Quercus Lobata Research Articles

Stabilizing selection for within‐season flowering phenology confirms pollen limitation in a wind‐pollinated tree

Summary1. We examined the fitness consequences of pollen limitation in the valley oak (Quercus lobata Née, Fagaceae), a wind‐pollinated, predominantly outcrossing tree endemic to California, by examining the relationship between within‐year phenology and acorn production over an 8 year period.2. We predicted that pollen limitation should result in trees flowering relatively early and late in the season, when fewer trees are in flower, to experience reduced reproductive success compared to trees flowering during the middle of the season.3. Despite considerable variation in acorn production from year to year, analyses confirmed this prediction and indicated that there was significant overall stabilizing selection on budburst date consistent with the pollen limitation hypothesis.4. Analyses including herbivory and pre‐budburst temperatures at individual trees revealed significant correlations with acorn production, but we still found significant stabilizing selection on budburst date even when considering these variables.5. Synthesis. Pollen limitation, as indicated by stabilizing selection on within‐year flowering phenology, significantly affects acorn production by individual trees in Q. lobata and has the potential to influence other key life‐history features including masting behaviour and spatial synchrony.

The New Kid (review)

Reviewed by: The New Kid Deborah Stevenson Jukes, Mavis. The New Kid. Knopf, 2011. [288p]. Library ed. ISBN 978-0-375-95879-3 $17.99 Trade ed. ISBN 978-0-375-85879-6 $14.99 E-book ed. ISBN 978-0-375-89631-6 $9.99 Ad Gr. 3–5. A move from southern to northern California means that eight-year-old Carson is now the New Kid in his class at Valley Oak School. That’s a daunting position, especially when obstreperous classmate Weston Walker seems bent on making Carson into his sidekick, if not actual partner, in crime, complicating Carson’s quest to find a friend with whom to celebrate his upcoming birthday. As the days go by, however, Carson begins to settle in, making friends with his classmates and the class rat and discovering that Weston’s not the troublemaker he appears. Veteran author Jukes has a fluid and readable style, and Carson, with his love of animals and his attachment to his beloved stuffed animal, Moose, is an endearing protagonist. The story is somewhat unshaped, though, so it’s left to the reader to identify the main points among the other quotidian events; aside from Weston, there’s little characterization of the secondary players, who tend to blend together save for the odd gimmick, and Weston’s misjudged nature requires a number of contrived misunderstandings. The easygoing prose and the humor-touched world of the middle-grades classroom will likely still satisfy fans of Claudia Mills and Johanna Hurwitz. Copyright © 2012 The Board of Trustees of the University of Illinois

Consumer control of oak demography in a Mediterranean-climate savanna

Tree recruitment is rare and oak populations are declining in many Mediterranean-climate oak savannas. Factors affecting acorn production, seedling establishment and initial seedling growth have been much studied in short-term experiments. However, fecundity and early survival rates have not been placed in a demographic framework that also considers sapling survival and growth and adult tree mortality. We use matrix models and life table response experiments to analyze long-term experimental and observation data on California valley oak (Quercus lobata Nee´) near its southern range limit in Santa Barbara County to answer three questions: (1) How sensitive is oak population growth rate to variation in acorn production and initial seedling establishment vs. sapling survivorship and growth? (2) How do mammalian consumers—specifically, cattle, deer and rodents—impact valley oak seedling establishment and sapling survival and growth? (3) Can vertebrate consumers account for the observed population decline of valley oak in savannas in its southern range? We find that population growth is far more sensitive to consumer- mediated variation in sapling survivorship and growth than to variation in fecundity or seedling establishment and that consumers exert strong influence on the demography of the species. Deterministic, finite population growth rate (k )i s,1 for unprotected plants and for plants that are protected from cattle but still exposed to mule deer and rodents. Population growth rate increases to 1.03 with protection from both cattle and deer, mainly because plants are able to quickly reach the tree layer when they are protected from ungulate browsing. Population growth rate jumps to 1.15 for plants protected from both ungulates and rodents as a result of increased survivorship and height growth of established individuals and because of increased seedling establishment during the first year. Our experimental findings are consistent with observed patterns in natural populations in Santa Barbara County, where tree recruitment is rare in both grazed and non-grazed savannas but more common in areas such as roadsides that are refuges from cattle and deer.

Interspecific and Intraspecific Pollination Patterns of Valley Oak,Quercus lobata, in a Mixed Stand in Coastal Central California

Pollination patterns within Quercus lobata and interspecific hybridization between Q. lobata and Quercus douglasii were studied in a coastal central California mixed woodland. We first identified hybrids by means of microsatellite genotyping and assignment tests. Hybrids were rare, both among adults (4 of 190, 2.1%) and among acorns collected from Q. lobata trees (6 of 392, 1.5%). These low rates of hybridization at both early and late life history stages suggest that fertility barriers, rather than natural selection against hybrids, limit hybridization between these two species. However, hybrid adults, although rare, may facilitate gene flow between the two species. Acorns collected from a hybrid tree had both Q. lobata pollen donors (11 of 30, 37%) and Q. douglasii or hybrid pollen donors (19 of 30, 63%). After removing hybrid acorns from the analysis, we used paternity assignment to track pollination patterns within Q. lobata. Of 108 acorns, only 32 (30%) were assigned to candidate pollen donors within 200 m of the maternal tree, indicating that the majority of effective pollen travels more than 200 m. Individual trees had acorn crops with many different sires and an average effective number of pollen donors (Nep) of 219 per tree. Indirect methods using correlated paternity estimated mean pollination distances of ∼100 m and mean Nep of 5.2 per tree, values much lower than those derived directly from paternity assignments.

Surface chemistry variations among a series of laboratory-produced biochars

While the idea that adding pyrogenic carbon (referred to as ‘biochar’ when used as a soil amendment) will enhance soil fertility and carbon sequestration has gained widespread attention, understanding of its chemical and physical characteristics and the methods most appropriate to determine them have lagged behind. This type of information is needed to optimize the properties of biochar for specific purposes such as nutrient retention, pH amelioration or contaminant remediation. A number of surface properties of a range of biochar types were examined to better understand how these properties were related to biochar production conditions, as well as to each other. Among biochars made from oak ( Quercus lobata), pine ( Pinus taeda) and grass ( Tripsacum floridanum) at 250 °C in air and 400 and 650 °C under N 2, micropore surface area (measured by CO 2 sorptometry) increased with production temperature as volatile matter (VM) decreased, indicating that VM was released from pore-infillings. The CEC, determined using K + exchange, was about 10 cmol c kg −1 for 400 and 650 °C chars and did not show any pH dependency, whereas 250 °C biochar CECs were pH-dependant and rose to as much as 70 cmol c kg −1 at pH 7. Measurements of surface charge on biochar particles indicated a zeta potential of − 9 to − 4 mV at neutral pH and an iso-electric point of pH 2–3. However, a colloidal or dissolved biochar component was 4–5 times more electronegative. Total acid functional group concentration ranged 4.4–8.1 mmol g −1 (measured by Boehm titration), decreased with production temperature, and was directly related to VM content. Together, these findings suggest that the VM component of biochar carries its acidity, negative charge, and thus, complexation ability. However, not all acid functional groups exchanged cations as the number of cation exchanging sites (CEC) was about 10 times less than the number of acid functional groups present on biochar surfaces and varied with biomass type. These findings suggest that lower temperature biochars will be better used to increase soil CEC while high temperature biochars will raise soil pH. Although no anion exchange capacity was measured in the biochars, they may sorb phosphate and nitrate by divalent cation bridging.

Fitness consequences of seed size in the valley oak Quercus lobata Née (Fagaceae)

We examined the functional relationship between seed size and seedling performance in the valley oak (Quercus lobata Nee) by means of a 13-year common garden experiment. Acorns were collected from five localities throughout the range of valley oak in autumn 1997, weighed and measured, and planted at Sedgwick Reserve, Santa Barbara County, California, USA. In the short term, larger acorns produced larger seedlings that had lower survival than seedlings from smaller acorns. In the longer term, large seeds correlated positively with both seedling size and survival, with path analyses indicating that the latter effect was primarily indirect via initial seedling size. The longer-term relative growth rate was only weakly related to seed size, being a combination of a slight positive direct influence of seed size on relative growth rate and a comparable negative indirect effect via larger initial seedling size. These results generally matched the predictions of the “seedling size effect hypothesis” (larger seeds yield larger seedlings with greater competitive abilities), the only one of the three hypotheses we examined that predicts an inverse relationship between seed size and initial survival and a positive relationship between seed size and longer-term relative growth rate. The factors influencing the relationships between seed size and seedling performance are complex and may involve both direct effects of seed size and indirect effects mediated through initial seedling size. Although the seedling size effect was the most important in our study, other factors may be important under different environmental conditions and/or at different growth stages.

Shifting Baselines in a California Oak Savanna: Nineteenth Century Data to Inform Restoration Scenarios

For centuries humans have reduced and transformed Mediterranean‐climate oak woodland and savanna ecosystems, making it difficult to establish credible baselines for ecosystem structure and composition that can guide ecological restoration efforts. We combined historical data sources, with particular attention to mid‐1800s General Land Office witness tree records and maps and twentieth century air photos, to reconstruct 150 years of decline in extent and stand density of Valley oak (Quercus lobata Neé) woodlands and savannas in the Santa Clara Valley of central coastal California. Nineteenth century Valley oak woodlands here were far more extensive and densely stocked than early twentieth century air photos would suggest, although reconstructed basal areas (7.5 m2/ha) and densities (48.9 trees/ha) were not outside the modern range reported for this ecosystem type. Tree densities and size distribution varied across the landscape in relation to soil and topography, and trees in open savannas were systematically larger than those in denser woodlands. For the largest woodland stand, we estimated a 99% decline in population from the mid‐1800s to the 1930s. Although most of the study area is now intensely developed, Valley oaks could be reintroduced in urban and residential areas as well as in surrounding rangelands at densities comparable to the native oak woodlands and savannas, thereby restoring aspects of ecologically and culturally significant ecosystems, including wildlife habitat and genetic connectivity within the landscape.

Effects of Prescribed Fires on Young Valley Oak Trees at a Research Restoration Site in the Central Valley of California

Woodland restoration sites planted with Quercus lobata (valley oak) often have serious invasions of nonnative annual grasses and thistles. Although prescribed fire can effectively control these exotics, restoration managers may be reluctant to use fire if it causes substantial mortality of recently planted saplings. We studied the effects of prescribed fires on the survival and subsequent growth of 5‐ and 6‐year‐old valley oak saplings at a research field near Davis, California. One set of blocks was burned in summer 2003 at a time that would control yellow star thistle, a second set of blocks was burned in spring 2004 at a time that would control annual grasses, and a third set was left unburned. Very few oaks died as a result of either fire (3–4%). Although a large proportion was top‐killed (66–72%), virtually all these were coppiced and most saplings over 300 cm tall escaped top‐kill. Tree height, fire temperature, and understory biomass were all predictive of the severity of sapling response to fire. Although the mean sapling height was initially reduced by the fires, the growth rates of burned saplings significantly exceeded the growth rates of unburned control trees for 2 years following the fires. By 2–3 years after the fires, the mean height of spring‐ and summer‐burned saplings was similar to that of the unburned control saplings. The presence of valley oak saplings does not appear to preclude the use of a single prescribed burn to control understory invasives, particularly if saplings are over 300 cm tall.

Foraging patterns of acorn woodpeckers (Melanerpes formicivorus) on valley oak (Quercus lobata Née) in two California oak savanna-woodlands

Landscape characteristics and social behavior can affect the foraging patterns of seed-dependent animals. We examine the movement of acorns from valley oak (Quercus lobata) trees to granaries maintained by acorn woodpeckers (Melanerpes formicivorus) in two California oak savanna-woodlands differing in the distribution of Q. lobata within each site. In 2004, we sampled Q. lobata acorns from 16 granaries at Sedgwick Reserve in Santa Barbara County and 18 granaries at Hastings Reserve in Monterey County. Sedgwick has lower site-wide density of Q. lobata than Hastings as well as different frequencies of other Quercus species common to both sites. We found acorn woodpeckers foraged from fewer Q. lobata seed source trees (K g = 4.1 ± 0.5) at Sedgwick than at Hastings (K g = 7.6 ± 0.6) and from fewer effective seed sources (N em* = 2.00 and 5.78, respectively). The differences between sites are due to a greater number of incidental seed sources used per granary at Hastings than at Sedgwick. We also found very low levels of seed source sharing between adjacent granaries, indicating that territoriality is strong at both sites and that each social group forages on its own subset of trees. We discovered an interesting spatial pattern in the location of granaries. At Sedgwick, acorn woodpeckers situated their granaries within areas of higher-than-average tree density, while at Hastings, they placed them within areas of lower-than-average tree density, with the outcome that granaries at the two sites were located in areas of similar valley oak density. Our results illustrate that landscape characteristics might influence the number of trees visited by acorn woodpeckers and the locations of territories, while woodpecker social behavior, such as territoriality, shapes which trees are visited and whether they are shared with other social groups.Electronic supplementary materialThe online version of this article (doi:10.1007/s00442-010-1828-5) contains supplementary material, which is available to authorized users.

Trade-offs between vegetative growth and acorn production in Quercus lobata during a mast year: the relevance of crop size and hierarchical level within the canopy.

The concept of trade-offs between reproduction and other fitness traits is a fundamental principle of life history theory. For many plant species, the cost of sexual reproduction affects vegetative growth in years of high seed production through the allocation of resources to reproduction at different hierarchical levels of canopy organization. We have examined these tradeoffs at the shoot and branch level in an endemic California oak, Quercus lobata, during a mast year. To determine whether acorn production caused a reduction in vegetative growth, we studied trees that were high and low acorn producers, respectively. We observed that in both low and high acorn producers, shoots without acorns located adjacent to reproductive shoots showed reduced vegetative growth but that reduced branch-level growth on acorn-bearing branches occurred only in low acorn producers. The availability of local resources, measured as previous year growth, was the main factor determining acorn biomass. These findings show that the costs of reproduction varied among hierarchical levels, suggesting some degree of physiological autonomy of shoots in terms of acorn production. Costs also differed among trees with different acorn crops, suggesting that trees with large acorn crops had more available resources to allocate for growth and acorn production and to compensate for immediate local costs of seed production. These findings provide new insight into the proximate mechanisms for mast-seeding as a reproductive strategy.

Gene movement and genetic association with regional climate gradients in California valley oak (Quercus lobataNée) in the face of climate change

Rapid climate change jeopardizes tree populations by shifting current climate zones. To avoid extinction, tree populations must tolerate, adapt, or migrate. Here we investigate geographic patterns of genetic variation in valley oak, Quercus lobata Née, to assess how underlying genetic structure of populations might influence this species' ability to survive climate change. First, to understand how genetic lineages shape spatial genetic patterns, we examine historical patterns of colonization. Second, we examine the correlation between multivariate nuclear genetic variation and climatic variation. Third, to illustrate how geographic genetic variation could interact with regional patterns of 21st Century climate change, we produce region-specific bioclimatic distributions of valley oak using Maximum Entropy (MAXENT) models based on downscaled historical (1971-2000) and future (2070-2100) climate grids. Future climatologies are based on a moderate-high (A2) carbon emission scenario and two different global climate models. Chloroplast markers indicate historical range-wide connectivity via colonization, especially in the north. Multivariate nuclear genotypes show a strong association with climate variation that provides opportunity for local adaptation to the conditions within their climatic envelope. Comparison of regional current and projected patterns of climate suitability indicates that valley oaks grow in distinctly different climate conditions in different parts of their range. Our models predict widely different regional outcomes from local displacement of a few kilometres to hundreds of kilometres. We conclude that the relative importance of migration, adaptation, and tolerance are likely to vary widely for populations among regions, and that late 21st Century conditions could lead to regional extinctions.

Phylogenetic and trait similarity to a native species predict herbivory on non-native oaks.

Introduced plants tend to experience less herbivory than natives, although herbivore loads vary widely. Herbivores may switch hosts onto an introduced plant for at least two reasons. They may recognize the novel plant as a potential host based on similarity of the plant's traits to the traits of one of its native hosts, a similarity that may or may not exhibit phylogenetic signal. Alternatively, herbivores may feed optimally, assessing which introduced plants provide the best nutrition irrespective of similarity to native species. Here, we created a phylogeny of 57 oak (Quercus) taxa, which were grown outside of their ranges in a common botanical garden that contained one abundant native oak (Quercus lobata). We used the phylogeny to estimate the phylogenetic conservatism of herbivory by two feeding guilds of insects (leaf chewers and leaf miners) and 11 plant traits expected to affect herbivore performance. We found high phylogenetic signal in chewing damage but not mining damage and all traits except for leaf maturation time. Introduced oaks that are more closely related to the native oak received more chewing and mining damage than distantly related oaks, and introduced oaks that had greater overall similarity in leaf traits also received higher chewing damage but not mining damage. These results demonstrate that interactions between introduced plants and their herbivores are driven independently by traits that track plant phylogeny and leaf traits that likely affect herbivore performance.

Relative contribution of contemporary pollen and seed dispersal to the effective parental size of seedling population of California valley oak (Quercus lobata, Née)

For plant populations, gene movement through pollen and seed dispersal governs the size of local genetic neighbourhoods and shapes the opportunities for natural selection and genetic drift. A critical question is how together these two processes influence the evolutionary dynamics of local populations. To assess the respective contributions of pollen and seed flow, we propose a novel indirect assessment of the separate male and female gametic contributions to total effective parental size (N(e)), based on parental correlations estimated via kinship coefficients, that can be applied to data sets that include unambiguous genotypes for male and female gametic contributions. Using the endemic Californian valley oak (Quercus lobata) as our study species, we apply this method to a set of microsatellite genotypes for two distinct ecological sets of naturally recruiting seedlings with acorns attached. We found that the effective numbers of contributing male parents (N(ep)) exceed effective numbers of female parents (N(em)) for seedlings established beneath adult trees (N(ep) = 8.1 and N(em) = 1.1), as well as for seedlings established away from adult trees (N(ep) = 15.4 and N(em) = 2.7), illustrating that seed dispersal enhances pollen dispersal and increases the effective number of seed sources in open seedling patches. The resulting effective parental size of seedling populations translates into smaller effective numbers of parents for undispersed vs. dispersed seedlings (N(e) = 3.6 and N(e) = 6.7, respectively). This study introduces a novel statistic method and provides important new evidence that, on a short-term temporal scale, seed dispersal shapes the local neighbourhood size of new recruits.

Support for the microenvironment hypothesis for adaptive value of gall induction in the California gall wasp, Andricus quercuscalifornicus

AbstractThree major hypotheses have been advanced for the adaptive nature of plant galls: nutrition, enemy‐avoidance, and microenvironment. Of these, the microenvironment hypothesis has been frequently invoked, but rarely tested directly. We tested this hypothesis in a population of Andricus quercuscalifornicus (Bassett) (Hymenoptera: Cynipidae) wasps inducing galls on Quercus lobata Née (Fagaceae) trees in Northern California, USA. Relative humidity and temperature data gathered from both immature and mature galls in the field indicated that A. quercuscalifornicus larvae modify their microenvironments significantly by raising and stabilizing relative humidity levels inside galls to near saturation. In addition, excised larvae maintained under experimental conditions survived significantly longer under levels of high relative humidity. These data support the hypothesis that through gall induction, A. quercuscalifornicus manipulates its environment adaptively.

Short distance pollen movement in a wind-pollinated tree, Quercus lobata (Fagaceae)

Short distance pollen dispersal shapes the local genetic structure of plant populations and determines the opportunity for genetic drift and local selection. In this paper we focus on short distance dispersal (SDD) of pollen in a low-density stand of a savannah oak, Quercus lobata Née. Specifically, we are interested in the proportional contributions of pollen donors, the pollen dispersal kernel that describes local matings, the extent to which wind influences mating success, and the extent to which pollen sources vary within the large canopy of these trees. Using maximum likelihood paternity analysis, we assigned sires for 474 outcrossed progeny of five seed trees, representing 120 of 160 potential mating pairs within a 250m radius of each focal tree (ca. 20ha plots). We first established that the effective number of pollen donors for progeny with sires within the plot was about 10 individuals, with average weighted pollination distances of 114.1m. We estimated 18.5% pollen immigration into the 20ha plots. We next established that the SDD portion of the dispersal kernel is best described by the exponential power, inverse power, and Weibull functions, all that capture high local dispersal with steep decay. Two of these models suggest that long distance dispersal is abundant, represented by a fat tail, while the Weibull indicates depauperate long distance dispersal, represented by a thin tail. The addition of a directional component corresponding to the predominant wind axis had no meaningful impact on these models. Finally, we established that different parts of an individual tree canopy of Q. lobata sample from the same homogeneous pollen pool showing no bias towards pollen sources near that part of the canopy. Overall findings suggest low-density Q. lobata populations show steep decay of SDD. Policies and ordinances governing the amount of allowable tree removal of savannah oak populations should recommend the preservation of local clusters of adults, as well as some connectivity among clusters.

No Trade‐Off between Seed Size and Number in the Valley OakQuercus lobata

We examined the relationship between acorn mass and number in valley oaks (Quercus lobata) over 4 years in central coastal California. Despite considerable variation in acorn size among both trees and years, trees produced acorns of the same size relative to other trees in different years. Across years, the relationship between acorn mass and acorn crop size was generally positive, even after controlling for environmental conditions and differences in individual tree size and quality. Life-history trade-offs in valley oaks are primarily between current and future reproduction and indirectly between concurrent growth and reproduction, not between seed size and number, and are probably related to this species' mast-seeding behavior. Phenotypic trade-offs in long-lived plants such as oaks exhibit complex patterns of life-history covariation and deserve greater attention, both theoretically and empirically.

Regional analysis of riparian bird species response to vegetation and local habitat features

Abstract We investigated relationships between riparian bird abundance and local vegetation characteristics and habitat features across the Sacramento/San Joaquin Valley, California. Number of detections was analyzed for each of 21 species from point count surveys over a 4-year period at 22 sites from three regions (Sacramento River, Cosumnes River, and San Joaquin River) in relation to 16 measures of habitat and vegetation composition within 50 m of 184 survey points. Tree variables, including tree height and trunk diameter, were often important, as was specific composition of tree species, especially Fremont cottonwood (Populus fremontii) and valley oak (Quercus lobata). Effects of mugwort (Artemisia douglasiana) and blackberry (Rubus spp.) were generally positive. The median partial R2 due to vegetation/habitat characteristics was 16% after controlling for regional differences in abundance per species. Comparisons of model results at the local versus regional scale revealed spatial variation in bird ab...

Prescribed Fire Effects on Deciduous Oak Woodland Stand Structure, Northern Diablo Range, California

Despite the increasing use of fire in managing oak woodlands, little information exists on quantitative changes to stand structure from prescribed burning. Fire damage and recovery in a mixed deciduous oak woodland were recorded after a prescribed fire on the northern Diablo Range, Santa Clara County, California. Blue oak (Quercus douglasii Hook. & Arn.), valley oak (Q. lobata Nee), and black oak (Q. kelloggii Newb.) trees were monitored for 4 yr to determine the effects of a late spring burn on stand structural characteristics. Fire-caused mortality was low; 4 yr after the low intensity ground fire only four oaks died (1.9%). There were significant differences in mean percent tree crown scorch and mean trunk char height between plots that burned under different fire intensities, but not between tree size classes. Although overall tree damage was low, crown resprouts developed on 80% of the trees and were found as shortly as 2 wk after the fire. Recovery was vigorous; both valley oaks and blue oaks produced crown resprouts on trees with 100% crown scorch. Classification tree analysis identified aspect (mostly southern exposures) and tree size related to the presence of crown resprouting. Crown damage was also an important factor; trees with greater than 40% of their crown scorched resprouted. Fire-induced trunk scars occurred on a small number of trees (9.1%) but was disproportionately higher for black oak compared to blue and valley oak. Stand structural characteristics (species composition, tree density, basal area, and crown closure) were not substantially altered by the event but rather maintained. Prescribed fire might be a viable tool in reducing fuels and maintaining oak woodlands; however, further investigations that include relationships of regeneration with repeated fire are needed.

The relative importance of factors affecting age-specific seedling survival of two co-occurring oak species in southern California

As has been reported in other oak habitats throughout the Northern Hemisphere, natural recruitment rates of young oaks in California are very low for some species and in some regions. The majority of experimental studies that contribute to our understanding of this oak recruitment pattern in California have been relatively short-term, conducted on a small-scale. Thus, while we have valuable information about the array of factors that are able to limit seedling establishment, we know much less about their relative importance, and how they vary across years, sites, or age classes. To investigate the impacts of factors limiting seedling and sapling establishment of valley oak ( Quercus lobata) and coast live oak ( Quercus agrifolia) across a landscape and over time we replicated experiments in four different years in Santa Barbara County, California. Experimental factors manipulated included cattle grazing, and access by deer and small mammals. Plots were distributed across three sites with a cumulative area of 200-ha, and seedling survival was recorded for a minimum of 5 years. We used classification and regression tree analysis (CART) to examine the relative importance of factors influencing survival of planted oaks at different life stages. The relative importance of limiting factors varied among age classes for both species. For initial seedling emergence and survival to 6 months planting year was the most important factor and rodent access was the second most important factor for both Q. lobata and Q. agrifolia. For survival of seedlings through their 1st year rodents, planting year, and site were major limiting factors, though their relative importance varied between the two species. For survival from 1.5 to 5 years, Q. lobata was strongly affected by rodents and site, while Q. agrifolia was mainly affected by site, deer browsing (which reduced survival), and indirect effects of winter–spring cattle grazing (which improved survival). Contrary to our expectations, based on observed patterns of natural recruitment, Q. agrifolia had equivalent or lower survivorship than Q. lobata in all seedling age classes. Overall, in addition to other factors, there were strong year and site effects controlling oak seedling emergence and survival and the relative importance of limiting factors depended on seedling age. Our results suggest that large-scale, long-term experiments encompassing multiple sites will improve our understanding of controls on recruitment in oak woodlands in California and elsewhere.

Conserving the evolutionary potential of California valley oak (Quercus lobata Née): a multivariate genetic approach to conservation planning

California valley oak (Quercus lobata Née) is a seriously threatened endemic oak species in California and a keystone species for foothill oak ecosystems. Urban and agricultural development affects a significant fraction of the species' range and predicted climate change is likely to dislocate many current populations. Here, we explore spatial patterns of multivariate genotypes and genetic diversity throughout the range of valley oak to determine whether ongoing and future patterns of habitat loss could threaten the evolutionary potential of the species by eradicating populations of distinctive genetic composition. This manuscript will address three specific questions: (i) What is the spatial genetic structure of the chloroplast and nuclear genetic markers? (ii) What are the geographical trends in the distribution of chloroplast and nuclear genotypes? (iii) Is there any part of the species' range where allelic diversity in either the chloroplast or nuclear genomes is particularly high? We analysed six chloroplast and seven nuclear microsatellite genetic markers of individuals widespread across the valley oak range. We then used a multivariate approach correlating genetic markers and geographical variables through a canonical trend surface analysis, followed by GIS mapping of the significant axes. We visualized population allelic richness spatially with GIS tools to identify regions of high diversity. Our findings, based on the distribution of multivariate genotypes and allelic richness, identify areas with distinctive histories and genetic composition that should be given priority in reserve network design, especially because these areas also overlap with landscape change and little degree of protection. Thus, without a careful preservation plan, valuable evolutionary information will be lost for valley oak.