic arthritis. A retrospective review identified patients undergoing joint aspirations to rule out native septic arthritis. Differences between septic arthritis presenting with and without synovial crystals were analyzed. A receiver-operating characteristic curve was plotted for laboratory markers to determine the area under the curve, or diagnostic accuracy, for septic arthritis and to evaluate thresholds that maximized sensitivity and specificity. There were 302 joint aspirations in 267 patients. Septic arthritis was diagnosed in 17.9% (54/302). Patients with synovial crystals were less likely to have septic arthritis (4.2% [5/119] vs. 26.8% [49/183], P < 0.0001). Septic arthritis in patients with no synovial crystals was associated with fever and a higher synovial white blood cell (WBC) count, synovial polymorphonuclear cell percentage (PMN%), serum WBC, and C-reactive protein (CRP) ( P < 0.05). Septic arthritis in patients with synovial crystals was only associated with inability to bear weight and a higher synovial WBC and CRP ( P < 0.05). Synovial PMN% was considered nondiagnostic of septic arthritis (area under the curve 0.56) in patients with crystals while synovial WBC and CRP had acceptable (0.76) and excellent (0.83) diagnostic utility, respectively. The WBC and CRP value thresholds that maximized sensitivity and specificity for septic arthritis were greater in patients with crystals (21,600 vs. 17,954 cells/μL and 125 vs. 69 mg/L, respectively). The presence of synovial crystals reduced the likelihood of septic arthritis and altered the laboratory diagnostic criteria. PMN% was nondiagnostic in the setting of synovial crystals.

The impact of wet cupping on haematological and inflammatory parameters in a sample of Jordanian team players

Background/objectiveThe practice of complementary and alternative medicine has significantly gained acceptance worldwide, such as Al-Hijama, also known as cupping therapy. Despite the growing popularity of therapeutic cupping among athletes, little is known about the impact of cupping therapy on sports fields. The current study was designed to explore the effect of wet cupping therapy on the haematological and inflammatory parameters in Jordanian national team players. MethodsThe procedure was carried out at a specialized centre for cupping in Amman on the morning of the 19th Rajab. The data were obtained from 14 healthy male participants aged between 21 and 22 years. The haematological and inflammatory parameters were assessed by comparing venous blood components before and after four weeks of wet cupping. ResultsComplete blood count (CBC) analysis of venous blood samples four weeks after wet cupping showed a significant increase in the values of total white blood cells (WBCs), neutrophils, lymphocytes, red blood cells (RBCs), haematocrit, and haemoglobin as compared with venous blood samples before cupping. Blood film examination of venous blood samples post-cupping revealed normocytic normochromic RBCs; WBCs and platelets were unremarkable. Analysis of inflammatory markers post cupping showed a significant decrease in the monocyte/lymphocyte ratio (MLR) and platelet/lymphocyte ratio (PLR) but no differences in neutrophil/lymphocyte ratio (NLR). ConclusionThe findings of this study suggest that wet cupping has an indispensable influence on haematological and immunological parameters in athletes, where it reinforces cellular immunity, generates younger blood cells, and reduces inflammation markers. It is probable, therefore, that cupping improves sports performance and achievement. The evidence from this research adds to a growing body of literature on cupping therapy in sports.

Intersecting pathways: evaluating inflammatory markers and metabolism in chronic spontaneous urticaria with a multi-marker approach.

Chronic spontaneous urticaria (CSU) is an inflammatory skin disease with intricate mechanisms. This study comprehensively assessed markers from diverse metabolic pathways, including novel inflammatory indicators, to evaluate their potential for diagnosing and monitoring CSU. In the study involving 90 CSU patients and 90 healthy controls, the levels of albumin, high-density lipoprotein (HDL), fibrinogen, uric acid, D-dimer, C-reactive protein (CRP), and white blood cells (WBC) values were analyzed. The D-dimer/albumin ratio (DAR), fibrinogen/albumin ratio (FAR), and uric acid/HDL ratio (UHR), considered novel inflammatory markers, were calculated. The Urticaria Activity Score 7 (UAS7) was also calculated. Pearson chi-squared test, Mann-Whitney U test, Spearman correlation coefficient, and univariate logistic regression analysis were employed for data analysis. In the patient group, significant elevations were observed in DAR, FAR, fibrinogen, CRP, D-dimer, and UHR values. Additionally, albumin, HDL, and uric acid values exhibited significant decreases. HDL and albumin provided the most accurate results in the univariate logistic regression analysis. CRP had less accuracy, FAR exhibited greater accuracy than fibrinogen, and DAR demonstrated higher accuracy than D-dimer. There was no statistically significant correlation between the UAS7 and parameters. The considerable correlation of CRP with other parameters, except D-dimer, was also remarkable. Indicators from diverse metabolic pathways, including albumin, HDL, uric acid, fibrinogen, D-dimer, and CRP, can be valuable in assessing CSU. In particular, FAR and DAR are emerging as potential markers to consider in the assessment of CSU.

Parasitological and serological detection of Trypanosoma evansi on Bali cattle at the Pesanggaran slaughterhouse, Denpasar, Indonesia using hematological profile

Abstract. Apsari IAP, Suratma NA, Swacita IBN, Soma IG, Sari TK, Putra IPC, Sudipa PH. 2024. Parasitological and serological detection of Trypanosoma evansi on Bali cattle at the Pesanggaran slaughterhouse, Denpasar, Indonesia using hematological profile. Biodiversitas 25: 1057-1062. Bali cattle (Bos sondaicus Blyth, 1842 syn. Bos javanicus domesticus Wilckens, 1905) are vulnerable to blood protozoan infections like Trypanosoma evansi. Indonesia's warm and moist environment provides ideal conditions for blood-sucking flies such as Stomoxys calcitrans, Haematobia irritans, and Hipobosca sp. to thrive and spread. These flies act as carriers of T. evansi, which can then infect Bali cattle. This study aims to detect the presence of T. evansi in Bali cattle. Blood samples were collected from 275 Bali cattle at the Pesanggaran slaughterhouse, Denpasar City, Bali, Indonesia. The samples were examined using the Giemsa-Staining Blood Smear (GSBS) method and hematology profile examination using Hematology Analyzer RT-7600 for Vet. Serum samples were also collected for serologic examination using the Card Agglutination Test for Trypanosomiasis (CATT) method. The hematological profile data obtained were analyzed descriptively, and the Mann-Whitney U test was used to determine the significance of hematological values between female and male cattle seropositive to T. evansi. The results showed that the T. evansi parasite was not detected in the blood smear examination. However, the serological detection with CATT revealed that 6.54% (18/275) of Bali cattle were positive for T. evansi. The hematological profiles of T. evansi seropositive cattle remained within the normal range, except for neutrophil percentage. White Blood Cell (WBC), Hemoglobin (Hb) levels, and hematocrit (PCV) values of male cattle were significantly (P?0.05) lower than those of females. Therefore, based on serological examination, it can be concluded that the protozoa T. evansi is present in 6.54% of Bali cattle at the Pesanggaran slaughterhouse.

Immuno-Haematological Indices Stabilizing Attributes of Co-Enzyme Q10 Supplementation in Thioacetamide Poisoned Wistar Rats

Coenzyme Q10 (CoQ10 or Co_Q10) as a known endogenous compound has been linked to some therapeutic functions, thus, the need for further exploration in more dysfunctional biological scenarios. This present study, thus, evaluated the stabilizing attributes of Co-enzyme Q10 supplementation in thioacetamide (TAA) altered immuno-haematological system in Wistar rats. One hundred and twenty (120) male Wistar rats weighing 250 and 280g were used for the study and were randomly separated into four sub-groups of 30 rats per treatment interval of week 3, 6, 9 and 12. In the individual weeks, the rats were further grouped into six different groups of five rats each. The groups included Group 1-control and received 1ml of normal saline (intraperitoneally―ip), Group 2 received only 200 mg/kg TAA (ip) twice weekly, Group 3 received 5mg/kg Co_Q10, daily per oral (po), Group 4 received 10mg/kg Co_Q10 daily (po), Group 5 received 200 mg/kg TAA (ip) twice weekly and 5mg/kg Co_Q10 (po) daily and Group 6 received 200 mg/kg TAA (ip) twice weekly and 10mg/kg Co_Q10 (po) daily. After weeks 3, 6, 9 and 12 of treatments, blood samples were collected and instantly transferred into well labeled blood sample bottles with anticoagulant. After appropriate laboratory and statistical analyses, the quantitative results were obtained. The result showed that following TAA toxicity, the levels of white blood cells (WBC) were significantly (P&lt;0.05) reduced; meanwhile, those of macrophage and tissue necrotic factor alpha (TNFα) were remarkably (P&lt;0.05) increased. However, upon the supplementations with Co-Q10 in the respective weeks (3, 6, 9 and 12) of treatment, all the aforementioned deranged values of WBC, macrophages and TNFα were significantly (P&lt;0.05) stabilized/normalized (i.e. brought to similar range of the normal untreated rats). Therefore, Co-Q10 supplementation may be of beneficial therapeutic value in particular haematological dysfunctional scenarios.

The Relationship Between Endoscopic Findings and Laboratory Results in Inflammatory Bowel Diseases

Objective: The aim of this study was to determine the relationship between routine laboratory indicators (including hemoglobin, white blood cells, platelets, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP)) and the extent of endoscopic involvement in individuals with inflammatory bowel disease (IBD). Additionally, it sought to examine the applicability of these less invasive parameters for monitoring patients in the follow-up. Material and Method: The medical records of patients who were diagnosed with Ulcerative Colitis (UC) and Crohn's Disease (CD) between 2009 and 2015 were retrospectively examined. Endoscopic findings and hemoglobin, white blood cell, platelet, ESR, and CRP values at the time of colonoscopy were analyzed. An exploratory multinomial regression model was created to examine the association of laboratory parameters and endoscopic involvement localization. Results: In UC, a significant decrease in hemoglobin levels was present in cases with extensive colitis/pancolitis compared to distal type colitis (p=0.02), while no significant difference was found between left-sided colitis and distal type colitis. Elevated ESR values were notably found in left-sided colitis (p=0.007) and extensive colitis/pancolitis (p=0.043) compared to distal type colitis. CRP levels were significantly higher in cases with extensive colitis/pancolitis (p=0.015). No relationship was identified between laboratory parameters and the endoscopis location of involvement in CD. Conclusion: Although hemoglobin value, ESR and CRP levels are helpful in determining the location of involvement in UC, their effects have not been observed in CD. In addition to these basic laboratory values, other parameters should also be taken into consideration in the evaluation of patients.

Enterovirus and Paraechovirus Meningitis in Neonates: Which Is the Difference?

Enterovirus (EV) and parechovirus (HPeV) are common viruses in the neonatal period, with similar seasonality and symptomatology. They also are the main causes of aseptic meningitis in newborns and children under 1 year of age. We compared the clinical signs, laboratory data, brain, and neurodevelopmental outcome of 10 infants with HPeV and 8 with EV meningitis. In patients with EV meningitis, serum C-reactive protein (CRP) values were significantly higher than those of patients with HPeV infection. Procalcitonin values were low in both groups. White blood cell (WBC) and lymphocyte values were significantly higher in EV patients. None of the infants had a brain lesion on cerebral ultrasound neither negative neurological outcome. Based solely on symptoms, it is not possible to distinguish HPeV from EV infection. C-reactive protein, WBC, and lymphocyte values might allow the physician to assume EV infection. The gold standard test for diagnosis remains real-time polymerase chain reaction on cerebral spinal fluid.

Values of White Blood Cell Count and C-Reactive Protein in Diagnosis of Acute Appendicitis

Background and objectives. Acute appendicitis is one of the most common surgical emergencies. Accurate diagnosis of acute appendicitis is based on careful history, physical examination, and laboratory investigation. The purpose of this study is to investigate the value of white blood cells (WBC) and C-reactive protein (CRP) in the diagnosis of acute appendicitis. Methods. A retrospective analysis of 350 files of patients who underwent appendectomy was conducted. Selected files were those patients who were admitted to ALmasara clinic Tripoli Libya between Aug 2011 to July 2023, ages of patients ranged from 18 to 80 years. Statistical analysis was performed using SPSS version 26 software. A statistical procedure was implemented to compare the hematologic parameters in two groups (Males and Females) to determine if there was any significant difference between them. Means of WBC, Neutrophil, Lymphocyte and CRP were compared between two groups by an independent one-way analysis of variance (ANOVA). Results. This study consisted of 350 participants who were diagnosed with appendicitis. The results showed that about (69.4%) of appendicitis patients exhibited a high white blood cell count (leukocytosis). Slightly over half (52.6%) of the participants had an elevated neutrophil percentage (neutrophilia). Approximately (64.3%) of the appendicitis patients had decreased lymphocyte percentage (lymphopenia). In relation to CRP values, the study showed that most participants (76.5%) had positive CRP results. Conclusion. Individuals with appendicitis have higher levels of leukocytes, neutrophils, and C-reactive protein, and decreased lymphocyte counts. Elevated WBC and CRP values together provide a better specificity and positive predictive value than individual markers.

Study on the correlation between controlling nutritional status score and clinical biochemical indicators in patients with colorectal cancer

PurposeThe controlling nutritional status (CONUT) score is an important tool for predicting the prognosis of colorectal cancer (CRC); however, its effectiveness is relatively insufficient. This study aimed to screen for more effective clinical indicators as supplements to the CONUT scoring system and improve the predictive value of CRC prognosis. Patients and methodsBetween 2014 and 2020, the clinical information of all CRC patients in our unit was retrospectively collected, and the CONUT scores were calculated based on the levels of serum albumin (ALB), lymphocytes (LC), and total cholesterol. The included patients were divided into the following three groups: normal nutrition (0–1), mild malnutrition (2–4), and moderate-to-severe malnutrition (5–12). The correlations between the CONUT score and baseline characteristics and clinical indicators were evaluated. ResultsThis study ultimately included 5014 CRC patients. The nutritional status of patients with colon cancer (CC) was worse than that of rectal cancer (RC). The nutritional status was worse in men than in women. The older the patient, the poorer the nutritional status, and the poorer the nutritional status, the longer the hospital stay. In addition, poor nutritional status in patients is indicated by higher values of neutrophils (NE), monocytes (MC), eosinophils (EOS), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), carcinoembryogenic antigen (CEA), and lower values of white blood cells (WBC), basophils (BAS), haemoglobin (HB), total protein (TP), triglycerides (TG), low density lipoprotein (LDL), aspartate transaminase (AST), and blood urea nitrogen (BUN), which was statistically significant (P < 0.05). Indicators that significantly correlated with the CONUT score reflected the immune nutritional status, including WBC (odds ratio [OR] = 0.036, P < 0.001), NE (OR = 30.815, P < 0.001), MC (OR = 41.388, P < 0.001), EOS (OR = 27.577, P < 0.001), BAS (OR = 0.006, P = 0.046), and LDL (OR = 0.319, P < 0.001). ConclusionAdditional variables such as WBC, NE, MC, EOS, BAS, and LDL may be used as supplementary indicators in the CONUT scoring system to more effectively predict the clinical prognosis of CRC patients.

Serum peripheral markers for inflammation in adolescents with anorexia nervosa

Background There is a limited number of studies comparing the levels of inflammation in adolescent patients with anorexia nervosa (AN) and healthy controls based on complete blood count and platelet parameters. Methods This study is a retrospective cross-sectional analysis of 53 drug-naive patients with AN and 53 healthy controls. Results Significant differences were observed for WBC (white blood cell), neutrophil, MCV (mean corpuscular volume), MCH (mean corpuscular haemoglobin) and neutrophils/lymphocytes ratio (NLR) between the study groups. Patients with AN had lower WBC, neutrophiles and NLR values. But there was no difference between the groups in terms of inflammation-related platelet parameters. A strong positive correlation between BMI (body mass index) and PLT (platelet), PCT (plateletcrit) was determined in the patient group (r = 0.454, p = 0.001; r = 0.386, p = 0.007). Inflammation-related parameters may increase as BMI increases with nutrition and weight restoration. Conclusions The present study provides further evidence for level of inflammation in these patients does not increase during the acute period, unlike other mental diseases.

Testing the detoxification power of black cumin oil (Nigella sativa) over cypermethrin insecticide effects in rainbow trout (Oncorhynchus mykiss) at multiple scales

This study investigated the curative effect of black cumin oil (Nigella sativa, NS), which is a phytotherapeutic agent against to cypermethrin (CYP), which is known to have adverse effects on rainbow trout (Oncorhynchus mykiss)’s behavioral changes, oxidative stress-mediated neurotoxicity, hematotoxicity and hepatotoxicity parameters. At the end of the trial period; (i) evaluation of critical swimming speed (Ucrit) (ii) hematology indices [white blood cell (WBC), red blood cell (RBC), hemoglobin (Hgb), hematocrit (Hct), mean cell volume (MCV), mean cell hemoglobin) (MCH), mean cell hemoglobin concentration (MCHC)] (iii) Elucidation of the mechanism of functional damage in brain tissue of O. mykiss by neurological parameter [acetylcholinesterase (AChE)] (iv) Evaluation of oxidative damage in oxidative stress-mediated neurotoxicity and hepatotoxicity in liver, gill and brain tissue of O. mykiss with antioxidant enzymes [(Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), Glutathione (GSH)] and [(detection by means of malondialdehyde (MDA)] (v) Obtaining applicable data in the toxicological field using a multi-biomarker approach to investigate the modulation of NS administration via target markers in the physiological pathway of O. mykiss were aimed. As a result of CYP application, it was determined that the Ucrit value of O. mykiss decreased significantly. It was determined that the changes in the values of RBC, Hgb and Hct, which are among the hematology parameters examined in the blood tissue, were statistically significant (p < 0.05). It was determined that WBC value was inhibited by CYP application and NS tried to make a positive contribution to WBC. It was determined that the AChE activity of O. mykiss in the brain tissue had a statistically significant inhibition in the CYP-treated group (p < 0.05). SOD, CAT, GPx, enzyme activities were found to be inhibited by CYP application and were statistically significant (p < 0.05). Acute toxicity of CYP was determined by antioxidant enzyme biomarkers in gill tissue. In the results obtained; While inhibitions were determined in SOD, CAT, GPx activities compared to the control group, an induction occurred in MDA value. NS administration was noted to be an important modulator of the SOD-CAT system against CYP exposure at both concentrations. Thus, it can be said that it indirectly functions as an effective antioxidant through the NS receptor protein and structurally stimulates the synthesis and activity of antioxidative enzymes under oxidative stress.

Prognostic value of preoperative white blood cell to hemoglobin ratio and fibrinogen to albumin ratio in patients with colorectal cancer.

The prognostic value of preoperative white blood cell to hemoglobin ratio (WHR) and fibrinogen to albumin ratio (FAR) in colorectal cancer (CRC) is unknown. The purpose of this study was to analyze the correlation between preoperative WHR and FAR and the prognosis of CRC patients. The retrospective study analyzed the medical records of 207 patients with colorectal cancer who were admitted to Linyi People's Hospital between June 1, 2017 and June 1, 2021. The receiver operator curve was used to determine the cutoff value of 4.604 for WHR and 0.086 for FAR, and the patients were divided into high and low groups for comparative analysis of clinical data. Cox proportional hazards regression models were used to assess independent risk factors for disease-free survival (DFS) and overall survival (OS) in univariate and multifactorial analyses. Kaplan-Meier methods were used for survival analysis and logrank tests were used to assess survival differences. Multifactorial Cox analysis showed that tumor pathological stage (HR = 6.224, 95% CI:3.063-12.647, P < .001), and WHR (HR = 3.681, 95% CI:1.768-7.401, P < .001) were the independent risk factors for DFS in CRC patients. Tumor pathological stage (HR = 4.080, 95% CI:1.992-8.360, P < .001), and WHR (HR = 3.397, 95% CI:1.662-6.940, P = .001) were independent risk factors for OS. High levels of WHR and high levels of FAR were associated with lower DFS (P < .001) and OS (P < .001).CRC patients with both higher WHR and FAR had significantly lower DFS (P < .001) and OS (P < .001). DFS and OS may be shorter in CRC patients with high WHR and high FAR, perhaps associated with poor prognosis in CRC patients, and WHR and FAR may be potential CRC prognostic markers.

Predictive value of white blood cell to hemoglobin ratio for 30-day mortality in patients with severe intracerebral hemorrhage.

To explore the predictive value of white blood cell to hemoglobin ratio (WHR) for 30-day mortality in patients with intracerebral hemorrhage (ICH). In this cohort study, 2,848 patients with ICH were identified in the Medical Information Mart for Intensive Care (MIMIC)-III and MIMIC-IV. Least absolute shrinkage and selection operator (LASSO) regression screened covariates of 30-day mortality of ICH patients. COX regression analysis was used to study the association of different levels of WHR, white blood cell (WBC), and hemoglobin (Hb) with 30-day mortality. The median follow-up time was 30 (20.28, 30.00) days. In total, 2,068 participants survived at the end of the follow-up. WHR was negatively correlated with the Glasgow Coma Score (GCS) (spearman correlation coefficient = -0.143, p < 0.001), and positively associated with the Sepsis-related Organ Failure Assessment (SOFA) score (spearman correlation coefficient = 0.156, p < 0.001), quick SOFA (qSOFA) score (spearman correlation coefficient = 0.156, p < 0.001), and Simplified Acute Physiology Score II (SAPS-II) (spearman correlation coefficient = 0.213, p < 0.001). After adjusting for confounders, WHR >0.833 (HR = 1.64, 95%CI: 1.39-1.92) and WBC >10.9 K/uL (HR = 1.49, 95%CI: 1.28-1.73) were associated with increased risk of 30-day mortality of patients with ICH. The area under the curve (AUC) value of the prediction model based on WHR and other predictors was 0.78 (95%CI: 0.77-0.79), which was higher than SAPSII (AUC = 0.75, 95%CI: 0.74-0.76), SOFA score (AUC = 0.69, 95%CI: 0.68-0.70) and GCS (AUC = 0.59, 95%CI: 0.57-0.60). The level of WHR was associated with 30-day mortality in patients with severe ICH, and the WHR-based prediction model might provide a tool to quickly predict 30-day mortality in patients with ICH.

Is there still an indication for the Birmingham Hip Resurfacing in femoral head osteonecrosis?

Osteonecrosis of the femoral head (ONFH) is a progressive hip disease. Hip resurfacing arthroplasty (HRA) is a preferred surgical procedure among hip arthroplasty performed in young patients. The aim of this study is to show the long-term clinical and radiological results of HRA procedures performed for patients suffering from ONFH. Forty-five patients who underwent hip resurfacing with the diagnosis of femoral head osteonecrosis were included in the study. The Harris Hip Score (HHS) was used for clinical scoring of the patients. The blood chromium, cobalt, white blood cell (WBC) count, and CRP levels of patients were checked. Ultrasonography (USG) was performed for all patients at mid-term control checked for pseudo-tumours. For the radiological evaluation, acetabular inclination, stem shaft angle, prothesis-to-neck ratio, osteolysis zones, and heterotopic ossification were used. The mean age of the patients was 46.6 ± 9.3years, and the mean follow-up period was 11.83 ± 2.9years. The mean HHS was 90.3 ± 12.8 for final follow-up. The mean WBC value was 8.2 109/L, mean CRP value was 6.3mg/L, mean chromium value was 4.9µg/L, and mean cobalt value was 1.8µg/L. Inclination changing 0.2°(p = 0.788), stem shaft angle changing 0.7°(p = 0.424), and neck-to-prosthesis ratio changing 0.01°(p = 0.075). No pseudo-tumours were detected in any patients in USG examination. HRA provides long-term implant survival and excellent clinical outcomes for end-stage ONFH patients with low complication rates.

The predictive value of hemogram parameters for early preterm delivery in pregnant women undergoing cervical cerclage.

This study aims to investigate the predictive value of hemogram parameters in early preterm delivery (32 gestational weeks and below) among pregnant women who have undergone cervical cerclage, based on cervical changes determined before the cerclage procedure. Between 2010 and 2020, a total of 161 patients underwent cervical cerclage. The participants were divided into three groups. Group 1 (n=92) consisted of pregnant women who underwent prophylactic cerclage. Group 2 (n=31) included those with cervical shortening (<5 mm) and/or dilation (≤3 cm). Group 3 (n=38) comprised pregnant women with cervical dilation >3 cm. Each group was further divided based on delivery weeks, with a cutoff at 32 weeks. Demographic parameters and laboratory parameters were assessed. In Group 1, all hemogram parameters showed no significant differences between deliveries below and above 32 weeks. In Group 2, the neutrophil-to-lymphocyte ratio value before cerclage was higher in the early preterm delivery group (p=0.002), with a cutoff value of 4.75 in receiver operating characteristic analysis. In Group 3, the white blood cell value before cerclage was higher in the early preterm delivery group (p=0.005), with a cutoff value of 13.05×103/μL in receiver operating characteristic analysis. The use of hemogram parameters to predict early preterm delivery in pregnant women undergoing prophylactic cerclage is not appropriate. However, neutrophil-to-lymphocyte ratio value can predict early preterm delivery when cervical dilation is 3 cm or less and/or cervical shortening is 5 mm or less. When cervical dilation exceeds 3 cm, the white blood cell value is more appropriate for predicting early preterm delivery.

Predictive value of white blood cells count to mean platelets volume ratio in patients with ST segment elevation myocardial infarction undergoing primary percutaneous coronary intervention

Predictive value of white blood cells count to mean platelets volume ratio in patients with ST segment elevation myocardial infarction undergoing primary percutaneous coronary intervention

Prevalence of Anemia in Libyan Patients with Celiac Disease Before and After a Gluten-free Diet

Abstract BACKGROUND: This study aimed to estimate the incidence of anemia among celiac patients before and after their intake of a gluten-free diet (GFD). METHODS: A retrospective cross-sectional study was conducted in different departments of internal medicine, gastroenterology, and pediatrics at Tripoli University Hospital, Tripoli, Libya. The study data were retrospectively extracted from the clinical files of 100 patients who were diagnosed with celiac disease (CD). Hematological results of complete blood count for each patient before and after induction of GFD for 1 year were collected from the medical records. The data were analyzed using IBM SPSS version 26 software. RESULTS: Out of 100 CD patients, 53 patients were female and 47 patients were male. The analysis showed that the values of red blood cell, hemoglobin, hematocrit, mean cell hemoglobin (MCH), and MCH concentration were significantly higher after treatment compared to before one (P &lt; 0.05). However, the independent t-test analysis did not show a significant difference in the mean values of white blood cell and red cell distribution width before and after treatment (P &gt; 0.05). In addition, the mean value of platelets was lower after treatment with a P = 0.047. CONCLUSION: Anemia was a common finding in the studied cases. Although the GFD improved CD symptoms, anemia persisted in a number of CD patients, even after a year of GFD treatment.

Evaluation of hematologic and coagulation system disorders in adolescents with polycystic ovary syndrome

Aim: Polycystic ovary syndrome (PCOS) is characterized by hyperandrogenism, ovulatory dysfunction, insulin resistance, and obesity, affects 8-26% of adolescent girls. The effects of polycystic ovarian syndrome on the hematologic and coagulation systems have received little attention. We aimed to investigate the hematologic and coagulation system disorders in PCOS. Materials and Methods: The study included 40 adolescents with PCOS and 41 healthy controls. Hemostatic and coagulation system parameters evaluated in both groups and were compared with metabolic parameters. Results: Adolescent’s with PCOS had a significantly higher insulin, HOMA-IR levels, and Hematocrit (Hct) levels than the control group, but no significant difference was found in D-dimer, fibrinogen, protein C, protein S, Antitrombine III levels. A positive correlation was found between insulin and HOMA-IR values and hemoglobin (Hgb), Hct, white blood cell (WBC) levels (r=0.32 p=0.003; r=0.33 p=0.002; r=0.36 p=0.001, respectively); also a positive correlation was found between high-density lipoprotein cholesterol (HDL) and WBC values (r=0.26 p=0.01). A positive correlation was found between follicle stimulating hormone (FSH) and Protein C and Protein S (r=0.41 p=0.008; r=0.31 p=0.04); total testosterone and fibrinogen levels (r=0, 32 p=0.04); Dehydroepiandrostenedione sulfate (DHEAS) and protein S levels (r=0.31 p=0.04). Conclusion: There was no significant difference in the hematologic and coagulation parameters between PCOS and healthy controls. Hypercoagulated state in PCOS is associated with obesity, BMI, inflammation, and insulin resistance, not specific to the disease.