Purpose of the study. Comprehensive assessment of the clinical and immunological changes in cervical epithelium and the vaginal microbiota in patients with cervical intraepithelial neoplasia (CIN) II-III stage after radiosurgical conization and the drug Neo-Penotran Forte L.Materials and methods. The study included 2 groups of sexually active women (n=143) aged 21 to 45 years (mean age - 34.5±6.5 years). They were examined for the presence of genital infections and vector-borne, liquid-based cytology, colposcopy. All patients were treated with radiosurgery method in the amount of conization.Results. Patients treated in the pre - and postoperative period with Neo-Forte Penotran A, were found to have a marked reduction of proinflammatory and anti-inflammatory cytokines increase. Median values analysis has allowed to ascertain the prevalence ofinterleukin indicators (IL)-1a and IL-b in the 2nd group over those in the 1st, when U (Mann-Whitney test)=5.9 and U=7.5, respectively, those p *fcr. Conclusions.Use of the drug Neo-Penotran Forte L affects the key pathophysiological processes of septic complications, prevents their development and contributes to improved tissue repair in patients after radiosurgical treatment. An integrated pre - and post-operative treatment helps to improve the immune status of the vaginal microecology.